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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 221 |
| AccB1I | GGYRCC | 1 cut(s) | 263 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 339 |
| AciI | CCGC | 1 cut(s) | 68 |
| AclWI | GGATC | 2 cut(s) | 98, 111 |
| AcsI | RAATTY | 1 cut(s) | 282 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 182, 339, 469 |
| AgsI | TTSAA | 1 cut(s) | 94 |
| AluBI | AGCT | 3 cut(s) | 144, 202, 275 |
| AluI | AGCT | 3 cut(s) | 144, 202, 275 |
| AlwI | GGATC | 2 cut(s) | 98, 111 |
| AoxI | GGCC | 1 cut(s) | 172 |
| ApoI | RAATTY | 1 cut(s) | 282 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 357 |
| AspS9I | GGNCC | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 1 cut(s) | 452 |
| BamHI | GGATCC | 1 cut(s) | 103 |
| BanI | GGYRCC | 1 cut(s) | 263 |
| BccI | CCATC | 1 cut(s) | 146 |
| BmgT120I | GGNCC | 1 cut(s) | 173 |
| BmiI | GGNNCC | 3 cut(s) | 6, 105, 265 |
| BpmI | CTGGAG | 1 cut(s) | 233 |
| BsaJI | CCNNGG | 1 cut(s) | 333 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 182, 339, 469 |
| Bse3DI | GCAATG | 1 cut(s) | 250 |
| BseDI | CCNNGG | 1 cut(s) | 333 |
| BseGI | GGATG | 2 cut(s) | 157, 372 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 182, 339, 469 |
| BseMI | GCAATG | 1 cut(s) | 250 |
| BseMII | CTCAG | 2 cut(s) | 302, 314 |
| BseYI | CCCAGC | 1 cut(s) | 135 |
| BshFI | GGCC | 1 cut(s) | 174 |
| BshNI | GGYRCC | 1 cut(s) | 263 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 182, 339, 469 |
| BsnI | GGCC | 1 cut(s) | 174 |
| Bsp143I | GATC | 3 cut(s) | 21, 103, 205 |
| Bsp19I | CCATGG | 1 cut(s) | 333 |
| BspACI | CCGC | 1 cut(s) | 68 |
| BspANI | GGCC | 1 cut(s) | 174 |
| BspCNI | CTCAG | 2 cut(s) | 301, 313 |
| BspLI | GGNNCC | 3 cut(s) | 6, 105, 265 |
| BspPI | GGATC | 2 cut(s) | 98, 111 |
| BspT107I | GGYRCC | 1 cut(s) | 263 |
| BsrDI | GCAATG | 1 cut(s) | 250 |
| BssECI | CCNNGG | 1 cut(s) | 333 |
| BssMI | GATC | 3 cut(s) | 21, 103, 205 |
| BssT1I | CCWWGG | 1 cut(s) | 333 |
| Bst4CI | ACNGT | 2 cut(s) | 37, 322 |
| BstC8I | GCNNGC | 1 cut(s) | 241 |
| BstDEI | CTNAG | 4 cut(s) | 60, 145, 288, 300 |
| BstDSI | CCRYGG | 1 cut(s) | 333 |
| BstF5I | GGATG | 2 cut(s) | 157, 372 |
| BstKTI | GATC | 3 cut(s) | 24, 106, 208 |
| BstMBI | GATC | 3 cut(s) | 21, 103, 205 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 50, 141, 208, 272 |
| BstNSI | RCATGY | 1 cut(s) | 243 |
| BstX2I | RGATCY | 1 cut(s) | 103 |
| BstYI | RGATCY | 1 cut(s) | 103 |
| BsuRI | GGCC | 1 cut(s) | 174 |
| BtgI | CCRYGG | 1 cut(s) | 333 |
| BtsCI | GGATG | 2 cut(s) | 157, 372 |
| BtsI | GCAGTG | 1 cut(s) | 240 |
| BtsIMutI | CAGTG | 1 cut(s) | 240 |
| Cac8I | GCNNGC | 1 cut(s) | 241 |
| Cfr13I | GGNCC | 1 cut(s) | 173 |
| CseI | GACGC | 1 cut(s) | 430 |
| CviAII | CATG | 5 cut(s) | 100, 240, 334, 339, 468 |
| CviJI | RGCY | 5 cut(s) | 53, 144, 174, 202, 275 |
| CviKI_1 | RGCY | 5 cut(s) | 53, 144, 174, 202, 275 |
| DdeI | CTNAG | 4 cut(s) | 60, 145, 288, 300 |
| DpnI | GATC | 3 cut(s) | 23, 105, 207 |
| DpnII | GATC | 3 cut(s) | 21, 103, 205 |
| Eco130I | CCWWGG | 1 cut(s) | 333 |
| EcoT14I | CCWWGG | 1 cut(s) | 333 |
| ErhI | CCWWGG | 1 cut(s) | 333 |
| FaeI | CATG | 5 cut(s) | 103, 243, 337, 342, 471 |
| FatI | CATG | 5 cut(s) | 99, 239, 333, 338, 467 |
| FauNDI | CATATG | 1 cut(s) | 164 |
| FokI | GGATG | 2 cut(s) | 164, 359 |
| GsaI | CCCAGC | 1 cut(s) | 139 |
| GsuI | CTGGAG | 1 cut(s) | 233 |
| HaeIII | GGCC | 1 cut(s) | 174 |
| HgaI | GACGC | 1 cut(s) | 430 |
| Hin1II | CATG | 5 cut(s) | 103, 243, 337, 342, 471 |
| HindIII | AAGCTT | 2 cut(s) | 142, 200 |
| HinfI | GANTC | 1 cut(s) | 296 |
| HphI | GGTGA | 1 cut(s) | 452 |
| Hpy188I | TCNGA | 2 cut(s) | 190, 438 |
| Hpy99I | CGWCG | 1 cut(s) | 424 |
| HpyAV | CCTTC | 3 cut(s) | 58, 164, 360 |
| HpyCH4III | ACNGT | 2 cut(s) | 37, 322 |
| HpyCH4V | TGCA | 3 cut(s) | 99, 243, 328 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 50, 141, 208, 272 |
| HpyF3I | CTNAG | 4 cut(s) | 60, 145, 288, 300 |
| Hsp92II | CATG | 5 cut(s) | 103, 243, 337, 342, 471 |
| Kzo9I | GATC | 3 cut(s) | 21, 103, 205 |
| LpnPI | CCDG | 2 cut(s) | 121, 197 |
| MalI | GATC | 3 cut(s) | 23, 105, 207 |
| MboI | GATC | 3 cut(s) | 21, 103, 205 |
| MboII | GAAGA | 1 cut(s) | 350 |
| MflI | RGATCY | 1 cut(s) | 103 |
| MluCI | AATT | 1 cut(s) | 282 |
| MnlI | CCTC | 2 cut(s) | 142, 208 |
| MroXI | GAANNNNTTC | 1 cut(s) | 357 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 50, 141, 208, 272 |
| NcoI | CCATGG | 1 cut(s) | 333 |
| NdeI | CATATG | 1 cut(s) | 164 |
| NdeII | GATC | 3 cut(s) | 21, 103, 205 |
| NlaIII | CATG | 5 cut(s) | 103, 243, 337, 342, 471 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 105, 265 |
| NspI | RCATGY | 1 cut(s) | 243 |
| PaeI | GCATGC | 1 cut(s) | 243 |
| PdmI | GAANNNNTTC | 1 cut(s) | 357 |
| PfeI | GAWTC | 1 cut(s) | 296 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 339 |
| PsiI | TTATAA | 1 cut(s) | 221 |
| PspFI | CCCAGC | 1 cut(s) | 135 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 105, 265 |
| PspPI | GGNCC | 1 cut(s) | 173 |
| PsuI | RGATCY | 1 cut(s) | 103 |
| Sau3AI | GATC | 3 cut(s) | 21, 103, 205 |
| Sau96I | GGNCC | 1 cut(s) | 173 |
| SetI | ASST | 4 cut(s) | 146, 204, 219, 277 |
| SphI | GCATGC | 1 cut(s) | 243 |
| Sse9I | AATT | 1 cut(s) | 282 |
| SsiI | CCGC | 1 cut(s) | 68 |
| StyI | CCWWGG | 1 cut(s) | 333 |
| TaaI | ACNGT | 2 cut(s) | 37, 322 |
| TasI | AATT | 1 cut(s) | 282 |
| TfiI | GAWTC | 1 cut(s) | 296 |
| TscAI | CASTG | 1 cut(s) | 240 |
| TspDTI | ATGAA | 1 cut(s) | 151 |
| TspGWI | ACGGA | 1 cut(s) | 378 |
| TspRI | CASTG | 1 cut(s) | 240 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 339 |
| XapI | RAATTY | 1 cut(s) | 282 |
| XceI | RCATGY | 1 cut(s) | 243 |
| XmnI | GAANNNNTTC | 1 cut(s) | 357 |