Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005171

Physical Location & Seq

Genomic Coordinates Forward (+)

8343483 .. 8358540

Gene Structure 15058 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1104100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGGATACGTCGGCAGCCGATGACCACTCCGACGAACCCTTGACCCCAGTAGGTCGGCTCCTTCTCCAACCCGAAATGAACCAAATAATCCACTGTGCCATGGGATTCAAAAACTCCATAGACATTGACGCCGTAAAGTCTCACCTCAAAACCTCCCTTCTCCTCTCCCACCCAAGATTCTCAAGCCTCATGCTCCGCGACTCCCGCGGCCTCCAGCACTGGCACAATGCCCCCTACGTTGACCTCGACCGCCACATCATTGTCATCCAGAACCCCGTCACCACATCTCCCGTCGATCACGAGACCGCAGTTAACGAATACTTGGCCGACCTCTCCACAAGCTCTACTAGACTGAGCGCGAACAAACCCCTCTGGGAACTTCACCTCCTCATGGCCCACAACTGCGGCGTCTTCCGAATCCACCACGCATTGGGAGACGGGGTTTCGGTCATGTCCCTATTCTTGGAGAGTTTCAGGACGAGAGGCTCAGATGGAAATGAAGAAAAGATTTTGGGTCGTGGAAGGAGGAAGAGAGTGAATGGTGAAAAGGGATGGTGGGGTTTGTTGATTGGGTATGTGGGGACGTTGTGGTTTAGCTTGGTGTTTTTGGTGGAGCATGCGATGAGGAGCTTGTGGCTTTGTGACCAGAAAACAGCGATAAGTGGCGGCGAGGGAGTTGAGCTCTGGCCTAGGAAGCTGGCCACGGCTAGGTTTCGGCTTCAGGACATGAAGCTCGTCAAGAAAGCTGTTCCGAATGCGACCATCAACGATGTTCTTGTTGGCGTGGTTTCGTCAGGGCTGTCAAGGTACCTAGATCACCAAACACCAAATGCTCTGCATGACGGAATTCGAATTACAGGCCTAGCTATGGTCAATTTAAGAAAGCAACCTGGATTGCAGGTATCCAATTCGTGTTTGGAAAGCAACTCTGAATCAAGCTGGGGTAACAAGTTCGGCATGTTTCTCCTACCTATTTATTACCATAAAAGCAGTGATCTTGATCCTCTAGTGTACCTCAAGAGAGCTAAGCTGGTTCTTGACAGGAAGAAACAATCTCTAGAAGCTGATTTTTCGTACAAAGTTGGATGTTTTCTAATCACCTACTTTGGAGCAAAGGTTGCTAGTTGGCTTCTTTACAAGATGGTCTGCAATACTAGCTTTACCATTTCAAATATACTTGGCCCGCAAGAAGAAATTGCAATTGGGGGCAACCTTGTAACTTACCTTAAAGTGAATACATGCAGCCTACCCCAGGCACTTACAATGCACATGGTGAGCTATGCTGAAAGAGCTGACCTGCAAATCTTGGTGGCCAAAGATATCATTCCGGATCCAACATTTCTCGCAAAATGCTTTGAAGAGGCATTGCTTGATATGAAGGAAGCAGCATCCAGCCATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0010025 GO:0010166 GO:0012505 GO:0016020 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0047196 GO:0071704 GO:0071944 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1298 WES_acyltransf

Protein Analysis

468

Amino Acids

52.2

Weight (kDa)

7.68

Isoelectric Point (pI)

39.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WS_DGAT_cat	PF03007	72 - 258	2.2e-09	Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C	PF06974	314 - 460	4.7e-40	WS/DGAT C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005171)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g05740 FvH4_2g05740
malus_domestica	MD02G1104100.v1.1 MD05G1084200.v1.1 MD05G1084400.v1.1
prunus_persica	Prupe.8G128500_v2.0.a1 Prupe.8G128900_v2.0.a1 Prupe.8G129100_v2.0.a1
pyrus_communis	pycom05g08350 pycom10g07990
rosa_chinensis	RchiOBHm_Chr6g0256641
rosa_laevigata	RLG00000014747 RLG00000023074
rosa_multiflora	Rmu_co8379855.1_g000001 Rmu_sc0007004.1_g000003 Rmu_sc0039060.1_g000001
rosa_roxburghii	Rroxscaffold_6G00395780 Rroxscaffold_7G00209140
rosa_rugosa	Rorug05G0565300 Rorug05G0565300
rosa_samantha	Rh3AG010700 Rh3BG010500 Rh3CG010200 Rh3DG010900 Rh5DG222300 Rh6AG083100 Rh6BG077700 Rh6CG072300 Rh6DG069500
rosa_wichuraiana	Rw6G007500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	889, 1305
Acc65I	GGTACC	1 cut(s)	807
AccB1I	GGYRCC	1 cut(s)	807
AccB7I	CCANNNNNTGG	1 cut(s)	430
AccII	CGCG	3 cut(s)	198, 207, 359
AccIII	TCCGGA	1 cut(s)	1327
AciI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1184
AclWI	GGATC	3 cut(s)	995, 1325, 1338
AcoI	YGGCCR	3 cut(s)	324, 699, 1311
AcsI	RAATTY	1 cut(s)	846
AcuI	CTGAAG	1 cut(s)	704
AcyI	GRCGYC	2 cut(s)	129, 408
AdeI	CACNNNGTG	1 cut(s)	1273
AfaI	GTAC	3 cut(s)	809, 1013, 1076
AfiI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 868, 1252
AgsI	TTSAA	3 cut(s)	109, 1170, 1358
AjnI	CCWGG	2 cut(s)	889, 1251
AjuI	GAANNNNNNNTTGG	2 cut(s)	1185, 1217
AloI	GAACNNNNNNTCC	2 cut(s)	369, 401
Alw21I	GWGCWC	1 cut(s)	684
Alw26I	GTCTC	3 cut(s)	144, 296, 429
AlwI	GGATC	3 cut(s)	995, 1325, 1338
Aor13HI	TCCGGA	1 cut(s)	1327
AoxI	GGCC	8 cut(s)	208, 324, 393, 686, 699, 859, 1180, 1311
ApeKI	GCWGC	3 cut(s)	14, 1242, 1385
ApoI	RAATTY	1 cut(s)	846
Asp718I	GGTACC	1 cut(s)	807
AspA2I	CCTAGG	1 cut(s)	689
AspLEI	GCGC	1 cut(s)	359
AspS9I	GGNCC	2 cut(s)	394, 1181
AsuHPI	GGTGA	7 cut(s)	134, 271, 374, 554, 809, 1090, 1285
AsuII	TTCGAA	1 cut(s)	850
AvrII	CCTAGG	1 cut(s)	689
BalI	TGGCCA	2 cut(s)	701, 1313
BamHI	GGATCC	1 cut(s)	1330
BanI	GGYRCC	1 cut(s)	807
BanII	GRGCYC	1 cut(s)	684
BauI	CACGAG	1 cut(s)	299
BbsI	GAAGAC	1 cut(s)	403
Bbv12I	GWGCWC	1 cut(s)	684
BbvI	GCAGC	3 cut(s)	26, 1254, 1397
BccI	CCATC	4 cut(s)	485, 546, 770, 1135
BceAI	ACGGC	2 cut(s)	116, 720
BciT130I	CCWGG	2 cut(s)	891, 1253
BciVI	GTATCC	1 cut(s)	913
BcoDI	GTCTC	3 cut(s)	144, 296, 429
BfaI	CTAG	9 cut(s)	348, 690, 708, 812, 863, 1007, 1058, 1122, 1155
BfuAI	ACCTGC	2 cut(s)	889, 1305
BfuI	GTATCC	1 cut(s)	913
BisI	GCNGC	6 cut(s)	15, 208, 406, 667, 1243, 1386
BlnI	CCTAGG	1 cut(s)	689
BlpI	GCTNAGC	1 cut(s)	1026
BlsI	GCNGC	6 cut(s)	16, 209, 407, 668, 1244, 1387
Bme1390I	CCNGG	2 cut(s)	891, 1253
BmgT120I	GGNCC	2 cut(s)	394, 1181
BmiI	GGNNCC	3 cut(s)	59, 809, 1332
BmrFI	CCNGG	2 cut(s)	891, 1253
BmrI	ACTGGG	1 cut(s)	41
BmsI	GCATC	1 cut(s)	1397
BmuI	ACTGGG	1 cut(s)	41
BpiI	GAAGAC	1 cut(s)	403
BplI	GAGNNNNNCTC	2 cut(s)	666, 698
BpmI	CTGGAG	1 cut(s)	197
Bpu1102I	GCTNAGC	1 cut(s)	1026
Bpu14I	TTCGAA	1 cut(s)	850
BpuEI	CTTGAG	2 cut(s)	166, 1001
BsaBI	GATNNNNATC	1 cut(s)	999
BsaHI	GRCGYC	2 cut(s)	129, 408
BsaI	GGTCTC	1 cut(s)	296
BsaJI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1251
BsaWI	WCCGGW	1 cut(s)	1327
BsaXI	ACNNNNNCTCC	8 cut(s)	271, 301, 369, 399, 426, 456, 1101, 1131
Bsc4I	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 868, 1252
Bse1I	ACTGG	2 cut(s)	47, 224
Bse3DI	GCAATG	1 cut(s)	1364
Bse8I	GATNNNNATC	1 cut(s)	999
BseAI	TCCGGA	1 cut(s)	1327
BseBI	CCWGG	2 cut(s)	891, 1253
BseDI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1251
BseGI	GGATG	4 cut(s)	264, 557, 1091, 1388
BseJI	GATNNNNATC	1 cut(s)	999
BseLI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 868, 1252
BseMI	GCAATG	1 cut(s)	1364
BseMII	CTCAG	2 cut(s)	344, 501
BseNI	ACTGG	2 cut(s)	47, 224
BseRI	GAGGAG	3 cut(s)	152, 377, 640
BseXI	GCAGC	3 cut(s)	26, 1254, 1397
BseYI	CCCAGC	1 cut(s)	939
Bsh1236I	CGCG	3 cut(s)	198, 207, 359
Bsh1285I	CGRYCG	1 cut(s)	250
BshFI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 861, 1182, 1313
BshNI	GGYRCC	1 cut(s)	807
BsiEI	CGRYCG	1 cut(s)	250
BsiHKAI	GWGCWC	1 cut(s)	684
BsiSI	CCGG	1 cut(s)	1328
BslFI	GGGAC	2 cut(s)	439, 595
BslI	CCNNNNNNNGG	6 cut(s)	391, 430, 463, 708, 868, 1252
BsmAI	GTCTC	3 cut(s)	144, 296, 429
BsmBI	CGTCTC	1 cut(s)	429
BsmFI	GGGAC	2 cut(s)	439, 595
BsmI	GAATGC	1 cut(s)	760
BsnI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 861, 1182, 1313
Bso31I	GGTCTC	1 cut(s)	296
Bsp119I	TTCGAA	1 cut(s)	850
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp13I	TCCGGA	1 cut(s)	1327
Bsp143I	GATC	5 cut(s)	295, 814, 994, 1000, 1330
Bsp1720I	GCTNAGC	1 cut(s)	1026
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1184
BspANI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 861, 1182, 1313
BspCNI	CTCAG	2 cut(s)	345, 500
BspEI	TCCGGA	1 cut(s)	1327
BspFNI	CGCG	3 cut(s)	198, 207, 359
BspLI	GGNNCC	3 cut(s)	59, 809, 1332
BspMI	ACCTGC	2 cut(s)	889, 1305
BspPI	GGATC	3 cut(s)	995, 1325, 1338
BspT104I	TTCGAA	1 cut(s)	850
BspT107I	GGYRCC	1 cut(s)	807
BspTNI	GGTCTC	1 cut(s)	296
BsrDI	GCAATG	1 cut(s)	1364
BsrI	ACTGG	2 cut(s)	47, 224
BssECI	CCNNGG	5 cut(s)	99, 205, 689, 702, 1251
BssMI	GATC	5 cut(s)	295, 814, 994, 1000, 1330
BssNI	GRCGYC	2 cut(s)	129, 408
BssSI	CACGAG	1 cut(s)	299
BssT1I	CCWWGG	2 cut(s)	99, 689
Bst2BI	CACGAG	1 cut(s)	299
Bst2UI	CCWGG	2 cut(s)	891, 1253
Bst4CI	ACNGT	1 cut(s)	95
Bst6I	CTCTTC	2 cut(s)	524, 1353
BstACI	GRCGYC	2 cut(s)	129, 408
BstAPI	GCANNNNNTGC	1 cut(s)	1118
BstBI	TTCGAA	1 cut(s)	850
BstC8I	GCNNGC	3 cut(s)	618, 699, 1184
BstDEI	CTNAG	3 cut(s)	353, 487, 1026
BstDSI	CCRYGG	3 cut(s)	99, 205, 702
BstENI	CCTNNNNNAGG	1 cut(s)	1250
BstF5I	GGATG	4 cut(s)	264, 557, 1091, 1388
BstFNI	CGCG	3 cut(s)	198, 207, 359
BstHHI	GCGC	1 cut(s)	359
BstKTI	GATC	5 cut(s)	298, 817, 997, 1003, 1333
BstMAI	GTCTC	3 cut(s)	144, 296, 429
BstMBI	GATC	5 cut(s)	295, 814, 994, 1000, 1330
BstMCI	CGRYCG	1 cut(s)	250
BstMWI	GCNNNNNNNGC	5 cut(s)	204, 694, 1118, 1155, 1289
BstNI	CCWGG	2 cut(s)	891, 1253
BstNSI	RCATGY	3 cut(s)	620, 961, 1242
BstSCI	CCNGG	2 cut(s)	889, 1251
BstUI	CGCG	3 cut(s)	198, 207, 359
BstV1I	GCAGC	3 cut(s)	26, 1254, 1397
BstV2I	GAAGAC	1 cut(s)	403
BstX2I	RGATCY	1 cut(s)	1330
BstYI	RGATCY	1 cut(s)	1330
BsuI	GTATCC	1 cut(s)	913
BsuRI	GGCC	8 cut(s)	210, 326, 395, 688, 701, 861, 1182, 1313
BtgI	CCRYGG	3 cut(s)	99, 205, 702
BtgZI	GCGATG	1 cut(s)	635
BtsCI	GGATG	4 cut(s)	264, 557, 1091, 1388
BtsI	GCAGTG	1 cut(s)	997
BtsIMutI	CAGTG	3 cut(s)	91, 217, 997
BveI	ACCTGC	2 cut(s)	889, 1305
Cac8I	GCNNGC	3 cut(s)	618, 699, 1184
CfoI	GCGC	1 cut(s)	359
Cfr13I	GGNCC	2 cut(s)	394, 1181
Cfr42I	CCGCGG	1 cut(s)	208
CseI	GACGC	2 cut(s)	137, 397
Csp6I	GTAC	3 cut(s)	808, 1012, 1075
CviQI	GTAC	3 cut(s)	808, 1012, 1075
DdeI	CTNAG	3 cut(s)	353, 487, 1026
DpnI	GATC	5 cut(s)	297, 816, 996, 1002, 1332
DpnII	GATC	5 cut(s)	295, 814, 994, 1000, 1330
DraIII	CACNNNGTG	1 cut(s)	1273
EaeI	YGGCCR	3 cut(s)	324, 699, 1311
Eam1104I	CTCTTC	2 cut(s)	524, 1353
EarI	CTCTTC	2 cut(s)	524, 1353
Ecl136II	GAGCTC	1 cut(s)	682
Eco130I	CCWWGG	2 cut(s)	99, 689
Eco147I	AGGCCT	1 cut(s)	861
Eco24I	GRGCYC	1 cut(s)	684
Eco31I	GGTCTC	1 cut(s)	296
Eco32I	GATATC	1 cut(s)	1321
Eco53kI	GAGCTC	1 cut(s)	682
Eco57I	CTGAAG	1 cut(s)	704
EcoICRI	GAGCTC	1 cut(s)	682
EcoNI	CCTNNNNNAGG	1 cut(s)	1250
EcoRI	GAATTC	1 cut(s)	846
EcoRII	CCWGG	2 cut(s)	889, 1251
EcoRV	GATATC	1 cut(s)	1321
EcoT14I	CCWWGG	2 cut(s)	99, 689
EcoT38I	GRGCYC	1 cut(s)	684
ErhI	CCWWGG	2 cut(s)	99, 689
Esp3I	CGTCTC	1 cut(s)	429
FaqI	GGGAC	2 cut(s)	439, 595
FauI	CCCGC	2 cut(s)	212, 1191
Fnu4HI	GCNGC	6 cut(s)	15, 208, 406, 667, 1243, 1386
FokI	GGATG	4 cut(s)	251, 564, 1098, 1375
FriOI	GRGCYC	1 cut(s)	684
Fsp4HI	GCNGC	6 cut(s)	15, 208, 406, 667, 1243, 1386
FspBI	CTAG	9 cut(s)	348, 690, 708, 812, 863, 1007, 1058, 1122, 1155
GlaI	GCGC	1 cut(s)	358
GluI	GCNGC	6 cut(s)	15, 208, 406, 667, 1243, 1386
GsaI	CCCAGC	1 cut(s)	943
GsuI	CTGGAG	1 cut(s)	197
HaeIII	GGCC	8 cut(s)	210, 326, 395, 688, 701, 861, 1182, 1313
HapII	CCGG	1 cut(s)	1328
HgaI	GACGC	2 cut(s)	137, 397
HhaI	GCGC	1 cut(s)	359
Hin1I	GRCGYC	2 cut(s)	129, 408
Hin6I	GCGC	1 cut(s)	357
HinP1I	GCGC	1 cut(s)	357
HincII	GTYRAC	2 cut(s)	241, 313
HindII	GTYRAC	2 cut(s)	241, 313
HinfI	GANTC	5 cut(s)	105, 177, 200, 417, 932
HpaI	GTTAAC	1 cut(s)	313
HpaII	CCGG	1 cut(s)	1328
HphI	GGTGA	7 cut(s)	134, 271, 374, 554, 809, 1090, 1285
Hpy166II	GTNNAC	3 cut(s)	241, 313, 1012
Hpy188I	TCNGA	5 cut(s)	31, 416, 490, 753, 931
Hpy8I	GTNNAC	3 cut(s)	241, 313, 1012
Hpy99I	CGWCG	3 cut(s)	13, 35, 296
HpyAV	CCTTC	4 cut(s)	71, 167, 516, 1372
HpyCH4III	ACNGT	1 cut(s)	95
HpyCH4IV	ACGT	3 cut(s)	8, 237, 584
HpyCH4V	TGCA	7 cut(s)	838, 898, 1149, 1199, 1242, 1267, 1300
HpyF10VI	GCNNNNNNNGC	5 cut(s)	204, 694, 1118, 1155, 1289
HpyF3I	CTNAG	3 cut(s)	353, 487, 1026
HpySE526I	ACGT	3 cut(s)	8, 237, 584
Hsp92I	GRCGYC	2 cut(s)	129, 408
HspAI	GCGC	1 cut(s)	357
Kpn2I	TCCGGA	1 cut(s)	1327
KpnI	GGTACC	1 cut(s)	811
KspAI	GTTAAC	1 cut(s)	313
KspI	CCGCGG	1 cut(s)	208
Kzo9I	GATC	5 cut(s)	295, 814, 994, 1000, 1330
LmnI	GCTCC	5 cut(s)	63, 198, 613, 627, 1109
Lsp1109I	GCAGC	3 cut(s)	26, 1254, 1397
LweI	GCATC	1 cut(s)	1397
MaeI	CTAG	9 cut(s)	348, 690, 708, 812, 863, 1007, 1058, 1122, 1155
MaeII	ACGT	3 cut(s)	8, 237, 584
MaeIII	GTNAC	4 cut(s)	277, 641, 944, 1216
MalI	GATC	5 cut(s)	297, 816, 996, 1002, 1332
MboI	GATC	5 cut(s)	295, 814, 994, 1000, 1330
MboII	GAAGA	6 cut(s)	403, 512, 541, 1057, 1202, 1370
MfeI	CAATTG	1 cut(s)	1200
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	1 cut(s)	684
MlsI	TGGCCA	2 cut(s)	701, 1313
MluCI	AATT	6 cut(s)	846, 852, 874, 907, 1194, 1200
MluNI	TGGCCA	2 cut(s)	701, 1313
MlyI	GAGTC	1 cut(s)	194
MmeI	TCCRAC	4 cut(s)	54, 91, 1063, 1358
Mox20I	TGGCCA	2 cut(s)	701, 1313
MroI	TCCGGA	1 cut(s)	1327
MscI	TGGCCA	2 cut(s)	701, 1313
MseI	TTAA	3 cut(s)	312, 878, 1227
Msp20I	TGGCCA	2 cut(s)	701, 1313
MspA1I	CMGCKG	1 cut(s)	207
MspI	CCGG	1 cut(s)	1328
MspR9I	CCNGG	2 cut(s)	891, 1253
MunI	CAATTG	1 cut(s)	1200
Mva1269I	GAATGC	1 cut(s)	760
MvaI	CCWGG	2 cut(s)	891, 1253
MvnI	CGCG	3 cut(s)	198, 207, 359
MwoI	GCNNNNNNNGC	5 cut(s)	204, 694, 1118, 1155, 1289
NcoI	CCATGG	1 cut(s)	99
NdeII	GATC	5 cut(s)	295, 814, 994, 1000, 1330
NlaIV	GGNNCC	3 cut(s)	59, 809, 1332
NmuCI	GTSAC	2 cut(s)	277, 641
NspI	RCATGY	3 cut(s)	620, 961, 1242
NspV	TTCGAA	1 cut(s)	850
PaeI	GCATGC	1 cut(s)	620
PceI	AGGCCT	1 cut(s)	861
PcsI	WCGNNNNNNNCGW	1 cut(s)	243
PctI	GAATGC	1 cut(s)	760
PfeI	GAWTC	4 cut(s)	105, 177, 417, 932
PflMI	CCANNNNNTGG	1 cut(s)	430
PkrI	GCNGC	6 cut(s)	16, 209, 407, 668, 1244, 1387
PleI	GAGTC	1 cut(s)	194
PpsI	GAGTC	1 cut(s)	194
Psp124BI	GAGCTC	1 cut(s)	684
Psp6I	CCWGG	2 cut(s)	889, 1251
PspFI	CCCAGC	1 cut(s)	939
PspGI	CCWGG	2 cut(s)	889, 1251
PspN4I	GGNNCC	3 cut(s)	59, 809, 1332
PspPI	GGNCC	2 cut(s)	394, 1181
PsuI	RGATCY	1 cut(s)	1330
RsaI	GTAC	3 cut(s)	809, 1013, 1076
RsaNI	GTAC	3 cut(s)	808, 1012, 1075
SacI	GAGCTC	1 cut(s)	684
SacII	CCGCGG	1 cut(s)	208
SaqAI	TTAA	3 cut(s)	312, 878, 1227
SatI	GCNGC	6 cut(s)	15, 208, 406, 667, 1243, 1386
Sau3AI	GATC	5 cut(s)	295, 814, 994, 1000, 1330
Sau96I	GGNCC	2 cut(s)	394, 1181
SchI	GAGTC	1 cut(s)	194
ScrFI	CCNGG	2 cut(s)	891, 1253
SduI	GDGCHC	1 cut(s)	684
SfaNI	GCATC	1 cut(s)	1397
Sfr303I	CCGCGG	1 cut(s)	208
SfuI	TTCGAA	1 cut(s)	850
SgrBI	CCGCGG	1 cut(s)	208
SmlI	CTYRAG	2 cut(s)	181, 1016
SmoI	CTYRAG	2 cut(s)	181, 1016
SphI	GCATGC	1 cut(s)	620
Sse9I	AATT	6 cut(s)	846, 852, 874, 907, 1194, 1200
SseBI	AGGCCT	1 cut(s)	861
SsiI	CCGC	8 cut(s)	196, 205, 207, 250, 306, 405, 666, 1184
SspMI	CTAG	9 cut(s)	348, 690, 708, 812, 863, 1007, 1058, 1122, 1155
SstI	GAGCTC	1 cut(s)	684
StuI	AGGCCT	1 cut(s)	861
StyD4I	CCNGG	2 cut(s)	889, 1251
StyI	CCWWGG	2 cut(s)	99, 689
TaaI	ACNGT	1 cut(s)	95
TaiI	ACGT	3 cut(s)	11, 240, 587
TaqI	TCGA	3 cut(s)	246, 294, 850
TaqII	GACCGA	1 cut(s)	436
TasI	AATT	6 cut(s)	846, 852, 874, 907, 1194, 1200
TauI	GCSGC	3 cut(s)	210, 408, 669
TfiI	GAWTC	4 cut(s)	105, 177, 417, 932
Tru1I	TTAA	3 cut(s)	312, 878, 1227
Tru9I	TTAA	3 cut(s)	312, 878, 1227
TscAI	CASTG	3 cut(s)	98, 224, 997
TseFI	GTSAC	2 cut(s)	277, 641
TseI	GCWGC	3 cut(s)	14, 1242, 1385
Tsp45I	GTSAC	2 cut(s)	277, 641
TspDTI	ATGAA	4 cut(s)	92, 513, 743, 1391
TspGWI	ACGGA	1 cut(s)	858
TspRI	CASTG	3 cut(s)	98, 224, 997
Van91I	CCANNNNNTGG	1 cut(s)	430
XagI	CCTNNNNNAGG	1 cut(s)	1250
XapI	RAATTY	1 cut(s)	846
XbaI	TCTAGA	1 cut(s)	1057
XceI	RCATGY	3 cut(s)	620, 961, 1242
XcmI	CCANNNNNNNNNTGG	1 cut(s)	913
XmaJI	CCTAGG	1 cut(s)	689
XspI	CTAG	9 cut(s)	348, 690, 708, 812, 863, 1007, 1058, 1122, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.