RLG00000015146

Thymocyte nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012175
Physical Location & Seq
Reverse (-)
62265020 .. 62266765
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015146

Sequence Viewer

Length: 564 bp
ATGGGCAAAGAGAAGCAGTATTGGCTTCTGAAAACAGAGCCAGGAGAGTGGTCGTGGGAGGACCAAGCAGCCAATGAGGGCATCACCAAGTGGGATGGAGTCAAGAACAAGCAGGCCCAGAAGTACCTCAAGTCCATGAACCTCGACGACCTCTGCTTCTTCTACCACTCCGGCGCCAAGGCCCGCCGCATAGTCGGCGTCGTGACGGTCGTGCGTGAATGGTATTCGGAAGGCGAAGGTGGTGACGGTGCGGTTGATGTGAAGGCAGTTGGGGAGATGAGGAGGCCGGTTGACTTGAAGGAGATGAAGGGGGAGGAGGGTCTCAAGGGTTTTGCTCTGTTTCGGCAGCCGAGGCTGTCGGTTGTGCCGGTTTCGGAGGATGTGTGGTTGAAAGTTTGTGATTTGGGAGGTGGGTTTGAAGGTGATGGTGTTGTTGTGGAAGATGATGAATCTGAGAACTATGCAGTAGATAAATTCACAAATCCGAATCAAATTCTGAGAAGACTGCAGGAAGATGCCATCCAATGTCTTTGTGCAATGATTATGGTAGTTTACAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EVE

Protein Analysis

188

Amino Acids

21.13

Weight (kDa)

4.85

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EVE PF01878 6 - 134 4.8e-37 EVE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 3 cut(s) 184, 187, 251
AcsI RAATTY 2 cut(s) 473, 492
AcyI GRCGYC 2 cut(s) 174, 198
AdeI CACNNNGTG 1 cut(s) 90
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 3 cut(s) 298, 391, 419
AjnI CCWGG 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 326
AoxI GGCC 3 cut(s) 114, 180, 284
ApeKI GCWGC 2 cut(s) 68, 346
ApoI RAATTY 2 cut(s) 473, 492
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 3 cut(s) 61, 115, 181
AsuHPI GGTGA 3 cut(s) 76, 254, 434
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 508
BbvI GCAGC 2 cut(s) 80, 358
BccI CCATC 3 cut(s) 89, 419, 527
BciT130I CCWGG 1 cut(s) 42
BcoDI GTCTC 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 506
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 3 cut(s) 69, 187, 347
BlsI GCNGC 3 cut(s) 70, 188, 348
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 61, 115, 181
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 2 cut(s) 90, 505
BpiI GAAGAC 1 cut(s) 508
BpuEI CTTGAG 2 cut(s) 113, 308
BsaHI GRCGYC 2 cut(s) 174, 198
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 177, 350
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bse118I RCCGGY 2 cut(s) 286, 367
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 177, 350
BseGI GGATG 3 cut(s) 100, 385, 519
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 2 cut(s) 444, 488
BseRI GAGGAG 2 cut(s) 295, 329
BseXI GCAGC 2 cut(s) 80, 358
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 116, 182, 286
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 210
BsiSI CCGG 3 cut(s) 171, 287, 368
BsmAI GTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 116, 182, 286
Bso31I GGTCTC 1 cut(s) 326
BspACI CCGC 3 cut(s) 184, 187, 251
BspANI GGCC 3 cut(s) 116, 182, 286
BspCNI CTCAG 2 cut(s) 445, 489
BspLI GGNNCC 1 cut(s) 175
BspMAI CTGCAG 1 cut(s) 510
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 2 cut(s) 286, 367
BssAI RCCGGY 2 cut(s) 286, 367
BssECI CCNNGG 2 cut(s) 177, 350
BssNI GRCGYC 2 cut(s) 174, 198
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 208, 248
BstACI GRCGYC 2 cut(s) 174, 198
BstC8I GCNNGC 2 cut(s) 114, 184
BstDEI CTNAG 2 cut(s) 453, 497
BstF5I GGATG 3 cut(s) 100, 385, 519
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 326
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 22, 195, 352
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 506
BstV1I GCAGC 2 cut(s) 80, 358
BstV2I GAAGAC 1 cut(s) 508
BstXI CCANNNNNNTGG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 116, 182, 286
BtsCI GGATG 3 cut(s) 100, 385, 519
Cac8I GCNNGC 2 cut(s) 114, 184
CfoI GCGC 1 cut(s) 176
Cfr10I RCCGGY 2 cut(s) 286, 367
Cfr13I GGNCC 3 cut(s) 61, 115, 181
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 136
CviJI RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviKI_1 RGCY 8 cut(s) 25, 40, 71, 116, 182, 286, 349, 355
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 2 cut(s) 453, 497
DinI GGCGCC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 177
EgeI GGCGCC 1 cut(s) 175
EheI GGCGCC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 139
FaiI YATR 4 cut(s) 137, 191, 462, 545
FatI CATG 1 cut(s) 135
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 3 cut(s) 69, 187, 347
FokI GGATG 3 cut(s) 107, 392, 506
Fsp4HI GCNGC 3 cut(s) 69, 187, 347
GlaI GCGC 1 cut(s) 175
GluI GCNGC 3 cut(s) 69, 187, 347
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 3 cut(s) 116, 182, 286
HapII CCGG 3 cut(s) 171, 287, 368
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 2 cut(s) 174, 198
Hin1II CATG 1 cut(s) 139
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 3 cut(s) 99, 449, 487
HpaII CCGG 3 cut(s) 171, 287, 368
HphI GGTGA 3 cut(s) 76, 254, 434
Hpy166II GTNNAC 2 cut(s) 292, 553
Hpy188I TCNGA 6 cut(s) 30, 229, 376, 454, 486, 498
Hpy188III TCNNGA 2 cut(s) 103, 202
Hpy8I GTNNAC 2 cut(s) 292, 553
Hpy99I CGWCG 2 cut(s) 149, 203
HpyAV CCTTC 6 cut(s) 224, 230, 256, 292, 301, 413
HpyCH4III ACNGT 2 cut(s) 208, 248
HpyCH4V TGCA 3 cut(s) 464, 508, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 195, 352
HpyF3I CTNAG 2 cut(s) 453, 497
Hsp92I GRCGYC 2 cut(s) 174, 198
Hsp92II CATG 1 cut(s) 139
HspAI GCGC 1 cut(s) 174
KasI GGCGCC 1 cut(s) 173
LpnPI CCDG 8 cut(s) 27, 54, 98, 131, 184, 300, 381, 494
Lsp1109I GCAGC 2 cut(s) 80, 358
LweI GCATC 2 cut(s) 90, 505
MaeIII GTNAC 2 cut(s) 202, 242
MboII GAAGA 4 cut(s) 151, 452, 513, 524
MluCI AATT 2 cut(s) 473, 492
Mly113I GGCGCC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 108
MspI CCGG 3 cut(s) 171, 287, 368
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 22, 195, 352
NarI GGCGCC 1 cut(s) 174
NlaIII CATG 1 cut(s) 139
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 202, 242
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 449, 487
PkrI GCNGC 3 cut(s) 70, 188, 348
PleI GAGTC 1 cut(s) 107
PluTI GGCGCC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 3 cut(s) 61, 115, 181
PstI CTGCAG 1 cut(s) 510
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 3 cut(s) 69, 187, 347
Sau96I GGNCC 3 cut(s) 61, 115, 181
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 42
SetI ASST 6 cut(s) 129, 144, 153, 241, 412, 424
SfaNI GCATC 2 cut(s) 90, 505
SfcI CTRYAG 1 cut(s) 506
SfoI GGCGCC 1 cut(s) 175
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 2 cut(s) 128, 323
SmoI CTYRAG 2 cut(s) 128, 323
Sse9I AATT 2 cut(s) 473, 492
SsiI CCGC 3 cut(s) 184, 187, 251
SspDI GGCGCC 1 cut(s) 173
SspI AATATT 1 cut(s) 559
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 2 cut(s) 208, 248
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 473, 492
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 2 cut(s) 449, 487
TseFI GTSAC 2 cut(s) 202, 242
TseI GCWGC 2 cut(s) 68, 346
Tsp45I GTSAC 2 cut(s) 202, 242
TspDTI ATGAA 3 cut(s) 152, 320, 462
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 473, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.