RchiOBHm_Chr6g0251541

Protein STAY-GREEN LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009103
Physical Location & Seq
Forward (+)
6806896 .. 6809447
2552 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22549

Sequence Viewer

Length: 762 bp
ATGGCCTGTCATTGTGCTTATCATTCATTTCCACCATCACCATTGAGGAGCTTCAGCAACAACAACAAGACCAGTCTTCTTCTTGCTTCATCAAAAAGACCCAATTCTCTTTTGCTTTCTGCCATCAGCCACAATAGAGAATCTTACAGTACCTTTGTTTCTGAGGCTGCTAGGCTTTTGGGTCCAGCAAGATTTGACGCTTCAAAGCTAAAGGTTGAGTTTATTGGAGAAGAGATGAACAACTATGTCGGAATCATCCCAAGAACTTACATCCTATCTCATTGTGACTTCACAGCTAACTTGACCTTAACCATCTCCAATGTCATCAACCTTGAACAGTTAAAAGGATGGTACAGCAAGGATGATGTTGTTGCAGAGTGGAAAAGGGTGAATGGTGAAATGTGTCTGCATATCCATTGCTATGTGAGCGGTCCCAACCTCTTGCTAGACCTGGCAGCAGAGTTCAGATATCACATATTTTCCAAAGAAATGCCTTTGGTACTGAAAGCTGTGCTACATGGAGACTCACCACTTTTCGGAGAGCATCCAGAGCTACTTGATGCTTTGGTTCGGGTTTATTTTCATTCTAGTTTGAAGAAGTACAATAGAATTGAATGCTGGGGGCCTCTCAAGGATGCTGTAGAGGAAAGACCAAGGGATCGTACTCAAGGAGTAACTGCATCTATTGATCCCCCTCGTCCTCCAGACAAGTGGGCAAGGCCAAAATCCTTATTTCAAGTTCTATTTGCGTTCCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0008150 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
ko00860 ko01110 map00860 map01110
Pfam Domains
Protein Families
Staygreen

Protein Analysis

253

Amino Acids

28.73

Weight (kDa)

8.48

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Staygreen PF12638 64 - 213 1e-58 Staygreen protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 429
AciI CCGC 1 cut(s) 429
AclWI GGATC 2 cut(s) 666, 683
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 5 cut(s) 151, 353, 501, 602, 664
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 5 cut(s) 204, 335, 595, 614, 737
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 5 cut(s) 51, 208, 296, 509, 553
AluI AGCT 5 cut(s) 51, 208, 296, 509, 553
Alw26I GTCTC 1 cut(s) 516
AlwI GGATC 2 cut(s) 666, 683
AoxI GGCC 3 cut(s) 3, 623, 719
ApeKI GCWGC 2 cut(s) 167, 455
AspS9I GGNCC 3 cut(s) 182, 431, 623
AsuHPI GGTGA 4 cut(s) 30, 400, 407, 519
AvaII GGWCC 2 cut(s) 182, 431
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 154, 467
BccI CCATC 4 cut(s) 43, 131, 320, 342
BciT130I CCWGG 1 cut(s) 452
BcoDI GTCTC 1 cut(s) 516
BfaI CTAG 3 cut(s) 171, 446, 588
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 2 cut(s) 168, 456
BlsI GCNGC 2 cut(s) 169, 457
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 2 cut(s) 182, 431
BmgT120I GGNCC 3 cut(s) 182, 431, 623
BmiI GGNNCC 3 cut(s) 183, 433, 624
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 4 cut(s) 550, 553, 625, 689
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 687
BpuEI CTTGAG 2 cut(s) 614, 651
BsaJI CCNNGG 1 cut(s) 653
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 6 cut(s) 255, 270, 353, 367, 544, 640
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 154, 467
BseYI CCCAGC 1 cut(s) 618
BshFI GGCC 3 cut(s) 5, 625, 721
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 536
BsmAI GTCTC 1 cut(s) 516
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 3 cut(s) 5, 625, 721
Bsp143I GATC 2 cut(s) 658, 688
BspACI CCGC 1 cut(s) 429
BspANI GGCC 3 cut(s) 5, 625, 721
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 183, 433, 624
BspPI GGATC 2 cut(s) 666, 683
BsrBI CCGCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 2 cut(s) 658, 688
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 2 cut(s) 149, 339
Bst6I CTCTTC 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 6 cut(s) 255, 270, 353, 367, 544, 640
BstKTI GATC 2 cut(s) 661, 691
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 2 cut(s) 658, 688
BstMWI GCNNNNNNNGC 2 cut(s) 426, 550
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 639
BstV1I GCAGC 2 cut(s) 154, 467
BstV2I GAAGAC 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 5, 625, 721
BtsCI GGATG 6 cut(s) 255, 270, 353, 367, 544, 640
Cfr13I GGNCC 3 cut(s) 182, 431, 623
CseI GACGC 1 cut(s) 206
Csp6I GTAC 5 cut(s) 150, 352, 500, 601, 663
CviAII CATG 1 cut(s) 518
CviQI GTAC 5 cut(s) 150, 352, 500, 601, 663
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 660, 690
DpnII GATC 2 cut(s) 658, 688
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 653
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 182, 431
Eco57I CTGAAG 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 450
EcoRV GATATC 1 cut(s) 470
EcoT14I CCWWGG 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 1 cut(s) 521
FaiI YATR 5 cut(s) 246, 411, 423, 476, 519
FaqI GGGAC 1 cut(s) 417
FatI CATG 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 168, 456
FokI GGATG 6 cut(s) 242, 257, 360, 374, 531, 647
Fsp4HI GCNGC 2 cut(s) 168, 456
FspBI CTAG 3 cut(s) 171, 446, 588
GluI GCNGC 2 cut(s) 168, 456
GsaI CCCAGC 1 cut(s) 622
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 3 cut(s) 5, 625, 721
HgaI GACGC 1 cut(s) 206
Hin1II CATG 1 cut(s) 521
HinfI GANTC 3 cut(s) 140, 252, 524
HphI GGTGA 4 cut(s) 30, 400, 407, 519
Hpy188I TCNGA 4 cut(s) 163, 251, 467, 539
Hpy188III TCNNGA 2 cut(s) 548, 704
HpyCH4III ACNGT 2 cut(s) 149, 339
HpyCH4V TGCA 3 cut(s) 374, 409, 680
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 550
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 1 cut(s) 521
Kzo9I GATC 2 cut(s) 658, 688
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 19, 85, 198, 437, 464, 561, 604, 717
Lsp1109I GCAGC 2 cut(s) 154, 467
LweI GCATC 4 cut(s) 550, 553, 625, 689
MaeI CTAG 3 cut(s) 171, 446, 588
MaeIII GTNAC 2 cut(s) 284, 673
MalI GATC 2 cut(s) 660, 690
MbiI CCGCTC 1 cut(s) 429
MboI GATC 2 cut(s) 658, 688
MboII GAAGA 4 cut(s) 68, 71, 242, 607
MluCI AATT 2 cut(s) 103, 609
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 7 cut(s) 39, 157, 449, 636, 637, 705, 711
MseI TTAA 2 cut(s) 308, 341
MslI CAYNNNNRTG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 426, 550
NdeII GATC 2 cut(s) 658, 688
NlaIII CATG 1 cut(s) 521
NlaIV GGNNCC 3 cut(s) 183, 433, 624
NmuCI GTSAC 1 cut(s) 284
PctI GAATGC 1 cut(s) 620
PfeI GAWTC 2 cut(s) 140, 252
PkrI GCNGC 2 cut(s) 169, 457
PleI GAGTC 1 cut(s) 518
PpsI GAGTC 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 618
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 183, 433, 624
PspPI GGNCC 3 cut(s) 182, 431, 623
RsaI GTAC 5 cut(s) 151, 353, 501, 602, 664
RsaNI GTAC 5 cut(s) 150, 352, 500, 601, 663
RseI CAYNNNNRTG 1 cut(s) 420
SaqAI TTAA 2 cut(s) 308, 341
SatI GCNGC 2 cut(s) 168, 456
Sau3AI GATC 2 cut(s) 658, 688
Sau96I GGNCC 3 cut(s) 182, 431, 623
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 1 cut(s) 452
SfaNI GCATC 4 cut(s) 550, 553, 625, 689
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 2 cut(s) 182, 431
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 2 cut(s) 629, 666
SmoI CTYRAG 2 cut(s) 629, 666
Sse9I AATT 2 cut(s) 103, 609
SsiI CCGC 1 cut(s) 429
SspMI CTAG 3 cut(s) 171, 446, 588
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 2 cut(s) 149, 339
TasI AATT 2 cut(s) 103, 609
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 2 cut(s) 140, 252
Tru1I TTAA 2 cut(s) 308, 341
Tru9I TTAA 2 cut(s) 308, 341
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 2 cut(s) 167, 455
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 15, 78, 251, 572
VpaK11BI GGWCC 2 cut(s) 182, 431
XspI CTAG 3 cut(s) 171, 446, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.