MD10G1109900.v1.1

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012175
Physical Location & Seq
Reverse (-)
18057382 .. 18057864
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1109900.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGCCGCAGAGAAACAGTACTGGCTTCTCAAGACAGAGCCGGGAGAGTGGTCGTGGGAGGACCAAGCGGCCAACGGAGGCGTAACCAAGTGGGACGGCGTCAAGAACAAGCAAGCACAGAAGTACCTCAAGTCCATGAAGCTCAGTGACCTCTGCTTCTTCTACCACTCCGGTGCCAAGGCCCGCCGCGTGGTTGGTGTAGTGAGCGTCGTGCGAGAATGGTATTCGGAAGGCGGGGATGATGGGGTGGTTGATGTGAAGGCGGTTGGGGAGATGAGGAGGCCGGTGGACTTGAAGGAGATGAAAGGGGATCAGGGGCTCAAGGGGTTTGCTCTGTTTCGGCAGCCGAGGCTTTCGGTGGTGCCGGTTTCGGAGGATGTTTGGATGAAGATTTGTGATTTGGGAAGTGGTTATGAAGGTGATGGAAATGCTTTTGAGGATGATGGTAGTGGTGGTGAATCTGGTGGAGAAGGAGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

161

Amino Acids

17.59

Weight (kDa)

4.85

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 133 1.8e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 173, 361
AccII CGCG 1 cut(s) 189
AciI CCGC 6 cut(s) 6, 68, 184, 187, 234, 263
AclWI GGATC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 3, 69
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 20, 125
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 1 cut(s) 295
AleI CACNNNNGTG 1 cut(s) 171
AluBI AGCT 1 cut(s) 142
AluI AGCT 1 cut(s) 142
AlwI GGATC 1 cut(s) 318
AoxI GGCC 4 cut(s) 3, 69, 180, 281
ApeKI GCWGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 61, 181
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 431, 467
AvaII GGWCC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 107, 140
BanI GGYRCC 2 cut(s) 173, 361
BanII GRGCYC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 355
BccI CCATC 3 cut(s) 236, 416, 437
BceAI ACGGC 1 cut(s) 112
BcnI CCSGG 1 cut(s) 42
BisI GCNGC 4 cut(s) 6, 69, 187, 344
BlsI GCNGC 4 cut(s) 7, 70, 188, 345
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 61, 181
BmiI GGNNCC 2 cut(s) 175, 363
BmrFI CCNGG 1 cut(s) 42
BpuEI CTTGAG 3 cut(s) 14, 113, 305
BpuMI CCSGG 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 177, 347
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse118I RCCGGY 2 cut(s) 283, 364
Bse1I ACTGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 177, 347
BseGI GGATG 4 cut(s) 244, 382, 390, 445
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 4 cut(s) 5, 71, 182, 283
BshNI GGYRCC 2 cut(s) 173, 361
BsiSI CCGG 4 cut(s) 41, 171, 284, 365
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 4 cut(s) 5, 71, 182, 283
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 6 cut(s) 6, 68, 184, 187, 234, 263
BspANI GGCC 4 cut(s) 5, 71, 182, 283
BspCNI CTCAG 1 cut(s) 156
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 175, 363
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 2 cut(s) 173, 361
BsrFI RCCGGY 2 cut(s) 283, 364
BsrI ACTGG 1 cut(s) 26
BssAI RCCGGY 2 cut(s) 283, 364
BssECI CCNNGG 2 cut(s) 177, 347
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 18
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 114, 184
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 4 cut(s) 244, 382, 390, 445
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 355
BsuRI GGCC 4 cut(s) 5, 71, 182, 283
BtsCI GGATG 4 cut(s) 244, 382, 390, 445
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 114, 184
Cfr10I RCCGGY 2 cut(s) 283, 364
Cfr13I GGNCC 2 cut(s) 61, 181
CseI GACGC 2 cut(s) 88, 196
Csp6I GTAC 2 cut(s) 19, 124
CviAII CATG 1 cut(s) 136
CviQI GTAC 2 cut(s) 19, 124
DdeI CTNAG 1 cut(s) 143
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 3, 69
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 139
FaiI YATR 2 cut(s) 137, 414
FaqI GGGAC 1 cut(s) 107
FatI CATG 1 cut(s) 135
FauI CCCGC 2 cut(s) 191, 227
Fnu4HI GCNGC 4 cut(s) 6, 69, 187, 344
FokI GGATG 4 cut(s) 251, 389, 397, 452
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 4 cut(s) 6, 69, 187, 344
GluI GCNGC 4 cut(s) 6, 69, 187, 344
HaeIII GGCC 4 cut(s) 5, 71, 182, 283
HapII CCGG 4 cut(s) 41, 171, 284, 365
HgaI GACGC 2 cut(s) 88, 196
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 1 cut(s) 139
HinfI GANTC 1 cut(s) 458
HpaII CCGG 4 cut(s) 41, 171, 284, 365
HphI GGTGA 2 cut(s) 431, 467
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 229, 373
Hpy188III TCNNGA 2 cut(s) 31, 103
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 5 cut(s) 224, 253, 289, 410, 464
HpyCH4III ACNGT 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 1 cut(s) 349
HpyF3I CTNAG 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 1 cut(s) 139
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 7, 54, 184, 297, 299, 378, 447
Lsp1109I GCAGC 1 cut(s) 355
MaeIII GTNAC 2 cut(s) 82, 146
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 2 cut(s) 151, 400
MhlI GDGCHC 1 cut(s) 321
MnlI CCTC 9 cut(s) 52, 71, 137, 161, 270, 273, 342, 367, 430
MslI CAYNNNNRTG 1 cut(s) 171
MspI CCGG 4 cut(s) 41, 171, 284, 365
MspR9I CCNGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 349
NciI CCSGG 1 cut(s) 42
NdeII GATC 1 cut(s) 310
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 2 cut(s) 175, 363
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 146
OliI CACNNNNGTG 1 cut(s) 171
PfeI GAWTC 1 cut(s) 458
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 4 cut(s) 7, 70, 188, 345
PspN4I GGNNCC 2 cut(s) 175, 363
PspPI GGNCC 2 cut(s) 61, 181
PsyI GACNNNGTC 1 cut(s) 98
RsaI GTAC 2 cut(s) 20, 125
RsaNI GTAC 2 cut(s) 19, 124
RseI CAYNNNNRTG 1 cut(s) 171
SatI GCNGC 4 cut(s) 6, 69, 187, 344
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 2 cut(s) 61, 181
ScaI AGTACT 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 321
SetI ASST 4 cut(s) 129, 144, 153, 421
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 3 cut(s) 29, 128, 320
SmoI CTYRAG 3 cut(s) 29, 128, 320
SsiI CCGC 6 cut(s) 6, 68, 184, 187, 234, 263
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 18
TatI WGTACW 1 cut(s) 18
TauI GCSGC 3 cut(s) 8, 71, 189
TfiI GAWTC 1 cut(s) 458
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 4 cut(s) 152, 317, 401, 429
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 1 cut(s) 151
Tth111I GACNNNGTC 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 61
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.