Rorug02G0325000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007455
Physical Location & Seq
Reverse (-)
39876802 .. 39878387
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0325000.1

Sequence Viewer

Length: 354 bp
ATGCTCTTCGCCCAAATCGACGCCGTCTACGCCGCCCTCGATCCAACGGTTCCGATTTCGGAAGAGAAAGCAATAGTGATCGAGAAGAGTTATCTGATTCCTGAACTGGAGATTCCCACATCGGTGAGAGTAAGAGTGAAAACTACGAGCTTAAACTACGCTAATTACCTTCAGATACTTGGCAAGTACCAAGAGAAGCCGCCTCTGCCTTTCATCTCCGGCTCCAATTACTCTGGCGTCGTCAACACCGTCAGCCCCGGCGTCTCTAAGTTTAAAGTCGGCGATGCTGTTTGCTCCGTCGCCGCCCTCGGGTCCTTTGCTCAGTTCATCGTCGTCGATCAGTCACTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

12.64

Weight (kDa)

5.28

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 39 - 115 7.6e-12 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 27
AciI CCGC 3 cut(s) 33, 200, 303
AclWI GGATC 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 3 cut(s) 21, 237, 261
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 309
AleI CACNNNNGTG 2 cut(s) 122, 349
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 312
BceAI ACGGC 1 cut(s) 8
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 268
BisI GCNGC 3 cut(s) 33, 200, 303
BlsI GCNGC 3 cut(s) 34, 201, 304
Bme1390I CCNGG 1 cut(s) 258
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 3 cut(s) 51, 223, 313
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 258
BsaHI GRCGYC 3 cut(s) 21, 237, 261
BsaJI CCNNGG 2 cut(s) 256, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 256, 307
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 2 cut(s) 219, 258
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 268
BsmBI CGTCTC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 308
Bsp143I GATC 3 cut(s) 40, 78, 337
BspACI CCGC 3 cut(s) 33, 200, 303
BspCNI CTCAG 1 cut(s) 334
BspLI GGNNCC 3 cut(s) 51, 223, 313
BspPI GGATC 1 cut(s) 35
BspQI GCTCTTC 1 cut(s) 11
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 256, 307
BssMI GATC 3 cut(s) 40, 78, 337
BssNI GRCGYC 3 cut(s) 21, 237, 261
Bst4CI ACNGT 2 cut(s) 49, 250
Bst6I CTCTTC 3 cut(s) 11, 57, 80
BstACI GRCGYC 3 cut(s) 21, 237, 261
BstDEI CTNAG 2 cut(s) 267, 321
BstKTI GATC 3 cut(s) 43, 81, 340
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 3 cut(s) 40, 78, 337
BstMWI GCNNNNNNNGC 2 cut(s) 29, 205
BstSCI CCNGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 3 cut(s) 29, 226, 250
Csp6I GTAC 1 cut(s) 187
CviJI RGCY 4 cut(s) 150, 199, 222, 255
CviKI_1 RGCY 4 cut(s) 150, 199, 222, 255
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 267, 321
DpnI GATC 3 cut(s) 42, 80, 339
DpnII GATC 3 cut(s) 40, 78, 337
DraI TTTAAA 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 11, 57, 80
EarI CTCTTC 3 cut(s) 11, 57, 80
Eco47I GGWCC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 268
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 3 cut(s) 33, 200, 303
Fsp4HI GCNGC 3 cut(s) 33, 200, 303
GluI GCNGC 3 cut(s) 33, 200, 303
GsuI CTGGAG 1 cut(s) 128
HapII CCGG 2 cut(s) 219, 258
HgaI GACGC 3 cut(s) 29, 226, 250
Hin1I GRCGYC 3 cut(s) 21, 237, 261
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 2 cut(s) 97, 112
HpaII CCGG 2 cut(s) 219, 258
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 2 cut(s) 28, 244
Hpy188I TCNGA 4 cut(s) 54, 61, 96, 174
Hpy188III TCNNGA 2 cut(s) 82, 101
Hpy8I GTNNAC 2 cut(s) 28, 244
Hpy99I CGWCG 5 cut(s) 23, 242, 302, 335, 338
HpyAV CCTTC 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 49, 250
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 205
HpyF3I CTNAG 2 cut(s) 267, 321
Hsp92I GRCGYC 3 cut(s) 21, 237, 261
Kzo9I GATC 3 cut(s) 40, 78, 337
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 2 cut(s) 227, 299
LpnPI CCDG 5 cut(s) 92, 114, 219, 232, 271
LweI GCATC 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 342
MalI GATC 3 cut(s) 42, 80, 339
MboI GATC 3 cut(s) 40, 78, 337
MboII GAAGA 2 cut(s) 74, 97
MluCI AATT 2 cut(s) 163, 226
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 3 cut(s) 47, 213, 317
MseI TTAA 2 cut(s) 152, 273
MslI CAYNNNNRTG 2 cut(s) 122, 349
MspI CCGG 2 cut(s) 219, 258
MspR9I CCNGG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 29, 205
NciI CCSGG 1 cut(s) 258
NdeII GATC 3 cut(s) 40, 78, 337
NlaIV GGNNCC 3 cut(s) 51, 223, 313
NmuCI GTSAC 1 cut(s) 342
OliI CACNNNNGTG 2 cut(s) 122, 349
PciSI GCTCTTC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 36, 246
PfeI GAWTC 2 cut(s) 97, 112
PflFI GACNNNGTC 1 cut(s) 23
PkrI GCNGC 3 cut(s) 34, 201, 304
PpuMI RGGWCCY 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 312
PspN4I GGNNCC 3 cut(s) 51, 223, 313
PspPI GGNCC 1 cut(s) 312
PspPPI RGGWCCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 23
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 2 cut(s) 122, 349
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 152, 273
SatI GCNGC 3 cut(s) 33, 200, 303
Sau3AI GATC 3 cut(s) 40, 78, 337
Sau96I GGNCC 1 cut(s) 312
ScrFI CCNGG 1 cut(s) 258
SetI ASST 2 cut(s) 152, 171
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 122, 349
Sse9I AATT 2 cut(s) 163, 226
SsiI CCGC 3 cut(s) 33, 200, 303
StyD4I CCNGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 49, 250
TaqI TCGA 4 cut(s) 18, 39, 81, 336
TasI AATT 2 cut(s) 163, 226
TauI GCSGC 3 cut(s) 35, 202, 305
TfiI GAWTC 2 cut(s) 97, 112
Tru1I TTAA 2 cut(s) 152, 273
Tru9I TTAA 2 cut(s) 152, 273
TseFI GTSAC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 2 cut(s) 202, 316
TspGWI ACGGA 1 cut(s) 286
Tth111I GACNNNGTC 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 312
XmiI GTMKAC 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.