P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Glyco_hydro_1 | PF00232 | 38 - 510 | 2.4e-158 | Glycosyl hydrolase family 1 |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 251 |
| AccI | GTMKAC | 1 cut(s) | 1150 |
| AccII | CGCG | 1 cut(s) | 1272 |
| AciI | CCGC | 5 cut(s) | 150, 759, 1235, 1270, 1503 |
| AclWI | GGATC | 6 cut(s) | 486, 605, 908, 921, 1079, 1271 |
| AcsI | RAATTY | 1 cut(s) | 572 |
| AcuI | CTGAAG | 1 cut(s) | 1146 |
| AfaI | GTAC | 2 cut(s) | 694, 1391 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 25, 108, 1509 |
| AflII | CTTAAG | 1 cut(s) | 517 |
| AgeI | ACCGGT | 1 cut(s) | 254 |
| AgsI | TTSAA | 3 cut(s) | 545, 855, 1516 |
| AhlI | ACTAGT | 2 cut(s) | 62, 820 |
| AjnI | CCWGG | 1 cut(s) | 658 |
| Alw21I | GWGCWC | 1 cut(s) | 561 |
| AlwI | GGATC | 6 cut(s) | 486, 605, 908, 921, 1079, 1271 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 761 |
| ApeKI | GCWGC | 8 cut(s) | 152, 173, 755, 869, 872, 1055, 1157, 1505 |
| ApoI | RAATTY | 1 cut(s) | 572 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 305, 337 |
| AsiGI | ACCGGT | 1 cut(s) | 254 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 297 |
| AspA2I | CCTAGG | 1 cut(s) | 316 |
| AspS9I | GGNCC | 4 cut(s) | 194, 313, 351, 711 |
| AsuHPI | GGTGA | 5 cut(s) | 269, 590, 592, 941, 1393 |
| AvaII | GGWCC | 4 cut(s) | 194, 313, 351, 711 |
| AvrII | CCTAGG | 1 cut(s) | 316 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 767, 800 |
| BamHI | GGATCC | 1 cut(s) | 913 |
| BanI | GGYRCC | 1 cut(s) | 251 |
| BanII | GRGCYC | 2 cut(s) | 561, 615 |
| Bbv12I | GWGCWC | 1 cut(s) | 561 |
| BbvI | GCAGC | 8 cut(s) | 160, 164, 767, 856, 884, 1067, 1144, 1517 |
| BccI | CCATC | 4 cut(s) | 897, 1254, 1347, 1424 |
| BceAI | ACGGC | 3 cut(s) | 265, 612, 1109 |
| BciT130I | CCWGG | 1 cut(s) | 660 |
| BclI | TGATCA | 1 cut(s) | 271 |
| BcuI | ACTAGT | 2 cut(s) | 62, 820 |
| BfaI | CTAG | 6 cut(s) | 63, 317, 437, 746, 821, 1292 |
| BfmI | CTRYAG | 3 cut(s) | 622, 870, 984 |
| BfrI | CTTAAG | 1 cut(s) | 517 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 633 |
| BglII | AGATCT | 1 cut(s) | 237 |
| BisI | GCNGC | 9 cut(s) | 153, 174, 756, 759, 870, 873, 1056, 1158, 1506 |
| BlnI | CCTAGG | 1 cut(s) | 316 |
| BlsI | GCNGC | 9 cut(s) | 154, 175, 757, 760, 871, 874, 1057, 1159, 1507 |
| Bme1390I | CCNGG | 1 cut(s) | 660 |
| Bme18I | GGWCC | 4 cut(s) | 194, 313, 351, 711 |
| BmgT120I | GGNCC | 4 cut(s) | 194, 313, 351, 711 |
| BmiI | GGNNCC | 3 cut(s) | 253, 712, 915 |
| BmrFI | CCNGG | 1 cut(s) | 660 |
| BmsI | GCATC | 4 cut(s) | 235, 316, 990, 1461 |
| Bsa29I | ATCGAT | 1 cut(s) | 967 |
| BsaBI | GATNNNNATC | 1 cut(s) | 276 |
| BsaJI | CCNNGG | 2 cut(s) | 247, 316 |
| BsaWI | WCCGGW | 3 cut(s) | 108, 254, 644 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 25, 108, 1509 |
| Bse118I | RCCGGY | 1 cut(s) | 254 |
| Bse1I | ACTGG | 3 cut(s) | 160, 700, 836 |
| Bse3DI | GCAATG | 1 cut(s) | 92 |
| Bse8I | GATNNNNATC | 1 cut(s) | 276 |
| BseBI | CCWGG | 1 cut(s) | 660 |
| BseCI | ATCGAT | 1 cut(s) | 967 |
| BseDI | CCNNGG | 2 cut(s) | 247, 316 |
| BseGI | GGATG | 4 cut(s) | 226, 908, 1265, 1270 |
| BseJI | GATNNNNATC | 1 cut(s) | 276 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 25, 108, 1509 |
| BseMI | GCAATG | 1 cut(s) | 92 |
| BseNI | ACTGG | 3 cut(s) | 160, 700, 836 |
| BseRI | GAGGAG | 1 cut(s) | 11 |
| BseXI | GCAGC | 8 cut(s) | 160, 164, 767, 856, 884, 1067, 1144, 1517 |
| Bsh1236I | CGCG | 1 cut(s) | 1272 |
| BshNI | GGYRCC | 1 cut(s) | 251 |
| BshTI | ACCGGT | 1 cut(s) | 254 |
| BshVI | ATCGAT | 1 cut(s) | 967 |
| BsiHKAI | GWGCWC | 1 cut(s) | 561 |
| BsiSI | CCGG | 3 cut(s) | 109, 255, 645 |
| BslFI | GGGAC | 3 cut(s) | 154, 221, 724 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 25, 108, 1509 |
| BsmFI | GGGAC | 3 cut(s) | 154, 221, 724 |
| Bsp1286I | GDGCHC | 2 cut(s) | 561, 615 |
| Bsp143I | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| BspACI | CCGC | 5 cut(s) | 150, 759, 1235, 1270, 1503 |
| BspDI | ATCGAT | 1 cut(s) | 967 |
| BspFNI | CGCG | 1 cut(s) | 1272 |
| BspLI | GGNNCC | 3 cut(s) | 253, 712, 915 |
| BspMAI | CTGCAG | 2 cut(s) | 874, 988 |
| BspPI | GGATC | 6 cut(s) | 486, 605, 908, 921, 1079, 1271 |
| BspT107I | GGYRCC | 1 cut(s) | 251 |
| BspTI | CTTAAG | 1 cut(s) | 517 |
| BsrDI | GCAATG | 1 cut(s) | 92 |
| BsrFI | RCCGGY | 1 cut(s) | 254 |
| BsrI | ACTGG | 3 cut(s) | 160, 700, 836 |
| BssAI | RCCGGY | 1 cut(s) | 254 |
| BssECI | CCNNGG | 2 cut(s) | 247, 316 |
| BssMI | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| BssT1I | CCWWGG | 1 cut(s) | 316 |
| Bst2UI | CCWGG | 1 cut(s) | 660 |
| Bst4CI | ACNGT | 1 cut(s) | 360 |
| BstAFI | CTTAAG | 1 cut(s) | 517 |
| BstC8I | GCNNGC | 2 cut(s) | 947, 1372 |
| BstDEI | CTNAG | 3 cut(s) | 81, 377, 724 |
| BstDSI | CCRYGG | 1 cut(s) | 247 |
| BstF5I | GGATG | 4 cut(s) | 226, 908, 1265, 1270 |
| BstFNI | CGCG | 1 cut(s) | 1272 |
| BstKTI | GATC | 8 cut(s) | 240, 274, 481, 600, 916, 953, 1087, 1279 |
| BstMBI | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 152, 633, 755, 881, 1367 |
| BstNI | CCWGG | 1 cut(s) | 660 |
| BstNSI | RCATGY | 1 cut(s) | 949 |
| BstSCI | CCNGG | 1 cut(s) | 658 |
| BstSFI | CTRYAG | 3 cut(s) | 622, 870, 984 |
| BstUI | CGCG | 1 cut(s) | 1272 |
| BstV1I | GCAGC | 8 cut(s) | 160, 164, 767, 856, 884, 1067, 1144, 1517 |
| BstX2I | RGATCY | 4 cut(s) | 237, 478, 913, 1084 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 204 |
| BstYI | RGATCY | 4 cut(s) | 237, 478, 913, 1084 |
| Bsu15I | ATCGAT | 1 cut(s) | 967 |
| BsuTUI | ATCGAT | 1 cut(s) | 967 |
| BtgI | CCRYGG | 1 cut(s) | 247 |
| BtgZI | GCGATG | 2 cut(s) | 266, 1287 |
| BtsCI | GGATG | 4 cut(s) | 226, 908, 1265, 1270 |
| BtsI | GCAGTG | 1 cut(s) | 981 |
| BtsIMutI | CAGTG | 3 cut(s) | 829, 981, 1248 |
| Cac8I | GCNNGC | 2 cut(s) | 947, 1372 |
| CaiI | CAGNNNCTG | 1 cut(s) | 761 |
| Cfr10I | RCCGGY | 1 cut(s) | 254 |
| Cfr13I | GGNCC | 4 cut(s) | 194, 313, 351, 711 |
| ClaI | ATCGAT | 1 cut(s) | 967 |
| CseI | GACGC | 1 cut(s) | 60 |
| CsiI | ACCWGGT | 1 cut(s) | 658 |
| Csp6I | GTAC | 2 cut(s) | 693, 1390 |
| CspAI | ACCGGT | 1 cut(s) | 254 |
| CviAII | CATG | 5 cut(s) | 348, 752, 919, 946, 1398 |
| CviQI | GTAC | 2 cut(s) | 693, 1390 |
| DdeI | CTNAG | 3 cut(s) | 81, 377, 724 |
| DpnI | GATC | 8 cut(s) | 239, 273, 480, 599, 915, 952, 1086, 1278 |
| DpnII | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| DraI | TTTAAA | 1 cut(s) | 568 |
| Ecl136II | GAGCTC | 1 cut(s) | 559 |
| Eco130I | CCWWGG | 1 cut(s) | 316 |
| Eco24I | GRGCYC | 2 cut(s) | 561, 615 |
| Eco47I | GGWCC | 4 cut(s) | 194, 313, 351, 711 |
| Eco53kI | GAGCTC | 1 cut(s) | 559 |
| Eco57I | CTGAAG | 1 cut(s) | 1146 |
| EcoICRI | GAGCTC | 1 cut(s) | 559 |
| EcoO109I | RGGNCCY | 1 cut(s) | 313 |
| EcoRI | GAATTC | 1 cut(s) | 572 |
| EcoRII | CCWGG | 1 cut(s) | 658 |
| EcoT14I | CCWWGG | 1 cut(s) | 316 |
| EcoT38I | GRGCYC | 2 cut(s) | 561, 615 |
| ErhI | CCWWGG | 1 cut(s) | 316 |
| FaeI | CATG | 5 cut(s) | 351, 755, 922, 949, 1401 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1376, 1408 |
| FaqI | GGGAC | 3 cut(s) | 154, 221, 724 |
| FatI | CATG | 5 cut(s) | 347, 751, 918, 945, 1397 |
| FbaI | TGATCA | 1 cut(s) | 271 |
| FblI | GTMKAC | 1 cut(s) | 1150 |
| Fnu4HI | GCNGC | 9 cut(s) | 153, 174, 756, 759, 870, 873, 1056, 1158, 1506 |
| FokI | GGATG | 4 cut(s) | 213, 915, 1272, 1277 |
| FriOI | GRGCYC | 2 cut(s) | 561, 615 |
| Fsp4HI | GCNGC | 9 cut(s) | 153, 174, 756, 759, 870, 873, 1056, 1158, 1506 |
| FspBI | CTAG | 6 cut(s) | 63, 317, 437, 746, 821, 1292 |
| GluI | GCNGC | 9 cut(s) | 153, 174, 756, 759, 870, 873, 1056, 1158, 1506 |
| HapII | CCGG | 3 cut(s) | 109, 255, 645 |
| HgaI | GACGC | 1 cut(s) | 60 |
| Hin1II | CATG | 5 cut(s) | 351, 755, 922, 949, 1401 |
| HincII | GTYRAC | 3 cut(s) | 391, 535, 1151 |
| HindII | GTYRAC | 3 cut(s) | 391, 535, 1151 |
| HinfI | GANTC | 8 cut(s) | 129, 336, 402, 704, 851, 1103, 1320, 1479 |
| HpaII | CCGG | 3 cut(s) | 109, 255, 645 |
| HphI | GGTGA | 5 cut(s) | 269, 590, 592, 941, 1393 |
| Hpy166II | GTNNAC | 4 cut(s) | 391, 535, 981, 1151 |
| Hpy188I | TCNGA | 3 cut(s) | 303, 850, 1165 |
| Hpy188III | TCNNGA | 4 cut(s) | 230, 354, 545, 1343 |
| Hpy8I | GTNNAC | 4 cut(s) | 391, 535, 981, 1151 |
| HpyAV | CCTTC | 6 cut(s) | 44, 161, 176, 304, 392, 1378 |
| HpyCH4III | ACNGT | 1 cut(s) | 360 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 152, 633, 755, 881, 1367 |
| HpyF3I | CTNAG | 3 cut(s) | 81, 377, 724 |
| Hsp92II | CATG | 5 cut(s) | 351, 755, 922, 949, 1401 |
| Ksp22I | TGATCA | 1 cut(s) | 271 |
| Kzo9I | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| LmnI | GCTCC | 1 cut(s) | 111 |
| Lsp1109I | GCAGC | 8 cut(s) | 160, 164, 767, 856, 884, 1067, 1144, 1517 |
| LweI | GCATC | 4 cut(s) | 235, 316, 990, 1461 |
| MabI | ACCWGGT | 1 cut(s) | 658 |
| MaeI | CTAG | 6 cut(s) | 63, 317, 437, 746, 821, 1292 |
| MaeIII | GTNAC | 9 cut(s) | 55, 89, 360, 460, 578, 729, 825, 929, 1208 |
| MalI | GATC | 8 cut(s) | 239, 273, 480, 599, 915, 952, 1086, 1278 |
| MboI | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| MboII | GAAGA | 4 cut(s) | 247, 305, 473, 509 |
| MfeI | CAATTG | 1 cut(s) | 1143 |
| MflI | RGATCY | 4 cut(s) | 237, 478, 913, 1084 |
| MhlI | GDGCHC | 2 cut(s) | 561, 615 |
| MluCI | AATT | 9 cut(s) | 528, 572, 688, 1033, 1048, 1143, 1283, 1362, 1456 |
| MlyI | GAGTC | 3 cut(s) | 330, 1097, 1473 |
| MmeI | TCCRAC | 1 cut(s) | 99 |
| MnlI | CCTC | 9 cut(s) | 21, 29, 32, 113, 185, 431, 503, 1288, 1313 |
| MroXI | GAANNNNTTC | 1 cut(s) | 297 |
| MseI | TTAA | 7 cut(s) | 518, 567, 588, 605, 1031, 1365, 1377 |
| MslI | CAYNNNNRTG | 2 cut(s) | 346, 1344 |
| MspA1I | CMGCKG | 2 cut(s) | 761, 1157 |
| MspCI | CTTAAG | 1 cut(s) | 517 |
| MspI | CCGG | 3 cut(s) | 109, 255, 645 |
| MspR9I | CCNGG | 1 cut(s) | 660 |
| MunI | CAATTG | 1 cut(s) | 1143 |
| MvaI | CCWGG | 1 cut(s) | 660 |
| MvnI | CGCG | 1 cut(s) | 1272 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 152, 633, 755, 881, 1367 |
| NdeII | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| NlaIII | CATG | 5 cut(s) | 351, 755, 922, 949, 1401 |
| NlaIV | GGNNCC | 3 cut(s) | 253, 712, 915 |
| NmuCI | GTSAC | 7 cut(s) | 55, 89, 460, 578, 729, 825, 929 |
| NspI | RCATGY | 1 cut(s) | 949 |
| PaeI | GCATGC | 1 cut(s) | 949 |
| PdmI | GAANNNNTTC | 1 cut(s) | 297 |
| PfeI | GAWTC | 5 cut(s) | 129, 402, 704, 851, 1320 |
| PinAI | ACCGGT | 1 cut(s) | 254 |
| PkrI | GCNGC | 9 cut(s) | 154, 175, 757, 760, 871, 874, 1057, 1159, 1507 |
| PleI | GAGTC | 3 cut(s) | 330, 1097, 1473 |
| PpsI | GAGTC | 3 cut(s) | 330, 1097, 1473 |
| PpuMI | RGGWCCY | 1 cut(s) | 313 |
| Psp124BI | GAGCTC | 1 cut(s) | 561 |
| Psp5II | RGGWCCY | 1 cut(s) | 313 |
| Psp6I | CCWGG | 1 cut(s) | 658 |
| PspGI | CCWGG | 1 cut(s) | 658 |
| PspN4I | GGNNCC | 3 cut(s) | 253, 712, 915 |
| PspPI | GGNCC | 4 cut(s) | 194, 313, 351, 711 |
| PspPPI | RGGWCCY | 1 cut(s) | 313 |
| PstI | CTGCAG | 2 cut(s) | 874, 988 |
| PstNI | CAGNNNCTG | 1 cut(s) | 761 |
| PsuI | RGATCY | 4 cut(s) | 237, 478, 913, 1084 |
| PvuII | CAGCTG | 1 cut(s) | 1157 |
| RsaI | GTAC | 2 cut(s) | 694, 1391 |
| RsaNI | GTAC | 2 cut(s) | 693, 1390 |
| RseI | CAYNNNNRTG | 2 cut(s) | 346, 1344 |
| SacI | GAGCTC | 1 cut(s) | 561 |
| SalI | GTCGAC | 1 cut(s) | 1149 |
| SaqAI | TTAA | 7 cut(s) | 518, 567, 588, 605, 1031, 1365, 1377 |
| SatI | GCNGC | 9 cut(s) | 153, 174, 756, 759, 870, 873, 1056, 1158, 1506 |
| Sau3AI | GATC | 8 cut(s) | 237, 271, 478, 597, 913, 950, 1084, 1276 |
| Sau96I | GGNCC | 4 cut(s) | 194, 313, 351, 711 |
| SchI | GAGTC | 3 cut(s) | 330, 1097, 1473 |
| ScrFI | CCNGG | 1 cut(s) | 660 |
| SduI | GDGCHC | 2 cut(s) | 561, 615 |
| SexAI | ACCWGGT | 1 cut(s) | 658 |
| SfaNI | GCATC | 4 cut(s) | 235, 316, 990, 1461 |
| SfcI | CTRYAG | 3 cut(s) | 622, 870, 984 |
| SgrAI | CRCCGGYG | 1 cut(s) | 254 |
| SinI | GGWCC | 4 cut(s) | 194, 313, 351, 711 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 346, 1344 |
| SmlI | CTYRAG | 1 cut(s) | 517 |
| SmoI | CTYRAG | 1 cut(s) | 517 |
| SpeI | ACTAGT | 2 cut(s) | 62, 820 |
| SphI | GCATGC | 1 cut(s) | 949 |
| Sse9I | AATT | 9 cut(s) | 528, 572, 688, 1033, 1048, 1143, 1283, 1362, 1456 |
| SsiI | CCGC | 5 cut(s) | 150, 759, 1235, 1270, 1503 |
| SspI | AATATT | 1 cut(s) | 410 |
| SspMI | CTAG | 6 cut(s) | 63, 317, 437, 746, 821, 1292 |
| SstI | GAGCTC | 1 cut(s) | 561 |
| StyD4I | CCNGG | 1 cut(s) | 658 |
| StyI | CCWWGG | 1 cut(s) | 316 |
| TaaI | ACNGT | 1 cut(s) | 360 |
| TaqI | TCGA | 8 cut(s) | 190, 324, 664, 967, 1017, 1097, 1150, 1323 |
| TaqII | GACCGA | 1 cut(s) | 728 |
| TasI | AATT | 9 cut(s) | 528, 572, 688, 1033, 1048, 1143, 1283, 1362, 1456 |
| TatI | WGTACW | 1 cut(s) | 692 |
| TauI | GCSGC | 1 cut(s) | 761 |
| TfiI | GAWTC | 5 cut(s) | 129, 402, 704, 851, 1320 |
| Tru1I | TTAA | 7 cut(s) | 518, 567, 588, 605, 1031, 1365, 1377 |
| Tru9I | TTAA | 7 cut(s) | 518, 567, 588, 605, 1031, 1365, 1377 |
| TscAI | CASTG | 3 cut(s) | 836, 988, 1255 |
| TseFI | GTSAC | 7 cut(s) | 55, 89, 460, 578, 729, 825, 929 |
| TseI | GCWGC | 8 cut(s) | 152, 173, 755, 869, 872, 1055, 1157, 1505 |
| Tsp45I | GTSAC | 7 cut(s) | 55, 89, 460, 578, 729, 825, 929 |
| TspGWI | ACGGA | 1 cut(s) | 522 |
| TspRI | CASTG | 3 cut(s) | 836, 988, 1255 |
| Vha464I | CTTAAG | 1 cut(s) | 517 |
| VpaK11BI | GGWCC | 4 cut(s) | 194, 313, 351, 711 |
| XapI | RAATTY | 1 cut(s) | 572 |
| XceI | RCATGY | 1 cut(s) | 949 |
| XmaJI | CCTAGG | 1 cut(s) | 316 |
| XmiI | GTMKAC | 1 cut(s) | 1150 |
| XmnI | GAANNNNTTC | 1 cut(s) | 297 |
| XspI | CTAG | 6 cut(s) | 63, 317, 437, 746, 821, 1292 |