MD05G1003400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008074
Physical Location & Seq
Reverse (-)
1108786 .. 1112421
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1003400.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGAAGGAGGAGGAGAAGTTGGTGAGTTGCAGGACTGGGAGGTGCTCCACAGCTTCGACCCTGCCCTGGTTAACTCGGTCGACTCGGTTCAAACCCCCAGGAGCTTCGAGGGAATCGATGTTCACGCCGAGGGTATGATCAGGCCTGATTACTTCTCTCTCGATAACCATGACAAATTTGGGTATGGTAAGGCGGTGGTTGATGTCGCCGAAGAGGGTTCTGTTGAGTCCGACAACCCCAGTTGGATTGACCCGGATTCGGTGACTCGCTATGGCGGAAAAGAATCGGGTGGGTTTTGGTCCGATTCGGCAAGTGACCGGTCCGATGACCGTAAAAGTAATGATTTTGAGGGCAAAAACGAATTGGGTTTTGTGGAAAATGAGAAAAGCCATGTGGGTTTTGAGGGGTTTGGAGAAATGGAAGCTGTGGACGAGAATTTTGGGAAATTTGGGTTGGATGCTCAGGGCCGAGTGAGCTTTGAAGAAATTGGGGAAAACGGAAGCCTTGATAAGGATTTGGGTAAGTTATGGTCTGATTCAGGCGGCGACAGTCTGGTTTCGGCGAAATTTGAGAAGGTTGCGAAGGATAGCGAGATAGGTTTTAGTGGTTTGGAAAGTGGAAATAACTTGGACAATGAACCAGAAGAAGGACAATCAGTTGAGAGTCAAAGTGCTGCAGTTGAAGAAGAGAAATCGGAGGCGAAATCCGGTGAAGAGGTAGTGAAGAGGACAATCCTGTGGTGGAAGGTCCCATTTGAGGTTTTAAAGTATTGTGTTTTCAGGGTAAGCCCTGTTTGGTCCTTGTCCGTCGCTGCAGCTGTAATGGGTTTGGTGTTCTTGGGGCGGAGATTGCGCAAGATGAAGCGGAAGAGCCAGAGCCTGCAGCTCAAGGTTACTCTGGATGATAAGAAGGTGTCTCAGTTCACGAGTCGTGCTGCACGTCTCAATGAAGCATTTTCAGTGGTGAGGCGGGTGCCTACAGTGCGACCTTCGCTGCCAGCAGCCGGAGTAAATCCATGGCCTGTAATGGGTTTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

347

Amino Acids

38.05

Weight (kDa)

4.72

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 211 - 331 9.9e-15 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 973
AccI GTMKAC 1 cut(s) 81
AciI CCGC 6 cut(s) 194, 276, 543, 844, 865, 970
AcsI RAATTY 4 cut(s) 176, 436, 446, 566
AfiI CCNNNNNNNGG 7 cut(s) 67, 259, 511, 647, 757, 1004, 1028
AgeI ACCGGT 1 cut(s) 318
AgsI TTSAA 3 cut(s) 92, 482, 683
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 2 cut(s) 66, 98
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 6 cut(s) 54, 105, 425, 477, 818, 886
AluI AGCT 6 cut(s) 54, 105, 425, 477, 818, 886
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 2 cut(s) 921, 947
AlwNI CAGNNNCTG 1 cut(s) 880
AoxI GGCC 3 cut(s) 143, 466, 1019
ApeKI GCWGC 7 cut(s) 674, 812, 815, 883, 935, 994, 1001
ApoI RAATTY 4 cut(s) 176, 436, 446, 566
ArsI GACNNNNNNTTYG 2 cut(s) 422, 454
AsiGI ACCGGT 1 cut(s) 318
AspLEI GCGC 1 cut(s) 855
AspS9I GGNCC 5 cut(s) 300, 321, 466, 748, 798
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 4 cut(s) 35, 274, 722, 976
AvaII GGWCC 4 cut(s) 300, 321, 748, 798
BanI GGYRCC 1 cut(s) 973
BauI CACGAG 1 cut(s) 925
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 7 cut(s) 661, 799, 827, 895, 922, 981, 1013
BciT130I CCWGG 2 cut(s) 68, 100
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 921, 947
BfmI CTRYAG 4 cut(s) 675, 813, 881, 978
BisI GCNGC 8 cut(s) 544, 675, 813, 816, 884, 936, 995, 1002
BlsI GCNGC 8 cut(s) 545, 676, 814, 817, 885, 937, 996, 1003
Bme1390I CCNGG 3 cut(s) 68, 100, 254
Bme18I GGWCC 4 cut(s) 300, 321, 748, 798
BmgBI CACGTC 1 cut(s) 941
BmgT120I GGNCC 5 cut(s) 300, 321, 466, 748, 798
BmiI GGNNCC 2 cut(s) 750, 975
BmrFI CCNGG 3 cut(s) 68, 100, 254
BmrI ACTGGG 2 cut(s) 46, 234
BmsI GCATC 1 cut(s) 448
BmuI ACTGGG 2 cut(s) 46, 234
Bpu10I CCTNAGC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 872
BpuMI CCSGG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 117
BsaJI CCNNGG 4 cut(s) 66, 98, 129, 1016
BsaWI WCCGGW 2 cut(s) 318, 707
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 7 cut(s) 67, 259, 511, 647, 757, 1004, 1028
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 2 cut(s) 41, 240
BseBI CCWGG 2 cut(s) 68, 100
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 4 cut(s) 66, 98, 129, 1016
BseGI GGATG 2 cut(s) 463, 907
BseLI CCNNNNNNNGG 7 cut(s) 67, 259, 511, 647, 757, 1004, 1028
BseMII CTCAG 2 cut(s) 476, 932
BseNI ACTGG 2 cut(s) 41, 240
BseRI GAGGAG 2 cut(s) 24, 27
BseXI GCAGC 7 cut(s) 661, 799, 827, 895, 922, 981, 1013
BsgI GTGCAG 1 cut(s) 921
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 3 cut(s) 145, 468, 1021
BshNI GGYRCC 1 cut(s) 973
BshTI ACCGGT 1 cut(s) 318
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 48
BsiSI CCGG 4 cut(s) 254, 319, 708, 1005
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 7 cut(s) 67, 259, 511, 647, 757, 1004, 1028
BsmAI GTCTC 2 cut(s) 921, 947
BsmBI CGTCTC 1 cut(s) 947
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 3 cut(s) 145, 468, 1021
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 1016
BspACI CCGC 6 cut(s) 194, 276, 543, 844, 865, 970
BspANI GGCC 3 cut(s) 145, 468, 1021
BspCNI CTCAG 2 cut(s) 475, 931
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 750, 975
BspMAI CTGCAG 3 cut(s) 679, 817, 885
BspQI GCTCTTC 1 cut(s) 863
BspT107I GGYRCC 1 cut(s) 973
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 2 cut(s) 41, 240
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 4 cut(s) 66, 98, 129, 1016
BssMI GATC 1 cut(s) 138
BssSI CACGAG 1 cut(s) 925
BssT1I CCWWGG 1 cut(s) 1016
Bst2BI CACGAG 1 cut(s) 925
Bst2UI CCWGG 2 cut(s) 68, 100
Bst4CI ACNGT 3 cut(s) 332, 551, 982
Bst6I CTCTTC 5 cut(s) 207, 681, 708, 719, 863
BstC8I GCNNGC 2 cut(s) 881, 999
BstDEI CTNAG 2 cut(s) 462, 918
BstDSI CCRYGG 1 cut(s) 1016
BstENI CCTNNNNNAGG 1 cut(s) 509
BstF5I GGATG 2 cut(s) 463, 907
BstHHI GCGC 1 cut(s) 855
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 2 cut(s) 921, 947
BstMBI GATC 1 cut(s) 138
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 4 cut(s) 474, 850, 982, 991
BstNI CCWGG 2 cut(s) 68, 100
BstSCI CCNGG 3 cut(s) 66, 98, 252
BstSFI CTRYAG 4 cut(s) 675, 813, 881, 978
BstV1I GCAGC 7 cut(s) 661, 799, 827, 895, 922, 981, 1013
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 3 cut(s) 145, 468, 1021
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 1016
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 2 cut(s) 463, 907
BtsIMutI CAGTG 2 cut(s) 966, 987
Cac8I GCNNGC 2 cut(s) 881, 999
CaiI CAGNNNCTG 1 cut(s) 880
CfoI GCGC 1 cut(s) 855
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 5 cut(s) 300, 321, 466, 748, 798
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 321
CspAI ACCGGT 1 cut(s) 318
CspI CGGWCCG 1 cut(s) 321
CviAII CATG 3 cut(s) 170, 392, 1017
DdeI CTNAG 2 cut(s) 462, 918
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
DraI TTTAAA 1 cut(s) 765
Eam1104I CTCTTC 5 cut(s) 207, 681, 708, 719, 863
EarI CTCTTC 5 cut(s) 207, 681, 708, 719, 863
EciI GGCGGA 2 cut(s) 291, 859
Eco130I CCWWGG 1 cut(s) 1016
Eco147I AGGCCT 1 cut(s) 145
Eco47I GGWCC 4 cut(s) 300, 321, 748, 798
EcoNI CCTNNNNNAGG 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 748
EcoRII CCWGG 2 cut(s) 66, 98
EcoT14I CCWWGG 1 cut(s) 1016
ErhI CCWWGG 1 cut(s) 1016
Esp3I CGTCTC 1 cut(s) 947
FaeI CATG 3 cut(s) 173, 395, 1020
FaiI YATR 7 cut(s) 137, 171, 186, 273, 393, 529, 1018
FaqI GGGAC 1 cut(s) 734
FatI CATG 3 cut(s) 169, 391, 1016
FauI CCCGC 1 cut(s) 963
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 8 cut(s) 544, 675, 813, 816, 884, 936, 995, 1002
FokI GGATG 2 cut(s) 470, 914
Fsp4HI GCNGC 8 cut(s) 544, 675, 813, 816, 884, 936, 995, 1002
FspI TGCGCA 1 cut(s) 854
GlaI GCGC 1 cut(s) 854
GluI GCNGC 8 cut(s) 544, 675, 813, 816, 884, 936, 995, 1002
HaeIII GGCC 3 cut(s) 145, 468, 1021
HapII CCGG 4 cut(s) 254, 319, 708, 1005
HhaI GCGC 1 cut(s) 855
Hin1II CATG 3 cut(s) 173, 395, 1020
Hin6I GCGC 1 cut(s) 853
HinP1I GCGC 1 cut(s) 853
HincII GTYRAC 2 cut(s) 73, 82
HindII GTYRAC 2 cut(s) 73, 82
HpaI GTTAAC 1 cut(s) 73
HpaII CCGG 4 cut(s) 254, 319, 708, 1005
HphI GGTGA 4 cut(s) 35, 274, 722, 976
Hpy166II GTNNAC 5 cut(s) 73, 82, 124, 430, 924
Hpy188I TCNGA 5 cut(s) 232, 304, 325, 535, 697
Hpy188III TCNNGA 3 cut(s) 161, 899, 925
Hpy8I GTNNAC 5 cut(s) 73, 82, 124, 430, 924
Hpy99I CGWCG 1 cut(s) 812
HpyAV CCTTC 6 cut(s) 568, 577, 641, 739, 904, 999
HpyCH4III ACNGT 3 cut(s) 332, 551, 982
HpyCH4IV ACGT 1 cut(s) 940
HpyCH4V TGCA 5 cut(s) 31, 677, 815, 883, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 474, 850, 982, 991
HpyF3I CTNAG 2 cut(s) 462, 918
HpySE526I ACGT 1 cut(s) 940
Hsp92II CATG 3 cut(s) 173, 395, 1020
HspAI GCGC 1 cut(s) 853
Ksp22I TGATCA 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 138
LguI GCTCTTC 1 cut(s) 863
LmnI GCTCC 2 cut(s) 51, 102
Lsp1109I GCAGC 7 cut(s) 661, 799, 827, 895, 922, 981, 1013
LweI GCATC 1 cut(s) 448
MaeII ACGT 1 cut(s) 940
MaeIII GTNAC 3 cut(s) 262, 314, 892
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 8 cut(s) 224, 494, 656, 695, 698, 725, 736, 880
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 6 cut(s) 176, 362, 436, 446, 486, 566
MlyI GAGTC 5 cut(s) 77, 236, 259, 673, 937
MmeI TCCRAC 3 cut(s) 224, 255, 435
MseI TTAA 2 cut(s) 72, 764
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 4 cut(s) 254, 319, 708, 1005
MspR9I CCNGG 3 cut(s) 68, 100, 254
MvaI CCWGG 2 cut(s) 68, 100
MwoI GCNNNNNNNGC 4 cut(s) 474, 850, 982, 991
NciI CCSGG 1 cut(s) 254
NcoI CCATGG 1 cut(s) 1016
NdeII GATC 1 cut(s) 138
NlaIII CATG 3 cut(s) 173, 395, 1020
NlaIV GGNNCC 2 cut(s) 750, 975
NmeAIII GCCGAG 2 cut(s) 154, 494
NmuCI GTSAC 2 cut(s) 262, 314
NsbI TGCGCA 1 cut(s) 854
PceI AGGCCT 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 863
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 937
PfeI GAWTC 5 cut(s) 114, 257, 284, 305, 536
PinAI ACCGGT 1 cut(s) 318
PkrI GCNGC 8 cut(s) 545, 676, 814, 817, 885, 937, 996, 1003
PleI GAGTC 5 cut(s) 77, 235, 259, 672, 936
PpsI GAGTC 5 cut(s) 77, 235, 259, 672, 936
PpuMI RGGWCCY 1 cut(s) 748
Psp5II RGGWCCY 1 cut(s) 748
Psp6I CCWGG 2 cut(s) 66, 98
PspGI CCWGG 2 cut(s) 66, 98
PspN4I GGNNCC 2 cut(s) 750, 975
PspPI GGNCC 5 cut(s) 300, 321, 466, 748, 798
PspPPI RGGWCCY 1 cut(s) 748
PstI CTGCAG 3 cut(s) 679, 817, 885
PstNI CAGNNNCTG 1 cut(s) 880
PvuII CAGCTG 1 cut(s) 818
Rsr2I CGGWCCG 1 cut(s) 321
RsrII CGGWCCG 1 cut(s) 321
SalI GTCGAC 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 863
SaqAI TTAA 2 cut(s) 72, 764
SatI GCNGC 8 cut(s) 544, 675, 813, 816, 884, 936, 995, 1002
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 5 cut(s) 300, 321, 466, 748, 798
SchI GAGTC 5 cut(s) 77, 236, 259, 673, 937
ScrFI CCNGG 3 cut(s) 68, 100, 254
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 4 cut(s) 675, 813, 881, 978
SinI GGWCC 4 cut(s) 300, 321, 748, 798
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 6 cut(s) 176, 362, 436, 446, 486, 566
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 6 cut(s) 194, 276, 543, 844, 865, 970
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 66, 98, 252
StyI CCWWGG 1 cut(s) 1016
TaaI ACNGT 3 cut(s) 332, 551, 982
TaiI ACGT 1 cut(s) 943
TaqI TCGA 5 cut(s) 57, 81, 108, 117, 162
TaqII GACCGA 1 cut(s) 67
TasI AATT 6 cut(s) 176, 362, 436, 446, 486, 566
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 5 cut(s) 114, 257, 284, 305, 536
Tru1I TTAA 2 cut(s) 72, 764
Tru9I TTAA 2 cut(s) 72, 764
TscAI CASTG 2 cut(s) 966, 987
TseFI GTSAC 2 cut(s) 262, 314
TseI GCWGC 7 cut(s) 674, 812, 815, 883, 935, 994, 1001
Tsp45I GTSAC 2 cut(s) 262, 314
TspDTI ATGAA 3 cut(s) 651, 875, 963
TspGWI ACGGA 2 cut(s) 513, 796
TspRI CASTG 2 cut(s) 966, 987
VpaK11BI GGWCC 4 cut(s) 300, 321, 748, 798
XagI CCTNNNNNAGG 1 cut(s) 509
XapI RAATTY 4 cut(s) 176, 436, 446, 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.