Rroxscaffold_7G00213250

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012175
Physical Location & Seq
Reverse (-)
63695063 .. 63695539
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00213250.1

Sequence Viewer

Length: 477 bp
ATGGGCAAAGAGAAGCAGTATTGGCTTCTGAAAACAGAGCCAGGAGAGTGGTCGTGGGAGGACCAAGCAGCCAATGAGGGCATCACCAAGTGGGATGGAGTCAAGAACAAGCAGGCCCAGAAGTACCTCAAGTCCATGAACCTCGATGACCTCTGTTTCTTCTACCACTCCGGCGCCAAGGCCCGCCGCATAGTCGGCGTCGTGACGGTCGTGCGTGAATGGTATTCGGAAGGCGAAGGTGGTGACGGTGCGGTTGATGTGAAGGCAGTTGGGGAGATGAGGAGGCCGGTTGACTTGAAGGAGATGAAGGGGGAGGAGGGTCTCAAGGGTTTTGCTCTGTTTCGTCAGCCGAGGCTGTCGGTTGTGCCGGTTTCGGAGGATGTTTGGTTGAAAGTTTGTGATTTGGGAGGTGGGTTTGAAGGTGATGGTGTTGTTGTGGAAGATGATGAATCTGGTGATGAAGGAGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

158

Amino Acids

17.62

Weight (kDa)

4.6

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 134 2.7e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 3 cut(s) 184, 187, 251
AcyI GRCGYC 2 cut(s) 174, 198
AdeI CACNNNGTG 1 cut(s) 90
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 3 cut(s) 298, 391, 419
AjnI CCWGG 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 326
AoxI GGCC 3 cut(s) 114, 180, 284
ApeKI GCWGC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 61, 115, 181
AsuHPI GGTGA 4 cut(s) 76, 254, 434, 467
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 89, 419
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 2 cut(s) 69, 187
BlsI GCNGC 2 cut(s) 70, 188
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 61, 115, 181
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 113, 308
BsaHI GRCGYC 2 cut(s) 174, 198
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 177, 350
Bse118I RCCGGY 2 cut(s) 286, 367
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 177, 350
BseGI GGATG 2 cut(s) 100, 385
BseRI GAGGAG 2 cut(s) 295, 329
BseXI GCAGC 1 cut(s) 80
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 116, 182, 286
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 3 cut(s) 171, 287, 368
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 116, 182, 286
Bso31I GGTCTC 1 cut(s) 326
BspACI CCGC 3 cut(s) 184, 187, 251
BspANI GGCC 3 cut(s) 116, 182, 286
BspLI GGNNCC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 2 cut(s) 286, 367
BssAI RCCGGY 2 cut(s) 286, 367
BssECI CCNNGG 2 cut(s) 177, 350
BssNI GRCGYC 2 cut(s) 174, 198
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 208, 248
BstACI GRCGYC 2 cut(s) 174, 198
BstC8I GCNNGC 2 cut(s) 114, 184
BstF5I GGATG 2 cut(s) 100, 385
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 326
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 22, 195
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 116, 182, 286
BtsCI GGATG 2 cut(s) 100, 385
Cac8I GCNNGC 2 cut(s) 114, 184
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 286, 367
Cfr13I GGNCC 3 cut(s) 61, 115, 181
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 136
CviJI RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviKI_1 RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviQI GTAC 1 cut(s) 124
DinI GGCGCC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 177
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 139
FaiI YATR 2 cut(s) 137, 191
FatI CATG 1 cut(s) 135
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 2 cut(s) 69, 187
FokI GGATG 2 cut(s) 107, 392
Fsp4HI GCNGC 2 cut(s) 69, 187
GlaI GCGC 1 cut(s) 175
GluI GCNGC 2 cut(s) 69, 187
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 3 cut(s) 116, 182, 286
HapII CCGG 3 cut(s) 171, 287, 368
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 2 cut(s) 174, 198
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 2 cut(s) 99, 449
HpaII CCGG 3 cut(s) 171, 287, 368
HphI GGTGA 4 cut(s) 76, 254, 434, 467
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 3 cut(s) 30, 229, 376
Hpy188III TCNNGA 2 cut(s) 103, 202
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 7 cut(s) 224, 230, 256, 292, 301, 413, 455
HpyCH4III ACNGT 2 cut(s) 208, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 195
Hsp92I GRCGYC 2 cut(s) 174, 198
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 1 cut(s) 174
KasI GGCGCC 1 cut(s) 173
LpnPI CCDG 8 cut(s) 27, 54, 98, 131, 184, 300, 381, 438
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 202, 242
MboII GAAGA 2 cut(s) 151, 452
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 108
MspI CCGG 3 cut(s) 171, 287, 368
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 2 cut(s) 22, 195
NarI GGCGCC 1 cut(s) 174
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 202, 242
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 2 cut(s) 70, 188
PleI GAGTC 1 cut(s) 107
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 3 cut(s) 61, 115, 181
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 2 cut(s) 69, 187
Sau96I GGNCC 3 cut(s) 61, 115, 181
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 42
SetI ASST 6 cut(s) 129, 144, 153, 241, 412, 424
SfaNI GCATC 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 175
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 128, 323
SmoI CTYRAG 2 cut(s) 128, 323
SsiI CCGC 3 cut(s) 184, 187, 251
SspDI GGCGCC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 2 cut(s) 208, 248
TaqI TCGA 1 cut(s) 144
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 449
TseFI GTSAC 2 cut(s) 202, 242
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 2 cut(s) 202, 242
TspDTI ATGAA 4 cut(s) 152, 320, 462, 474
VpaK11BI GGWCC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.