FvH4_2g01060

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009103
Physical Location & Seq
Forward (+)
1035120 .. 1037475
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01060.t1

Sequence Viewer

Length: 762 bp
ATGGCCTGTCATTGTGCTTATCATCCATTTCCACCATCACCATTGAGGAGTTTCAACCCCAACAACAAGACCAGTCTTCTTCTTGCTTCATCAAAAAGACCCAATTCTGTCTTGCTTGCTGCCGTCAGCCACAATAGAGAGTCTTACAAGACCTTTGTCTCTGAGGCTGCTAGGCTTTTGGGTCCAGCAAGATTTGACGCTTCAAAGCTAAAGGTTGAGTTCATGGGAGAAGATATGAACAACTATGTTGGAATCATCCCCAGGACTTACATACTATCTCATTGTGACTTCACAGCTAACTTGACCCTAACAATCTCCAATGTCATCAACCTCGAACAGTTAAAAGGATGGTACAGCAAGGATGATGTTGTTGCAGAGTGGAAAAGGGTGAATGGTGAAATGTGTCTGCATATCCATTGCTATGTAAGCGGTCCCAATCTCTTGCTAGACCTGGCAGCGGAGTTCAGATATCACATATTTTCCAAAGAAATGCCTTTGGTACTGAAAGCTGTACTACATGGAGACTCGGCACTTTTCAGAGAGCATCCAGAGCTACGTGATTCTTTAGTTCGGGTTTATTTTCATTCTAGTTTGAAGAAGTACAATAGAATTGAATGCTGGGGGCCCCTCAAAGATGCTGTAGAGGAAATACCAAGGGATTGCAGTCAAGGAGTAATTGCATCTATCGATCCCCTCCGTCCTCCAGACAAGTGGGCAAGGCCAAAAACCTTATTTCAAGTTCTATTTGCGTTCCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

254

Amino Acids

28.82

Weight (kDa)

8.62

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 64 - 213 1.5e-59 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 429, 458
AclWI GGATC 1 cut(s) 683
AfaI GTAC 4 cut(s) 353, 501, 513, 602
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 5 cut(s) 55, 204, 595, 614, 737
AjnI CCWGG 2 cut(s) 260, 450
AluBI AGCT 4 cut(s) 208, 296, 509, 553
AluI AGCT 4 cut(s) 208, 296, 509, 553
Alw26I GTCTC 2 cut(s) 163, 516
AlwI GGATC 1 cut(s) 683
AoxI GGCC 3 cut(s) 3, 623, 719
ApaI GGGCCC 1 cut(s) 627
ApeKI GCWGC 3 cut(s) 119, 167, 455
AspS9I GGNCC 4 cut(s) 182, 431, 623, 624
AsuHPI GGTGA 3 cut(s) 30, 400, 407
AvaII GGWCC 2 cut(s) 182, 431
BaeGI GKGCMC 1 cut(s) 627
BanII GRGCYC 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 106, 154, 467
BccI CCATC 2 cut(s) 43, 342
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 2 cut(s) 262, 452
BcoDI GTCTC 2 cut(s) 163, 516
BfaI CTAG 3 cut(s) 171, 446, 588
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 3 cut(s) 120, 168, 456
BlsI GCNGC 3 cut(s) 121, 169, 457
Bme1390I CCNGG 2 cut(s) 262, 452
Bme18I GGWCC 2 cut(s) 182, 431
BmgT120I GGNCC 4 cut(s) 182, 431, 623, 624
BmiI GGNNCC 5 cut(s) 183, 433, 624, 625, 626
BmrFI CCNGG 2 cut(s) 262, 452
BmsI GCATC 3 cut(s) 553, 625, 689
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 687
Bsa29I ATCGAT 1 cut(s) 687
BsaAI YACGTR 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 260, 653
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 2 cut(s) 262, 452
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 260, 653
BseGI GGATG 5 cut(s) 22, 255, 353, 367, 544
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 61
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 3 cut(s) 106, 154, 467
BseYI CCCAGC 1 cut(s) 618
BshFI GGCC 3 cut(s) 5, 625, 721
BshVI ATCGAT 1 cut(s) 687
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 163, 516
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 3 cut(s) 5, 625, 721
Bsp120I GGGCCC 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 1 cut(s) 688
BspACI CCGC 2 cut(s) 429, 458
BspANI GGCC 3 cut(s) 5, 625, 721
BspCNI CTCAG 1 cut(s) 154
BspDI ATCGAT 1 cut(s) 687
BspLI GGNNCC 5 cut(s) 183, 433, 624, 625, 626
BspPI GGATC 1 cut(s) 683
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 260, 653
BssMI GATC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 2 cut(s) 262, 452
Bst4CI ACNGT 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 5 cut(s) 22, 255, 353, 367, 544
BstKTI GATC 1 cut(s) 691
BstMAI GTCTC 2 cut(s) 163, 516
BstMBI GATC 1 cut(s) 688
BstMWI GCNNNNNNNGC 2 cut(s) 426, 550
BstNI CCWGG 2 cut(s) 262, 452
BstPAI GACNNNNGTC 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 260, 450
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 3 cut(s) 106, 154, 467
BstV2I GAAGAC 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 711
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 3 cut(s) 5, 625, 721
BsuTUI ATCGAT 1 cut(s) 687
BtsCI GGATG 5 cut(s) 22, 255, 353, 367, 544
Cac8I GCNNGC 1 cut(s) 117
Cfr13I GGNCC 4 cut(s) 182, 431, 623, 624
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 352, 500, 512, 601
CviAII CATG 2 cut(s) 223, 518
CviQI GTAC 4 cut(s) 352, 500, 512, 601
DdeI CTNAG 1 cut(s) 162
DpnI GATC 1 cut(s) 690
DpnII GATC 1 cut(s) 688
Eco130I CCWWGG 1 cut(s) 653
Eco24I GRGCYC 1 cut(s) 627
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 182, 431
EcoO109I RGGNCCY 2 cut(s) 623, 624
EcoRII CCWGG 2 cut(s) 260, 450
EcoRV GATATC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 2 cut(s) 226, 521
FaiI YATR 8 cut(s) 224, 236, 246, 272, 411, 423, 476, 519
FaqI GGGAC 1 cut(s) 417
FatI CATG 2 cut(s) 222, 517
Fnu4HI GCNGC 3 cut(s) 120, 168, 456
FokI GGATG 5 cut(s) 9, 242, 360, 374, 531
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 3 cut(s) 120, 168, 456
FspBI CTAG 3 cut(s) 171, 446, 588
GluI GCNGC 3 cut(s) 120, 168, 456
GsaI CCCAGC 1 cut(s) 622
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 3 cut(s) 5, 625, 721
HgaI GACGC 1 cut(s) 206
Hin1II CATG 2 cut(s) 226, 521
HinfI GANTC 4 cut(s) 140, 252, 524, 560
HphI GGTGA 3 cut(s) 30, 400, 407
Hpy188I TCNGA 3 cut(s) 163, 467, 539
Hpy188III TCNNGA 2 cut(s) 548, 704
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 556
HpyCH4V TGCA 4 cut(s) 374, 409, 663, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 550
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 2 cut(s) 226, 521
Kzo9I GATC 1 cut(s) 688
Lsp1109I GCAGC 3 cut(s) 106, 154, 467
LweI GCATC 3 cut(s) 553, 625, 689
MaeI CTAG 3 cut(s) 171, 446, 588
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 1 cut(s) 284
MalI GATC 1 cut(s) 690
MboI GATC 1 cut(s) 688
MboII GAAGA 4 cut(s) 68, 71, 242, 607
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 3 cut(s) 103, 609, 675
MlyI GAGTC 2 cut(s) 149, 518
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 7 cut(s) 39, 157, 341, 637, 638, 704, 711
MseI TTAA 1 cut(s) 341
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 458
MspR9I CCNGG 2 cut(s) 262, 452
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 2 cut(s) 262, 452
MwoI GCNNNNNNNGC 2 cut(s) 426, 550
NdeII GATC 1 cut(s) 688
NlaIII CATG 2 cut(s) 226, 521
NlaIV GGNNCC 5 cut(s) 183, 433, 624, 625, 626
NmeAIII GCCGAG 1 cut(s) 506
NmuCI GTSAC 1 cut(s) 284
PctI GAATGC 1 cut(s) 620
PfeI GAWTC 2 cut(s) 252, 560
PkrI GCNGC 3 cut(s) 121, 169, 457
PleI GAGTC 2 cut(s) 148, 518
PpsI GAGTC 2 cut(s) 148, 518
Ppu21I YACGTR 1 cut(s) 557
PshAI GACNNNNGTC 1 cut(s) 155
Psp6I CCWGG 2 cut(s) 260, 450
PspFI CCCAGC 1 cut(s) 618
PspGI CCWGG 2 cut(s) 260, 450
PspN4I GGNNCC 5 cut(s) 183, 433, 624, 625, 626
PspOMI GGGCCC 1 cut(s) 623
PspPI GGNCC 4 cut(s) 182, 431, 623, 624
RsaI GTAC 4 cut(s) 353, 501, 513, 602
RsaNI GTAC 4 cut(s) 352, 500, 512, 601
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 3 cut(s) 120, 168, 456
Sau3AI GATC 1 cut(s) 688
Sau96I GGNCC 4 cut(s) 182, 431, 623, 624
SchI GAGTC 2 cut(s) 149, 518
ScrFI CCNGG 2 cut(s) 262, 452
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 3 cut(s) 553, 625, 689
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 2 cut(s) 182, 431
SmiMI CAYNNNNRTG 1 cut(s) 420
Sse9I AATT 3 cut(s) 103, 609, 675
SsiI CCGC 2 cut(s) 429, 458
SspMI CTAG 3 cut(s) 171, 446, 588
StyD4I CCNGG 2 cut(s) 260, 450
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 1 cut(s) 559
TaqI TCGA 2 cut(s) 333, 687
TasI AATT 3 cut(s) 103, 609, 675
TatI WGTACW 2 cut(s) 511, 600
TfiI GAWTC 2 cut(s) 252, 560
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 3 cut(s) 119, 167, 455
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 78, 211, 251, 572
TspGWI ACGGA 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 182, 431
XspI CTAG 3 cut(s) 171, 446, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.