FvH4_2g00770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008074
Physical Location & Seq
Reverse (-)
778621 .. 781961
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00770.t1

Sequence Viewer

Length: 930 bp
ATGGAAGGAGGAGAAATTGGGGAGTTGCAGGACTGGGAGGTCCTCCACAGCGCCGGGGAGGACGTGGTGGAAAGCCATGGAAGCTTCTCGGCGATTGAGGCTGACGCCGAGGGGATGATCAGGTCTGATTATTTCTCGTTGGAGAATCAGGGGAGGTATGGCTATGCTAAGAGTGAAGCGGGTTCTGTTGAGTCGGATAATCCGAGCTGGATTGACCCGGTTTCGGTGACTACTTACAAGGAATCGGGTGGGTTCTGGTCCGATTCGGCTAGTGATCGGTCCGATGACCGAAAAAGTCATGATTTTGAGGCGAGGAGCGAGTTGGGTTCCGCCGGAAATGAGCAAAGCCCTGTGCTTTCGGAGCCTGTGGATGAGAATTTGGGGAAAGGCAAAGTGGGTGTCCAAGGAATTGGGGAAGTTGGGAGCTTTGATAAGGATTTGGATAAGTTCTGGTCTGATTCCGGCGGTGAAAGCTCGGTTTCTGTGAAATTTGACAAGGAGGGTGGTTCAAGTGAGTCCAACAAGGAATCAGGGGAGGAAGAATCAGCTGAGACTCAAAGTGGTGCAGTGGAAGAAGTGAAAACGGTGGCGAAACCGGGTGGTGAGGGTGAGAAGAGGATGGTCTTGTGGTGGAAGGTTCCATTGGAGGTGTTGAAGTATTGTGTTTTCAAGGTGAGTCCTGTTTGGTCTTTCTCTGTGGCCGCAGCGGTAATGGGTTTAGTTATCTTGGGAAGGAGATTGTATAGGATGAAGCGAAGGAGCCAGAGCTTCCAGCTCAAAGTTGCTCTGGATGACAAGAAGGTATCTCAATTCATGAGTCGCGCTGTGCGTCTCAATGAAGCATTTTCAGTTGTGAGGCGGGTTCCCATTTTACGACCTTCACTGCCGGCTGCTGGGGTCAATCCATGGCCAGTGATGAGTTTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

33.76

Weight (kDa)

4.88

Isoelectric Point (pI)

55.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 180 - 294 5.6e-14 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccII CGCG 1 cut(s) 822
AciI CCGC 6 cut(s) 179, 330, 465, 702, 707, 859
AcoI YGGCCR 2 cut(s) 699, 908
AcsI RAATTY 2 cut(s) 376, 488
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 223, 893
AgsI TTSAA 3 cut(s) 510, 655, 670
AjiI CACGTC 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AluBI AGCT 7 cut(s) 84, 207, 426, 474, 548, 768, 775
AluI AGCT 7 cut(s) 84, 207, 426, 474, 548, 768, 775
Alw26I GTCTC 2 cut(s) 545, 836
AoxI GGCC 2 cut(s) 699, 908
ApeKI GCWGC 2 cut(s) 704, 890
ApoI RAATTY 2 cut(s) 376, 488
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspLEI GCGC 2 cut(s) 53, 824
AspS9I GGNCC 3 cut(s) 40, 258, 279
AsuC2I CCSGG 3 cut(s) 55, 218, 597
AsuHPI GGTGA 5 cut(s) 238, 479, 614, 620, 685
AvaII GGWCC 3 cut(s) 40, 258, 279
BalI TGGCCA 1 cut(s) 910
BbvI GCAGC 2 cut(s) 716, 877
BccI CCATC 1 cut(s) 613
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 3 cut(s) 55, 218, 597
BcoDI GTCTC 2 cut(s) 545, 836
BfaI CTAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 3 cut(s) 702, 705, 891
BlsI GCNGC 3 cut(s) 703, 706, 892
Bme1390I CCNGG 3 cut(s) 55, 218, 597
Bme18I GGWCC 3 cut(s) 40, 258, 279
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 3 cut(s) 40, 258, 279
BmiI GGNNCC 5 cut(s) 328, 363, 639, 761, 864
BmrFI CCNGG 3 cut(s) 55, 218, 597
BmrI ACTGGG 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 43
BpuMI CCSGG 3 cut(s) 55, 218, 597
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 5 cut(s) 54, 76, 108, 403, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 893
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 2 cut(s) 38, 911
BseDI CCNNGG 5 cut(s) 54, 76, 108, 403, 905
BseGI GGATG 5 cut(s) 120, 376, 624, 753, 796
BseLI CCNNNNNNNGG 2 cut(s) 223, 893
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 38, 911
BseRI GAGGAG 2 cut(s) 24, 328
BseXI GCAGC 2 cut(s) 716, 877
BseYI CCCAGC 1 cut(s) 893
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 822
BshFI GGCC 2 cut(s) 701, 910
BsiSI CCGG 6 cut(s) 54, 218, 333, 462, 596, 887
BslI CCNNNNNNNGG 2 cut(s) 223, 893
BsmAI GTCTC 2 cut(s) 545, 836
BsmBI CGTCTC 1 cut(s) 836
BsnI GGCC 2 cut(s) 701, 910
Bsp143I GATC 2 cut(s) 117, 274
Bsp19I CCATGG 2 cut(s) 76, 905
BspACI CCGC 6 cut(s) 179, 330, 465, 702, 707, 859
BspANI GGCC 2 cut(s) 701, 910
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 1 cut(s) 822
BspHI TCATGA 2 cut(s) 298, 813
BspLI GGNNCC 5 cut(s) 328, 363, 639, 761, 864
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 2 cut(s) 38, 911
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 5 cut(s) 54, 76, 108, 403, 905
BssMI GATC 2 cut(s) 117, 274
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 3 cut(s) 76, 403, 905
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 608
BstACI GRCGYC 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 2 cut(s) 168, 549
BstDSI CCRYGG 2 cut(s) 76, 905
BstF5I GGATG 5 cut(s) 120, 376, 624, 753, 796
BstFNI CGCG 1 cut(s) 822
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 824
BstKTI GATC 2 cut(s) 120, 277
BstMAI GTCTC 2 cut(s) 545, 836
BstMBI GATC 2 cut(s) 117, 274
BstMWI GCNNNNNNNGC 4 cut(s) 81, 98, 361, 471
BstSCI CCNGG 3 cut(s) 53, 216, 595
BstUI CGCG 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 716, 877
BstXI CCANNNNNNTGG 1 cut(s) 410
BsuRI GGCC 2 cut(s) 701, 910
BtgI CCRYGG 2 cut(s) 76, 905
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 5 cut(s) 120, 376, 624, 753, 796
BtsI GCAGTG 2 cut(s) 573, 881
BtsIMutI CAGTG 3 cut(s) 573, 881, 918
Cac8I GCNNGC 1 cut(s) 888
CciI TCATGA 2 cut(s) 298, 813
CfoI GCGC 2 cut(s) 53, 824
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 3 cut(s) 40, 258, 279
CpoI CGGWCCG 1 cut(s) 279
CseI GACGC 2 cut(s) 113, 818
CspCI CAANNNNNGTGG 2 cut(s) 484, 519
CspI CGGWCCG 1 cut(s) 279
CviAII CATG 4 cut(s) 77, 299, 814, 906
DdeI CTNAG 2 cut(s) 168, 549
DpnI GATC 2 cut(s) 119, 276
DpnII GATC 2 cut(s) 117, 274
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 699, 908
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
EciI GGCGGA 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 76, 403, 905
Eco47I GGWCC 3 cut(s) 40, 258, 279
EcoO109I RGGNCCY 1 cut(s) 40
EcoT14I CCWWGG 3 cut(s) 76, 403, 905
ErhI CCWWGG 3 cut(s) 76, 403, 905
Esp3I CGTCTC 1 cut(s) 836
FaeI CATG 4 cut(s) 80, 302, 817, 909
FaiI YATR 7 cut(s) 78, 159, 165, 300, 744, 815, 907
FatI CATG 4 cut(s) 76, 298, 813, 905
FauI CCCGC 2 cut(s) 172, 852
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 702, 705, 891
FokI GGATG 5 cut(s) 127, 383, 631, 760, 803
Fsp4HI GCNGC 3 cut(s) 702, 705, 891
FspBI CTAG 1 cut(s) 270
GlaI GCGC 2 cut(s) 52, 823
GluI GCNGC 3 cut(s) 702, 705, 891
GsaI CCCAGC 1 cut(s) 897
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 2 cut(s) 701, 910
HapII CCGG 6 cut(s) 54, 218, 333, 462, 596, 887
HgaI GACGC 2 cut(s) 113, 818
HhaI GCGC 2 cut(s) 53, 824
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 4 cut(s) 80, 302, 817, 909
Hin6I GCGC 2 cut(s) 51, 822
HinP1I GCGC 2 cut(s) 51, 822
HindIII AAGCTT 1 cut(s) 82
HpaII CCGG 6 cut(s) 54, 218, 333, 462, 596, 887
HphI GGTGA 5 cut(s) 238, 479, 614, 620, 685
Hpy188I TCNGA 7 cut(s) 127, 196, 204, 262, 283, 361, 457
Hpy188III TCNNGA 3 cut(s) 299, 788, 814
HpyAV CCTTC 5 cut(s) 628, 726, 750, 793, 888
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 28, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 98, 361, 471
HpyF3I CTNAG 2 cut(s) 168, 549
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 4 cut(s) 80, 302, 817, 909
HspAI GCGC 2 cut(s) 51, 822
KroI GCCGGC 1 cut(s) 886
KroNI GCCGGC 1 cut(s) 888
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 2 cut(s) 117, 274
LmnI GCTCC 4 cut(s) 315, 361, 423, 759
Lsp1109I GCAGC 2 cut(s) 716, 877
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 119, 276
MboI GATC 2 cut(s) 117, 274
MboII GAAGA 3 cut(s) 551, 584, 625
MlsI TGGCCA 1 cut(s) 910
MluCI AATT 5 cut(s) 15, 376, 408, 488, 809
MluNI TGGCCA 1 cut(s) 910
MlyI GAGTC 5 cut(s) 200, 524, 547, 685, 826
MmeI TCCRAC 3 cut(s) 120, 174, 543
Mox20I TGGCCA 1 cut(s) 910
MroNI GCCGGC 1 cut(s) 886
MscI TGGCCA 1 cut(s) 910
Msp20I TGGCCA 1 cut(s) 910
MspA1I CMGCKG 2 cut(s) 548, 707
MspI CCGG 6 cut(s) 54, 218, 333, 462, 596, 887
MspR9I CCNGG 3 cut(s) 55, 218, 597
MvnI CGCG 1 cut(s) 822
MwoI GCNNNNNNNGC 4 cut(s) 81, 98, 361, 471
NaeI GCCGGC 1 cut(s) 888
NciI CCSGG 3 cut(s) 55, 218, 597
NcoI CCATGG 2 cut(s) 76, 905
NdeII GATC 2 cut(s) 117, 274
NgoMIV GCCGGC 1 cut(s) 886
NlaIII CATG 4 cut(s) 80, 302, 817, 909
NlaIV GGNNCC 5 cut(s) 328, 363, 639, 761, 864
NmeAIII GCCGAG 2 cut(s) 68, 133
NmuCI GTSAC 1 cut(s) 226
PagI TCATGA 2 cut(s) 298, 813
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 826
PdiI GCCGGC 1 cut(s) 888
PfeI GAWTC 6 cut(s) 145, 242, 263, 458, 527, 542
PkrI GCNGC 3 cut(s) 703, 706, 892
PleI GAGTC 5 cut(s) 199, 523, 547, 684, 825
PpsI GAGTC 5 cut(s) 199, 523, 547, 684, 825
PpuMI RGGWCCY 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 893
PspN4I GGNNCC 5 cut(s) 328, 363, 639, 761, 864
PspPI GGNCC 3 cut(s) 40, 258, 279
PspPPI RGGWCCY 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 548
Rsr2I CGGWCCG 1 cut(s) 279
RsrII CGGWCCG 1 cut(s) 279
SatI GCNGC 3 cut(s) 702, 705, 891
Sau3AI GATC 2 cut(s) 117, 274
Sau96I GGNCC 3 cut(s) 40, 258, 279
SchI GAGTC 5 cut(s) 200, 524, 547, 685, 826
ScrFI CCNGG 3 cut(s) 55, 218, 597
SinI GGWCC 3 cut(s) 40, 258, 279
Sse9I AATT 5 cut(s) 15, 376, 408, 488, 809
SsiI CCGC 6 cut(s) 179, 330, 465, 702, 707, 859
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 53, 216, 595
StyI CCWWGG 3 cut(s) 76, 403, 905
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 66
TaqII GACCGA 2 cut(s) 267, 303
TasI AATT 5 cut(s) 15, 376, 408, 488, 809
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 6 cut(s) 145, 242, 263, 458, 527, 542
TscAI CASTG 3 cut(s) 573, 888, 918
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 704, 890
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 764, 802, 852
TspRI CASTG 3 cut(s) 573, 888, 918
VpaK11BI GGWCC 3 cut(s) 40, 258, 279
XapI RAATTY 2 cut(s) 376, 488
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.