MD10G1004400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008074
Physical Location & Seq
Reverse (-)
724873 .. 728307
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1004400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGACGGAGGAGGAGGAGAAGTTGGTGAGTTGCAGGACTGGGAGGTGCTAAACAGCTCGGACCCTACCCTGGTTAACTCAGACGACTCGATTGATAGCCCTAGGAGCTTCGAGGGAATCGATGTTCAAGCCGAAGGTATGATCAGGCCTGATTACTTCTCCCTGGATAATCAAGGGAGGTTCGGGTATGGTAAGGCGGTGATTGATGCCGGCGAAGAGGGTTCGGTTGAGTCCGACAACCCCAGTTGGATTGACCCGGGTTCCATGACTCAATATGGTGGAAAAGAATCGGGTGGGTTTTGGTCCGATTCGGCAAGTGATCGGTCCGATGACCGAAAAAGTCATGATTTTGAGGGGAAAAAAGAATTGGGTTTTGTGGAAAGTGAGAAAGGCCATGTGGGTTGTGGAGGGGCTGGAGAAAGGGGAGCTGTGGATGAGAAAGTTAGTGGGTTGGATGCTCAGGGCCGAGTGAGCTTTGAAGCAATTGGGGAAAACGGAAGTCTTGATAAGGATTTGGGAAAGTTCTGGTCTGATTCAGGCGGCGACAGTCTGGTTTCTGTGAAATTTGAGAAGGTTGCGAAGGAAAGCGAGATAGGTTTTAGTGGCTTGGAAAGTGGAAATAACTTGGACAATGAGCCAGAAGGACAATCGGTTGAGAGTCAAAGTAGTGCAGTTGAAGATGAGAAATCAGAGCCGAAATCCGGTGAAGAGGTAGGGAAGAGGACAATCCTGTGGTGGAAGGTCCCATTTGAGGTCTTGAAGTATTGTGTTTTCAGGGTAAGCCCTGTTTGGTCCTTGTCTGTGGCTGCAGCGGTAATGGGTTTAGTGCTCTTGGGGCGGAGATTGCACAAGATGAAGCGAAAGAGCCAGAGCTTGCAGCTCAAGGTTACTCTGGATGATAAGAAGGTGTCTCAGTTCATGAGCCGTGCTGTGCGTCTCAATGAAGCATTTTCAGTGGTGAAGCGAGTTCCTATAATGCGACCTTCGCTGCCAGCAGCTGGAATAAATCCATGGCCTGTAATGGGTTTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

345

Amino Acids

37.28

Weight (kDa)

4.78

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6821 PF20705 213 - 329 1.4e-13 Family of unknown function (DUF6821)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 998
AciI CCGC 4 cut(s) 197, 540, 812, 838
AcsI RAATTY 1 cut(s) 563
AfiI CCNNNNNNNGG 5 cut(s) 70, 701, 751, 998, 1022
AgsI TTSAA 4 cut(s) 128, 479, 677, 760
AjnI CCWGG 2 cut(s) 69, 162
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 7 cut(s) 57, 108, 428, 474, 873, 880, 998
AluI AGCT 7 cut(s) 57, 108, 428, 474, 873, 880, 998
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 2 cut(s) 915, 941
AlwNI CAGNNNCTG 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 4 cut(s) 146, 391, 463, 1013
ApeKI GCWGC 5 cut(s) 806, 809, 877, 988, 995
ApoI RAATTY 1 cut(s) 563
AspA2I CCTAGG 1 cut(s) 101
AspS9I GGNCC 6 cut(s) 61, 303, 324, 463, 742, 792
AsuC2I CCSGG 2 cut(s) 257, 258
AsuHPI GGTGA 4 cut(s) 38, 211, 716, 970
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 5 cut(s) 61, 303, 324, 742, 792
AvrII CCTAGG 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 5 cut(s) 793, 821, 889, 975, 1007
BceAI ACGGC 1 cut(s) 909
BciT130I CCWGG 2 cut(s) 71, 164
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 2 cut(s) 257, 258
BcoDI GTCTC 2 cut(s) 915, 941
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 1 cut(s) 807
BisI GCNGC 6 cut(s) 541, 807, 810, 878, 989, 996
BlnI CCTAGG 1 cut(s) 101
BlsI GCNGC 6 cut(s) 542, 808, 811, 879, 990, 997
Bme1390I CCNGG 4 cut(s) 71, 164, 257, 258
Bme18I GGWCC 5 cut(s) 61, 303, 324, 742, 792
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 61, 303, 324, 463, 742, 792
BmiI GGNNCC 3 cut(s) 63, 262, 744
BmrFI CCNGG 4 cut(s) 71, 164, 257, 258
BmrI ACTGGG 2 cut(s) 49, 237
BmsI GCATC 2 cut(s) 196, 445
BmuI ACTGGG 2 cut(s) 49, 237
BpmI CTGGAG 1 cut(s) 435
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 2 cut(s) 257, 258
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 5 cut(s) 69, 101, 162, 256, 1010
BsaWI WCCGGW 1 cut(s) 701
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 701, 751, 998, 1022
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 2 cut(s) 44, 243
BseBI CCWGG 2 cut(s) 71, 164
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 5 cut(s) 69, 101, 162, 256, 1010
BseGI GGATG 3 cut(s) 439, 460, 901
BseLI CCNNNNNNNGG 5 cut(s) 70, 701, 751, 998, 1022
BseMII CTCAG 3 cut(s) 93, 473, 926
BseNI ACTGG 2 cut(s) 44, 243
BseRI GAGGAG 3 cut(s) 24, 27, 30
BseXI GCAGC 5 cut(s) 793, 821, 889, 975, 1007
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 4 cut(s) 148, 393, 465, 1015
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 3 cut(s) 210, 257, 702
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 5 cut(s) 70, 701, 751, 998, 1022
BsmAI GTCTC 2 cut(s) 915, 941
BsmBI CGTCTC 1 cut(s) 941
BsmFI GGGAC 1 cut(s) 728
BsnI GGCC 4 cut(s) 148, 393, 465, 1015
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 831
Bsp143I GATC 2 cut(s) 141, 319
Bsp19I CCATGG 1 cut(s) 1010
BspACI CCGC 4 cut(s) 197, 540, 812, 838
BspANI GGCC 4 cut(s) 148, 393, 465, 1015
BspCNI CTCAG 3 cut(s) 92, 472, 925
BspDI ATCGAT 1 cut(s) 120
BspHI TCATGA 2 cut(s) 343, 918
BspLI GGNNCC 3 cut(s) 63, 262, 744
BspMAI CTGCAG 1 cut(s) 811
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 2 cut(s) 44, 243
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 5 cut(s) 69, 101, 162, 256, 1010
BssMI GATC 2 cut(s) 141, 319
BssT1I CCWWGG 2 cut(s) 101, 1010
Bst2UI CCWGG 2 cut(s) 71, 164
Bst4CI ACNGT 1 cut(s) 548
Bst6I CTCTTC 3 cut(s) 210, 702, 713
BstC8I GCNNGC 3 cut(s) 211, 875, 993
BstDEI CTNAG 3 cut(s) 79, 459, 912
BstDSI CCRYGG 1 cut(s) 1010
BstF5I GGATG 3 cut(s) 439, 460, 901
BstKTI GATC 2 cut(s) 144, 322
BstMAI GTCTC 2 cut(s) 915, 941
BstMBI GATC 2 cut(s) 141, 319
BstMWI GCNNNNNNNGC 5 cut(s) 105, 471, 835, 844, 985
BstNI CCWGG 2 cut(s) 71, 164
BstSCI CCNGG 4 cut(s) 69, 162, 255, 256
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 5 cut(s) 793, 821, 889, 975, 1007
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 4 cut(s) 148, 393, 465, 1015
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 1010
BtsCI GGATG 3 cut(s) 439, 460, 901
BtsIMutI CAGTG 1 cut(s) 960
Cac8I GCNNGC 3 cut(s) 211, 875, 993
CaiI CAGNNNCTG 1 cut(s) 998
CciI TCATGA 2 cut(s) 343, 918
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 6 cut(s) 61, 303, 324, 463, 742, 792
Cfr9I CCCGGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 324
CseI GACGC 1 cut(s) 923
CspI CGGWCCG 1 cut(s) 324
CviAII CATG 5 cut(s) 265, 344, 395, 919, 1011
DdeI CTNAG 3 cut(s) 79, 459, 912
DpnI GATC 2 cut(s) 143, 321
DpnII GATC 2 cut(s) 141, 319
Eam1104I CTCTTC 3 cut(s) 210, 702, 713
EarI CTCTTC 3 cut(s) 210, 702, 713
EciI GGCGGA 1 cut(s) 853
Eco130I CCWWGG 2 cut(s) 101, 1010
Eco147I AGGCCT 1 cut(s) 148
Eco47I GGWCC 5 cut(s) 61, 303, 324, 742, 792
Eco88I CYCGRG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 69, 162
EcoT14I CCWWGG 2 cut(s) 101, 1010
ErhI CCWWGG 2 cut(s) 101, 1010
Esp3I CGTCTC 1 cut(s) 941
FaeI CATG 5 cut(s) 268, 347, 398, 922, 1014
FaiI YATR 9 cut(s) 140, 189, 266, 276, 345, 396, 920, 974, 1012
FaqI GGGAC 1 cut(s) 728
FatI CATG 5 cut(s) 264, 343, 394, 918, 1010
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 541, 807, 810, 878, 989, 996
FokI GGATG 3 cut(s) 446, 467, 908
Fsp4HI GCNGC 6 cut(s) 541, 807, 810, 878, 989, 996
FspBI CTAG 1 cut(s) 102
GluI GCNGC 6 cut(s) 541, 807, 810, 878, 989, 996
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 4 cut(s) 148, 393, 465, 1015
HapII CCGG 3 cut(s) 210, 257, 702
HgaI GACGC 1 cut(s) 923
Hin1II CATG 5 cut(s) 268, 347, 398, 922, 1014
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 8 cut(s) 86, 117, 230, 268, 287, 308, 533, 658
HpaI GTTAAC 1 cut(s) 76
HpaII CCGG 3 cut(s) 210, 257, 702
HphI GGTGA 4 cut(s) 38, 211, 716, 970
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 7 cut(s) 61, 82, 235, 307, 328, 532, 691
Hpy188III TCNNGA 5 cut(s) 344, 503, 757, 893, 919
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 7 cut(s) 128, 565, 574, 635, 733, 898, 993
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4V TGCA 5 cut(s) 34, 671, 809, 847, 877
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 471, 835, 844, 985
HpyF3I CTNAG 3 cut(s) 79, 459, 912
Hsp92II CATG 5 cut(s) 268, 347, 398, 922, 1014
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 141
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 2 cut(s) 141, 319
LmnI GCTCC 2 cut(s) 105, 425
Lsp1109I GCAGC 5 cut(s) 793, 821, 889, 975, 1007
LweI GCATC 2 cut(s) 196, 445
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 886
MalI GATC 2 cut(s) 143, 321
MboI GATC 2 cut(s) 141, 319
MboII GAAGA 4 cut(s) 227, 689, 719, 730
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 831
MluCI AATT 3 cut(s) 365, 483, 563
MlyI GAGTC 4 cut(s) 80, 239, 262, 667
MmeI TCCRAC 3 cut(s) 227, 258, 432
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 1 cut(s) 75
MspA1I CMGCKG 2 cut(s) 812, 998
MspI CCGG 3 cut(s) 210, 257, 702
MspR9I CCNGG 4 cut(s) 71, 164, 257, 258
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 71, 164
MwoI GCNNNNNNNGC 5 cut(s) 105, 471, 835, 844, 985
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 2 cut(s) 257, 258
NcoI CCATGG 1 cut(s) 1010
NdeII GATC 2 cut(s) 141, 319
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 5 cut(s) 268, 347, 398, 922, 1014
NlaIV GGNNCC 3 cut(s) 63, 262, 744
NmeAIII GCCGAG 1 cut(s) 491
PagI TCATGA 2 cut(s) 343, 918
PceI AGGCCT 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 117, 287, 308, 533
PflMI CCANNNNNTGG 1 cut(s) 998
PkrI GCNGC 6 cut(s) 542, 808, 811, 879, 990, 997
PleI GAGTC 4 cut(s) 80, 238, 262, 666
PpsI GAGTC 4 cut(s) 80, 238, 262, 666
PpuMI RGGWCCY 1 cut(s) 742
Psp5II RGGWCCY 1 cut(s) 742
Psp6I CCWGG 2 cut(s) 69, 162
PspGI CCWGG 2 cut(s) 69, 162
PspN4I GGNNCC 3 cut(s) 63, 262, 744
PspPI GGNCC 6 cut(s) 61, 303, 324, 463, 742, 792
PspPPI RGGWCCY 1 cut(s) 742
PstI CTGCAG 1 cut(s) 811
PstNI CAGNNNCTG 1 cut(s) 998
PvuII CAGCTG 1 cut(s) 998
Rsr2I CGGWCCG 1 cut(s) 324
RsrII CGGWCCG 1 cut(s) 324
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 6 cut(s) 541, 807, 810, 878, 989, 996
Sau3AI GATC 2 cut(s) 141, 319
Sau96I GGNCC 6 cut(s) 61, 303, 324, 463, 742, 792
SchI GAGTC 4 cut(s) 80, 239, 262, 667
ScrFI CCNGG 4 cut(s) 71, 164, 257, 258
SduI GDGCHC 1 cut(s) 831
SfaNI GCATC 2 cut(s) 196, 445
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 5 cut(s) 61, 303, 324, 742, 792
SmaI CCCGGG 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
Sse9I AATT 3 cut(s) 365, 483, 563
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 4 cut(s) 197, 540, 812, 838
SspMI CTAG 1 cut(s) 102
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 4 cut(s) 69, 162, 255, 256
StyI CCWWGG 2 cut(s) 101, 1010
TaaI ACNGT 1 cut(s) 548
TaqI TCGA 3 cut(s) 89, 111, 120
TaqII GACCGA 2 cut(s) 312, 348
TasI AATT 3 cut(s) 365, 483, 563
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 4 cut(s) 117, 287, 308, 533
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 960
TseI GCWGC 5 cut(s) 806, 809, 877, 988, 995
TspDTI ATGAA 3 cut(s) 869, 907, 957
TspGWI ACGGA 2 cut(s) 21, 510
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 1 cut(s) 960
Van91I CCANNNNNTGG 1 cut(s) 998
VpaK11BI GGWCC 5 cut(s) 61, 303, 324, 742, 792
XapI RAATTY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 256
XmaJI CCTAGG 1 cut(s) 101
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.