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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 88 |
| AcuI | CTGAAG | 2 cut(s) | 282, 324 |
| AflIII | ACRYGT | 2 cut(s) | 22, 64 |
| AgsI | TTSAA | 1 cut(s) | 209 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 178, 210 |
| AoxI | GGCC | 1 cut(s) | 124 |
| ApoI | RAATTY | 1 cut(s) | 88 |
| AspS9I | GGNCC | 1 cut(s) | 124 |
| AsuC2I | CCSGG | 1 cut(s) | 337 |
| BcnI | CCSGG | 1 cut(s) | 337 |
| Bme1390I | CCNGG | 1 cut(s) | 337 |
| BmgT120I | GGNCC | 1 cut(s) | 124 |
| BmrFI | CCNGG | 1 cut(s) | 337 |
| BpuMI | CCSGG | 1 cut(s) | 337 |
| Bsa29I | ATCGAT | 1 cut(s) | 320 |
| BsaAI | YACGTR | 1 cut(s) | 65 |
| BseCI | ATCGAT | 1 cut(s) | 320 |
| BshFI | GGCC | 1 cut(s) | 126 |
| BshVI | ATCGAT | 1 cut(s) | 320 |
| BsiSI | CCGG | 1 cut(s) | 336 |
| BsnI | GGCC | 1 cut(s) | 126 |
| BspANI | GGCC | 1 cut(s) | 126 |
| BspDI | ATCGAT | 1 cut(s) | 320 |
| BstBAI | YACGTR | 1 cut(s) | 65 |
| BstNSI | RCATGY | 1 cut(s) | 26 |
| BstSCI | CCNGG | 1 cut(s) | 335 |
| Bsu15I | ATCGAT | 1 cut(s) | 320 |
| BsuRI | GGCC | 1 cut(s) | 126 |
| BsuTUI | ATCGAT | 1 cut(s) | 320 |
| Cfr13I | GGNCC | 1 cut(s) | 124 |
| ClaI | ATCGAT | 1 cut(s) | 320 |
| CviAII | CATG | 1 cut(s) | 23 |
| CviJI | RGCY | 1 cut(s) | 126 |
| CviKI_1 | RGCY | 1 cut(s) | 126 |
| DraI | TTTAAA | 1 cut(s) | 106 |
| Eco57I | CTGAAG | 2 cut(s) | 282, 324 |
| EcoRI | GAATTC | 1 cut(s) | 88 |
| FaeI | CATG | 1 cut(s) | 26 |
| FaiI | YATR | 3 cut(s) | 24, 96, 288 |
| FatI | CATG | 1 cut(s) | 22 |
| HaeIII | GGCC | 1 cut(s) | 126 |
| HapII | CCGG | 1 cut(s) | 336 |
| Hin1II | CATG | 1 cut(s) | 26 |
| HinfI | GANTC | 4 cut(s) | 29, 152, 275, 317 |
| HpaII | CCGG | 1 cut(s) | 336 |
| Hpy188I | TCNGA | 3 cut(s) | 34, 157, 185 |
| HpyAV | CCTTC | 1 cut(s) | 287 |
| HpyCH4IV | ACGT | 1 cut(s) | 64 |
| HpySE526I | ACGT | 1 cut(s) | 64 |
| Hsp92II | CATG | 1 cut(s) | 26 |
| MaeII | ACGT | 1 cut(s) | 64 |
| MfeI | CAATTG | 1 cut(s) | 193 |
| MluCI | AATT | 3 cut(s) | 59, 88, 193 |
| MnlI | CCTC | 4 cut(s) | 110, 142, 229, 283 |
| MseI | TTAA | 1 cut(s) | 105 |
| MspI | CCGG | 1 cut(s) | 336 |
| MspR9I | CCNGG | 1 cut(s) | 337 |
| MunI | CAATTG | 1 cut(s) | 193 |
| NciI | CCSGG | 1 cut(s) | 337 |
| NlaIII | CATG | 1 cut(s) | 26 |
| NspI | RCATGY | 1 cut(s) | 26 |
| PciI | ACATGT | 1 cut(s) | 22 |
| PfeI | GAWTC | 4 cut(s) | 29, 152, 275, 317 |
| Ppu21I | YACGTR | 1 cut(s) | 65 |
| PscI | ACATGT | 1 cut(s) | 22 |
| PspPI | GGNCC | 1 cut(s) | 124 |
| SaqAI | TTAA | 1 cut(s) | 105 |
| Sau96I | GGNCC | 1 cut(s) | 124 |
| ScrFI | CCNGG | 1 cut(s) | 337 |
| SetI | ASST | 1 cut(s) | 67 |
| SgeI | CNNG | 6 cut(s) | 17, 35, 66, 77, 128, 291 |
| Sse9I | AATT | 3 cut(s) | 59, 88, 193 |
| StyD4I | CCNGG | 1 cut(s) | 335 |
| TaiI | ACGT | 1 cut(s) | 67 |
| TaqI | TCGA | 3 cut(s) | 115, 170, 320 |
| TasI | AATT | 3 cut(s) | 59, 88, 193 |
| TfiI | GAWTC | 4 cut(s) | 29, 152, 275, 317 |
| Tru1I | TTAA | 1 cut(s) | 105 |
| Tru9I | TTAA | 1 cut(s) | 105 |
| TspDTI | ATGAA | 3 cut(s) | 282, 324, 338 |
| XapI | RAATTY | 1 cut(s) | 88 |
| XceI | RCATGY | 1 cut(s) | 26 |