Rh6BG000400

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009103
Physical Location & Seq
Reverse (-)
80760 .. 83004
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG000400.1

Sequence Viewer

Length: 666 bp
ATGGCCTGTCATTGTGCTTATCATTCATTTCCACCATCACCATTGAGGAGCTTCAGCAACAACAACAACAACAACAACAAGACCAGTCTTCTTCTTGCTTCATCAAAAAGACCCAATTCTCTTTTGCTTTCTGCCATCAGCCACAATAGAGAATCTTACAGTACCTTTGTTTCTGAGGCTGCTAGGCTTTTGGGTCCAGCAAGATTTGACGCTTCAAAGCTAAAGGTTGAGTTTATTGGAGAAGAGATGAACAACTATGTCGGAATCATCCCAAGAACTTACATCCTATCTCATTGTGACTTCACAGCTAACTTGACCTTAACCATCTCCAATGTCATCAACCTTGAACAGTTAAAAGGATGGTACAGCAAGGATGATGTTGTTGCAGAGTGGAAAAGGGTGAATGGTGAAATGTGTCTACATATCCATTGCTATGTGAGCGGTCCCAATCTCTTGCTAGACCTGGCAGCGGAGTTCAGATATCACATATTTTCCAAAGAAATGCCTTTGGTAAGTATTTCTTCTATAATTGCAATGCCCTTCTGTATTACAAAGGAATATATATTTAGGACTAAAGGATATGAACTCACTATATTGAGACTCACTACTTCCCCTGCAGTTCTTTTTATAGGTTCTGTAGATGCTAAAGGATATTCTGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

221

Amino Acids

24.78

Weight (kDa)

8.18

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 68 - 171 2.3e-35 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 441
AccI GTMKAC 1 cut(s) 418
AciI CCGC 2 cut(s) 441, 470
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 2 cut(s) 163, 365
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 2 cut(s) 216, 347
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 3 cut(s) 51, 220, 308
AluI AGCT 3 cut(s) 51, 220, 308
Alw26I GTCTC 1 cut(s) 592
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 179, 467
AspS9I GGNCC 2 cut(s) 194, 443
AsuHPI GGTGA 3 cut(s) 30, 412, 419
AvaII GGWCC 2 cut(s) 194, 443
BbsI GAAGAC 1 cut(s) 80
BbvI GCAGC 2 cut(s) 166, 479
BccI CCATC 4 cut(s) 43, 143, 332, 354
BciT130I CCWGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 2 cut(s) 183, 458
BfmI CTRYAG 2 cut(s) 615, 636
BisI GCNGC 2 cut(s) 180, 468
BlsI GCNGC 2 cut(s) 181, 469
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 2 cut(s) 194, 443
BmgT120I GGNCC 2 cut(s) 194, 443
BmiI GGNNCC 2 cut(s) 195, 445
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 1 cut(s) 631
BpiI GAAGAC 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse1I ACTGG 1 cut(s) 84
Bse3DI GCAATG 2 cut(s) 427, 540
BseBI CCWGG 1 cut(s) 464
BseGI GGATG 4 cut(s) 267, 282, 365, 379
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMI GCAATG 2 cut(s) 427, 540
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 84
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 166, 479
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 469
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 1 cut(s) 429
BsnI GGCC 1 cut(s) 5
BspACI CCGC 2 cut(s) 441, 470
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 166
BspLI GGNNCC 2 cut(s) 195, 445
BspMAI CTGCAG 1 cut(s) 619
BsrBI CCGCTC 1 cut(s) 441
BsrDI GCAATG 2 cut(s) 427, 540
BsrI ACTGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 161, 351
Bst6I CTCTTC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 4 cut(s) 267, 282, 365, 379
BstMAI GTCTC 1 cut(s) 592
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNI CCWGG 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 2 cut(s) 615, 636
BstV1I GCAGC 2 cut(s) 166, 479
BstV2I GAAGAC 1 cut(s) 80
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 4 cut(s) 267, 282, 365, 379
Cfr13I GGNCC 2 cut(s) 194, 443
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 162, 364
CviJI RGCY 7 cut(s) 5, 51, 141, 179, 187, 220, 308
CviKI_1 RGCY 7 cut(s) 5, 51, 141, 179, 187, 220, 308
CviQI GTAC 2 cut(s) 162, 364
DdeI CTNAG 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 482
Eco47I GGWCC 2 cut(s) 194, 443
Eco57I CTGAAG 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 482
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FaqI GGGAC 1 cut(s) 429
FblI GTMKAC 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 180, 468
FokI GGATG 4 cut(s) 254, 269, 372, 386
Fsp4HI GCNGC 2 cut(s) 180, 468
FspBI CTAG 2 cut(s) 183, 458
GluI GCNGC 2 cut(s) 180, 468
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 218
HinfI GANTC 3 cut(s) 152, 264, 600
HphI GGTGA 3 cut(s) 30, 412, 419
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 3 cut(s) 175, 263, 479
Hpy8I GTNNAC 1 cut(s) 419
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 161, 351
HpyCH4V TGCA 3 cut(s) 386, 533, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 1 cut(s) 174
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 7 cut(s) 19, 97, 210, 449, 476, 627, 642
Lsp1109I GCAGC 2 cut(s) 166, 479
LweI GCATC 1 cut(s) 631
MaeI CTAG 2 cut(s) 183, 458
MaeIII GTNAC 2 cut(s) 296, 659
MbiI CCGCTC 1 cut(s) 441
MboII GAAGA 4 cut(s) 80, 83, 254, 513
MluCI AATT 2 cut(s) 115, 528
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 2 cut(s) 39, 169
MseI TTAA 2 cut(s) 320, 353
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 470
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 438
NlaIV GGNNCC 2 cut(s) 195, 445
NmuCI GTSAC 2 cut(s) 296, 659
PfeI GAWTC 2 cut(s) 152, 264
PkrI GCNGC 2 cut(s) 181, 469
PleI GAGTC 1 cut(s) 594
PpsI GAGTC 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 195, 445
PspPI GGNCC 2 cut(s) 194, 443
PstI CTGCAG 1 cut(s) 619
RsaI GTAC 2 cut(s) 163, 365
RsaNI GTAC 2 cut(s) 162, 364
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 2 cut(s) 320, 353
SatI GCNGC 2 cut(s) 180, 468
Sau96I GGNCC 2 cut(s) 194, 443
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 1 cut(s) 464
SetI ASST 9 cut(s) 53, 167, 222, 228, 310, 320, 345, 465, 634
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 2 cut(s) 615, 636
SinI GGWCC 2 cut(s) 194, 443
SmiMI CAYNNNNRTG 1 cut(s) 432
Sse9I AATT 2 cut(s) 115, 528
SsiI CCGC 2 cut(s) 441, 470
SspMI CTAG 2 cut(s) 183, 458
StyD4I CCNGG 1 cut(s) 462
TaaI ACNGT 2 cut(s) 161, 351
TasI AATT 2 cut(s) 115, 528
TfiI GAWTC 2 cut(s) 152, 264
Tru1I TTAA 2 cut(s) 320, 353
Tru9I TTAA 2 cut(s) 320, 353
TseFI GTSAC 2 cut(s) 296, 659
TseI GCWGC 2 cut(s) 179, 467
Tsp45I GTSAC 2 cut(s) 296, 659
TspDTI ATGAA 4 cut(s) 15, 90, 263, 597
VpaK11BI GGWCC 2 cut(s) 194, 443
XmiI GTMKAC 1 cut(s) 418
XspI CTAG 2 cut(s) 183, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.