Rw6G001350

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009103
Physical Location & Seq
Forward (+)
2004068 .. 2006554
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001350.1

Sequence Viewer

Length: 768 bp
ATGGCCTGTCATTGTGCTTATCACTCATTTCCACCATCACCATTGAGGAGCTTCAGCAACAACAACAACAACAAGACCAGTCTTCTTCTTGCTTCATCAAAAAGACCCAATTCTCTTTTGCTTTCTGCCATCAGCCACAATAGAGAATCTTACAGTACCTTTGTTTCTGAGGCTGCTAGGCTTTTGGGTCCAGCAAGATTTGACGCTTCAAAGCTAAAGGTTGAGTTTATTGGAGAAGAGATGAACAACTATGTCGGAATCATCCCAAGAACTTACATCCTATCTCATTGTGACTTCACAGCTAACTTGACCTTAACCATCTCCAATGTCATCAACCTTGAACAGTTAAAAGGATGGTACAGCAAGGATGATGTTGTTGCAGAGTGGAAAAGGGTGAATGGTGAAATGTGTCTGCATATCCATTGCTATGTGAGCGGTCCCAACCTCTTGCTAGACCTGGCAGCAGAGTTCAGATATCACATATTTTCCAAAGAAATGCCTTTGGTACTGAAAGCTGTGCTACATGGAGACTCACCACTTTTCACAGAGCATCCAGAGCTTCTTGATGCTTTGGTTCGGGTTTATTTTCATTCTAGTTTGAAGAAGTACAATAGAATTGAATGCTGGGGGCCTCTCAAGGATGCTGTAGAGGAAAGACCAAGGGATCGTACTCAAGGAGTAACTGCATCTATTGATCCCCCTCGTCCTCCAGACAAGTGGGCAAGGCCAAAATCCTTATTTCAAGTTCTATTTGCGTTCCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

255

Amino Acids

29.01

Weight (kDa)

8.48

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 66 - 215 3.8e-59 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 435
AciI CCGC 1 cut(s) 435
AclWI GGATC 2 cut(s) 672, 689
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 5 cut(s) 157, 359, 507, 608, 670
AgsI TTSAA 5 cut(s) 210, 341, 601, 620, 743
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 5 cut(s) 51, 214, 302, 515, 559
AluI AGCT 5 cut(s) 51, 214, 302, 515, 559
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 2 cut(s) 672, 689
AoxI GGCC 3 cut(s) 3, 629, 725
ApeKI GCWGC 2 cut(s) 173, 461
AspS9I GGNCC 3 cut(s) 188, 437, 629
AsuHPI GGTGA 4 cut(s) 30, 406, 413, 525
AvaII GGWCC 2 cut(s) 188, 437
BbsI GAAGAC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 160, 473
BccI CCATC 4 cut(s) 43, 137, 326, 348
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 3 cut(s) 177, 452, 594
BfmI CTRYAG 1 cut(s) 645
BisI GCNGC 2 cut(s) 174, 462
BlsI GCNGC 2 cut(s) 175, 463
Bme1390I CCNGG 1 cut(s) 458
Bme18I GGWCC 2 cut(s) 188, 437
BmgT120I GGNCC 3 cut(s) 188, 437, 629
BmiI GGNNCC 3 cut(s) 189, 439, 630
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 4 cut(s) 556, 559, 631, 695
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 620, 657
BsaJI CCNNGG 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 1 cut(s) 659
BseGI GGATG 6 cut(s) 261, 276, 359, 373, 550, 646
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 160, 473
BseYI CCCAGC 1 cut(s) 624
BshFI GGCC 3 cut(s) 5, 631, 727
BslFI GGGAC 1 cut(s) 423
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 626
BsnI GGCC 3 cut(s) 5, 631, 727
Bsp143I GATC 2 cut(s) 664, 694
BspACI CCGC 1 cut(s) 435
BspANI GGCC 3 cut(s) 5, 631, 727
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 189, 439, 630
BspPI GGATC 2 cut(s) 672, 689
BsrBI CCGCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 2 cut(s) 664, 694
BssT1I CCWWGG 1 cut(s) 659
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 155, 345
Bst6I CTCTTC 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 6 cut(s) 261, 276, 359, 373, 550, 646
BstKTI GATC 2 cut(s) 667, 697
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 2 cut(s) 664, 694
BstMWI GCNNNNNNNGC 2 cut(s) 432, 556
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 1 cut(s) 645
BstV1I GCAGC 2 cut(s) 160, 473
BstV2I GAAGAC 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 717
BsuRI GGCC 3 cut(s) 5, 631, 727
BtsCI GGATG 6 cut(s) 261, 276, 359, 373, 550, 646
Cfr13I GGNCC 3 cut(s) 188, 437, 629
CseI GACGC 1 cut(s) 212
Csp6I GTAC 5 cut(s) 156, 358, 506, 607, 669
CviAII CATG 1 cut(s) 524
CviQI GTAC 5 cut(s) 156, 358, 506, 607, 669
DdeI CTNAG 1 cut(s) 168
DpnI GATC 2 cut(s) 666, 696
DpnII GATC 2 cut(s) 664, 694
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 659
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 188, 437
Eco57I CTGAAG 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 456
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 659
FaeI CATG 1 cut(s) 527
FaiI YATR 5 cut(s) 252, 417, 429, 482, 525
FaqI GGGAC 1 cut(s) 423
FatI CATG 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 174, 462
FokI GGATG 6 cut(s) 248, 263, 366, 380, 537, 653
Fsp4HI GCNGC 2 cut(s) 174, 462
FspBI CTAG 3 cut(s) 177, 452, 594
GluI GCNGC 2 cut(s) 174, 462
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 3 cut(s) 5, 631, 727
HgaI GACGC 1 cut(s) 212
Hin1II CATG 1 cut(s) 527
HinfI GANTC 3 cut(s) 146, 258, 530
HphI GGTGA 4 cut(s) 30, 406, 413, 525
Hpy188I TCNGA 3 cut(s) 169, 257, 473
Hpy188III TCNNGA 3 cut(s) 554, 563, 710
HpyCH4III ACNGT 2 cut(s) 155, 345
HpyCH4V TGCA 3 cut(s) 380, 415, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 432, 556
HpyF3I CTNAG 1 cut(s) 168
Hsp92II CATG 1 cut(s) 527
Kzo9I GATC 2 cut(s) 664, 694
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 19, 91, 204, 443, 470, 567, 610, 723
Lsp1109I GCAGC 2 cut(s) 160, 473
LweI GCATC 4 cut(s) 556, 559, 631, 695
MaeI CTAG 3 cut(s) 177, 452, 594
MaeIII GTNAC 2 cut(s) 290, 679
MalI GATC 2 cut(s) 666, 696
MbiI CCGCTC 1 cut(s) 435
MboI GATC 2 cut(s) 664, 694
MboII GAAGA 4 cut(s) 74, 77, 248, 613
MluCI AATT 2 cut(s) 109, 615
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 7 cut(s) 39, 163, 455, 642, 643, 711, 717
MseI TTAA 2 cut(s) 314, 347
MslI CAYNNNNRTG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 626
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 2 cut(s) 432, 556
NdeII GATC 2 cut(s) 664, 694
NlaIII CATG 1 cut(s) 527
NlaIV GGNNCC 3 cut(s) 189, 439, 630
NmuCI GTSAC 1 cut(s) 290
PctI GAATGC 1 cut(s) 626
PfeI GAWTC 2 cut(s) 146, 258
PkrI GCNGC 2 cut(s) 175, 463
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 456
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 3 cut(s) 189, 439, 630
PspPI GGNCC 3 cut(s) 188, 437, 629
RsaI GTAC 5 cut(s) 157, 359, 507, 608, 670
RsaNI GTAC 5 cut(s) 156, 358, 506, 607, 669
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 2 cut(s) 314, 347
SatI GCNGC 2 cut(s) 174, 462
Sau3AI GATC 2 cut(s) 664, 694
Sau96I GGNCC 3 cut(s) 188, 437, 629
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 458
SfaNI GCATC 4 cut(s) 556, 559, 631, 695
SfcI CTRYAG 1 cut(s) 645
SinI GGWCC 2 cut(s) 188, 437
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 2 cut(s) 635, 672
SmoI CTYRAG 2 cut(s) 635, 672
Sse9I AATT 2 cut(s) 109, 615
SsiI CCGC 1 cut(s) 435
SspMI CTAG 3 cut(s) 177, 452, 594
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 659
TaaI ACNGT 2 cut(s) 155, 345
TasI AATT 2 cut(s) 109, 615
TatI WGTACW 1 cut(s) 606
TfiI GAWTC 2 cut(s) 146, 258
Tru1I TTAA 2 cut(s) 314, 347
Tru9I TTAA 2 cut(s) 314, 347
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 2 cut(s) 173, 461
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 3 cut(s) 84, 257, 578
VpaK11BI GGWCC 2 cut(s) 188, 437
XspI CTAG 3 cut(s) 177, 452, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.