Rh6AG047600

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012175
Physical Location & Seq
Forward (+)
6920047 .. 6920660
614 bp
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UTR
Exon/CDS
Intron
Rh6AG047600.1

Sequence Viewer

Length: 513 bp
ATGGGCAAAGAGAAGCAGTATTGGCTTCTGAAAACAGAGCCAGGAGAGTGGTCGTGGGAGGACCAAGCAGCCAATGAGGGCATCACCAAGTGGGATGGAGTCAAGAACAAGCAGGCCCAGAAGTACCTCAAGTCCATGAACCTCGACGACCTCTGCTTCTTCTACCACTCCGGCGCCAAGGCCCGCCGCATAGTCGGCGTCGTGACGGTCGTGCGTGAATGGTATTCGGAAGGTGAAGGTGGAGATGGTGCGGTTGATGTGAAGGCAGTTGGGGAGATGAGGAGGCCGATTGACTTGAAGGAGATGAAGGGGGAGGAGGGTCTCAAGGGTTTTGCTCTGTTTCGGCAGCCGAGGCTGTCGGTTGTGCCGGTTCCGGAGGATGTGTGGTTGAAAGTTTGTGATTTGGGAGGTGTTTGTGATAATGCCTCGATGAACCAAGCTACTTTGTATTTTGGATTGTGTTTTATGGGAAATAGTAGAATTGGTTCAGATGTCCATTTTGATTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

170

Amino Acids

19.15

Weight (kDa)

5.49

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 134 4.3e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 3 cut(s) 184, 187, 251
AcyI GRCGYC 2 cut(s) 174, 198
AdeI CACNNNGTG 1 cut(s) 90
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 2 cut(s) 298, 391
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 373
AoxI GGCC 3 cut(s) 114, 180, 284
ApeKI GCWGC 2 cut(s) 68, 346
Asp700I GAANNNNTTC 1 cut(s) 484
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 61, 115, 181
AsuHPI GGTGA 2 cut(s) 76, 245
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 2 cut(s) 80, 358
BccI CCATC 2 cut(s) 89, 239
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 3 cut(s) 69, 187, 347
BlsI GCNGC 3 cut(s) 70, 188, 348
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 61, 115, 181
BmiI GGNNCC 2 cut(s) 175, 372
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 113, 308
BsaHI GRCGYC 2 cut(s) 174, 198
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 177, 350
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bse118I RCCGGY 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 177, 350
BseGI GGATG 2 cut(s) 100, 385
BseRI GAGGAG 2 cut(s) 295, 329
BseXI GCAGC 2 cut(s) 80, 358
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 116, 182, 286
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 3 cut(s) 171, 368, 374
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 116, 182, 286
Bso31I GGTCTC 1 cut(s) 326
Bsp13I TCCGGA 1 cut(s) 373
BspACI CCGC 3 cut(s) 184, 187, 251
BspANI GGCC 3 cut(s) 116, 182, 286
BspEI TCCGGA 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 175, 372
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 177, 350
BssNI GRCGYC 2 cut(s) 174, 198
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 208
BstACI GRCGYC 2 cut(s) 174, 198
BstC8I GCNNGC 2 cut(s) 114, 184
BstF5I GGATG 2 cut(s) 100, 385
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 326
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 22, 195, 352
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 80, 358
BstXI CCANNNNNNTGG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 116, 182, 286
BtsCI GGATG 2 cut(s) 100, 385
Cac8I GCNNGC 2 cut(s) 114, 184
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 61, 115, 181
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 136
CviJI RGCY 9 cut(s) 25, 40, 71, 116, 182, 286, 349, 355, 440
CviKI_1 RGCY 9 cut(s) 25, 40, 71, 116, 182, 286, 349, 355, 440
CviQI GTAC 1 cut(s) 124
DinI GGCGCC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 177
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 139
FaiI YATR 3 cut(s) 137, 191, 467
FatI CATG 1 cut(s) 135
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 3 cut(s) 69, 187, 347
FokI GGATG 2 cut(s) 107, 392
Fsp4HI GCNGC 3 cut(s) 69, 187, 347
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 69, 187, 347
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 3 cut(s) 116, 182, 286
HapII CCGG 3 cut(s) 171, 368, 374
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 2 cut(s) 174, 198
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 1 cut(s) 99
HpaII CCGG 3 cut(s) 171, 368, 374
HphI GGTGA 2 cut(s) 76, 245
Hpy188I TCNGA 3 cut(s) 30, 229, 490
Hpy188III TCNNGA 3 cut(s) 103, 202, 374
Hpy99I CGWCG 2 cut(s) 149, 203
HpyAV CCTTC 5 cut(s) 224, 230, 256, 292, 301
HpyCH4III ACNGT 1 cut(s) 208
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 195, 352
Hsp92I GRCGYC 2 cut(s) 174, 198
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 1 cut(s) 174
KasI GGCGCC 1 cut(s) 173
Kpn2I TCCGGA 1 cut(s) 373
LpnPI CCDG 7 cut(s) 27, 54, 98, 131, 184, 381, 387
Lsp1109I GCAGC 2 cut(s) 80, 358
LweI GCATC 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 1 cut(s) 151
MluCI AATT 1 cut(s) 480
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 108
MroI TCCGGA 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 484
MspI CCGG 3 cut(s) 171, 368, 374
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 22, 195, 352
NarI GGCGCC 1 cut(s) 174
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 2 cut(s) 175, 372
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 484
PkrI GCNGC 3 cut(s) 70, 188, 348
PleI GAGTC 1 cut(s) 107
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 175, 372
PspPI GGNCC 3 cut(s) 61, 115, 181
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 3 cut(s) 69, 187, 347
Sau96I GGNCC 3 cut(s) 61, 115, 181
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 42
SetI ASST 7 cut(s) 129, 144, 153, 235, 241, 412, 442
SfaNI GCATC 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 175
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 128, 323
SmoI CTYRAG 2 cut(s) 128, 323
Sse9I AATT 1 cut(s) 480
SsiI CCGC 3 cut(s) 184, 187, 251
SspDI GGCGCC 1 cut(s) 173
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 2 cut(s) 144, 428
TasI AATT 1 cut(s) 480
TauI GCSGC 1 cut(s) 189
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 68, 346
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 3 cut(s) 152, 320, 446
VpaK11BI GGWCC 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.