FvH4_2g05260

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009103
Physical Location & Seq
Reverse (-)
4343689 .. 4344395
707 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05260.t1

Sequence Viewer

Length: 453 bp
ATGCTTACATGTTATAATTGGCAGGCTGCTAGGCTTTTGGGTCCAGCAAGATTTGACGCTTCAAAGCTAAAGGTTGAGTTTATGGGAGAAGGGATGAACAACTATGTTGGAATCATGCCAAGAACTTACATCCTATCTCATTGTGACTTCACAGCTAACTTGACCTTAACAATCTCCAATGTCATCAACCTTGAACAATTAAAAGGATGGTACAGCAAGGATGATGTTGTTGTAGAGTGGAAAAGGGTGATTGGTGAAATGTGTCTGCATATCCATTGCTATGTGAGCGGTCTCAATCTCTTGCTAGACCTGGCAGCGGAGTTCAGATATCACATATTTTCCAAAGAAATGCCTTTGGTACTGAAAGCTGTGCTACATGGAGACTCGCCACTTTTCAGAGAGCATCCAGAGCTACGTGATGCTTTAGTTCGGGTTTATTTTCATTCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

151

Amino Acids

17.31

Weight (kDa)

6.64

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 17 - 150 3e-49 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 15
AccBSI CCGCTC 1 cut(s) 288
AciI CCGC 2 cut(s) 288, 317
AfaI GTAC 2 cut(s) 212, 360
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 8
AgsI TTSAA 2 cut(s) 63, 194
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 5 cut(s) 67, 155, 368, 412, 450
AluI AGCT 5 cut(s) 67, 155, 368, 412, 450
Alw26I GTCTC 2 cut(s) 296, 375
ApeKI GCWGC 2 cut(s) 26, 314
AspS9I GGNCC 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 259, 266
AvaII GGWCC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 13, 326
BccI CCATC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 2 cut(s) 296, 375
BfaI CTAG 4 cut(s) 30, 305, 447, 451
BisI GCNGC 2 cut(s) 27, 315
BlsI GCNGC 2 cut(s) 28, 316
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 2 cut(s) 409, 412
BsaAI YACGTR 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 311
BseGI GGATG 5 cut(s) 99, 129, 212, 226, 403
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 274
BseXI GCAGC 2 cut(s) 13, 326
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 296, 375
Bso31I GGTCTC 1 cut(s) 296
BspACI CCGC 2 cut(s) 288, 317
BspLI GGNNCC 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 311
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 24
BstF5I GGATG 5 cut(s) 99, 129, 212, 226, 403
BstMAI GTCTC 2 cut(s) 296, 375
BstMWI GCNNNNNNNGC 2 cut(s) 285, 409
BstNI CCWGG 1 cut(s) 311
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 13, 326
BtsCI GGATG 5 cut(s) 99, 129, 212, 226, 403
Cac8I GCNNGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 41
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 211, 359
CviAII CATG 3 cut(s) 9, 115, 377
CviJI RGCY 7 cut(s) 26, 34, 67, 155, 368, 412, 450
CviKI_1 RGCY 7 cut(s) 26, 34, 67, 155, 368, 412, 450
CviQI GTAC 2 cut(s) 211, 359
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 309
EcoRV GATATC 1 cut(s) 329
FaeI CATG 3 cut(s) 12, 118, 380
FaiI YATR 9 cut(s) 10, 15, 83, 105, 116, 270, 282, 335, 378
FatI CATG 3 cut(s) 8, 114, 376
Fnu4HI GCNGC 2 cut(s) 27, 315
FokI GGATG 5 cut(s) 106, 116, 219, 233, 390
Fsp4HI GCNGC 2 cut(s) 27, 315
FspBI CTAG 4 cut(s) 30, 305, 447, 451
GluI GCNGC 2 cut(s) 27, 315
HgaI GACGC 1 cut(s) 65
Hin1II CATG 3 cut(s) 12, 118, 380
HinfI GANTC 2 cut(s) 111, 383
HphI GGTGA 2 cut(s) 259, 266
Hpy188I TCNGA 2 cut(s) 326, 398
Hpy188III TCNNGA 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 409
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 3 cut(s) 12, 118, 380
LpnPI CCDG 5 cut(s) 8, 57, 296, 323, 420
Lsp1109I GCAGC 2 cut(s) 13, 326
LweI GCATC 2 cut(s) 409, 412
MaeI CTAG 4 cut(s) 30, 305, 447, 451
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 1 cut(s) 143
MbiI CCGCTC 1 cut(s) 288
MluCI AATT 2 cut(s) 16, 197
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 1 cut(s) 88
MseI TTAA 2 cut(s) 167, 200
MslI CAYNNNNRTG 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MwoI GCNNNNNNNGC 2 cut(s) 285, 409
NlaIII CATG 3 cut(s) 12, 118, 380
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 12
PciI ACATGT 1 cut(s) 8
PfeI GAWTC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 28, 316
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Ppu21I YACGTR 1 cut(s) 416
PscI ACATGT 1 cut(s) 8
PsiI TTATAA 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 1 cut(s) 41
RsaI GTAC 2 cut(s) 212, 360
RsaNI GTAC 2 cut(s) 211, 359
RseI CAYNNNNRTG 1 cut(s) 279
SaqAI TTAA 2 cut(s) 167, 200
SatI GCNGC 2 cut(s) 27, 315
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 311
SfaNI GCATC 2 cut(s) 409, 412
SinI GGWCC 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 279
Sse9I AATT 2 cut(s) 16, 197
SsiI CCGC 2 cut(s) 288, 317
SspMI CTAG 4 cut(s) 30, 305, 447, 451
StyD4I CCNGG 1 cut(s) 309
TaiI ACGT 1 cut(s) 418
TasI AATT 2 cut(s) 16, 197
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 2 cut(s) 167, 200
Tru9I TTAA 2 cut(s) 167, 200
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 2 cut(s) 26, 314
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 110, 431
VpaK11BI GGWCC 1 cut(s) 41
XceI RCATGY 1 cut(s) 12
XspI CTAG 4 cut(s) 30, 305, 447, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.