pycom12g00890

polyribonucleotide nucleotidyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009860
Physical Location & Seq
Reverse (-)
743655 .. 748122
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00890.1

Sequence Viewer

Length: 987 bp
ATGGCACAGAGGTTAGACAACCTAGTAGGTGATGACGAATGGAAGAGATTCTATCTCCAGTACACATTTCCCCCTTCTTGTGTTGGGGAAGTTGGACGGACTGGAGCCCCTAGTAGAAGAGAAATTGGCCATGGAATGCTTGCTGAGAGAGCTCTTGAACCAATTTTGCCTTCCGAAGATGATTTTCCTTACACTATACGTGTCGAGAGTACAATTACTGAAAGCAATGGCTCTTCAAGTATGGCATCTGTTTGTGGGGGCTGCTTAGCTTTGCAGGATGCTGGGGTTCCAATCAAGTCCTCTATAGTTGGGATAGCAATGGGTATGGTTTTGGAAACTGAAGAATTTGGGGGAAATGGTGCTCCACTAATTCTGTCTGACATAACTGGATCTGAAGATGCATCCGGGGATATGGATTTCAAGGTTGCTGGAAATGAAGATGGTGTAACTGCATTTCAGATGGACATAAAGGTGAGCGGGATTACATTACCAGTTATGAGAGAGGCACTCCTACAGGCAAAGGATGGCCGGAAGCATATTATTGCGGAAATGTTGAAGTGCTCACCTCCTCCTTCAAGATCGCTTTCTATGTATGCACCACTGATTCACATAATGAAGGTCAACCCTGAGAAAGTAAACTTAATCATTGGTAGTGGTGGGAAGAAGGTGAAGAGTATTATAGAAGAGAGTGGAGTAGATGCCATCGACACACAAGATGACGGGATTGTGAAAATTACTGGCAGGGATTTGTCTAGCATAGAAAAGTCTAAAGCTATTATTACGAACTTGACAATGGTTCCGTCAGTTGGCGATATCTTCAGGAACTGCGAGATCAAATCAATAGTTCCATATGGAGTATTCGTGGAAATAGCACCTGGGCGAGAGGGACTTTGCCATATTAGCGAGTTGACTTCAGAGTGGCTGGCCAAGGCAGAAGATGCTGTTAAAGATGGATCGATCATATTGATGTTAAACTTATTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000175 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0004654 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006109 GO:0006139 GO:0006396 GO:0006401 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008299 GO:0008408 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010322 GO:0010323 GO:0010467 GO:0010563 GO:0010565 GO:0010675 GO:0010677 GO:0015994 GO:0015995 GO:0016036 GO:0016070 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016122 GO:0016123 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0018130 GO:0019216 GO:0019220 GO:0019222 GO:0019438 GO:0019439 GO:0019747 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031425 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033014 GO:0033554 GO:0034641 GO:0034655 GO:0042214 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045827 GO:0045833 GO:0045912 GO:0045936 GO:0046148 GO:0046246 GO:0046483 GO:0046700 GO:0046890 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051174 GO:0051186 GO:0051188 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0071071 GO:0071072 GO:0071496 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1903725 GO:1903726
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko03018 map00230 map00240 map03018
Pfam Domains
Protein Families
KH_1 PNPase RNase_PH RNase_PH_C S1

Protein Analysis

329

Amino Acids

35.22

Weight (kDa)

4.84

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 13 - 97 2.3e-17 3' exoribonuclease family, domain 1
KH_1 PF00013 204 - 261 3.5e-08 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 477
AciI CCGC 2 cut(s) 477, 545
AclWI GGATC 2 cut(s) 397, 961
AcoI YGGCCR 3 cut(s) 127, 526, 924
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 4 cut(s) 360, 414, 802, 897
AfaI GTAC 2 cut(s) 62, 211
AfiI CCNNNNNNNGG 1 cut(s) 806
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 5 cut(s) 158, 237, 421, 556, 576
AjnI CCWGG 1 cut(s) 874
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
AluBI AGCT 3 cut(s) 152, 269, 773
AluI AGCT 3 cut(s) 152, 269, 773
Alw21I GWGCWC 3 cut(s) 154, 364, 563
AlwI GGATC 2 cut(s) 397, 961
AlwNI CAGNNNCTG 1 cut(s) 825
AoxI GGCC 3 cut(s) 127, 526, 924
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 4 cut(s) 41, 484, 555, 679
BalI TGGCCA 2 cut(s) 129, 926
BanII GRGCYC 2 cut(s) 109, 154
Bbv12I GWGCWC 3 cut(s) 154, 364, 563
BbvI GCAGC 1 cut(s) 248
BccI CCATC 5 cut(s) 434, 454, 518, 710, 944
BciT130I CCWGG 1 cut(s) 876
BcnI CCSGG 1 cut(s) 406
BfaI CTAG 3 cut(s) 23, 111, 753
BfmI CTRYAG 2 cut(s) 303, 512
BisI GCNGC 1 cut(s) 262
BlpI GCTNAGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 2 cut(s) 406, 876
BmiI GGNNCC 3 cut(s) 106, 288, 798
BmrFI CCNGG 2 cut(s) 406, 876
BmsI GCATC 6 cut(s) 254, 268, 388, 410, 688, 928
BplI GAGNNNNNCTC 2 cut(s) 492, 524
BpmI CTGGAG 2 cut(s) 41, 123
Bpu1102I GCTNAGC 1 cut(s) 265
BpuMI CCSGG 1 cut(s) 406
Bsa29I ATCGAT 1 cut(s) 956
BsaAI YACGTR 1 cut(s) 200
BsaJI CCNNGG 4 cut(s) 130, 405, 875, 927
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bsc4I CCNNNNNNNGG 1 cut(s) 806
Bse1I ACTGG 5 cut(s) 58, 106, 391, 491, 742
Bse3DI GCAATG 2 cut(s) 232, 324
BseBI CCWGG 1 cut(s) 876
BseCI ATCGAT 1 cut(s) 956
BseDI CCNNGG 4 cut(s) 130, 405, 875, 927
BseGI GGATG 3 cut(s) 283, 401, 529
BseLI CCNNNNNNNGG 1 cut(s) 806
BseMI GCAATG 2 cut(s) 232, 324
BseMII CTCAG 2 cut(s) 135, 618
BseNI ACTGG 5 cut(s) 58, 106, 391, 491, 742
BseRI GAGGAG 1 cut(s) 558
BseXI GCAGC 1 cut(s) 248
BseYI CCCAGC 1 cut(s) 281
BshFI GGCC 3 cut(s) 129, 528, 926
BshVI ATCGAT 1 cut(s) 956
BsiHKAI GWGCWC 3 cut(s) 154, 364, 563
BsiSI CCGG 2 cut(s) 405, 529
BslFI GGGAC 1 cut(s) 900
BslI CCNNNNNNNGG 1 cut(s) 806
BsmFI GGGAC 1 cut(s) 900
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 3 cut(s) 129, 528, 926
Bsp1286I GDGCHC 4 cut(s) 109, 154, 364, 563
Bsp143I GATC 5 cut(s) 389, 578, 831, 953, 957
Bsp1720I GCTNAGC 1 cut(s) 265
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 2 cut(s) 477, 545
BspANI GGCC 3 cut(s) 129, 528, 926
BspCNI CTCAG 2 cut(s) 136, 619
BspDI ATCGAT 1 cut(s) 956
BspLI GGNNCC 3 cut(s) 106, 288, 798
BspPI GGATC 2 cut(s) 397, 961
BspQI GCTCTTC 1 cut(s) 238
BsrBI CCGCTC 1 cut(s) 477
BsrDI GCAATG 2 cut(s) 232, 324
BsrI ACTGG 5 cut(s) 58, 106, 391, 491, 742
BssECI CCNNGG 4 cut(s) 130, 405, 875, 927
BssMI GATC 5 cut(s) 389, 578, 831, 953, 957
BssT1I CCWWGG 2 cut(s) 130, 927
Bst2UI CCWGG 1 cut(s) 876
Bst6I CTCTTC 5 cut(s) 38, 112, 238, 665, 678
BstAPI GCANNNNNTGC 1 cut(s) 938
BstBAI YACGTR 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 141, 924
BstDEI CTNAG 3 cut(s) 144, 265, 627
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 3 cut(s) 283, 401, 529
BstKTI GATC 5 cut(s) 392, 581, 834, 956, 960
BstMBI GATC 5 cut(s) 389, 578, 831, 953, 957
BstMWI GCNNNNNNNGC 3 cut(s) 149, 900, 938
BstNI CCWGG 1 cut(s) 876
BstSCI CCNGG 2 cut(s) 404, 874
BstSFI CTRYAG 2 cut(s) 303, 512
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
Bsu15I ATCGAT 1 cut(s) 956
BsuRI GGCC 3 cut(s) 129, 528, 926
BsuTUI ATCGAT 1 cut(s) 956
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 3 cut(s) 283, 401, 529
BtsIMutI CAGTG 1 cut(s) 599
Cac8I GCNNGC 2 cut(s) 141, 924
CaiI CAGNNNCTG 1 cut(s) 825
ClaI ATCGAT 1 cut(s) 956
Csp6I GTAC 2 cut(s) 61, 210
CviAII CATG 1 cut(s) 131
CviQI GTAC 2 cut(s) 61, 210
DdeI CTNAG 3 cut(s) 144, 265, 627
DpnI GATC 5 cut(s) 391, 580, 833, 955, 959
DpnII GATC 5 cut(s) 389, 578, 831, 953, 957
EaeI YGGCCR 3 cut(s) 127, 526, 924
Eam1104I CTCTTC 5 cut(s) 38, 112, 238, 665, 678
EarI CTCTTC 5 cut(s) 38, 112, 238, 665, 678
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 130, 927
Eco24I GRGCYC 2 cut(s) 109, 154
Eco32I GATATC 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 4 cut(s) 360, 414, 802, 897
EcoICRI GAGCTC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 874
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 130, 927
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 2 cut(s) 109, 154
ErhI CCWWGG 2 cut(s) 130, 927
FaeI CATG 1 cut(s) 134
FaqI GGGAC 1 cut(s) 900
FatI CATG 1 cut(s) 130
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 850
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 3 cut(s) 290, 388, 536
FriOI GRGCYC 2 cut(s) 109, 154
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 3 cut(s) 23, 111, 753
GluI GCNGC 1 cut(s) 262
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 2 cut(s) 41, 123
HaeIII GGCC 3 cut(s) 129, 528, 926
HapII CCGG 2 cut(s) 405, 529
Hin1II CATG 1 cut(s) 134
HincII GTYRAC 2 cut(s) 622, 909
HindII GTYRAC 2 cut(s) 622, 909
HinfI GANTC 2 cut(s) 48, 604
HpaII CCGG 2 cut(s) 405, 529
HphI GGTGA 4 cut(s) 41, 484, 555, 679
Hpy166II GTNNAC 4 cut(s) 63, 622, 637, 909
Hpy188I TCNGA 5 cut(s) 175, 379, 394, 459, 916
Hpy188III TCNNGA 4 cut(s) 155, 205, 576, 820
Hpy8I GTNNAC 4 cut(s) 63, 622, 637, 909
HpyAV CCTTC 5 cut(s) 84, 180, 582, 610, 658
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 4 cut(s) 274, 401, 452, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 900, 938
HpyF3I CTNAG 3 cut(s) 144, 265, 627
HpySE526I ACGT 1 cut(s) 199
Hsp92II CATG 1 cut(s) 134
Kzo9I GATC 5 cut(s) 389, 578, 831, 953, 957
LguI GCTCTTC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 104, 367
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 6 cut(s) 254, 268, 388, 410, 688, 928
MaeI CTAG 3 cut(s) 23, 111, 753
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 1 cut(s) 445
MalI GATC 5 cut(s) 391, 580, 833, 955, 959
MbiI CCGCTC 1 cut(s) 477
MboI GATC 5 cut(s) 389, 578, 831, 953, 957
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 4 cut(s) 109, 154, 364, 563
MlsI TGGCCA 2 cut(s) 129, 926
MluCI AATT 6 cut(s) 123, 162, 213, 344, 369, 732
MluNI TGGCCA 2 cut(s) 129, 926
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 7 cut(s) 3, 310, 496, 576, 579, 877, 975
Mox20I TGGCCA 2 cut(s) 129, 926
Mph1103I ATGCAT 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 2 cut(s) 129, 926
MseI TTAA 3 cut(s) 641, 945, 971
MslI CAYNNNNRTG 2 cut(s) 470, 965
Msp20I TGGCCA 2 cut(s) 129, 926
MspI CCGG 2 cut(s) 405, 529
MspR9I CCNGG 2 cut(s) 406, 876
Mva1269I GAATGC 1 cut(s) 141
MvaI CCWGG 1 cut(s) 876
MwoI GCNNNNNNNGC 3 cut(s) 149, 900, 938
NciI CCSGG 1 cut(s) 406
NcoI CCATGG 1 cut(s) 130
NdeI CATATG 1 cut(s) 850
NdeII GATC 5 cut(s) 389, 578, 831, 953, 957
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 3 cut(s) 106, 288, 798
NsiI ATGCAT 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 238
PctI GAATGC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 2 cut(s) 48, 604
PkrI GCNGC 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 200
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 3 cut(s) 106, 288, 798
PstNI CAGNNNCTG 1 cut(s) 825
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 62, 211
RsaNI GTAC 2 cut(s) 61, 210
RseI CAYNNNNRTG 2 cut(s) 470, 965
SacI GAGCTC 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 3 cut(s) 641, 945, 971
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 5 cut(s) 389, 578, 831, 953, 957
ScrFI CCNGG 2 cut(s) 406, 876
SduI GDGCHC 4 cut(s) 109, 154, 364, 563
SfaNI GCATC 6 cut(s) 254, 268, 388, 410, 688, 928
SfcI CTRYAG 2 cut(s) 303, 512
SmiMI CAYNNNNRTG 2 cut(s) 470, 965
Sse9I AATT 6 cut(s) 123, 162, 213, 344, 369, 732
SsiI CCGC 2 cut(s) 477, 545
SspMI CTAG 3 cut(s) 23, 111, 753
SstI GAGCTC 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 404, 874
StyI CCWWGG 2 cut(s) 130, 927
TaiI ACGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 204, 705, 956
TasI AATT 6 cut(s) 123, 162, 213, 344, 369, 732
TatI WGTACW 2 cut(s) 60, 209
TfiI GAWTC 2 cut(s) 48, 604
Tru1I TTAA 3 cut(s) 641, 945, 971
Tru9I TTAA 3 cut(s) 641, 945, 971
TscAI CASTG 1 cut(s) 606
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 2 cut(s) 450, 629
TspGWI ACGGA 2 cut(s) 112, 789
TspRI CASTG 1 cut(s) 606
XapI RAATTY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 3 cut(s) 23, 111, 753
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.