Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005171

Physical Location & Seq

Genomic Coordinates Reverse (-)

4709226 .. 4712506

Gene Structure 3281 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g05740.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1407 bp

ATGGGTTCGTCGGCGCCGGCAACCGATTCCGACGAGCCCATAACTCCGGCCGGCCGTCTCTTCCTGCAGCCCCAACTGAACCAAATAATCCACTGCGTCATGGGCTTCAAGAACCCAATCGACGTCGAGTCCATCAAATCCCAAGTCAAATCCTCCCTCATGCTCACCCACCCAAGATTCTCCAGCCTCATGGTGCGTGACTCCCACGGCCGAGAACGCTGGCACAAAACCACCCACATCGACCTCGACCGCCACGTCATCGTCCTCCACGACCCCGTCTCCTCCTCCGGTGACCACGAGACCGCCGTCAACGACTACCTGGCCGACCTCTCCACCGGCTCGGGGCTCCCCACGGATAAACCCATGTGGGAATTTCATCTCCTTATTGCTCACAACGTCGGGATTTTCCGAATCCACCATGCTCTGGGAGATGGGATTTCTCTCATGTCTATGTTCTTGGCGAGTTGCCGGAGAGCCGACGACAAGGGGAAGCTGCCGACCTTGGCGTCGGGGAAGAGTAAGGGAGTGAATAAGAATAAGAAAGGGTTGTTGGGTTTGGTGTGTGGGTTTGTGGCAATGGTTTGGTTTAGCTTGGTGTTTGTGGTGGAGTTTGTGCTGAGGTGTTTGTGGGTTTGTGATCGGAAAACTGAGATCAGTGGTGGTGATGGGGTTGAGCTCTGGCCTAGGAAACTAGCCACTGCAAAGTTTAGGATTCAAGATATGAAGCATGTGAAGAAAGCTATTCCTAATGCGACCATCAATGATGTACTTTTTGGGGTGATTTCATTAGGGATATCAAGATACTTGGATCACAGAAAACCAAATGCTTTGCCTGAGGGTGTTCAAATTACAGGGGTAGCCATGACTAATCTAAGAGAGCAACCTGGATTGCAGGAGCTGTCCGATTTGATGAGGAGCAATTCAGGATCAAGTTGGGGTAACAAGTTCGGCATGCTTCTCCTACCTATATATTATCATAACTCTAAGAGCAATGCCGACCCTCTAGCCTATCTCAAGAGATCGAAGGTGATGATCGACAAGAAGAAGCAATCTGTAGAGGGTCATTTCTCATACAAAATCGGTTTGTTTGTAATGACCTACTTGGGACCAAAGGTTGCTACTTGGCTTAATTACCGGATTCTCTGCAATACTAGCTTTACTATTTCAAATATACTCGGACCACAAGAAGAAATTACAGTTGGAGGCAATCCTATAACTTACCTAAGAGCCAGTTCATCAAGTTTGCCCCATGCACTTACAATGCACATGGTGAGCTATGCTGAAAGGGCAGACATGCAAATATTGGTGGCCAAAGATATCATCCCTGACCCCGCATTTCTAGCCAAGTGCTTTGAAGAGGCCTTGCTTGATATGAAGGAAGCAGCTGCAGCCATCACAGAAACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0047196 GO:0071704 GO:0071944 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1298 WES_acyltransf

Protein Analysis

469

Amino Acids

51.84

Weight (kDa)

8.89

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WS_DGAT_cat	PF03007	68 - 256	3.6e-08	Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C	PF06974	312 - 459	4.2e-42	WS/DGAT C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005171)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g05740 FvH4_2g05740
malus_domestica	MD02G1104100.v1.1 MD05G1084200.v1.1 MD05G1084400.v1.1
prunus_persica	Prupe.8G128500_v2.0.a1 Prupe.8G128900_v2.0.a1 Prupe.8G129100_v2.0.a1
pyrus_communis	pycom05g08350 pycom10g07990
rosa_chinensis	RchiOBHm_Chr6g0256641
rosa_laevigata	RLG00000014747 RLG00000023074
rosa_multiflora	Rmu_co8379855.1_g000001 Rmu_sc0007004.1_g000003 Rmu_sc0039060.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395780 Rroxscaffold_7G00209140
rosa_rugosa	Rorug05G0565300 Rorug05G0565300
rosa_samantha	Rh3AG010700 Rh3BG010500 Rh3CG010200 Rh3DG010900 Rh5DG222300 Rh6AG083100 Rh6BG077700 Rh6CG072300 Rh6DG069500
rosa_wichuraiana	Rw6G007500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	254, 505
AatII	GACGTC	1 cut(s)	126
AccB1I	GGYRCC	1 cut(s)	13
AccB7I	CCANNNNNTGG	1 cut(s)	424
AciI	CCGC	3 cut(s)	250, 303, 1332
AclWI	GGATC	2 cut(s)	816, 934
AcoI	YGGCCR	5 cut(s)	48, 52, 208, 321, 1308
AcsI	RAATTY	1 cut(s)	371
AcyI	GRCGYC	3 cut(s)	14, 123, 506
AdeI	CACNNNGTG	1 cut(s)	1270
AfaI	GTAC	1 cut(s)	768
AfiI	CCNNNNNNNGG	2 cut(s)	342, 424
AgsI	TTSAA	5 cut(s)	109, 716, 845, 1167, 1355
AhdI	GACNNNNNGTC	1 cut(s)	127
AjiI	CACGTC	1 cut(s)	256
AjnI	CCWGG	2 cut(s)	318, 883
AjuI	GAANNNNNNNTTGG	4 cut(s)	533, 565, 1182, 1214
AluBI	AGCT	8 cut(s)	493, 591, 676, 740, 898, 1155, 1275, 1385
AluI	AGCT	8 cut(s)	493, 591, 676, 740, 898, 1155, 1275, 1385
Alw21I	GWGCWC	1 cut(s)	678
Alw26I	GTCTC	3 cut(s)	62, 283, 293
AlwI	GGATC	2 cut(s)	816, 934
AlwNI	CAGNNNCTG	1 cut(s)	898
Ama87I	CYCGRG	1 cut(s)	340
AoxI	GGCC	7 cut(s)	48, 52, 208, 321, 680, 1308, 1359
ApeKI	GCWGC	5 cut(s)	67, 493, 1382, 1385, 1388
ApoI	RAATTY	1 cut(s)	371
ArsI	GACNNNNNNTTYG	2 cut(s)	129, 161
AspA2I	CCTAGG	1 cut(s)	683
AspLEI	GCGC	1 cut(s)	16
AspS9I	GGNCC	2 cut(s)	1106, 1178
AsuHPI	GGTGA	6 cut(s)	157, 302, 674, 790, 1039, 1282
AvaI	CYCGRG	1 cut(s)	340
AvaII	GGWCC	2 cut(s)	1106, 1178
AvrII	CCTAGG	1 cut(s)	683
AxyI	CCTNAGG	1 cut(s)	834
BalI	TGGCCA	1 cut(s)	1310
BanI	GGYRCC	1 cut(s)	13
BanII	GRGCYC	3 cut(s)	39, 348, 678
BauI	CACGAG	1 cut(s)	296
Bbv12I	GWGCWC	1 cut(s)	678
BbvCI	CCTCAGC	1 cut(s)	617
BbvI	GCAGC	5 cut(s)	79, 480, 1372, 1394, 1400
BccI	CCATC	5 cut(s)	140, 425, 659, 764, 1400
BceAI	ACGGC	3 cut(s)	39, 223, 290
BciT130I	CCWGG	2 cut(s)	320, 885
BcoDI	GTCTC	3 cut(s)	62, 283, 293
BfaI	CTAG	5 cut(s)	684, 692, 1004, 1152, 1340
BfmI	CTRYAG	3 cut(s)	65, 1053, 1386
BfoI	RGCGCY	1 cut(s)	17
BisI	GCNGC	5 cut(s)	68, 494, 1383, 1386, 1389
BlnI	CCTAGG	1 cut(s)	683
BlsI	GCNGC	5 cut(s)	69, 495, 1384, 1387, 1390
Bme1390I	CCNGG	2 cut(s)	320, 885
Bme18I	GGWCC	2 cut(s)	1106, 1178
BmeRI	GACNNNNNGTC	1 cut(s)	127
BmeT110I	CYCGRG	1 cut(s)	340
BmgBI	CACGTC	1 cut(s)	256
BmgT120I	GGNCC	2 cut(s)	1106, 1178
BmiI	GGNNCC	3 cut(s)	15, 347, 1107
BmrFI	CCNGG	2 cut(s)	320, 885
BoxI	GACNNNNGTC	1 cut(s)	305
BpmI	CTGGAG	1 cut(s)	166
Bpu10I	CCTNAGC	1 cut(s)	617
BpuEI	CTTGAG	1 cut(s)	998
BsaHI	GRCGYC	3 cut(s)	14, 123, 506
BsaI	GGTCTC	1 cut(s)	293
BsaJI	CCNNGG	4 cut(s)	205, 351, 501, 683
BsaWI	WCCGGW	2 cut(s)	287, 1134
BsaXI	ACNNNNNCTCC	2 cut(s)	263, 293
Bsc4I	CCNNNNNNNGG	2 cut(s)	342, 424
Bse118I	RCCGGY	3 cut(s)	16, 50, 335
Bse1I	ACTGG	1 cut(s)	1230
Bse21I	CCTNAGG	1 cut(s)	834
Bse3DI	GCAATG	2 cut(s)	582, 997
BseBI	CCWGG	2 cut(s)	320, 885
BseDI	CCNNGG	4 cut(s)	205, 351, 501, 683
BseGI	GGATG	1 cut(s)	1320
BseLI	CCNNNNNNNGG	2 cut(s)	342, 424
BseMI	GCAATG	2 cut(s)	582, 997
BseMII	CTCAG	3 cut(s)	608, 639, 825
BseNI	ACTGG	1 cut(s)	1230
BseRI	GAGGAG	3 cut(s)	271, 274, 928
BseX3I	CGGCCG	3 cut(s)	48, 52, 208
BseXI	GCAGC	5 cut(s)	79, 480, 1372, 1394, 1400
Bsh1285I	CGRYCG	4 cut(s)	51, 55, 211, 250
BshFI	GGCC	7 cut(s)	50, 54, 210, 323, 682, 1310, 1361
BshNI	GGYRCC	1 cut(s)	13
BsiEI	CGRYCG	4 cut(s)	51, 55, 211, 250
BsiHKAI	GWGCWC	1 cut(s)	678
BsiHKCI	CYCGRG	1 cut(s)	340
BsiSI	CCGG	7 cut(s)	17, 47, 51, 288, 336, 469, 1135
BslFI	GGGAC	1 cut(s)	1119
BslI	CCNNNNNNNGG	2 cut(s)	342, 424
BsmAI	GTCTC	3 cut(s)	62, 283, 293
BsmBI	CGTCTC	2 cut(s)	62, 283
BsmFI	GGGAC	1 cut(s)	1119
BsnI	GGCC	7 cut(s)	50, 54, 210, 323, 682, 1310, 1361
Bso31I	GGTCTC	1 cut(s)	293
BsoBI	CYCGRG	1 cut(s)	340
Bsp1286I	GDGCHC	3 cut(s)	39, 348, 678
Bsp143I	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
BspACI	CCGC	3 cut(s)	250, 303, 1332
BspANI	GGCC	7 cut(s)	50, 54, 210, 323, 682, 1310, 1361
BspCNI	CTCAG	3 cut(s)	609, 640, 826
BspLI	GGNNCC	3 cut(s)	15, 347, 1107
BspMAI	CTGCAG	2 cut(s)	69, 1390
BspPI	GGATC	2 cut(s)	816, 934
BspT107I	GGYRCC	1 cut(s)	13
BspTNI	GGTCTC	1 cut(s)	293
BsrDI	GCAATG	2 cut(s)	582, 997
BsrFI	RCCGGY	3 cut(s)	16, 50, 335
BsrI	ACTGG	1 cut(s)	1230
BssAI	RCCGGY	3 cut(s)	16, 50, 335
BssECI	CCNNGG	4 cut(s)	205, 351, 501, 683
BssMI	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
BssNI	GRCGYC	3 cut(s)	14, 123, 506
BssSI	CACGAG	1 cut(s)	296
BssT1I	CCWWGG	2 cut(s)	501, 683
Bst2BI	CACGAG	1 cut(s)	296
Bst2UI	CCWGG	2 cut(s)	320, 885
Bst4CI	ACNGT	1 cut(s)	1198
Bst6I	CTCTTC	3 cut(s)	65, 509, 1350
BstACI	GRCGYC	3 cut(s)	14, 123, 506
BstC8I	GCNNGC	4 cut(s)	18, 52, 221, 953
BstDEI	CTNAG	6 cut(s)	617, 648, 834, 872, 984, 1223
BstDSI	CCRYGG	2 cut(s)	205, 351
BstEII	GGTNACC	1 cut(s)	290
BstF5I	GGATG	1 cut(s)	1320
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	1 cut(s)	16
BstKTI	GATC	6 cut(s)	640, 654, 811, 929, 1022, 1035
BstMAI	GTCTC	3 cut(s)	62, 283, 293
BstMBI	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
BstMCI	CGRYCG	4 cut(s)	51, 55, 211, 250
BstMWI	GCNNNNNNNGC	6 cut(s)	102, 216, 1152, 1286, 1340, 1388
BstNI	CCWGG	2 cut(s)	320, 885
BstNSI	RCATGY	3 cut(s)	731, 955, 1297
BstPAI	GACNNNNGTC	1 cut(s)	305
BstPI	GGTNACC	1 cut(s)	290
BstSCI	CCNGG	2 cut(s)	318, 883
BstSFI	CTRYAG	3 cut(s)	65, 1053, 1386
BstV1I	GCAGC	5 cut(s)	79, 480, 1372, 1394, 1400
BstXI	CCANNNNNNTGG	1 cut(s)	190
BstZI	CGGCCG	3 cut(s)	48, 52, 208
Bsu36I	CCTNAGG	1 cut(s)	834
BsuRI	GGCC	7 cut(s)	50, 54, 210, 323, 682, 1310, 1361
BtgI	CCRYGG	2 cut(s)	205, 351
BtrI	CACGTC	1 cut(s)	256
BtsCI	GGATG	1 cut(s)	1320
BtsI	GCAGTG	2 cut(s)	91, 696
BtsIMutI	CAGTG	3 cut(s)	91, 661, 696
Cac8I	GCNNGC	4 cut(s)	18, 52, 221, 953
CaiI	CAGNNNCTG	1 cut(s)	898
CfoI	GCGC	1 cut(s)	16
Cfr10I	RCCGGY	3 cut(s)	16, 50, 335
Cfr13I	GGNCC	2 cut(s)	1106, 1178
CseI	GACGC	2 cut(s)	85, 495
Csp6I	GTAC	1 cut(s)	767
CviQI	GTAC	1 cut(s)	767
DdeI	CTNAG	6 cut(s)	617, 648, 834, 872, 984, 1223
DinI	GGCGCC	1 cut(s)	15
DpnI	GATC	6 cut(s)	639, 653, 810, 928, 1021, 1034
DpnII	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
DraIII	CACNNNGTG	1 cut(s)	1270
DrdI	GACNNNNNNGTC	2 cut(s)	254, 505
DriI	GACNNNNNGTC	1 cut(s)	127
DseDI	GACNNNNNNGTC	2 cut(s)	254, 505
EaeI	YGGCCR	5 cut(s)	48, 52, 208, 321, 1308
EagI	CGGCCG	3 cut(s)	48, 52, 208
Eam1104I	CTCTTC	3 cut(s)	65, 509, 1350
Eam1105I	GACNNNNNGTC	1 cut(s)	127
EarI	CTCTTC	3 cut(s)	65, 509, 1350
Ecl136II	GAGCTC	1 cut(s)	676
EclXI	CGGCCG	3 cut(s)	48, 52, 208
Eco130I	CCWWGG	2 cut(s)	501, 683
Eco147I	AGGCCT	1 cut(s)	1361
Eco24I	GRGCYC	3 cut(s)	39, 348, 678
Eco31I	GGTCTC	1 cut(s)	293
Eco32I	GATATC	2 cut(s)	795, 1318
Eco47I	GGWCC	2 cut(s)	1106, 1178
Eco52I	CGGCCG	3 cut(s)	48, 52, 208
Eco53kI	GAGCTC	1 cut(s)	676
Eco81I	CCTNAGG	1 cut(s)	834
Eco88I	CYCGRG	1 cut(s)	340
Eco91I	GGTNACC	1 cut(s)	290
EcoICRI	GAGCTC	1 cut(s)	676
EcoO65I	GGTNACC	1 cut(s)	290
EcoRII	CCWGG	2 cut(s)	318, 883
EcoRV	GATATC	2 cut(s)	795, 1318
EcoT14I	CCWWGG	2 cut(s)	501, 683
EcoT38I	GRGCYC	3 cut(s)	39, 348, 678
EgeI	GGCGCC	1 cut(s)	15
EheI	GGCGCC	1 cut(s)	15
ErhI	CCWWGG	2 cut(s)	501, 683
Esp3I	CGTCTC	2 cut(s)	62, 283
FaqI	GGGAC	1 cut(s)	1119
FauI	CCCGC	1 cut(s)	1339
Fnu4HI	GCNGC	5 cut(s)	68, 494, 1383, 1386, 1389
FokI	GGATG	1 cut(s)	1307
FriOI	GRGCYC	3 cut(s)	39, 348, 678
FseI	GGCCGGCC	1 cut(s)	54
Fsp4HI	GCNGC	5 cut(s)	68, 494, 1383, 1386, 1389
FspBI	CTAG	5 cut(s)	684, 692, 1004, 1152, 1340
GlaI	GCGC	1 cut(s)	15
GluI	GCNGC	5 cut(s)	68, 494, 1383, 1386, 1389
GsuI	CTGGAG	1 cut(s)	166
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	7 cut(s)	50, 54, 210, 323, 682, 1310, 1361
HapII	CCGG	7 cut(s)	17, 47, 51, 288, 336, 469, 1135
HgaI	GACGC	2 cut(s)	85, 495
HhaI	GCGC	1 cut(s)	16
Hin1I	GRCGYC	3 cut(s)	14, 123, 506
Hin6I	GCGC	1 cut(s)	14
HinP1I	GCGC	1 cut(s)	14
HincII	GTYRAC	1 cut(s)	310
HindII	GTYRAC	1 cut(s)	310
HinfI	GANTC	7 cut(s)	26, 128, 177, 200, 411, 712, 1138
HpaII	CCGG	7 cut(s)	17, 47, 51, 288, 336, 469, 1135
HphI	GGTGA	6 cut(s)	157, 302, 674, 790, 1039, 1282
Hpy166II	GTNNAC	1 cut(s)	310
Hpy188I	TCNGA	5 cut(s)	31, 410, 642, 904, 1178
Hpy188III	TCNNGA	6 cut(s)	109, 400, 716, 798, 924, 1015
Hpy8I	GTNNAC	1 cut(s)	310
Hpy99I	CGWCG	7 cut(s)	13, 35, 125, 128, 401, 482, 511
HpyAV	CCTTC	2 cut(s)	1018, 1369
HpyCH4III	ACNGT	1 cut(s)	1198
HpyCH4IV	ACGT	4 cut(s)	123, 255, 396, 1403
HpyCH4V	TGCA	8 cut(s)	67, 701, 892, 1146, 1253, 1264, 1297, 1388
HpyF10VI	GCNNNNNNNGC	6 cut(s)	102, 216, 1152, 1286, 1340, 1388
HpyF3I	CTNAG	6 cut(s)	617, 648, 834, 872, 984, 1223
HpySE526I	ACGT	4 cut(s)	123, 255, 396, 1403
Hsp92I	GRCGYC	3 cut(s)	14, 123, 506
HspAI	GCGC	1 cut(s)	14
KasI	GGCGCC	1 cut(s)	13
KroI	GCCGGC	2 cut(s)	16, 50
KroNI	GCCGGC	2 cut(s)	18, 52
Kzo9I	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
LmnI	GCTCC	3 cut(s)	351, 895, 915
Lsp1109I	GCAGC	5 cut(s)	79, 480, 1372, 1394, 1400
MaeI	CTAG	5 cut(s)	684, 692, 1004, 1152, 1340
MaeII	ACGT	4 cut(s)	123, 255, 396, 1403
MaeIII	GTNAC	3 cut(s)	197, 290, 938
MalI	GATC	6 cut(s)	639, 653, 810, 928, 1021, 1034
MboI	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
MboII	GAAGA	6 cut(s)	52, 526, 745, 1054, 1199, 1367
MhlI	GDGCHC	3 cut(s)	39, 348, 678
MlsI	TGGCCA	1 cut(s)	1310
MluCI	AATT	5 cut(s)	371, 846, 919, 1129, 1191
MluNI	TGGCCA	1 cut(s)	1310
Mly113I	GGCGCC	1 cut(s)	14
MlyI	GAGTC	2 cut(s)	137, 194
MmeI	TCCRAC	2 cut(s)	54, 1180
Mox20I	TGGCCA	1 cut(s)	1310
MroNI	GCCGGC	2 cut(s)	16, 50
MscI	TGGCCA	1 cut(s)	1310
MseI	TTAA	1 cut(s)	1128
MslI	CAYNNNNRTG	1 cut(s)	449
Msp20I	TGGCCA	1 cut(s)	1310
MspA1I	CMGCKG	1 cut(s)	1385
MspI	CCGG	7 cut(s)	17, 47, 51, 288, 336, 469, 1135
MspR9I	CCNGG	2 cut(s)	320, 885
MvaI	CCWGG	2 cut(s)	320, 885
MwoI	GCNNNNNNNGC	6 cut(s)	102, 216, 1152, 1286, 1340, 1388
NaeI	GCCGGC	2 cut(s)	18, 52
NarI	GGCGCC	1 cut(s)	14
NdeII	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
NgoMIV	GCCGGC	2 cut(s)	16, 50
NlaIV	GGNNCC	3 cut(s)	15, 347, 1107
NmeAIII	GCCGAG	1 cut(s)	236
NmuCI	GTSAC	2 cut(s)	197, 290
NspI	RCATGY	3 cut(s)	731, 955, 1297
PaeI	GCATGC	1 cut(s)	955
PceI	AGGCCT	1 cut(s)	1361
PcsI	WCGNNNNNNNCGW	3 cut(s)	252, 267, 303
PdiI	GCCGGC	2 cut(s)	18, 52
PfeI	GAWTC	5 cut(s)	26, 177, 411, 712, 1138
PflFI	GACNNNGTC	1 cut(s)	275
PflMI	CCANNNNNTGG	1 cut(s)	424
PkrI	GCNGC	5 cut(s)	69, 495, 1384, 1387, 1390
PleI	GAGTC	2 cut(s)	136, 194
PluTI	GGCGCC	1 cut(s)	17
PpsI	GAGTC	2 cut(s)	136, 194
PshAI	GACNNNNGTC	1 cut(s)	305
Psp124BI	GAGCTC	1 cut(s)	678
Psp6I	CCWGG	2 cut(s)	318, 883
PspEI	GGTNACC	1 cut(s)	290
PspGI	CCWGG	2 cut(s)	318, 883
PspN4I	GGNNCC	3 cut(s)	15, 347, 1107
PspPI	GGNCC	2 cut(s)	1106, 1178
PstI	CTGCAG	2 cut(s)	69, 1390
PstNI	CAGNNNCTG	1 cut(s)	898
PsyI	GACNNNGTC	1 cut(s)	275
PvuII	CAGCTG	1 cut(s)	1385
RigI	GGCCGGCC	1 cut(s)	54
RsaI	GTAC	1 cut(s)	768
RsaNI	GTAC	1 cut(s)	767
RseI	CAYNNNNRTG	1 cut(s)	449
SacI	GAGCTC	1 cut(s)	678
SaqAI	TTAA	1 cut(s)	1128
SatI	GCNGC	5 cut(s)	68, 494, 1383, 1386, 1389
Sau3AI	GATC	6 cut(s)	637, 651, 808, 926, 1019, 1032
Sau96I	GGNCC	2 cut(s)	1106, 1178
SchI	GAGTC	2 cut(s)	137, 194
ScrFI	CCNGG	2 cut(s)	320, 885
SduI	GDGCHC	3 cut(s)	39, 348, 678
SfcI	CTRYAG	3 cut(s)	65, 1053, 1386
SfoI	GGCGCC	1 cut(s)	15
SinI	GGWCC	2 cut(s)	1106, 1178
SmiMI	CAYNNNNRTG	1 cut(s)	449
SmlI	CTYRAG	1 cut(s)	1013
SmoI	CTYRAG	1 cut(s)	1013
SphI	GCATGC	1 cut(s)	955
Sse9I	AATT	5 cut(s)	371, 846, 919, 1129, 1191
SseBI	AGGCCT	1 cut(s)	1361
SsiI	CCGC	3 cut(s)	250, 303, 1332
SspDI	GGCGCC	1 cut(s)	13
SspI	AATATT	1 cut(s)	1302
SspMI	CTAG	5 cut(s)	684, 692, 1004, 1152, 1340
SstI	GAGCTC	1 cut(s)	678
StuI	AGGCCT	1 cut(s)	1361
StyD4I	CCNGG	2 cut(s)	318, 883
StyI	CCWWGG	2 cut(s)	501, 683
TaaI	ACNGT	1 cut(s)	1198
TaiI	ACGT	4 cut(s)	126, 258, 399, 1406
TaqI	TCGA	6 cut(s)	120, 126, 240, 246, 1022, 1035
TasI	AATT	5 cut(s)	371, 846, 919, 1129, 1191
TatI	WGTACW	1 cut(s)	766
TfiI	GAWTC	5 cut(s)	26, 177, 411, 712, 1138
Tru1I	TTAA	1 cut(s)	1128
Tru9I	TTAA	1 cut(s)	1128
TscAI	CASTG	3 cut(s)	98, 661, 703
TseFI	GTSAC	2 cut(s)	197, 290
TseI	GCWGC	5 cut(s)	67, 493, 1382, 1385, 1388
Tsp45I	GTSAC	2 cut(s)	197, 290
TspDTI	ATGAA	5 cut(s)	365, 737, 774, 1224, 1388
TspGWI	ACGGA	1 cut(s)	368
TspRI	CASTG	3 cut(s)	98, 661, 703
Tth111I	GACNNNGTC	1 cut(s)	275
Van91I	CCANNNNNTGG	1 cut(s)	424
VpaK11BI	GGWCC	2 cut(s)	1106, 1178
XapI	RAATTY	1 cut(s)	371
XceI	RCATGY	3 cut(s)	731, 955, 1297
XmaJI	CCTAGG	1 cut(s)	683
XspI	CTAG	5 cut(s)	684, 692, 1004, 1152, 1340
ZraI	GACGTC	1 cut(s)	124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.