FvH4_2g03350

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012175
Physical Location & Seq
Forward (+)
2645437 .. 2646389
953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g03350.t1

Sequence Viewer

Length: 474 bp
ATGGGCAAAGAGAAGCAGTATTGGCTTCTGAAAACAGAGCCAGGCGAGTGGTCATGGGAGGACCAAGCAGCCAACAACGGCATAACCAAGTGGGATGGAGTCAAGAACAAGCAGGCACAAAAGTACCTCAAGTCCATGAACCTCGATGACCTCTGCTTCTTCTACCACTCCGGCGCTAAAGCCCGCCGCGTAGTCGGCGTCGTGAAGGTCGTGCGCGAATGGTATTCGGAAGGCGAAAACGGTGACGGTGCGGTTGACGTGAAGGCGATTGGGGAGATGAGGAGGCCGATTGACTTGAAGGAGATGAAGGGCGAGGAGGGTCTTAAAGGGTTTGCTCTGTTTCGGCAGCCGAGGCTTTCGGTTGTGCCGGTTTCGGAGGATGTGTGGTTGAAAGTTTGTGATTTGGGAGGTGGGTTTGTAGGGGATGGTGTTGTTGTGGAGGATGATGTATCTGGTGATGAAGTAGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

158

Amino Acids

17.57

Weight (kDa)

4.88

Isoelectric Point (pI)

27.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 134 3.6e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 189, 216
AciI CCGC 3 cut(s) 184, 187, 251
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 2 cut(s) 298, 391
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 40
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 68, 346
AspLEI GCGC 2 cut(s) 176, 216
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 254, 467
AvaII GGWCC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 107, 140
BbvI GCAGC 2 cut(s) 80, 358
BccI CCATC 2 cut(s) 89, 419
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 3 cut(s) 69, 187, 347
BlsI GCNGC 3 cut(s) 70, 188, 348
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bse118I RCCGGY 1 cut(s) 367
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 4 cut(s) 100, 385, 430, 448
BseRI GAGGAG 2 cut(s) 295, 329
BseXI GCAGC 2 cut(s) 80, 358
Bsh1236I CGCG 2 cut(s) 189, 216
BshFI GGCC 1 cut(s) 286
BsiSI CCGG 2 cut(s) 171, 368
BsnI GGCC 1 cut(s) 286
BspACI CCGC 3 cut(s) 184, 187, 251
BspANI GGCC 1 cut(s) 286
BspFNI CGCG 2 cut(s) 189, 216
BsrFI RCCGGY 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 242, 248
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 114, 184
BstF5I GGATG 4 cut(s) 100, 385, 430, 448
BstFNI CGCG 2 cut(s) 189, 216
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 2 cut(s) 176, 216
BstMWI GCNNNNNNNGC 3 cut(s) 22, 195, 352
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 2 cut(s) 189, 216
BstV1I GCAGC 2 cut(s) 80, 358
BstXI CCANNNNNNTGG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 286
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 4 cut(s) 100, 385, 430, 448
Cac8I GCNNGC 2 cut(s) 114, 184
CfoI GCGC 2 cut(s) 176, 216
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 61
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 124
CviAII CATG 2 cut(s) 54, 136
CviJI RGCY 7 cut(s) 25, 40, 71, 182, 286, 349, 355
CviKI_1 RGCY 7 cut(s) 25, 40, 71, 182, 286, 349, 355
CviQI GTAC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 57, 139
FaiI YATR 3 cut(s) 55, 83, 137
FatI CATG 2 cut(s) 53, 135
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 3 cut(s) 69, 187, 347
FokI GGATG 4 cut(s) 107, 392, 437, 455
Fsp4HI GCNGC 3 cut(s) 69, 187, 347
GlaI GCGC 2 cut(s) 175, 215
GluI GCNGC 3 cut(s) 69, 187, 347
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 1 cut(s) 286
HapII CCGG 2 cut(s) 171, 368
HgaI GACGC 1 cut(s) 187
HhaI GCGC 2 cut(s) 176, 216
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 57, 139
Hin6I GCGC 2 cut(s) 174, 214
HinP1I GCGC 2 cut(s) 174, 214
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 1 cut(s) 99
HpaII CCGG 2 cut(s) 171, 368
HphI GGTGA 2 cut(s) 254, 467
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 3 cut(s) 30, 229, 376
Hpy188III TCNNGA 2 cut(s) 103, 202
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 5 cut(s) 199, 224, 256, 292, 301
HpyCH4III ACNGT 2 cut(s) 242, 248
HpyCH4IV ACGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 195, 352
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 57, 139
HspAI GCGC 2 cut(s) 174, 214
LpnPI CCDG 6 cut(s) 27, 54, 98, 184, 381, 438
Lsp1109I GCAGC 2 cut(s) 80, 358
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 242
MboII GAAGA 1 cut(s) 151
MlyI GAGTC 1 cut(s) 108
MseI TTAA 1 cut(s) 324
MspI CCGG 2 cut(s) 171, 368
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
MvnI CGCG 2 cut(s) 189, 216
MwoI GCNNNNNNNGC 3 cut(s) 22, 195, 352
NlaIII CATG 2 cut(s) 57, 139
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PkrI GCNGC 3 cut(s) 70, 188, 348
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspPI GGNCC 1 cut(s) 61
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 3 cut(s) 69, 187, 347
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 42
SetI ASST 6 cut(s) 129, 144, 153, 210, 261, 412
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
SsiI CCGC 3 cut(s) 184, 187, 251
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 242, 248
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 144
TauI GCSGC 1 cut(s) 189
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 2 cut(s) 68, 346
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 152, 320, 474
VpaK11BI GGWCC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.