MD05G1008700.v1.1

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009103
Physical Location & Seq
Forward (+)
1873351 .. 1875933
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1008700.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGCCACTCATTGTGCTTATTATGTATTTCCACCATCGCCACTGAGGACCTTGAACAAAACCACAACTTTTGTCTCCCCAAAAAAACCCAATAAGTCTGTTTTGCTTTCTTCCATCAGCAACAACACAGACTCCTACAATGCCTTTGTTTCTGAGGCCGCTAGTCTTTTGGGTCCAGCGAGATTCGAAGCCTCCAAACTAAAAGTTGAATTTATGGGAGAAGAGATGAATAATTATGTTGGAATCACCCCGAGAACATATATCCTATCTCACTGTGACTTCACAGCTAACCTGACCTTAAAGATCTCCAATGTCATCAACCTCGATCAGCTAAAAGGATGGTACAGCAAGGATGACGTCGTTGCAGAATGGAAAAAGGTGAATGGTGAAATGTGCCTGCATATCCATTGCTATGTGAGCGGTCCCAATATGCTTAACCTGGCTGCCGAGTTCAGATATCACATCTTTTCCAAAGAAATGCCTTTGGTACTCAAAGCTGTGTTACATGGAGACTCGCTACTTTTCAGAGAGCATCCCGAACTACCGAATGCTTTGGTTCGGGTTTACTTTCATTCTAGTTTGAAGAAGTACAACAGAATTGAATGCTGGGGCCCTCTCAAGGATGCTGTAGAGGGAAGACAAGGGGATCACACCAAACGAATAGCTGCACGTACAGATTCTTCCCATCCTCGGGACAAGTGGGGAACGCCAAAATCCATATTTCAAGTTCTATTTGCATTCCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

250

Amino Acids

28.27

Weight (kDa)

9.1

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 61 - 209 1.9e-58 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 420
AciI CCGC 2 cut(s) 159, 420
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 4 cut(s) 344, 489, 590, 673
AfiI CCNNNNNNNGG 3 cut(s) 619, 690, 691
AgsI TTSAA 5 cut(s) 55, 209, 583, 602, 725
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 4 cut(s) 287, 331, 497, 665
AluI AGCT 4 cut(s) 287, 331, 497, 665
Alw26I GTCTC 2 cut(s) 79, 504
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 2 cut(s) 250, 690
AoxI GGCC 3 cut(s) 3, 156, 610
ApaI GGGCCC 1 cut(s) 614
ApeKI GCWGC 2 cut(s) 443, 665
ApoI RAATTY 1 cut(s) 209
AspS9I GGNCC 5 cut(s) 48, 173, 422, 610, 611
AsuHPI GGTGA 3 cut(s) 238, 391, 398
AsuII TTCGAA 1 cut(s) 186
AvaI CYCGRG 2 cut(s) 250, 690
AvaII GGWCC 3 cut(s) 48, 173, 422
BaeGI GKGCMC 1 cut(s) 614
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 614
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbsI GAAGAC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 430, 652
BccI CCATC 4 cut(s) 43, 122, 333, 693
BciT130I CCWGG 1 cut(s) 440
BcoDI GTCTC 2 cut(s) 79, 504
BfaI CTAG 2 cut(s) 162, 576
BfmI CTRYAG 1 cut(s) 627
BglII AGATCT 1 cut(s) 303
BisI GCNGC 3 cut(s) 159, 444, 666
BlsI GCNGC 3 cut(s) 160, 445, 667
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 3 cut(s) 48, 173, 422
BmeT110I CYCGRG 2 cut(s) 250, 690
BmgT120I GGNCC 5 cut(s) 48, 173, 422, 610, 611
BmiI GGNNCC 4 cut(s) 174, 424, 611, 612
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 2 cut(s) 541, 613
BpiI GAAGAC 1 cut(s) 643
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 602
BsaAI YACGTR 1 cut(s) 671
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 689
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 3 cut(s) 619, 690, 691
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 689
BseGI GGATG 5 cut(s) 344, 358, 532, 628, 685
BseLI CCNNNNNNNGG 3 cut(s) 619, 690, 691
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 35, 144
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 2 cut(s) 430, 652
BseYI CCCAGC 1 cut(s) 606
BsgI GTGCAG 1 cut(s) 651
BshFI GGCC 3 cut(s) 5, 158, 612
BsiHKCI CYCGRG 2 cut(s) 250, 690
BslFI GGGAC 2 cut(s) 408, 707
BslI CCNNNNNNNGG 3 cut(s) 619, 690, 691
BsmAI GTCTC 2 cut(s) 79, 504
BsmFI GGGAC 2 cut(s) 408, 707
BsmI GAATGC 3 cut(s) 553, 608, 737
BsnI GGCC 3 cut(s) 5, 158, 612
BsoBI CYCGRG 2 cut(s) 250, 690
Bsp119I TTCGAA 1 cut(s) 186
Bsp120I GGGCCC 1 cut(s) 610
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 3 cut(s) 303, 325, 646
BspACI CCGC 2 cut(s) 159, 420
BspANI GGCC 3 cut(s) 5, 158, 612
BspCNI CTCAG 2 cut(s) 36, 145
BspLI GGNNCC 4 cut(s) 174, 424, 611, 612
BspPI GGATC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 689
BssMI GATC 3 cut(s) 303, 325, 646
BssNI GRCGYC 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 2 cut(s) 44, 153
BstF5I GGATG 5 cut(s) 344, 358, 532, 628, 685
BstKTI GATC 3 cut(s) 306, 328, 649
BstMAI GTCTC 2 cut(s) 79, 504
BstMBI GATC 3 cut(s) 303, 325, 646
BstMWI GCNNNNNNNGC 1 cut(s) 417
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 614
BstV1I GCAGC 2 cut(s) 430, 652
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuRI GGCC 3 cut(s) 5, 158, 612
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 5 cut(s) 344, 358, 532, 628, 685
BtsIMutI CAGTG 2 cut(s) 41, 271
Cac8I GCNNGC 1 cut(s) 398
Cfr13I GGNCC 5 cut(s) 48, 173, 422, 610, 611
Csp6I GTAC 4 cut(s) 343, 488, 589, 672
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 1 cut(s) 506
CviJI RGCY 9 cut(s) 5, 158, 191, 287, 331, 443, 497, 612, 665
CviKI_1 RGCY 9 cut(s) 5, 158, 191, 287, 331, 443, 497, 612, 665
CviQI GTAC 4 cut(s) 343, 488, 589, 672
DdeI CTNAG 2 cut(s) 44, 153
DpnI GATC 3 cut(s) 305, 327, 648
DpnII GATC 3 cut(s) 303, 325, 646
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 614
Eco32I GATATC 1 cut(s) 458
Eco47I GGWCC 3 cut(s) 48, 173, 422
Eco88I CYCGRG 2 cut(s) 250, 690
EcoO109I RGGNCCY 3 cut(s) 48, 610, 611
EcoRII CCWGG 1 cut(s) 438
EcoRV GATATC 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 614
FaeI CATG 1 cut(s) 509
FaqI GGGAC 2 cut(s) 408, 707
FatI CATG 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 159, 444, 666
FokI GGATG 5 cut(s) 351, 365, 519, 635, 672
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 3 cut(s) 159, 444, 666
FspBI CTAG 2 cut(s) 162, 576
GluI GCNGC 3 cut(s) 159, 444, 666
GsaI CCCAGC 1 cut(s) 610
HaeIII GGCC 3 cut(s) 5, 158, 612
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 1 cut(s) 509
HinfI GANTC 5 cut(s) 131, 183, 243, 512, 677
HphI GGTGA 3 cut(s) 238, 391, 398
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 3 cut(s) 154, 455, 527
Hpy188III TCNNGA 2 cut(s) 536, 692
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 362
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 2 cut(s) 357, 670
HpyCH4V TGCA 4 cut(s) 365, 400, 668, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 417
HpyF3I CTNAG 2 cut(s) 44, 153
HpySE526I ACGT 2 cut(s) 357, 670
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 1 cut(s) 509
Kzo9I GATC 3 cut(s) 303, 325, 646
LpnPI CCDG 6 cut(s) 189, 305, 410, 425, 452, 592
Lsp1109I GCAGC 2 cut(s) 430, 652
LweI GCATC 2 cut(s) 541, 613
MaeI CTAG 2 cut(s) 162, 576
MaeII ACGT 2 cut(s) 357, 670
MaeIII GTNAC 2 cut(s) 275, 501
MalI GATC 3 cut(s) 305, 327, 648
MbiI CCGCTC 1 cut(s) 420
MboI GATC 3 cut(s) 303, 325, 646
MboII GAAGA 5 cut(s) 102, 233, 595, 648, 672
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 614
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 209, 232, 597
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 125, 506
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 7 cut(s) 39, 148, 202, 332, 624, 625, 699
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 299, 435
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 440
Mva1269I GAATGC 3 cut(s) 553, 608, 737
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 417
NdeII GATC 3 cut(s) 303, 325, 646
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 4 cut(s) 174, 424, 611, 612
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 275
NspV TTCGAA 1 cut(s) 186
PctI GAATGC 3 cut(s) 553, 608, 737
PfeI GAWTC 3 cut(s) 183, 243, 677
PkrI GCNGC 3 cut(s) 160, 445, 667
PleI GAGTC 2 cut(s) 125, 506
PpsI GAGTC 2 cut(s) 125, 506
Ppu21I YACGTR 1 cut(s) 671
PpuMI RGGWCCY 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 606
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 4 cut(s) 174, 424, 611, 612
PspOMI GGGCCC 1 cut(s) 610
PspPI GGNCC 5 cut(s) 48, 173, 422, 610, 611
PspPPI RGGWCCY 1 cut(s) 48
PsuI RGATCY 1 cut(s) 303
RsaI GTAC 4 cut(s) 344, 489, 590, 673
RsaNI GTAC 4 cut(s) 343, 488, 589, 672
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 2 cut(s) 299, 435
SatI GCNGC 3 cut(s) 159, 444, 666
Sau3AI GATC 3 cut(s) 303, 325, 646
Sau96I GGNCC 5 cut(s) 48, 173, 422, 610, 611
SchI GAGTC 2 cut(s) 125, 506
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 2 cut(s) 541, 613
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 3 cut(s) 48, 173, 422
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 3 cut(s) 209, 232, 597
SsiI CCGC 2 cut(s) 159, 420
SspMI CTAG 2 cut(s) 162, 576
StyD4I CCNGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 2 cut(s) 360, 673
TaqI TCGA 2 cut(s) 186, 324
TasI AATT 3 cut(s) 209, 232, 597
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 3 cut(s) 183, 243, 677
Tru1I TTAA 2 cut(s) 299, 435
Tru9I TTAA 2 cut(s) 299, 435
TscAI CASTG 2 cut(s) 48, 278
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 443, 665
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 242, 560
TspRI CASTG 2 cut(s) 48, 278
VpaK11BI GGWCC 3 cut(s) 48, 173, 422
XapI RAATTY 1 cut(s) 209
XspI CTAG 2 cut(s) 162, 576
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.