FvH4_2g07390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007455
Physical Location & Seq
Forward (+)
6082407 .. 6084383
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07390.t1

Sequence Viewer

Length: 600 bp
ATGGCCTCGGTAGCGCAACAAGGGACTGCTTGCTGTCCCTTCGGATCCTTCCTCGCTGCAGATCGGATCGTTCTTTTTACCTCTTTTCCTTTTTATTTTATTTTGGTTACTATCTTTGAGGATGTTTCGTATGGATTTGCGATTTGTGTTGTGGAGTTTGTTGGTTGGGTTGGGTACTGCTGTGTTTCAACGAGACTGGTTTGGTTGGCTTCAAAGCCGTCGCCAGCGGATCGGGTGACGGTCCTATGTCACCCTCGGAAGACGTCTCTGTGTCAACCTATTGCTGATCGATCTGCTCCTTGGCTGAAGTTTGTAGGATATGTAGGATATGTTTTGATGGAGGGATGGGTGAGCGAGCTGGGGAAGCTGAAGGAGAAGGTTGGGGCCAAGAAGCGGTTTCTGTGGTCGTCGAAAAAGGCGAAGCATAGCGAAGGAGGAGATGAGGGTAAGGTTGATCAGCAGCAACAACCAGCTGTAGCTGCTGCAGCTCATGAAGGAGCTGGGGTGATGGAGAGTGCTGTTAGAAAAGAGAGGAGGAACTCTTCTACGCTTTCTGAGGCCACTGTTTGTTTGCTCATGGACCGTTTTGTTCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

21.93

Weight (kDa)

8.78

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 266
AciI CCGC 2 cut(s) 227, 394
AclWI GGATC 4 cut(s) 39, 52, 74, 237
AcuI CTGAAG 2 cut(s) 326, 389
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 393
AgsI TTSAA 2 cut(s) 189, 213
AluBI AGCT 6 cut(s) 358, 367, 473, 479, 488, 500
AluI AGCT 6 cut(s) 358, 367, 473, 479, 488, 500
Alw26I GTCTC 2 cut(s) 187, 270
AlwI GGATC 4 cut(s) 39, 52, 74, 237
AoxI GGCC 3 cut(s) 3, 384, 558
ApeKI GCWGC 5 cut(s) 56, 460, 479, 482, 485
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 3 cut(s) 241, 384, 580
AsuHPI GGTGA 4 cut(s) 242, 247, 361, 517
AvaII GGWCC 2 cut(s) 241, 580
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
BamHI GGATCC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 266
BbvI GCAGC 5 cut(s) 43, 466, 469, 472, 497
BccI CCATC 3 cut(s) 331, 339, 502
BceAI ACGGC 1 cut(s) 202
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 187, 270
BfmI CTRYAG 3 cut(s) 57, 474, 483
BisI GCNGC 5 cut(s) 57, 461, 480, 483, 486
BlsI GCNGC 5 cut(s) 58, 462, 481, 484, 487
Bme18I GGWCC 2 cut(s) 241, 580
BmgT120I GGNCC 3 cut(s) 241, 384, 580
BmiI GGNNCC 2 cut(s) 46, 385
BpiI GAAGAC 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 6, 254, 299, 593
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 289
BseDI CCNNGG 4 cut(s) 6, 254, 299, 593
BseGI GGATG 2 cut(s) 127, 350
BseLI CCNNNNNNNGG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 450, 547
BseXI GCAGC 5 cut(s) 43, 466, 469, 472, 497
BseYI CCCAGC 2 cut(s) 358, 500
BshFI GGCC 3 cut(s) 5, 386, 560
BshVI ATCGAT 1 cut(s) 289
BslFI GGGAC 2 cut(s) 21, 37
BslI CCNNNNNNNGG 1 cut(s) 393
BsmAI GTCTC 2 cut(s) 187, 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 2 cut(s) 21, 37
BsnI GGCC 3 cut(s) 5, 386, 560
Bsp143I GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
BspACI CCGC 2 cut(s) 227, 394
BspANI GGCC 3 cut(s) 5, 386, 560
BspCNI CTCAG 1 cut(s) 547
BspDI ATCGAT 1 cut(s) 289
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 46, 385
BspMAI CTGCAG 2 cut(s) 61, 487
BspPI GGATC 4 cut(s) 39, 52, 74, 237
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 4 cut(s) 6, 254, 299, 593
BssMI GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 2 cut(s) 299, 593
Bst4CI ACNGT 3 cut(s) 241, 565, 584
Bst6I CTCTTC 1 cut(s) 547
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 3 cut(s) 31, 225, 356
BstDEI CTNAG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 127, 350
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 7 cut(s) 47, 64, 69, 232, 289, 293, 457
BstMAI GTCTC 2 cut(s) 187, 270
BstMBI GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
BstMWI GCNNNNNNNGC 4 cut(s) 11, 364, 479, 485
BstSFI CTRYAG 3 cut(s) 57, 474, 483
BstV1I GCAGC 5 cut(s) 43, 466, 469, 472, 497
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
Bsu15I ATCGAT 1 cut(s) 289
BsuRI GGCC 3 cut(s) 5, 386, 560
BsuTUI ATCGAT 1 cut(s) 289
BtsCI GGATG 2 cut(s) 127, 350
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 3 cut(s) 31, 225, 356
CciI TCATGA 1 cut(s) 490
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 241, 384, 580
ClaI ATCGAT 1 cut(s) 289
Csp6I GTAC 1 cut(s) 175
CviAII CATG 2 cut(s) 491, 577
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 555
DpnI GATC 7 cut(s) 46, 63, 68, 231, 288, 292, 456
DpnII GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
Eam1104I CTCTTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 299, 593
Eco47I GGWCC 2 cut(s) 241, 580
Eco57I CTGAAG 2 cut(s) 326, 389
EcoT14I CCWWGG 2 cut(s) 299, 593
ErhI CCWWGG 2 cut(s) 299, 593
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 2 cut(s) 494, 580
FaiI YATR 7 cut(s) 132, 247, 321, 330, 426, 492, 578
FaqI GGGAC 2 cut(s) 21, 37
FatI CATG 2 cut(s) 490, 576
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 5 cut(s) 57, 461, 480, 483, 486
FokI GGATG 2 cut(s) 134, 357
Fsp4HI GCNGC 5 cut(s) 57, 461, 480, 483, 486
GlaI GCGC 1 cut(s) 15
GluI GCNGC 5 cut(s) 57, 461, 480, 483, 486
GsaI CCCAGC 2 cut(s) 362, 504
HaeIII GGCC 3 cut(s) 5, 386, 560
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 2 cut(s) 494, 580
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 275
HindII GTYRAC 1 cut(s) 275
HphI GGTGA 4 cut(s) 242, 247, 361, 517
Hpy166II GTNNAC 1 cut(s) 275
Hpy188I TCNGA 4 cut(s) 44, 66, 258, 556
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 1 cut(s) 275
Hpy99I CGWCG 2 cut(s) 223, 412
HpyAV CCTTC 6 cut(s) 49, 58, 364, 370, 425, 488
HpyCH4III ACNGT 3 cut(s) 241, 565, 584
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 59, 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 364, 479, 485
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 1 cut(s) 263
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 2 cut(s) 494, 580
HspAI GCGC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
LmnI GCTCC 2 cut(s) 301, 497
LpnPI CCDG 5 cut(s) 182, 237, 344, 483, 486
Lsp1109I GCAGC 5 cut(s) 43, 466, 469, 472, 497
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 3 cut(s) 106, 235, 248
MalI GATC 7 cut(s) 46, 63, 68, 231, 288, 292, 456
MboI GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
MboII GAAGA 2 cut(s) 271, 534
MflI RGATCY 1 cut(s) 44
MspA1I CMGCKG 2 cut(s) 227, 473
MwoI GCNNNNNNNGC 4 cut(s) 11, 364, 479, 485
NdeII GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
NlaIII CATG 2 cut(s) 494, 580
NlaIV GGNNCC 2 cut(s) 46, 385
NmuCI GTSAC 2 cut(s) 235, 248
PagI TCATGA 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PkrI GCNGC 5 cut(s) 58, 462, 481, 484, 487
PspFI CCCAGC 2 cut(s) 358, 500
PspN4I GGNNCC 2 cut(s) 46, 385
PspPI GGNCC 3 cut(s) 241, 384, 580
PstI CTGCAG 2 cut(s) 61, 487
PsuI RGATCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SatI GCNGC 5 cut(s) 57, 461, 480, 483, 486
Sau3AI GATC 7 cut(s) 44, 61, 66, 229, 286, 290, 454
Sau96I GGNCC 3 cut(s) 241, 384, 580
SfcI CTRYAG 3 cut(s) 57, 474, 483
SinI GGWCC 2 cut(s) 241, 580
SsiI CCGC 2 cut(s) 227, 394
StyI CCWWGG 2 cut(s) 299, 593
TaaI ACNGT 3 cut(s) 241, 565, 584
TaiI ACGT 1 cut(s) 266
TaqI TCGA 2 cut(s) 289, 410
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 2 cut(s) 235, 248
TseI GCWGC 5 cut(s) 56, 460, 479, 482, 485
Tsp45I GTSAC 2 cut(s) 235, 248
TspDTI ATGAA 1 cut(s) 507
TspRI CASTG 1 cut(s) 568
VpaK11BI GGWCC 2 cut(s) 241, 580
ZraI GACGTC 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.