Rorug05G0531700

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012175
Physical Location & Seq
Forward (+)
72010566 .. 72014470
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0531700.1

Sequence Viewer

Length: 1497 bp
ATGGACTGCCCTCATGCTACTGAGATCTGGACTCAGGCTGGTGTGCGTTGGTCGGGCGGGAATGTTGATTCTATCCAAGCGTGGGTTATGGCTTTAATACCTACTTTATCCAAAGAAGAATTGAATAAGGTGCTGATGTTGTTGTGGGGTATATGGAAGAATAGAAATGCTCAAGTTTGGGAACAGAAAGGTAAGCATGCTTCTGACACATTGTTGTTGACGTTGGGATGGTATGAAGAGTACAAGAAGTATCATGTGGTTTCAAGAAGACAGAGGCCCCCACCTTCACATTGGATTGTTCCAGCTCCAGGCCAGTACAAAATGAATTGTGATGGAGCTTATCAAGCATCTTCCAGACGTGGTGGAGGAGGCTATGTCATGCGTGACAGTGCAGGCAGCTTTATAGCGGGACAGACGAAGCCATATGGCTTGCTTACATCACCTTTTCATGCTGAATTGCTAGCTCTTAGGGACGGATCATCGAGCCATTCGTGGCATCCAGTCATGACCACGGATACTAATGTTCTAAGCTATTGGAATAACTGGAGGTTCTTGATCTGTGCAATATTTATATTGAGTAGTATCGTGCTAGCCGCAGTTCTTATATGGAAATATGAAGGCTGCAAAAAATTCAGAAGGGAAAGAAGAGAAAATCAGCGCGAAACAGTAGGATCTCTATATGAGGATGAAGCCTGGAGGACATCTCTCAAAGTCATTCATCCCGCTTGGCTTCTTGCTTACAGATTAATTGCTTTCGTTGTGCTTCTATCCTTGATAGTTACCAATATTGTTCTTGATGGAGTTGGCATACTTTACTTCTACACTCAGTGGACATTTTGTTTGGTTACAATCTATTTTGGGCTTGGATCTTCACTCTCCATTTATGGATGCTACAAATACCGCAGTAGAGTTGGTGAGGATGCAGCTGATCATGTGAGAGATGAAGAGCGAGGTACTTATGTTGCTCCAACACTTGGGGAAAATGCAGATACATCCAACACATATAAAAGTTTTACTGATGAAGAATGTCCCTCTCGCAAAGGCGCTGGTGTATATGTTTATATCTTTCAAGTCATTTATCAGATGTGCGGAGGTGCTGTGGTGCTCACAGATGTTATATTTTGGCTCGTTTTGTATCCATTTCTAACAAGCAAAGATTACAAACTGAACTTTATGATGGTCAGTATGCACTCATTCAATGTTGTTTTCCTCCTTGGTGAGACAATATTGAATAGCTTGCGGTTTCCATTGTTTCGGATCTCATATTTTGTGCTATGGACGGGAATATTCGTGATTTTCCAATGGATAATCCATGCTTGTAAAAACATGTGGTGGCCTTATCCATTTCTTGACTTGTCATCCTCATATGCACCCTTATGGTATCTTGGGGTCGGAGTGGCGCATATCCCATGCTACGGCGCCTATGCTTTAATTATTAGGATGAAGCAGCTGTGGTTCTCACGATCATTCCCGGAGTCTTTTCAGGTTGAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

498

Amino Acids

57.36

Weight (kDa)

8.81

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1418
AccB7I CCANNNNNTGG 1 cut(s) 974
AccII CGCG 1 cut(s) 660
AciI CCGC 7 cut(s) 57, 407, 594, 723, 901, 1089, 1240
AclWI GGATC 4 cut(s) 484, 679, 874, 1265
AcsI RAATTY 1 cut(s) 629
AcyI GRCGYC 1 cut(s) 1419
AdeI CACNNNGTG 1 cut(s) 828
AfaI GTAC 3 cut(s) 242, 317, 955
AfiI CCNNNNNNNGG 4 cut(s) 82, 308, 974, 1415
AflIII ACRYGT 1 cut(s) 1326
AgsI TTSAA 5 cut(s) 124, 264, 1070, 1198, 1231
AjiI CACGTC 1 cut(s) 359
AjnI CCWGG 2 cut(s) 307, 692
AluBI AGCT 8 cut(s) 305, 338, 399, 464, 531, 926, 1236, 1450
AluI AGCT 8 cut(s) 305, 338, 399, 464, 531, 926, 1236, 1450
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 1 cut(s) 1214
AlwI GGATC 4 cut(s) 484, 679, 874, 1265
AoxI GGCC 3 cut(s) 275, 310, 1334
ApeKI GCWGC 4 cut(s) 396, 621, 923, 1447
ApoI RAATTY 1 cut(s) 629
ArsI GACNNNNNNTTYG 2 cut(s) 823, 855
AseI ATTAAT 1 cut(s) 746
AspLEI GCGC 4 cut(s) 660, 1046, 1402, 1421
AspS9I GGNCC 1 cut(s) 276
AsuC2I CCSGG 1 cut(s) 1472
AsuHPI GGTGA 3 cut(s) 432, 926, 1229
AsuNHI GCTAGC 2 cut(s) 460, 589
BaeI ACNNNNGTAYC 4 cut(s) 507, 540, 945, 978
BanI GGYRCC 1 cut(s) 1418
BarI GAAGNNNNNNTAC 2 cut(s) 184, 216
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 4 cut(s) 408, 608, 935, 1459
BccI CCATC 4 cut(s) 222, 326, 791, 1171
BceAI ACGGC 1 cut(s) 1432
BciT130I CCWGG 2 cut(s) 309, 694
BciVI GTATCC 2 cut(s) 508, 1146
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 1 cut(s) 1472
BcoDI GTCTC 1 cut(s) 1214
BfaI CTAG 2 cut(s) 461, 590
BfoI RGCGCY 2 cut(s) 1047, 1422
BfuI GTATCC 2 cut(s) 508, 1146
BglII AGATCT 1 cut(s) 24
BisI GCNGC 5 cut(s) 397, 594, 622, 924, 1448
BlsI GCNGC 5 cut(s) 398, 595, 623, 925, 1449
Bme1390I CCNGG 3 cut(s) 309, 694, 1472
BmgBI CACGTC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 278, 1420
BmrFI CCNGG 3 cut(s) 309, 694, 1472
BmsI GCATC 4 cut(s) 356, 505, 878, 910
BmtI GCTAGC 2 cut(s) 464, 593
BpiI GAAGAC 1 cut(s) 274
BplI GAGNNNNNCTC 2 cut(s) 688, 720
BpmI CTGGAG 3 cut(s) 291, 565, 715
BpuEI CTTGAG 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 1472
BsaHI GRCGYC 1 cut(s) 1419
BsaJI CCNNGG 2 cut(s) 510, 1213
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 1083, 1113
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 308, 974, 1415
Bse1I ACTGG 3 cut(s) 313, 500, 548
BseBI CCWGG 2 cut(s) 309, 694
BseDI CCNNGG 2 cut(s) 510, 1213
BseGI GGATG 9 cut(s) 233, 496, 691, 718, 893, 925, 992, 1358, 1446
BseLI CCNNNNNNNGG 4 cut(s) 82, 308, 974, 1415
BseMII CTCAG 3 cut(s) 12, 47, 839
BseNI ACTGG 3 cut(s) 313, 500, 548
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 4 cut(s) 408, 608, 935, 1459
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 3 cut(s) 277, 312, 1336
BshNI GGYRCC 1 cut(s) 1418
BsiHKAI GWGCWC 1 cut(s) 1107
BsiSI CCGG 1 cut(s) 1472
BslFI GGGAC 3 cut(s) 423, 485, 1014
BslI CCNNNNNNNGG 4 cut(s) 82, 308, 974, 1415
BsmAI GTCTC 1 cut(s) 1214
BsmFI GGGAC 3 cut(s) 423, 485, 1014
BsnI GGCC 3 cut(s) 277, 312, 1336
Bsp1286I GDGCHC 1 cut(s) 1107
Bsp143I GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
BspACI CCGC 7 cut(s) 57, 407, 594, 723, 901, 1089, 1240
BspANI GGCC 3 cut(s) 277, 312, 1336
BspCNI CTCAG 3 cut(s) 13, 46, 838
BspFNI CGCG 1 cut(s) 660
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 278, 1420
BspOI GCTAGC 2 cut(s) 464, 593
BspPI GGATC 4 cut(s) 484, 679, 874, 1265
BspQI GCTCTTC 1 cut(s) 939
BspT107I GGYRCC 1 cut(s) 1418
BsrI ACTGG 3 cut(s) 313, 500, 548
BssECI CCNNGG 2 cut(s) 510, 1213
BssMI GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
BssNI GRCGYC 1 cut(s) 1419
BssT1I CCWWGG 1 cut(s) 1213
Bst2UI CCWGG 2 cut(s) 309, 694
Bst4CI ACNGT 2 cut(s) 389, 667
Bst6I CTCTTC 3 cut(s) 231, 640, 939
BstACI GRCGYC 1 cut(s) 1419
BstC8I GCNNGC 6 cut(s) 198, 394, 431, 462, 591, 1238
BstDEI CTNAG 5 cut(s) 21, 33, 467, 527, 825
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 9 cut(s) 233, 496, 691, 718, 893, 925, 992, 1358, 1446
BstFNI CGCG 1 cut(s) 660
BstH2I RGCGCY 2 cut(s) 1047, 1422
BstHHI GCGC 4 cut(s) 660, 1046, 1402, 1421
BstKTI GATC 8 cut(s) 27, 479, 558, 674, 869, 931, 1260, 1466
BstMAI GTCTC 1 cut(s) 1214
BstMBI GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 2 cut(s) 309, 694
BstNSI RCATGY 2 cut(s) 200, 1330
BstSCI CCNGG 3 cut(s) 307, 692, 1470
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 4 cut(s) 408, 608, 935, 1459
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 4 cut(s) 24, 671, 866, 1257
BstYI RGATCY 4 cut(s) 24, 671, 866, 1257
BsuI GTATCC 2 cut(s) 508, 1146
BsuRI GGCC 3 cut(s) 277, 312, 1336
BtgI CCRYGG 1 cut(s) 510
BtrI CACGTC 1 cut(s) 359
BtsCI GGATG 9 cut(s) 233, 496, 691, 718, 893, 925, 992, 1358, 1446
BtsIMutI CAGTG 2 cut(s) 394, 833
Cac8I GCNNGC 6 cut(s) 198, 394, 431, 462, 591, 1238
CciI TCATGA 1 cut(s) 504
CfoI GCGC 4 cut(s) 660, 1046, 1402, 1421
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 3 cut(s) 241, 316, 954
CviQI GTAC 3 cut(s) 241, 316, 954
DdeI CTNAG 5 cut(s) 21, 33, 467, 527, 825
DinI GGCGCC 1 cut(s) 1420
DpnI GATC 8 cut(s) 26, 478, 557, 673, 868, 930, 1259, 1465
DpnII GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
DraIII CACNNNGTG 1 cut(s) 828
Eam1104I CTCTTC 3 cut(s) 231, 640, 939
EarI CTCTTC 3 cut(s) 231, 640, 939
Eco130I CCWWGG 1 cut(s) 1213
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 307, 692
EcoT14I CCWWGG 1 cut(s) 1213
EgeI GGCGCC 1 cut(s) 1420
EheI GGCGCC 1 cut(s) 1420
ErhI CCWWGG 1 cut(s) 1213
FaqI GGGAC 3 cut(s) 423, 485, 1014
FauI CCCGC 3 cut(s) 50, 400, 730
FauNDI CATATG 2 cut(s) 424, 1366
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 5 cut(s) 397, 594, 622, 924, 1448
FokI GGATG 9 cut(s) 240, 483, 698, 705, 900, 932, 979, 1345, 1453
Fsp4HI GCNGC 5 cut(s) 397, 594, 622, 924, 1448
FspBI CTAG 2 cut(s) 461, 590
GlaI GCGC 4 cut(s) 659, 1045, 1401, 1420
GluI GCNGC 5 cut(s) 397, 594, 622, 924, 1448
GsuI CTGGAG 3 cut(s) 291, 565, 715
HaeII RGCGCY 2 cut(s) 1047, 1422
HaeIII GGCC 3 cut(s) 277, 312, 1336
HapII CCGG 1 cut(s) 1472
HhaI GCGC 4 cut(s) 660, 1046, 1402, 1421
Hin1I GRCGYC 1 cut(s) 1419
Hin6I GCGC 4 cut(s) 658, 1044, 1400, 1419
HinP1I GCGC 4 cut(s) 658, 1044, 1400, 1419
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 3 cut(s) 31, 68, 1475
HpaII CCGG 1 cut(s) 1472
HphI GGTGA 3 cut(s) 432, 926, 1229
Hpy166II GTNNAC 2 cut(s) 219, 831
Hpy188I TCNGA 5 cut(s) 205, 635, 1083, 1257, 1394
Hpy188III TCNNGA 9 cut(s) 28, 264, 354, 505, 553, 794, 1291, 1349, 1461
Hpy8I GTNNAC 2 cut(s) 219, 831
HpyAV CCTTC 3 cut(s) 294, 611, 630
HpyCH4III ACNGT 2 cut(s) 389, 667
HpyCH4IV ACGT 2 cut(s) 221, 358
HpyCH4V TGCA 7 cut(s) 392, 563, 624, 923, 986, 1189, 1370
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 5 cut(s) 21, 33, 467, 527, 825
HpySE526I ACGT 2 cut(s) 221, 358
Hsp92I GRCGYC 1 cut(s) 1419
HspAI GCGC 4 cut(s) 658, 1044, 1400, 1419
KasI GGCGCC 1 cut(s) 1418
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
LguI GCTCTTC 1 cut(s) 939
LmnI GCTCC 3 cut(s) 310, 335, 970
Lsp1109I GCAGC 4 cut(s) 408, 608, 935, 1459
LweI GCATC 4 cut(s) 356, 505, 878, 910
MaeI CTAG 2 cut(s) 461, 590
MaeII ACGT 2 cut(s) 221, 358
MaeIII GTNAC 3 cut(s) 383, 778, 844
MalI GATC 8 cut(s) 26, 478, 557, 673, 868, 930, 1259, 1465
MboI GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
MboII GAAGA 9 cut(s) 128, 169, 248, 279, 342, 657, 861, 956, 1034
MflI RGATCY 4 cut(s) 24, 671, 866, 1257
MhlI GDGCHC 1 cut(s) 1107
MluCI AATT 6 cut(s) 119, 325, 455, 629, 747, 1431
Mly113I GGCGCC 1 cut(s) 1419
MlyI GAGTC 2 cut(s) 25, 1484
MmeI TCCRAC 3 cut(s) 992, 1020, 1372
MseI TTAA 3 cut(s) 95, 746, 1430
MslI CAYNNNNRTG 1 cut(s) 1375
MspA1I CMGCKG 2 cut(s) 926, 1450
MspI CCGG 1 cut(s) 1472
MspR9I CCNGG 3 cut(s) 309, 694, 1472
MvaI CCWGG 2 cut(s) 309, 694
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 1 cut(s) 344
NarI GGCGCC 1 cut(s) 1419
NciI CCSGG 1 cut(s) 1472
NdeI CATATG 2 cut(s) 424, 1366
NdeII GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
NheI GCTAGC 2 cut(s) 460, 589
NlaIV GGNNCC 2 cut(s) 278, 1420
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 2 cut(s) 200, 1330
PaeI GCATGC 1 cut(s) 200
PagI TCATGA 1 cut(s) 504
PciI ACATGT 1 cut(s) 1326
PciSI GCTCTTC 1 cut(s) 939
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 974
PfoI TCCNGGA 1 cut(s) 1470
PkrI GCNGC 5 cut(s) 398, 595, 623, 925, 1449
PleI GAGTC 2 cut(s) 25, 1483
PluTI GGCGCC 1 cut(s) 1422
PpsI GAGTC 2 cut(s) 25, 1483
PscI ACATGT 1 cut(s) 1326
PshBI ATTAAT 1 cut(s) 746
Psp6I CCWGG 2 cut(s) 307, 692
PspGI CCWGG 2 cut(s) 307, 692
PspN4I GGNNCC 2 cut(s) 278, 1420
PspPI GGNCC 1 cut(s) 276
PsuI RGATCY 4 cut(s) 24, 671, 866, 1257
PvuII CAGCTG 2 cut(s) 926, 1450
RsaI GTAC 3 cut(s) 242, 317, 955
RsaNI GTAC 3 cut(s) 241, 316, 954
RseI CAYNNNNRTG 1 cut(s) 1375
SapI GCTCTTC 1 cut(s) 939
SaqAI TTAA 3 cut(s) 95, 746, 1430
SatI GCNGC 5 cut(s) 397, 594, 622, 924, 1448
Sau3AI GATC 8 cut(s) 24, 476, 555, 671, 866, 928, 1257, 1463
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 2 cut(s) 25, 1484
ScrFI CCNGG 3 cut(s) 309, 694, 1472
SduI GDGCHC 1 cut(s) 1107
SfaNI GCATC 4 cut(s) 356, 505, 878, 910
SfoI GGCGCC 1 cut(s) 1420
SmiMI CAYNNNNRTG 1 cut(s) 1375
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
SphI GCATGC 1 cut(s) 200
Sse9I AATT 6 cut(s) 119, 325, 455, 629, 747, 1431
SsiI CCGC 7 cut(s) 57, 407, 594, 723, 901, 1089, 1240
SspDI GGCGCC 1 cut(s) 1418
SspI AATATT 4 cut(s) 567, 787, 1227, 1287
SspMI CTAG 2 cut(s) 461, 590
StyD4I CCNGG 3 cut(s) 307, 692, 1470
StyI CCWWGG 1 cut(s) 1213
TaaI ACNGT 2 cut(s) 389, 667
TaiI ACGT 2 cut(s) 224, 361
TaqI TCGA 1 cut(s) 482
TasI AATT 6 cut(s) 119, 325, 455, 629, 747, 1431
TatI WGTACW 2 cut(s) 240, 315
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 95, 746, 1430
Tru9I TTAA 3 cut(s) 95, 746, 1430
TscAI CASTG 2 cut(s) 394, 833
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 4 cut(s) 396, 621, 923, 1447
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 9 cut(s) 249, 338, 437, 630, 702, 707, 957, 1035, 1457
TspGWI ACGGA 2 cut(s) 489, 527
TspRI CASTG 2 cut(s) 394, 833
Van91I CCANNNNNTGG 1 cut(s) 974
VspI ATTAAT 1 cut(s) 746
XapI RAATTY 1 cut(s) 629
XceI RCATGY 2 cut(s) 200, 1330
XcmI CCANNNNNNNNNTGG 1 cut(s) 288
XspI CTAG 2 cut(s) 461, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.