FvH4_2g16390

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001524
Physical Location & Seq
Forward (+)
14325341 .. 14333510
8170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16390.t11

Sequence Viewer

Length: 1464 bp
ATGGTGTCAGCTGCTCTGAGGTTGAGGAAAAGTGAGTTGAGTGACATTCCTGTCCCAGAAGCATATTCTCCAAATGTGAAACGTGAGCTGCTGATGCTTTCCTTACCTGCAATTGTGGGTCAGGCAATTGATCCCATAGCAATGCTGATGGAAACTGCTTATATTGGTAGATTGGGCTCTGTGGAGTTGGCTTCAGCTGGTGTTTCAATGAACATCTTTAATTATATTTCAAAGCTTTTTAATATTCCTCTCCTCAGTGTTGCTACATCTTTTGTGGCTGAAGACATTGCAAAGTCTGCAAGCAAAGCTTCCAGTATAGAAAATGGTTACGAAGGAAATACTAATGGAAAATCTGTACCTACCGAGAGAAAGCAATTATCTTCAGTGTCAACTGCCTTGCTTTTAGCACTTGGCATTGGCTTGTTTGAGGCTGTAGCCTTGTCTTTGGGATCTGGATTGTTTCTGAATATGATGGGGATATCATCGAACTCTCCTATGCACATTCCGGCAAAGAGGTTTCTCATAATAAGAGCGTTTGGTGCTCCTGCAGTTGTAGCATCTTTGGCTCTTCAAGGAGTTTTCCGTGGTTTTAAGGATACAAAAACTCCAGTATTATGTCTAGGTATCGGTAATCTTTTTGCCGCGTTCTTGTTACCAACCCTTATATATTACTTTCGTTTGGGTGTAACTGGTGCAGCCATGTCTACTGTTATATCTCAATACACTGTTACATTTTTAATGATCTGGTTTTTAAATAAGAAAGCAGTTCTACTGCCTCCAAAGATGGGATCATTGCAATTTGGTGGATACATAAAATCTGGTGGTTTTCTTTTGGGAAGAACTCTTGCTGTTCTCACGACCATGACATTGGGAACATCGATGGCTGCTCGTCAAGGTCCAGTGGCCATGGCTGCTCATCAGATATGTATACAAGTATGGTTGGCTGTATCCCTTCTAACTGATGCGCTCGCTACATCTGGTCAGGCCCTGATTGCAAGTTATTCATCCAAAGGTGAATACAAAGTTGTTAAAGAGGTTACTGACTTCATGCTGAAGATAGGATTGTTCATAGGTGTTTCATTGGCTGTAATTTTGGGAACATCTTTTGGTTCTATATCAACCTTGTTCACCAAAGATGCTGCAGTATTAGAGATTCTTAGATCTGGGGTATTGTTTGTCAGTGCTAGTCAACCTTTGACTTCTCTAGCTTATGTTTTCGATGGTCTCCATTATGGTGTTTCAGATTTTGCATATGCAGCTCGTGCCATGATGGTCGTGGGTGCAATATCATCCGGATTTCTGCTATATGCTCCTTCTGTTATGGGTCTTTCTGGGGTTTGGTTGGGTTTGACTCTCTTCATGGGCTTGCGTGCTGCGGCTGGATTTGTCAGATTACTTTCAAAATCAGGTCCATGGTGGTTCCTGCACAGGGACACCGAGACATCTCAGCTTGTAACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

488

Amino Acids

51.66

Weight (kDa)

9.58

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 265 - 354 3.5e-08 MatE
MatE PF01554 425 - 562 8.7e-08 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
Acc36I ACCTGC 1 cut(s) 115
AccI GTMKAC 2 cut(s) 704, 928
AccII CGCG 1 cut(s) 644
AccIII TCCGGA 1 cut(s) 1292
AciI CCGC 2 cut(s) 642, 1376
AclWI GGATC 3 cut(s) 125, 457, 796
AcoI YGGCCR 1 cut(s) 903
AcuI CTGAAG 4 cut(s) 177, 300, 366, 1073
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 785, 1429
AgsI TTSAA 4 cut(s) 207, 231, 572, 1401
AluBI AGCT 8 cut(s) 11, 88, 197, 235, 308, 1208, 1259, 1450
AluI AGCT 8 cut(s) 11, 88, 197, 235, 308, 1208, 1259, 1450
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 1229, 1433
AlwI GGATC 3 cut(s) 125, 457, 796
AlwNI CAGNNNCTG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 1292
AoxI GGCC 2 cut(s) 903, 984
ApeKI GCWGC 8 cut(s) 11, 88, 695, 884, 911, 1139, 1256, 1373
AspLEI GCGC 1 cut(s) 967
AspS9I GGNCC 3 cut(s) 896, 985, 1409
AsuHPI GGTGA 2 cut(s) 1025, 1120
AvaII GGWCC 2 cut(s) 896, 1409
BalI TGGCCA 1 cut(s) 905
BanII GRGCYC 1 cut(s) 179
BauI CACGAG 1 cut(s) 1260
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 7 cut(s) 75, 707, 871, 898, 1126, 1268, 1360
BccI CCATC 6 cut(s) 142, 466, 778, 874, 1214, 1264
BciVI GTATCC 3 cut(s) 589, 800, 958
BcoDI GTCTC 2 cut(s) 1229, 1433
BfaI CTAG 3 cut(s) 620, 1185, 1205
BfmI CTRYAG 3 cut(s) 432, 546, 1140
BfuAI ACCTGC 1 cut(s) 115
BfuI GTATCC 3 cut(s) 589, 800, 958
BglII AGATCT 1 cut(s) 1160
Bme18I GGWCC 2 cut(s) 896, 1409
BmgT120I GGNCC 3 cut(s) 896, 985, 1409
BmiI GGNNCC 1 cut(s) 1421
BmsI GCATC 4 cut(s) 84, 566, 952, 1126
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 878
BsaI GGTCTC 1 cut(s) 1229
BsaJI CCNNGG 3 cut(s) 583, 906, 1412
BsaWI WCCGGW 1 cut(s) 1292
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 2 cut(s) 785, 1429
Bse1I ACTGG 5 cut(s) 312, 608, 694, 899, 1458
Bse3DI GCAATG 3 cut(s) 147, 285, 791
BseAI TCCGGA 1 cut(s) 1292
BseCI ATCGAT 1 cut(s) 878
BseDI CCNNGG 3 cut(s) 583, 906, 1412
BseGI GGATG 2 cut(s) 1004, 1289
BseLI CCNNNNNNNGG 2 cut(s) 785, 1429
BseMI GCAATG 3 cut(s) 147, 285, 791
BseMII CTCAG 3 cut(s) 8, 268, 1460
BseNI ACTGG 5 cut(s) 312, 608, 694, 899, 1458
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 7 cut(s) 75, 707, 871, 898, 1126, 1268, 1360
BsgI GTGCAG 2 cut(s) 714, 1409
Bsh1236I CGCG 1 cut(s) 644
BshFI GGCC 2 cut(s) 905, 986
BshVI ATCGAT 1 cut(s) 878
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 2 cut(s) 506, 1293
BslFI GGGAC 2 cut(s) 38, 1445
BslI CCNNNNNNNGG 2 cut(s) 785, 1429
BsmAI GTCTC 2 cut(s) 1229, 1433
BsmFI GGGAC 2 cut(s) 38, 1445
BsnI GGCC 2 cut(s) 905, 986
Bso31I GGTCTC 1 cut(s) 1229
Bsp1286I GDGCHC 2 cut(s) 179, 544
Bsp13I TCCGGA 1 cut(s) 1292
Bsp143I GATC 5 cut(s) 130, 449, 741, 788, 1160
Bsp19I CCATGG 2 cut(s) 906, 1412
BspACI CCGC 2 cut(s) 642, 1376
BspANI GGCC 2 cut(s) 905, 986
BspCNI CTCAG 3 cut(s) 9, 267, 1459
BspDI ATCGAT 1 cut(s) 878
BspEI TCCGGA 1 cut(s) 1292
BspFNI CGCG 1 cut(s) 644
BspLI GGNNCC 1 cut(s) 1421
BspMAI CTGCAG 2 cut(s) 550, 1144
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 3 cut(s) 125, 457, 796
BspQI GCTCTTC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 1229
BsrDI GCAATG 3 cut(s) 147, 285, 791
BsrI ACTGG 5 cut(s) 312, 608, 694, 899, 1458
BssECI CCNNGG 3 cut(s) 583, 906, 1412
BssMI GATC 5 cut(s) 130, 449, 741, 788, 1160
BssNAI GTATAC 1 cut(s) 929
BssSI CACGAG 1 cut(s) 1260
BssT1I CCWWGG 2 cut(s) 906, 1412
Bst1107I GTATAC 1 cut(s) 929
Bst2BI CACGAG 1 cut(s) 1260
Bst4CI ACNGT 2 cut(s) 709, 727
Bst6I CTCTTC 2 cut(s) 573, 1361
BstAPI GCANNNNNTGC 2 cut(s) 296, 1262
BstC8I GCNNGC 4 cut(s) 301, 969, 1367, 1371
BstDEI CTNAG 4 cut(s) 17, 254, 1157, 1446
BstDSI CCRYGG 3 cut(s) 583, 906, 1412
BstF5I GGATG 2 cut(s) 1004, 1289
BstFNI CGCG 1 cut(s) 644
BstHHI GCGC 1 cut(s) 967
BstKTI GATC 5 cut(s) 133, 452, 744, 791, 1163
BstMAI GTCTC 2 cut(s) 1229, 1433
BstMBI GATC 5 cut(s) 130, 449, 741, 788, 1160
BstSFI CTRYAG 3 cut(s) 432, 546, 1140
BstUI CGCG 1 cut(s) 644
BstV1I GCAGC 7 cut(s) 75, 707, 871, 898, 1126, 1268, 1360
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 2 cut(s) 449, 1160
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 2 cut(s) 449, 1160
BstZ17I GTATAC 1 cut(s) 929
Bsu15I ATCGAT 1 cut(s) 878
BsuI GTATCC 3 cut(s) 589, 800, 958
BsuRI GGCC 2 cut(s) 905, 986
BsuTUI ATCGAT 1 cut(s) 878
BtgI CCRYGG 3 cut(s) 583, 906, 1412
BtsCI GGATG 2 cut(s) 1004, 1289
BtsIMutI CAGTG 5 cut(s) 262, 390, 723, 906, 1186
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 4 cut(s) 301, 969, 1367, 1371
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 1 cut(s) 967
Cfr13I GGNCC 3 cut(s) 896, 985, 1409
ClaI ATCGAT 1 cut(s) 878
Csp6I GTAC 1 cut(s) 356
CviAII CATG 7 cut(s) 700, 862, 907, 1048, 1267, 1360, 1413
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 4 cut(s) 17, 254, 1157, 1446
DpnI GATC 5 cut(s) 132, 451, 743, 790, 1162
DpnII GATC 5 cut(s) 130, 449, 741, 788, 1160
DraI TTTAAA 1 cut(s) 753
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 1 cut(s) 903
Eam1104I CTCTTC 2 cut(s) 573, 1361
EarI CTCTTC 2 cut(s) 573, 1361
Eco130I CCWWGG 2 cut(s) 906, 1412
Eco24I GRGCYC 1 cut(s) 179
Eco31I GGTCTC 1 cut(s) 1229
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 2 cut(s) 896, 1409
Eco57I CTGAAG 4 cut(s) 177, 300, 366, 1073
EcoO109I RGGNCCY 1 cut(s) 985
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 2 cut(s) 906, 1412
EcoT38I GRGCYC 1 cut(s) 179
ErhI CCWWGG 2 cut(s) 906, 1412
FaeI CATG 7 cut(s) 703, 865, 910, 1051, 1270, 1363, 1416
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FaqI GGGAC 2 cut(s) 38, 1445
FatI CATG 7 cut(s) 699, 861, 906, 1047, 1266, 1359, 1412
FauNDI CATATG 1 cut(s) 1252
FblI GTMKAC 2 cut(s) 704, 928
FokI GGATG 2 cut(s) 991, 1276
FriOI GRGCYC 1 cut(s) 179
FspBI CTAG 3 cut(s) 620, 1185, 1205
GlaI GCGC 1 cut(s) 966
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 2 cut(s) 905, 986
HapII CCGG 2 cut(s) 506, 1293
HhaI GCGC 1 cut(s) 967
Hin1II CATG 7 cut(s) 703, 865, 910, 1051, 1270, 1363, 1416
Hin6I GCGC 1 cut(s) 965
HinP1I GCGC 1 cut(s) 965
HincII GTYRAC 2 cut(s) 390, 1190
HindII GTYRAC 2 cut(s) 390, 1190
HindIII AAGCTT 2 cut(s) 233, 306
HinfI GANTC 2 cut(s) 1153, 1351
HpaII CCGG 2 cut(s) 506, 1293
HphI GGTGA 2 cut(s) 1025, 1120
Hpy166II GTNNAC 5 cut(s) 390, 705, 929, 1128, 1190
Hpy188I TCNGA 5 cut(s) 18, 465, 921, 1243, 1391
Hpy188III TCNNGA 3 cut(s) 453, 856, 1293
Hpy8I GTNNAC 5 cut(s) 390, 705, 929, 1128, 1190
HpyAV CCTTC 3 cut(s) 326, 962, 1323
HpyCH4III ACNGT 2 cut(s) 709, 727
HpyCH4IV ACGT 1 cut(s) 82
HpyF3I CTNAG 4 cut(s) 17, 254, 1157, 1446
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 7 cut(s) 703, 865, 910, 1051, 1270, 1363, 1416
HspAI GCGC 1 cut(s) 965
Kpn2I TCCGGA 1 cut(s) 1292
Kzo9I GATC 5 cut(s) 130, 449, 741, 788, 1160
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 2 cut(s) 547, 1315
Lsp1109I GCAGC 7 cut(s) 75, 707, 871, 898, 1126, 1268, 1360
LweI GCATC 4 cut(s) 84, 566, 952, 1126
MaeI CTAG 3 cut(s) 620, 1185, 1205
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 7 cut(s) 41, 326, 651, 685, 727, 1036, 1453
MalI GATC 5 cut(s) 132, 451, 743, 790, 1162
MboI GATC 5 cut(s) 130, 449, 741, 788, 1160
MboII GAAGA 6 cut(s) 293, 372, 560, 849, 1066, 1348
MfeI CAATTG 2 cut(s) 111, 126
MflI RGATCY 2 cut(s) 449, 1160
MhlI GDGCHC 2 cut(s) 179, 544
MlsI TGGCCA 1 cut(s) 905
MluCI AATT 6 cut(s) 111, 126, 220, 374, 797, 1089
MluNI TGGCCA 1 cut(s) 905
MlyI GAGTC 1 cut(s) 1345
MnlI CCTC 8 cut(s) 12, 18, 258, 263, 421, 507, 786, 1027
Mox20I TGGCCA 1 cut(s) 905
MroI TCCGGA 1 cut(s) 1292
MscI TGGCCA 1 cut(s) 905
MseI TTAA 6 cut(s) 219, 240, 591, 737, 752, 1029
MslI CAYNNNNRTG 3 cut(s) 140, 860, 1233
Msp20I TGGCCA 1 cut(s) 905
MspA1I CMGCKG 2 cut(s) 11, 197
MspI CCGG 2 cut(s) 506, 1293
MunI CAATTG 2 cut(s) 111, 126
MvnI CGCG 1 cut(s) 644
NcoI CCATGG 2 cut(s) 906, 1412
NdeI CATATG 1 cut(s) 1252
NdeII GATC 5 cut(s) 130, 449, 741, 788, 1160
NlaIII CATG 7 cut(s) 703, 865, 910, 1051, 1270, 1363, 1416
NlaIV GGNNCC 1 cut(s) 1421
NmuCI GTSAC 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 573
PfeI GAWTC 1 cut(s) 1153
PleI GAGTC 1 cut(s) 1345
PpsI GAGTC 1 cut(s) 1345
PspN4I GGNNCC 1 cut(s) 1421
PspPI GGNCC 3 cut(s) 896, 985, 1409
PstI CTGCAG 2 cut(s) 550, 1144
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 449, 1160
PvuII CAGCTG 2 cut(s) 11, 197
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 3 cut(s) 140, 860, 1233
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 6 cut(s) 219, 240, 591, 737, 752, 1029
Sau3AI GATC 5 cut(s) 130, 449, 741, 788, 1160
Sau96I GGNCC 3 cut(s) 896, 985, 1409
SchI GAGTC 1 cut(s) 1345
SduI GDGCHC 2 cut(s) 179, 544
SfaNI GCATC 4 cut(s) 84, 566, 952, 1126
SfcI CTRYAG 3 cut(s) 432, 546, 1140
SinI GGWCC 2 cut(s) 896, 1409
SmiMI CAYNNNNRTG 3 cut(s) 140, 860, 1233
Sse9I AATT 6 cut(s) 111, 126, 220, 374, 797, 1089
SsiI CCGC 2 cut(s) 642, 1376
SspI AATATT 1 cut(s) 244
SspMI CTAG 3 cut(s) 620, 1185, 1205
StyI CCWWGG 2 cut(s) 906, 1412
TaaI ACNGT 2 cut(s) 709, 727
TaiI ACGT 1 cut(s) 85
TaqI TCGA 3 cut(s) 485, 878, 1218
TasI AATT 6 cut(s) 111, 126, 220, 374, 797, 1089
TauI GCSGC 2 cut(s) 644, 1379
TfiI GAWTC 1 cut(s) 1153
Tru1I TTAA 6 cut(s) 219, 240, 591, 737, 752, 1029
Tru9I TTAA 6 cut(s) 219, 240, 591, 737, 752, 1029
TscAI CASTG 5 cut(s) 262, 390, 730, 906, 1186
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 8 cut(s) 11, 88, 695, 884, 911, 1139, 1256, 1373
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 6 cut(s) 224, 993, 1036, 1057, 1068, 1348
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 5 cut(s) 262, 390, 730, 906, 1186
VpaK11BI GGWCC 2 cut(s) 896, 1409
XcmI CCANNNNNNNNNTGG 1 cut(s) 1273
XmiI GTMKAC 2 cut(s) 704, 928
XspI CTAG 3 cut(s) 620, 1185, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.