MD14G1008000.v1.1

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009860
Physical Location & Seq
Reverse (-)
799695 .. 817208
17514 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1008000.v1.1.491

Sequence Viewer

Length: 2781 bp
ATGTTGGCAAATCCTGACAGTGGCAGACTCCACTGCTCTAATCCCTCCCAATTCCTGAACCCAAACAGGCCCAAATGTTTCCGCTTTCCAAACTGCCCTCGTTCCCCATGCTTCCCCAGCAAGTCCAAGTTCTGCAACTTGTCCCTGCTTCTGCCCAGGCAGACCAGCAAGAAGGGTCTTGGCATCAGAGCTTTGTCCGATCCCGGCGGTGTTTCGACGAGCGGTGGTCCTCTGGGATACCCTCAGCCGTACTCTGTCAAAATCCCTGTTGGTGATAGACATATTTTGGTTGAGACAGGTCATATTGGGAGACAAGCCAGTGCTTCTGTTACAGTGACAGATGGAGAAACCATTATTTACACATCTGTTTGTCTGGCTGACACTCCAAGTGAGCCTTCAGACTTCTTCCCTCTTTCAGTACATTATCAGGAGCGCTTTTCTGCAGCAGGTCGAACTAGTGGAGGCTTCTTCAAACGAGAAGGAAGGGCAAAAGATCATGAGGTTCTCATTTGTAGATTGATTGATAGGCCTTTGCGTCCAACTATGTTGAACGGCTTCTACCATGAAACTCAGATACTTTCTTGGGTTCTGAGTTATGATGGTTTGCACTCTCCTGATTCTTTGGCAGTCACAGCAGCTGGGATAGCAGTGGCTCTTTCAGAAGTTCCAAACTCAAAAACTGTAGCAGGAGTGCGAATTGGTCTTGTTGGTGACAAATTTATCGTGAATCCAACAATAAAGGAGATGGAAGACTCTGAATTGGACTTGTTGGTCGCTGGCACGGATAATGCAATATTGATGATAGAGGGCTATTGTGATTTTCTCACAGAGGAAAAGTTGCTGCAAGCAGTAGAAGTTGGGCAGGATGCCATACGAGCAATTTGCAATGAGGTAGAAGCTTTGGTTAAGAAGTGTGGAAAGCCAAAAATGCTTGATGCAATCAAATTACCTCCCCCTGAGCTATATAATCATGTTGAGGAAATTGCCGGTGATGAGTTGGTAAGAGCTCTGCAGATTAAGGACAAGATTCCTAGAAGGAAAGCTCTCTTGTCTTTGGAAGGAGAAGTTTTAACTATACTAACTGAAAAGGGATATATTAGTAAGGATGCAGCTCTCATAGGCAGTGAAACAATACCAGATATATTTGAAGATGAAGAGGAGGATGAGGAAGTGGTTGTGGATGGTGAAGTAGATGAAGGTGATGTTCATATAAAGCCTAGTTCACGAAAGTCAACCCCCTTGTTCTTTTCTGAGGTAGATGTGAAGCTGGTATTCAAAGAAGTTACATCCAAATTTTTACGAAGACGCATTGTGGAGGGTGGAAGAAGAAGTGATGGACGTACCCCATATGAGATACGTCCAATTAACTCAAAATGTGGACTGCTTCCAAGGGCACATGGAAGTGCTCTTTTCACACGTGGGGAAACCCAGTCATTGGCTGTTGTTACACTCGGGGATAAACAGATGGCACAGAGGGTAGACAACCTAGTTGATGTTGACGAATGGAAGAGATTCTATCTCCAGTACACATTTCCCCCTTCATGTGTTGGGGAAGTTGGACGGAATGGAGCCCCTAGTAGAAGAGAAATTGGCCATGGAATGCTTGCTGAGAGAGCTCTTGAACCAATTTTGCCTTCCGAAGATGATTTTCCTTACACTATACGTGTTGAGAGTACAATTACTGAAAGCAATGGCTCTTCAAGTATGGCATCTGTTTGTGGGGGCTGCTTAGCTTTGCAGGATGCTGGTGTTCCGATCAAGTCCTCTATAGTTGGGATAGCAATGGGTATGGTTCTGGAAACTGAGGAATTTGGGGGAAACGGTTCTCCACTAATTCTGTCTGACATAACTGGATCTGAAGATGCATCTGGGGATATGGATTTCAAGGTTGCTGGAAATGAAGATGGTGTAACTGCATTTCAGATGGACATAAAGGTGAGCGGGATTACTTTACCAGTTATGAGAGAGGCACTCCTACAGGCAAAGGATGGCCGTAAGCATATTATTGCGGAAATGCTGAAGTGCTCATCTCCTCCTTCAAGATCGCTTTCTAAGTACGCACCACTGATTCACATAATGAAGGTCAACCCCGAGAAAGTAAGCTTAATCATTGGTAGTGGTGGGAAGAAGGTGAAGAGTATTATAGAAGAGTCTGGAGTAGACGCAATTGACACACAAGAAGACGGGATAGTGAGAATTACCGGCAGGGATTTGTCTAGCATAGAAAAGACTAAAGCTATTATTACTAACTTGACAATGGTTCCGTCAGTTGGTGATATCTTCAGGAACTGCGAGATCAAATCAATAGTTCCATATGGAGTATTTGTGGAAATAGCACCTGGGCGAGAGGGACTTTGCCATATTAGCGAGTTGAGTTCAGAGTGGCTGGCAAAGGCAGAAGACGCTGTTAAAGTTGGACAGCATATTGATGTTAAACTTATTGAGGTAAATGAGAAGGGGCAACTTCGCCTTAGCCGCAAAGCTCTACTTCCAGATACAGATGCAGAGAATGCCAGTTCAAAGCAGCCTACGGGTGACTCCAGTGATATAGCTTCCCAAGAAACATCTGACAAGGGTTCAACGAAGAAAATTGTTAGCACTCCAAAGAGGGACTTAGCTGGAGAGCTCCCACAAAAGAAGTTCATTCGGAGATTAGTCAGTGCTGCCAAAGACAGACCAAATATCAACAAAGATAAGACAAAGAAGAGCAGTAAAGTAGTCAGTAGCGTCTCCAGCAAGGACGAAAATACTTCAGTGAATGGAGAGGCTAACATTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

927

Amino Acids

100.51

Weight (kDa)

5.69

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 93 - 223 1.6e-23 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 227 - 289 1.3e-15 3' exoribonuclease family, domain 2
RNase_PH PF01138 451 - 585 2.3e-29 3' exoribonuclease family, domain 1
KH_1 PF00013 692 - 749 1e-07 KH domain
S1 PF00575 765 - 827 4e-12 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
Acc36I ACCTGC 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 1435
AccBSI CCGCTC 2 cut(s) 222, 1941
AccI GTMKAC 2 cut(s) 1479, 2160
AciI CCGC 6 cut(s) 82, 207, 222, 1941, 2009, 2476
AclWI GGATC 2 cut(s) 194, 1861
AcoI YGGCCR 2 cut(s) 1591, 1990
AcsI RAATTY 3 cut(s) 716, 1292, 1808
AcuI CTGAAG 5 cut(s) 381, 1878, 2039, 2266, 2736
AcvI CACGTG 1 cut(s) 1418
AfaI GTAC 6 cut(s) 251, 420, 1342, 1526, 1675, 2057
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 20, 1435, 2270
AflIII ACRYGT 2 cut(s) 1415, 1663
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 2 cut(s) 155, 2338
AjuI GAANNNNNNNTTGG 4 cut(s) 379, 411, 1618, 1650
Alw21I GWGCWC 5 cut(s) 1009, 1408, 1618, 2027, 2628
Alw26I GTCTC 3 cut(s) 287, 304, 2734
AlwI GGATC 2 cut(s) 194, 1861
AlwNI CAGNNNCTG 2 cut(s) 638, 2289
Ama87I CYCGRG 2 cut(s) 1451, 2090
Aor51HI AGCGCT 1 cut(s) 434
AoxI GGCC 4 cut(s) 68, 527, 1591, 1990
ApeKI GCWGC 7 cut(s) 443, 635, 841, 1109, 1725, 2524, 2663
ApoI RAATTY 3 cut(s) 716, 1292, 1808
Asp700I GAANNNNTTC 3 cut(s) 554, 1511, 1822
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 69, 227
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 9 cut(s) 284, 722, 1001, 1196, 1211, 1948, 2143, 2285, 2546
AvaI CYCGRG 2 cut(s) 1451, 2090
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 1396
BalI TGGCCA 1 cut(s) 1593
BanII GRGCYC 4 cut(s) 1009, 1573, 1618, 2628
BbrPI CACGTG 1 cut(s) 1418
BbsI GAAGAC 4 cut(s) 756, 1309, 2187, 2406
Bbv12I GWGCWC 5 cut(s) 1009, 1408, 1618, 2027, 2628
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 7 cut(s) 455, 647, 828, 1121, 1712, 2536, 2650
BccI CCATC 9 cut(s) 335, 593, 739, 1175, 1328, 1459, 1898, 1918, 1982
BceAI ACGGC 3 cut(s) 232, 568, 1977
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciT130I CCWGG 2 cut(s) 157, 2340
BciVI GTATCC 1 cut(s) 230
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 287, 304, 2734
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 6 cut(s) 456, 1032, 1218, 1487, 1575, 2217
BfmI CTRYAG 5 cut(s) 441, 681, 1010, 1767, 1976
BfoI RGCGCY 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 1 cut(s) 230
BisI GCNGC 8 cut(s) 444, 636, 842, 1110, 1726, 2476, 2525, 2664
BlpI GCTNAGC 1 cut(s) 1729
BlsI GCNGC 8 cut(s) 445, 637, 843, 1111, 1727, 2477, 2526, 2665
Bme1390I CCNGG 3 cut(s) 157, 204, 2340
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 1451, 2090
BmgT120I GGNCC 2 cut(s) 69, 227
BmiI GGNNCC 2 cut(s) 1570, 2262
BmrFI CCNGG 3 cut(s) 157, 204, 2340
BmrI ACTGGG 1 cut(s) 1423
BmsI GCATC 9 cut(s) 192, 856, 925, 1096, 1718, 1732, 1852, 1874, 2491
BmuI ACTGGG 1 cut(s) 1423
BpiI GAAGAC 4 cut(s) 756, 1309, 2187, 2406
BplI GAGNNNNNCTC 2 cut(s) 1956, 1988
BpmI CTGGAG 5 cut(s) 1505, 2175, 2524, 2640, 2716
Bpu10I CCTNAGC 3 cut(s) 243, 957, 2471
Bpu1102I GCTNAGC 1 cut(s) 1729
BpuMI CCSGG 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 1418, 1664
BsaJI CCNNGG 4 cut(s) 155, 1388, 1594, 2339
BsaXI ACNNNNNCTCC 2 cut(s) 2310, 2340
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 1435, 2270
Bse118I RCCGGY 2 cut(s) 986, 2201
Bse1I ACTGG 7 cut(s) 318, 1429, 1522, 1855, 1955, 2514, 2541
Bse3DI GCAATG 3 cut(s) 892, 1696, 1788
BseBI CCWGG 2 cut(s) 157, 2340
BseDI CCNNGG 4 cut(s) 155, 1388, 1594, 2339
BseGI GGATG 7 cut(s) 871, 1111, 1168, 1186, 1286, 1747, 1993
BseLI CCNNNNNNNGG 3 cut(s) 20, 1435, 2270
BseMI GCAATG 3 cut(s) 892, 1696, 1788
BseMII CTCAG 7 cut(s) 257, 581, 584, 948, 1242, 1599, 1794
BseNI ACTGG 7 cut(s) 318, 1429, 1522, 1855, 1955, 2514, 2541
BseRI GAGGAG 2 cut(s) 1172, 2022
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 7 cut(s) 455, 647, 828, 1121, 1712, 2536, 2650
BseYI CCCAGC 2 cut(s) 116, 638
BshFI GGCC 4 cut(s) 70, 529, 1593, 1992
BsiHKAI GWGCWC 5 cut(s) 1009, 1408, 1618, 2027, 2628
BsiHKCI CYCGRG 2 cut(s) 1451, 2090
BsiSI CCGG 3 cut(s) 204, 987, 2202
BslFI GGGAC 3 cut(s) 127, 2364, 2624
BslI CCNNNNNNNGG 3 cut(s) 20, 1435, 2270
BsmAI GTCTC 3 cut(s) 287, 304, 2734
BsmBI CGTCTC 1 cut(s) 2734
BsmFI GGGAC 3 cut(s) 127, 2364, 2624
BsmI GAATGC 2 cut(s) 1605, 2515
BsnI GGCC 4 cut(s) 70, 529, 1593, 1992
BsoBI CYCGRG 2 cut(s) 1451, 2090
Bsp1286I GDGCHC 7 cut(s) 1009, 1396, 1408, 1573, 1618, 2027, 2628
Bsp143I GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
Bsp1720I GCTNAGC 1 cut(s) 1729
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 6 cut(s) 82, 207, 222, 1941, 2009, 2476
BspANI GGCC 4 cut(s) 70, 529, 1593, 1992
BspCNI CTCAG 7 cut(s) 256, 582, 583, 949, 1243, 1600, 1795
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 1570, 2262
BspMAI CTGCAG 2 cut(s) 445, 1014
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 2 cut(s) 194, 1861
BspQI GCTCTTC 2 cut(s) 1702, 2699
BsrBI CCGCTC 2 cut(s) 222, 1941
BsrDI GCAATG 3 cut(s) 892, 1696, 1788
BsrFI RCCGGY 2 cut(s) 986, 2201
BsrI ACTGG 7 cut(s) 318, 1429, 1522, 1855, 1955, 2514, 2541
BssAI RCCGGY 2 cut(s) 986, 2201
BssECI CCNNGG 4 cut(s) 155, 1388, 1594, 2339
BssMI GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
BssT1I CCWWGG 2 cut(s) 1388, 1594
Bst2UI CCWGG 2 cut(s) 157, 2340
Bst4CI ACNGT 4 cut(s) 20, 334, 682, 1823
Bst6I CTCTTC 7 cut(s) 1149, 1502, 1576, 1702, 2129, 2142, 2699
BstAPI GCANNNNNTGC 1 cut(s) 2510
BstBAI YACGTR 2 cut(s) 1418, 1664
BstC8I GCNNGC 4 cut(s) 778, 846, 1605, 2388
BstDSI CCRYGG 1 cut(s) 1594
BstF5I GGATG 7 cut(s) 871, 1111, 1168, 1186, 1286, 1747, 1993
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 6 cut(s) 202, 496, 1758, 1856, 2045, 2298
BstMAI GTCTC 3 cut(s) 287, 304, 2734
BstMBI GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
BstNI CCWGG 2 cut(s) 157, 2340
BstSCI CCNGG 3 cut(s) 155, 202, 2338
BstSFI CTRYAG 5 cut(s) 441, 681, 1010, 1767, 1976
BstSLI GKGCMC 1 cut(s) 1396
BstV1I GCAGC 7 cut(s) 455, 647, 828, 1121, 1712, 2536, 2650
BstV2I GAAGAC 4 cut(s) 756, 1309, 2187, 2406
BstX2I RGATCY 1 cut(s) 1853
BstYI RGATCY 1 cut(s) 1853
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 4 cut(s) 70, 529, 1593, 1992
BtgI CCRYGG 1 cut(s) 1594
BtsCI GGATG 7 cut(s) 871, 1111, 1168, 1186, 1286, 1747, 1993
BtsI GCAGTG 3 cut(s) 31, 654, 1129
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 4 cut(s) 778, 846, 1605, 2388
CaiI CAGNNNCTG 2 cut(s) 638, 2289
CciI TCATGA 1 cut(s) 496
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 2 cut(s) 986, 2201
Cfr13I GGNCC 2 cut(s) 69, 227
CseI GACGC 5 cut(s) 524, 1314, 2171, 2411, 2716
Csp6I GTAC 6 cut(s) 250, 419, 1341, 1525, 1674, 2056
CviAII CATG 7 cut(s) 108, 497, 563, 971, 1397, 1542, 1595
CviQI GTAC 6 cut(s) 250, 419, 1341, 1525, 1674, 2056
DpnI GATC 6 cut(s) 201, 495, 1757, 1855, 2044, 2297
DpnII GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 2 cut(s) 1591, 1990
Eam1104I CTCTTC 7 cut(s) 1149, 1502, 1576, 1702, 2129, 2142, 2699
EarI CTCTTC 7 cut(s) 1149, 1502, 1576, 1702, 2129, 2142, 2699
Ecl136II GAGCTC 3 cut(s) 1007, 1616, 2626
Eco130I CCWWGG 2 cut(s) 1388, 1594
Eco147I AGGCCT 1 cut(s) 529
Eco24I GRGCYC 4 cut(s) 1009, 1573, 1618, 2628
Eco32I GATATC 1 cut(s) 2278
Eco47I GGWCC 1 cut(s) 227
Eco47III AGCGCT 1 cut(s) 434
Eco53kI GAGCTC 3 cut(s) 1007, 1616, 2626
Eco57I CTGAAG 5 cut(s) 381, 1878, 2039, 2266, 2736
Eco72I CACGTG 1 cut(s) 1418
Eco88I CYCGRG 2 cut(s) 1451, 2090
EcoICRI GAGCTC 3 cut(s) 1007, 1616, 2626
EcoRII CCWGG 2 cut(s) 155, 2338
EcoRV GATATC 1 cut(s) 2278
EcoT14I CCWWGG 2 cut(s) 1388, 1594
EcoT22I ATGCAT 1 cut(s) 1867
EcoT38I GRGCYC 4 cut(s) 1009, 1573, 1618, 2628
ErhI CCWWGG 2 cut(s) 1388, 1594
Esp3I CGTCTC 1 cut(s) 2734
FaeI CATG 7 cut(s) 111, 500, 566, 974, 1400, 1545, 1598
FalI AAGNNNNNCTT 6 cut(s) 379, 411, 2472, 2504, 2597, 2629
FaqI GGGAC 3 cut(s) 127, 2364, 2624
FatI CATG 7 cut(s) 107, 496, 562, 970, 1396, 1541, 1594
FauI CCCGC 1 cut(s) 1934
FauNDI CATATG 2 cut(s) 1348, 2314
FblI GTMKAC 2 cut(s) 1479, 2160
Fnu4HI GCNGC 8 cut(s) 444, 636, 842, 1110, 1726, 2476, 2525, 2664
FokI GGATG 7 cut(s) 878, 1118, 1175, 1193, 1273, 1754, 2000
FriOI GRGCYC 4 cut(s) 1009, 1573, 1618, 2628
Fsp4HI GCNGC 8 cut(s) 444, 636, 842, 1110, 1726, 2476, 2525, 2664
FspBI CTAG 6 cut(s) 456, 1032, 1218, 1487, 1575, 2217
GlaI GCGC 1 cut(s) 434
GluI GCNGC 8 cut(s) 444, 636, 842, 1110, 1726, 2476, 2525, 2664
GsaI CCCAGC 2 cut(s) 120, 642
GsuI CTGGAG 5 cut(s) 1505, 2175, 2524, 2640, 2716
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 4 cut(s) 70, 529, 1593, 1992
HapII CCGG 3 cut(s) 204, 987, 2202
HgaI GACGC 5 cut(s) 524, 1314, 2171, 2411, 2716
HhaI GCGC 1 cut(s) 435
Hin1II CATG 7 cut(s) 111, 500, 566, 974, 1400, 1545, 1598
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HincII GTYRAC 3 cut(s) 1233, 1498, 2086
HindII GTYRAC 3 cut(s) 1233, 1498, 2086
HindIII AAGCTT 2 cut(s) 897, 2101
HinfI GANTC 9 cut(s) 27, 617, 727, 752, 1027, 1512, 2068, 2150, 2537
HpaII CCGG 3 cut(s) 204, 987, 2202
HphI GGTGA 9 cut(s) 284, 722, 1001, 1196, 1211, 1948, 2143, 2285, 2546
Hpy166II GTNNAC 8 cut(s) 1223, 1233, 1379, 1480, 1498, 1527, 2086, 2161
Hpy8I GTNNAC 8 cut(s) 1223, 1233, 1379, 1480, 1498, 1527, 2086, 2161
Hpy99I CGWCG 1 cut(s) 220
HpyCH4III ACNGT 4 cut(s) 20, 334, 682, 1823
HpyCH4IV ACGT 4 cut(s) 1339, 1357, 1417, 1663
HpySE526I ACGT 4 cut(s) 1339, 1357, 1417, 1663
Hsp92II CATG 7 cut(s) 111, 500, 566, 974, 1400, 1545, 1598
HspAI GCGC 1 cut(s) 433
Kzo9I GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
LguI GCTCTTC 2 cut(s) 1702, 2699
LmnI GCTCC 3 cut(s) 430, 1568, 2631
Lsp1109I GCAGC 7 cut(s) 455, 647, 828, 1121, 1712, 2536, 2650
LweI GCATC 9 cut(s) 192, 856, 925, 1096, 1718, 1732, 1852, 1874, 2491
MaeI CTAG 6 cut(s) 456, 1032, 1218, 1487, 1575, 2217
MaeII ACGT 4 cut(s) 1339, 1357, 1417, 1663
MaeIII GTNAC 8 cut(s) 328, 334, 628, 710, 1282, 1444, 1909, 2534
MalI GATC 6 cut(s) 201, 495, 1757, 1855, 2044, 2297
MbiI CCGCTC 2 cut(s) 222, 1941
MboI GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
MfeI CAATTG 1 cut(s) 2166
MflI RGATCY 1 cut(s) 1853
MhlI GDGCHC 7 cut(s) 1009, 1396, 1408, 1573, 1618, 2027, 2628
MlsI TGGCCA 1 cut(s) 1593
MluNI TGGCCA 1 cut(s) 1593
MlyI GAGTC 4 cut(s) 21, 746, 2159, 2531
MmeI TCCRAC 4 cut(s) 563, 755, 1537, 2395
Mox20I TGGCCA 1 cut(s) 1593
Mph1103I ATGCAT 1 cut(s) 1867
MroXI GAANNNNTTC 3 cut(s) 554, 1511, 1822
MscI TGGCCA 1 cut(s) 1593
MseI TTAA 7 cut(s) 906, 1017, 1070, 1365, 2105, 2409, 2433
MslI CAYNNNNRTG 3 cut(s) 1401, 1934, 2427
Msp20I TGGCCA 1 cut(s) 1593
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 3 cut(s) 204, 987, 2202
MspR9I CCNGG 3 cut(s) 157, 204, 2340
MunI CAATTG 1 cut(s) 2166
Mva1269I GAATGC 2 cut(s) 1605, 2515
MvaI CCWGG 2 cut(s) 157, 2340
NciI CCSGG 1 cut(s) 204
NcoI CCATGG 1 cut(s) 1594
NdeI CATATG 2 cut(s) 1348, 2314
NdeII GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
NlaIII CATG 7 cut(s) 111, 500, 566, 974, 1400, 1545, 1598
NlaIV GGNNCC 2 cut(s) 1570, 2262
NmuCI GTSAC 4 cut(s) 334, 628, 710, 2534
NsiI ATGCAT 1 cut(s) 1867
PagI TCATGA 1 cut(s) 496
PceI AGGCCT 1 cut(s) 529
PciSI GCTCTTC 2 cut(s) 1702, 2699
PctI GAATGC 2 cut(s) 1605, 2515
PdmI GAANNNNTTC 3 cut(s) 554, 1511, 1822
PfeI GAWTC 5 cut(s) 617, 727, 1027, 1512, 2068
PflMI CCANNNNNTGG 1 cut(s) 1435
PkrI GCNGC 8 cut(s) 445, 637, 843, 1111, 1727, 2477, 2526, 2665
PleI GAGTC 4 cut(s) 21, 746, 2158, 2531
PmaCI CACGTG 1 cut(s) 1418
PmlI CACGTG 1 cut(s) 1418
PpsI GAGTC 4 cut(s) 21, 746, 2158, 2531
Ppu21I YACGTR 2 cut(s) 1418, 1664
Psp124BI GAGCTC 3 cut(s) 1009, 1618, 2628
Psp6I CCWGG 2 cut(s) 155, 2338
PspCI CACGTG 1 cut(s) 1418
PspFI CCCAGC 2 cut(s) 116, 638
PspGI CCWGG 2 cut(s) 155, 2338
PspN4I GGNNCC 2 cut(s) 1570, 2262
PspPI GGNCC 2 cut(s) 69, 227
PsrI GAACNNNNNNTAC 2 cut(s) 542, 574
PstI CTGCAG 2 cut(s) 445, 1014
PstNI CAGNNNCTG 2 cut(s) 638, 2289
PsuI RGATCY 1 cut(s) 1853
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 6 cut(s) 251, 420, 1342, 1526, 1675, 2057
RsaNI GTAC 6 cut(s) 250, 419, 1341, 1525, 1674, 2056
RseI CAYNNNNRTG 3 cut(s) 1401, 1934, 2427
SacI GAGCTC 3 cut(s) 1009, 1618, 2628
SapI GCTCTTC 2 cut(s) 1702, 2699
SaqAI TTAA 7 cut(s) 906, 1017, 1070, 1365, 2105, 2409, 2433
SatI GCNGC 8 cut(s) 444, 636, 842, 1110, 1726, 2476, 2525, 2664
Sau3AI GATC 6 cut(s) 199, 493, 1755, 1853, 2042, 2295
Sau96I GGNCC 2 cut(s) 69, 227
SchI GAGTC 4 cut(s) 21, 746, 2159, 2531
ScrFI CCNGG 3 cut(s) 157, 204, 2340
SduI GDGCHC 7 cut(s) 1009, 1396, 1408, 1573, 1618, 2027, 2628
SfaNI GCATC 9 cut(s) 192, 856, 925, 1096, 1718, 1732, 1852, 1874, 2491
SfcI CTRYAG 5 cut(s) 441, 681, 1010, 1767, 1976
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 3 cut(s) 1401, 1934, 2427
SpeI ACTAGT 1 cut(s) 455
SseBI AGGCCT 1 cut(s) 529
SsiI CCGC 6 cut(s) 82, 207, 222, 1941, 2009, 2476
SspI AATATT 1 cut(s) 795
SspMI CTAG 6 cut(s) 456, 1032, 1218, 1487, 1575, 2217
SstI GAGCTC 3 cut(s) 1009, 1618, 2628
StuI AGGCCT 1 cut(s) 529
StyD4I CCNGG 3 cut(s) 155, 202, 2338
StyI CCWWGG 2 cut(s) 1388, 1594
TaaI ACNGT 4 cut(s) 20, 334, 682, 1823
TaiI ACGT 4 cut(s) 1342, 1360, 1420, 1666
TaqI TCGA 2 cut(s) 215, 451
TatI WGTACW 3 cut(s) 418, 1524, 1673
TauI GCSGC 1 cut(s) 2478
TfiI GAWTC 5 cut(s) 617, 727, 1027, 1512, 2068
Tru1I TTAA 7 cut(s) 906, 1017, 1070, 1365, 2105, 2409, 2433
Tru9I TTAA 7 cut(s) 906, 1017, 1070, 1365, 2105, 2409, 2433
TseFI GTSAC 4 cut(s) 334, 628, 710, 2534
TseI GCWGC 7 cut(s) 443, 635, 841, 1109, 1725, 2524, 2663
Tsp45I GTSAC 4 cut(s) 334, 628, 710, 2534
TspDTI ATGAA 8 cut(s) 579, 1167, 1196, 1209, 1530, 1914, 2093, 2632
TspGWI ACGGA 3 cut(s) 797, 1576, 2253
Van91I CCANNNNNTGG 1 cut(s) 1435
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 3 cut(s) 716, 1292, 1808
XmiI GTMKAC 2 cut(s) 1479, 2160
XmnI GAANNNNTTC 3 cut(s) 554, 1511, 1822
XspI CTAG 6 cut(s) 456, 1032, 1218, 1487, 1575, 2217
Zsp2I ATGCAT 1 cut(s) 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.