MD05G1105400.v1.1

EVE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012175
Physical Location & Seq
Reverse (-)
21867458 .. 21867703
246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1105400.v1.1.491

Sequence Viewer

Length: 246 bp
ATGGCTCCAGAGAAACAATACTGGCTTCTCAAGTCCATGAAGCTCAGCGACCTCTGCTGCTTCTACCACTCGGGCGCTAAGGCCTGCCGCGTAATCCGCGTCTTCACCATCGTGCGGGAATGGTATTTAGAAAAAGGGGATGATGGGGTGGTTGATGTGAAGGTGGCTGGGGAGATGAGGAAGCCGATGGACTTAAAGGAGATGAATGGGGAGGAGGGGCTTAAGGGGTTTGCTTTGTTTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

82

Amino Acids

9.36

Weight (kDa)

8.47

Isoelectric Point (pI)

13.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 9 - 80 1.8e-07 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 90, 99
AciI CCGC 3 cut(s) 88, 97, 115
AfiI CCNNNNNNNGG 1 cut(s) 114
AflII CTTAAG 1 cut(s) 221
AleI CACNNNNGTG 1 cut(s) 110
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 44
BccI CCATC 3 cut(s) 116, 137, 181
BfoI RGCGCY 1 cut(s) 78
BfrI CTTAAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 58, 88
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 2 cut(s) 59, 89
BmeT110I CYCGRG 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 14
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 44
BseYI CCCAGC 1 cut(s) 167
Bsh1236I CGCG 2 cut(s) 90, 99
BshFI GGCC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 114
BsnI GGCC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 70
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 3 cut(s) 88, 97, 115
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 57
BspFNI CGCG 2 cut(s) 90, 99
BspLI GGNNCC 1 cut(s) 6
BspTI CTTAAG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 44, 78
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 2 cut(s) 90, 99
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstMWI GCNNNNNNNGC 2 cut(s) 54, 96
BstUI CGCG 2 cut(s) 90, 99
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 94
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 77
CseI GACGC 1 cut(s) 88
CviAII CATG 1 cut(s) 37
CviJI RGCY 7 cut(s) 5, 25, 43, 83, 167, 184, 220
CviKI_1 RGCY 7 cut(s) 5, 25, 43, 83, 167, 184, 220
DdeI CTNAG 2 cut(s) 44, 78
Eco147I AGGCCT 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 70
FaeI CATG 1 cut(s) 40
FaiI YATR 1 cut(s) 38
FatI CATG 1 cut(s) 36
FauI CCCGC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 58, 88
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 58, 88
GlaI GCGC 1 cut(s) 76
GluI GCNGC 2 cut(s) 58, 88
GsaI CCCAGC 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 1 cut(s) 83
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 77
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HphI GGTGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 8
HpyAV CCTTC 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 96
HpyF3I CTNAG 2 cut(s) 44, 78
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 75
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 4 cut(s) 7, 21, 97, 153
Lsp1109I GCAGC 1 cut(s) 44
MboII GAAGA 1 cut(s) 94
MnlI CCTC 4 cut(s) 62, 171, 205, 208
MseI TTAA 2 cut(s) 194, 222
MslI CAYNNNNRTG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 221
MvnI CGCG 2 cut(s) 90, 99
MwoI GCNNNNNNNGC 2 cut(s) 54, 96
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 6
OliI CACNNNNGTG 1 cut(s) 110
PceI AGGCCT 1 cut(s) 83
PkrI GCNGC 2 cut(s) 59, 89
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 110
SaqAI TTAA 2 cut(s) 194, 222
SatI GCNGC 2 cut(s) 58, 88
SetI ASST 3 cut(s) 45, 54, 165
SmiMI CAYNNNNRTG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 29, 221
SmoI CTYRAG 2 cut(s) 29, 221
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 3 cut(s) 88, 97, 115
StuI AGGCCT 1 cut(s) 83
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 2 cut(s) 194, 222
Tru9I TTAA 2 cut(s) 194, 222
TseI GCWGC 1 cut(s) 57
TspDTI ATGAA 2 cut(s) 53, 218
Vha464I CTTAAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.