pycom10g00350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008074
Physical Location & Seq
Reverse (-)
416198 .. 418738
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00350.1

Sequence Viewer

Length: 1038 bp
ATGGACGGAGGACAAGGAGAAGTTGGTGAGTTGCAGGACTGGGAGGTGCTAAACAGCTCGGACCCTACCCTGGTTAACTCAGACGACTCGATTGATAGCCCTAGGAGCTTCGAGGGAATCGATGTGCACGCCGAAGGTATGATCAGGCCTGATTACTTCTCCCTGGATAATCATGGGAAGTTGGGGTATGGTAAGGCGGTGATTGATGTCAGCGAAGACGGTTCGGTTGAGTCCGACAACCCCAGTTGGATTGACCCGGGTTCCGTGACCCAATATGGTGGAAAAGAATCGGGTGGGTTTTGGCCCGATTCGGCAAGTGATCGGTCCGATGACCGAAAAAGTAATGATTTTGAGGGGAAAAAAGAATTGGGTTTTGTGGGAAGTGAGAAAGGCCATGTGGGTTGTGGAAGGGCTGGAGAAATGGAAGCTGTGGATGAGAAAGTTAGTGGGTTGGATGCTCAGGGCCGAGTGAGCTTTGAAGAAATTGGGGAAAACGGAAGCCTTGATAAGGATTTGGGTAAGTTCTGGTCTGATTCAGGCGGCGACAGTCTGGTTTCTTTGAAATATGAGAAGGTTGCGATGGAAAGCGAGATAGGTTTTAGTGGCTTGGAATGTGCAATTAACTTGGATAATGAGCCAGAAGAAGGACAATCGGTTGAGAGTCAAAGTAGTGCAGTTGAAGAGGAGAAATCAGAGGCGAAATCCGGTGAAGAGGTAGTGAAGAGGACAATCCTGTGGTGGAAGGTCCCATTTGAGGTCTTGAAGTATTGTGTTTTCAGGGTAAGCCCTGTTTGGTCCTTGTCTGTGGCTGCAGCGGTAATGGGTTTAGTGATCTTGGGGCGGAGATTGCACAAGATGAAGCGAAAGAGCCAAAGCTTGCAGCTCAAGGTTACTCTGGATGATAAGAAGGTGTCTCAGTTCATGAGCCGTGCTGTGCGTCTCAATGAAGCATTTTCAGTGGTGAAGCGAGTTCCTATAATGCGACCTTCGCTGCCAGCAGCTGGAATAAATCCATGGCCTGTAATGGGTTTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

346

Amino Acids

37.66

Weight (kDa)

4.73

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 207 - 330 1.2e-14 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1001
AciI CCGC 4 cut(s) 197, 540, 815, 841
AfiI CCNNNNNNNGG 6 cut(s) 70, 508, 644, 754, 1001, 1025
AgsI TTSAA 4 cut(s) 479, 562, 680, 763
AjnI CCWGG 2 cut(s) 69, 162
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 7 cut(s) 57, 108, 428, 474, 876, 883, 1001
AluI AGCT 7 cut(s) 57, 108, 428, 474, 876, 883, 1001
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 918, 944
Alw44I GTGCAC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 1001
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 5 cut(s) 146, 302, 391, 463, 1016
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 5 cut(s) 809, 812, 880, 991, 998
AspA2I CCTAGG 1 cut(s) 101
AspS9I GGNCC 6 cut(s) 61, 303, 324, 463, 745, 795
AsuC2I CCSGG 2 cut(s) 257, 258
AsuHPI GGTGA 4 cut(s) 38, 211, 719, 973
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 4 cut(s) 61, 324, 745, 795
AvrII CCTAGG 1 cut(s) 101
BaeGI GKGCMC 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 796, 824, 892, 978, 1010
BccI CCATC 1 cut(s) 574
BceAI ACGGC 1 cut(s) 912
BciT130I CCWGG 2 cut(s) 71, 164
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 2 cut(s) 257, 258
BcoDI GTCTC 2 cut(s) 918, 944
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 810
BisI GCNGC 6 cut(s) 541, 810, 813, 881, 992, 999
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 6 cut(s) 542, 811, 814, 882, 993, 1000
Bme1390I CCNGG 4 cut(s) 71, 164, 257, 258
Bme18I GGWCC 4 cut(s) 61, 324, 745, 795
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 61, 303, 324, 463, 745, 795
BmiI GGNNCC 3 cut(s) 63, 262, 747
BmrFI CCNGG 4 cut(s) 71, 164, 257, 258
BmrI ACTGGG 2 cut(s) 49, 237
BmsI GCATC 1 cut(s) 445
BmuI ACTGGG 2 cut(s) 49, 237
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 869
BpuMI CCSGG 2 cut(s) 257, 258
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 5 cut(s) 69, 101, 162, 256, 1013
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 508, 644, 754, 1001, 1025
Bse1I ACTGG 2 cut(s) 44, 243
BseBI CCWGG 2 cut(s) 71, 164
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 5 cut(s) 69, 101, 162, 256, 1013
BseGI GGATG 3 cut(s) 439, 460, 904
BseLI CCNNNNNNNGG 6 cut(s) 70, 508, 644, 754, 1001, 1025
BseMII CTCAG 3 cut(s) 93, 473, 929
BseNI ACTGG 2 cut(s) 44, 243
BseRI GAGGAG 1 cut(s) 698
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 5 cut(s) 796, 824, 892, 978, 1010
BsgI GTGCAG 1 cut(s) 693
BshFI GGCC 5 cut(s) 148, 304, 393, 465, 1018
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 2 cut(s) 257, 705
BslFI GGGAC 1 cut(s) 731
BslI CCNNNNNNNGG 6 cut(s) 70, 508, 644, 754, 1001, 1025
BsmAI GTCTC 2 cut(s) 918, 944
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 1 cut(s) 731
BsnI GGCC 5 cut(s) 148, 304, 393, 465, 1018
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 141, 319, 831
Bsp19I CCATGG 1 cut(s) 1013
BspACI CCGC 4 cut(s) 197, 540, 815, 841
BspANI GGCC 5 cut(s) 148, 304, 393, 465, 1018
BspCNI CTCAG 3 cut(s) 92, 472, 928
BspDI ATCGAT 1 cut(s) 120
BspHI TCATGA 1 cut(s) 921
BspLI GGNNCC 3 cut(s) 63, 262, 747
BspMAI CTGCAG 1 cut(s) 814
BsrI ACTGG 2 cut(s) 44, 243
BssECI CCNNGG 5 cut(s) 69, 101, 162, 256, 1013
BssMI GATC 3 cut(s) 141, 319, 831
BssT1I CCWWGG 2 cut(s) 101, 1013
Bst2UI CCWGG 2 cut(s) 71, 164
Bst4CI ACNGT 2 cut(s) 221, 548
Bst6I CTCTTC 3 cut(s) 675, 705, 716
BstC8I GCNNGC 3 cut(s) 129, 878, 996
BstDEI CTNAG 3 cut(s) 79, 459, 915
BstDSI CCRYGG 1 cut(s) 1013
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 3 cut(s) 439, 460, 904
BstKTI GATC 3 cut(s) 144, 322, 834
BstMAI GTCTC 2 cut(s) 918, 944
BstMBI GATC 3 cut(s) 141, 319, 831
BstMWI GCNNNNNNNGC 4 cut(s) 105, 471, 847, 988
BstNI CCWGG 2 cut(s) 71, 164
BstSCI CCNGG 4 cut(s) 69, 162, 255, 256
BstSFI CTRYAG 1 cut(s) 810
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 5 cut(s) 796, 824, 892, 978, 1010
BstV2I GAAGAC 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 5 cut(s) 148, 304, 393, 465, 1018
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 1013
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 3 cut(s) 439, 460, 904
BtsIMutI CAGTG 1 cut(s) 963
Cac8I GCNNGC 3 cut(s) 129, 878, 996
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 1 cut(s) 921
Cfr13I GGNCC 6 cut(s) 61, 303, 324, 463, 745, 795
Cfr9I CCCGGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 324
CseI GACGC 1 cut(s) 926
CspI CGGWCCG 1 cut(s) 324
CviAII CATG 4 cut(s) 173, 395, 922, 1014
DdeI CTNAG 3 cut(s) 79, 459, 915
DpnI GATC 3 cut(s) 143, 321, 833
DpnII GATC 3 cut(s) 141, 319, 831
Eam1104I CTCTTC 3 cut(s) 675, 705, 716
EarI CTCTTC 3 cut(s) 675, 705, 716
EciI GGCGGA 1 cut(s) 856
Eco130I CCWWGG 2 cut(s) 101, 1013
Eco147I AGGCCT 1 cut(s) 148
Eco47I GGWCC 4 cut(s) 61, 324, 745, 795
Eco88I CYCGRG 1 cut(s) 256
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 745
EcoRII CCWGG 2 cut(s) 69, 162
EcoT14I CCWWGG 2 cut(s) 101, 1013
ErhI CCWWGG 2 cut(s) 101, 1013
Esp3I CGTCTC 1 cut(s) 944
FaeI CATG 4 cut(s) 176, 398, 925, 1017
FaiI YATR 9 cut(s) 140, 174, 189, 276, 396, 567, 923, 977, 1015
FaqI GGGAC 1 cut(s) 731
FatI CATG 4 cut(s) 172, 394, 921, 1013
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 541, 810, 813, 881, 992, 999
FokI GGATG 3 cut(s) 446, 467, 911
Fsp4HI GCNGC 6 cut(s) 541, 810, 813, 881, 992, 999
FspBI CTAG 1 cut(s) 102
GluI GCNGC 6 cut(s) 541, 810, 813, 881, 992, 999
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 5 cut(s) 148, 304, 393, 465, 1018
HapII CCGG 2 cut(s) 257, 705
HgaI GACGC 1 cut(s) 926
Hin1II CATG 4 cut(s) 176, 398, 925, 1017
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 7 cut(s) 86, 117, 230, 287, 308, 533, 661
HpaI GTTAAC 1 cut(s) 76
HpaII CCGG 2 cut(s) 257, 705
HphI GGTGA 4 cut(s) 38, 211, 719, 973
Hpy166II GTNNAC 2 cut(s) 76, 127
Hpy188I TCNGA 6 cut(s) 61, 82, 235, 328, 532, 694
Hpy188III TCNNGA 3 cut(s) 760, 896, 922
Hpy8I GTNNAC 2 cut(s) 76, 127
HpyAV CCTTC 7 cut(s) 128, 402, 565, 638, 736, 901, 996
HpyCH4III ACNGT 2 cut(s) 221, 548
HpyCH4V TGCA 7 cut(s) 34, 127, 617, 674, 812, 850, 880
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 471, 847, 988
HpyF3I CTNAG 3 cut(s) 79, 459, 915
Hsp92II CATG 4 cut(s) 176, 398, 925, 1017
Ksp22I TGATCA 1 cut(s) 141
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 141, 319, 831
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 5 cut(s) 796, 824, 892, 978, 1010
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 265, 889
MalI GATC 3 cut(s) 143, 321, 833
MboI GATC 3 cut(s) 141, 319, 831
MboII GAAGA 6 cut(s) 227, 491, 653, 692, 722, 733
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 3 cut(s) 365, 483, 618
MlyI GAGTC 3 cut(s) 80, 239, 670
MmeI TCCRAC 3 cut(s) 227, 258, 432
MnlI CCTC 8 cut(s) 37, 106, 346, 676, 688, 706, 717, 748
MseI TTAA 2 cut(s) 75, 621
MspA1I CMGCKG 2 cut(s) 815, 1001
MspI CCGG 2 cut(s) 257, 705
MspR9I CCNGG 4 cut(s) 71, 164, 257, 258
MvaI CCWGG 2 cut(s) 71, 164
MwoI GCNNNNNNNGC 4 cut(s) 105, 471, 847, 988
NciI CCSGG 2 cut(s) 257, 258
NcoI CCATGG 1 cut(s) 1013
NdeII GATC 3 cut(s) 141, 319, 831
NlaIII CATG 4 cut(s) 176, 398, 925, 1017
NlaIV GGNNCC 3 cut(s) 63, 262, 747
NmeAIII GCCGAG 1 cut(s) 491
NmuCI GTSAC 1 cut(s) 265
PagI TCATGA 1 cut(s) 921
PceI AGGCCT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 4 cut(s) 117, 287, 308, 533
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 6 cut(s) 542, 811, 814, 882, 993, 1000
PleI GAGTC 3 cut(s) 80, 238, 669
PpsI GAGTC 3 cut(s) 80, 238, 669
PpuMI RGGWCCY 1 cut(s) 745
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 2 cut(s) 69, 162
PspGI CCWGG 2 cut(s) 69, 162
PspN4I GGNNCC 3 cut(s) 63, 262, 747
PspPI GGNCC 6 cut(s) 61, 303, 324, 463, 745, 795
PspPPI RGGWCCY 1 cut(s) 745
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 1001
PvuII CAGCTG 1 cut(s) 1001
Rsr2I CGGWCCG 1 cut(s) 324
RsrII CGGWCCG 1 cut(s) 324
SaqAI TTAA 2 cut(s) 75, 621
SatI GCNGC 6 cut(s) 541, 810, 813, 881, 992, 999
Sau3AI GATC 3 cut(s) 141, 319, 831
Sau96I GGNCC 6 cut(s) 61, 303, 324, 463, 745, 795
SchI GAGTC 3 cut(s) 80, 239, 670
ScrFI CCNGG 4 cut(s) 71, 164, 257, 258
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 810
SinI GGWCC 4 cut(s) 61, 324, 745, 795
SmaI CCCGGG 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 3 cut(s) 365, 483, 618
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 4 cut(s) 197, 540, 815, 841
SspMI CTAG 1 cut(s) 102
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 4 cut(s) 69, 162, 255, 256
StyI CCWWGG 2 cut(s) 101, 1013
TaaI ACNGT 2 cut(s) 221, 548
TaqI TCGA 3 cut(s) 89, 111, 120
TaqII GACCGA 2 cut(s) 312, 348
TasI AATT 3 cut(s) 365, 483, 618
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 4 cut(s) 117, 287, 308, 533
Tru1I TTAA 2 cut(s) 75, 621
Tru9I TTAA 2 cut(s) 75, 621
TscAI CASTG 1 cut(s) 963
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 5 cut(s) 809, 812, 880, 991, 998
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 872, 910, 960
TspGWI ACGGA 3 cut(s) 21, 253, 510
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 1 cut(s) 963
Van91I CCANNNNNTGG 1 cut(s) 1001
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 4 cut(s) 61, 324, 745, 795
XagI CCTNNNNNAGG 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 256
XmaJI CCTAGG 1 cut(s) 101
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.