Rh6BG244500

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001524
Physical Location & Seq
Forward (+)
45487879 .. 45494485
6607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG244500.1

Sequence Viewer

Length: 1449 bp
ATGAATCACAGTGAGTTGAGTGACATTCCTGTCCCTGAAGCATATTCTCCAAATGTGAAACGCGAGCTGCTGATGCTTTCCATACCTGCAATTGTGGGTCAGGCAATTGATCCCATAGCAATGCTGATGGAGACTGCTTATATTGGTAGACTGGGCTCTGTGGAGTTGGCCTCAGCTGGTGTATCAATGAACATTTTTAATTATATATCAAAACTATTTAATATCCCTCTCCTCAGTGTTGCTACATCTTTTGTGGCTGAAGACATTGCAAAGTCTGCAAGTAAAGCTTCTACTATAGAAAATGCTTATGAAGAAATTACTAATGGAAAATCTGTACCTGCTGAGAGAAAGCAATTATCTTCAGTGTCAACAGCCTTACTTTTAGCACTTGGCATCGGCATGTTTGAGGCGCTAGCCTTGTCTTTGGGATCTGGGTTGTTTCTGAATATGATGGGGATATCATCAAACTCTCCTATGCACATTCCGGCAAAAAGGTTTCTCATAATAAGAGCATTTGGTGCTCCTGCAGTTGTAGCATCTTTGGCTCTTCAAGGAGTTTTCCGTGGTTTTAAGGATACAACAACTCCAGTACTATGTCTAGGTATTGGTAATCTTTTAGCTGCGTTCTTGTTACCAACACTAATATATTATTTTCGTTTGGGTGTAACTGGTGCAGCAATGTCTACTGTTATATCTCAATACACCGTCACATTTTTAATGATCTGGTTTTTAAATAAGAAAGCAGTACTATTGCCTCCAAAGATGGGATCATTGCAATTTGGTGGATACATAAAATCTGGTGGTTTTCTTCTGGGAAGAACTCTTGCTGTTCTCACGACCATGACATTAGGAACATCAATGGCTGCTCGTCAAGGTCCAGTAGCTATGGCTGCTCATCAGATATGTATACAAGTATGGTTGGCTGTATCCCTTCTAACTGATGCACTTGCTACATCTGGTCAGGCCCTGATTGCGAGTTATTCATCAAAAGGCGAATACAAAATTGTTAAAGAGATTACTGACTTCATGCTGAAGATAGGATTGTTTATAGGTGTTTCATTGGCTGTAATTCTGGGAACATCTTTTGGTTCTATAGCGACCTTGTTCACCAAGGATGCTGCAGTATTGGAGATCCTTAGATCTGGGGTATTGTTTGTCAGTGCTAGTCAACCTTTGACTTCTTTAGCTTATGTTTTCGATGGTCTCCATTATGGTGTTTCAGATTTTGCATATGCAGCTCGTGCCATGATGGTCGTGGGTGCAATATCATCTGTATTTCTGCTGTATGCTCCTTCTGTTATCGGTCTTTCTGGGGTTTGGTTGGGTTTGACTCTCTTCATGGGCTTGCGTGCTGTGGCTGGATTTGTCAGATTGCTTTCAAAATCAGGTCCATGGTGGTTCCTGCATAGGGACACTGAGACATCTCAGCTTGTAACCATCGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

482

Amino Acids

51.27

Weight (kDa)

9.22

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 121 - 210 3.5e-07 MatE
MatE PF01554 281 - 419 1.9e-07 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
Acc36I ACCTGC 2 cut(s) 94, 346
AccI GTMKAC 3 cut(s) 148, 683, 907
AccII CGCG 1 cut(s) 63
AclWI GGATC 4 cut(s) 104, 436, 775, 1126
AcuI CTGAAG 4 cut(s) 57, 279, 345, 1052
AfaI GTAC 3 cut(s) 336, 591, 747
AfiI CCNNNNNNNGG 2 cut(s) 764, 1408
AgsI TTSAA 2 cut(s) 551, 1380
AluBI AGCT 8 cut(s) 67, 176, 287, 620, 884, 1187, 1238, 1429
AluI AGCT 8 cut(s) 67, 176, 287, 620, 884, 1187, 1238, 1429
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 3 cut(s) 125, 1208, 1412
AlwI GGATC 4 cut(s) 104, 436, 775, 1126
AlwNI CAGNNNCTG 1 cut(s) 967
AoxI GGCC 2 cut(s) 168, 963
ApeKI GCWGC 7 cut(s) 67, 620, 674, 863, 890, 1118, 1235
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 3 cut(s) 875, 964, 1388
AsuHPI GGTGA 1 cut(s) 1099
AsuNHI GCTAGC 1 cut(s) 412
AvaII GGWCC 2 cut(s) 875, 1388
BanII GRGCYC 1 cut(s) 158
BauI CACGAG 1 cut(s) 1239
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 523
BbvCI CCTCAGC 1 cut(s) 172
BbvI GCAGC 7 cut(s) 54, 607, 686, 850, 877, 1105, 1247
BccI CCATC 6 cut(s) 121, 445, 757, 1193, 1243, 1445
BciVI GTATCC 3 cut(s) 568, 779, 937
BcoDI GTCTC 3 cut(s) 125, 1208, 1412
BfaI CTAG 3 cut(s) 413, 599, 1164
BfmI CTRYAG 4 cut(s) 294, 525, 1092, 1119
BfoI RGCGCY 1 cut(s) 413
BfuAI ACCTGC 2 cut(s) 94, 346
BfuI GTATCC 3 cut(s) 568, 779, 937
BglII AGATCT 1 cut(s) 1139
BisI GCNGC 7 cut(s) 68, 621, 675, 864, 891, 1119, 1236
BlsI GCNGC 7 cut(s) 69, 622, 676, 865, 892, 1120, 1237
BmcAI AGTACT 2 cut(s) 591, 747
Bme18I GGWCC 2 cut(s) 875, 1388
BmgT120I GGNCC 3 cut(s) 875, 964, 1388
BmiI GGNNCC 1 cut(s) 1400
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 5 cut(s) 63, 402, 545, 931, 1105
BmtI GCTAGC 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpmI CTGGAG 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 172
BsaI GGTCTC 1 cut(s) 1208
BsaJI CCNNGG 3 cut(s) 562, 1110, 1391
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 764, 1408
Bse1I ACTGG 4 cut(s) 156, 587, 673, 878
Bse3DI GCAATG 4 cut(s) 126, 264, 684, 770
BseDI CCNNGG 3 cut(s) 562, 1110, 1391
BseGI GGATG 1 cut(s) 1120
BseLI CCNNNNNNNGG 2 cut(s) 764, 1408
BseMI GCAATG 4 cut(s) 126, 264, 684, 770
BseMII CTCAG 5 cut(s) 186, 247, 333, 1407, 1439
BseNI ACTGG 4 cut(s) 156, 587, 673, 878
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 7 cut(s) 54, 607, 686, 850, 877, 1105, 1247
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 170, 965
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 1 cut(s) 485
BslFI GGGAC 2 cut(s) 17, 1424
BslI CCNNNNNNNGG 2 cut(s) 764, 1408
BsmAI GTCTC 3 cut(s) 125, 1208, 1412
BsmFI GGGAC 2 cut(s) 17, 1424
BsnI GGCC 2 cut(s) 170, 965
Bso31I GGTCTC 1 cut(s) 1208
Bsp1286I GDGCHC 2 cut(s) 158, 523
Bsp143I GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
Bsp19I CCATGG 1 cut(s) 1391
BspANI GGCC 2 cut(s) 170, 965
BspCNI CTCAG 5 cut(s) 185, 246, 334, 1408, 1438
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 1400
BspMAI CTGCAG 2 cut(s) 529, 1123
BspMI ACCTGC 2 cut(s) 94, 346
BspOI GCTAGC 1 cut(s) 416
BspPI GGATC 4 cut(s) 104, 436, 775, 1126
BspQI GCTCTTC 1 cut(s) 552
BspTNI GGTCTC 1 cut(s) 1208
BsrDI GCAATG 4 cut(s) 126, 264, 684, 770
BsrI ACTGG 4 cut(s) 156, 587, 673, 878
BssECI CCNNGG 3 cut(s) 562, 1110, 1391
BssMI GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
BssNAI GTATAC 1 cut(s) 908
BssSI CACGAG 1 cut(s) 1239
BssT1I CCWWGG 2 cut(s) 1110, 1391
Bst1107I GTATAC 1 cut(s) 908
Bst2BI CACGAG 1 cut(s) 1239
Bst4CI ACNGT 3 cut(s) 11, 688, 706
Bst6I CTCTTC 2 cut(s) 552, 1340
BstAPI GCANNNNNTGC 3 cut(s) 275, 518, 1241
BstC8I GCNNGC 4 cut(s) 65, 414, 1346, 1350
BstDEI CTNAG 6 cut(s) 172, 233, 342, 1136, 1416, 1425
BstDSI CCRYGG 2 cut(s) 562, 1391
BstF5I GGATG 1 cut(s) 1120
BstFNI CGCG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 6 cut(s) 112, 431, 723, 770, 1134, 1142
BstMAI GTCTC 3 cut(s) 125, 1208, 1412
BstMBI GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
BstNSI RCATGY 1 cut(s) 403
BstSFI CTRYAG 4 cut(s) 294, 525, 1092, 1119
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 7 cut(s) 54, 607, 686, 850, 877, 1105, 1247
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 3 cut(s) 428, 1131, 1139
BstYI RGATCY 3 cut(s) 428, 1131, 1139
BstZ17I GTATAC 1 cut(s) 908
BsuI GTATCC 3 cut(s) 568, 779, 937
BsuRI GGCC 2 cut(s) 170, 965
BtgI CCRYGG 2 cut(s) 562, 1391
BtsCI GGATG 1 cut(s) 1120
BtsIMutI CAGTG 5 cut(s) 16, 241, 369, 1165, 1413
BveI ACCTGC 2 cut(s) 94, 346
Cac8I GCNNGC 4 cut(s) 65, 414, 1346, 1350
CaiI CAGNNNCTG 1 cut(s) 967
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 875, 964, 1388
Csp6I GTAC 3 cut(s) 335, 590, 746
CviAII CATG 6 cut(s) 400, 841, 1027, 1246, 1339, 1392
CviQI GTAC 3 cut(s) 335, 590, 746
DdeI CTNAG 6 cut(s) 172, 233, 342, 1136, 1416, 1425
DpnI GATC 6 cut(s) 111, 430, 722, 769, 1133, 1141
DpnII GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
DraI TTTAAA 1 cut(s) 732
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 552, 1340
EarI CTCTTC 2 cut(s) 552, 1340
Eco130I CCWWGG 2 cut(s) 1110, 1391
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 1208
Eco32I GATATC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 875, 1388
Eco57I CTGAAG 4 cut(s) 57, 279, 345, 1052
EcoO109I RGGNCCY 1 cut(s) 964
EcoRV GATATC 1 cut(s) 459
EcoT14I CCWWGG 2 cut(s) 1110, 1391
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 2 cut(s) 1110, 1391
FaeI CATG 6 cut(s) 403, 844, 1030, 1249, 1342, 1395
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 2 cut(s) 17, 1424
FatI CATG 6 cut(s) 399, 840, 1026, 1245, 1338, 1391
FauNDI CATATG 1 cut(s) 1231
FblI GTMKAC 3 cut(s) 148, 683, 907
Fnu4HI GCNGC 7 cut(s) 68, 621, 675, 864, 891, 1119, 1236
FokI GGATG 1 cut(s) 1127
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 7 cut(s) 68, 621, 675, 864, 891, 1119, 1236
FspBI CTAG 3 cut(s) 413, 599, 1164
GlaI GCGC 1 cut(s) 411
GluI GCNGC 7 cut(s) 68, 621, 675, 864, 891, 1119, 1236
GsuI CTGGAG 1 cut(s) 570
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 2 cut(s) 170, 965
HapII CCGG 1 cut(s) 485
HhaI GCGC 1 cut(s) 412
Hin1II CATG 6 cut(s) 403, 844, 1030, 1249, 1342, 1395
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HincII GTYRAC 2 cut(s) 369, 1169
HindII GTYRAC 2 cut(s) 369, 1169
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 2 cut(s) 4, 1330
HpaII CCGG 1 cut(s) 485
HphI GGTGA 1 cut(s) 1099
Hpy166II GTNNAC 6 cut(s) 149, 369, 684, 908, 1107, 1169
Hpy188I TCNGA 5 cut(s) 444, 900, 1222, 1370, 1442
Hpy188III TCNNGA 1 cut(s) 835
Hpy8I GTNNAC 6 cut(s) 149, 369, 684, 908, 1107, 1169
HpyAV CCTTC 2 cut(s) 941, 1302
HpyCH4III ACNGT 3 cut(s) 11, 688, 706
HpyF3I CTNAG 6 cut(s) 172, 233, 342, 1136, 1416, 1425
Hsp92II CATG 6 cut(s) 403, 844, 1030, 1249, 1342, 1395
HspAI GCGC 1 cut(s) 410
Kzo9I GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 2 cut(s) 526, 1294
Lsp1109I GCAGC 7 cut(s) 54, 607, 686, 850, 877, 1105, 1247
LweI GCATC 5 cut(s) 63, 402, 545, 931, 1105
MaeI CTAG 3 cut(s) 413, 599, 1164
MaeIII GTNAC 5 cut(s) 20, 630, 664, 706, 1432
MalI GATC 6 cut(s) 111, 430, 722, 769, 1133, 1141
MboI GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
MboII GAAGA 8 cut(s) 272, 323, 351, 539, 800, 828, 1045, 1327
MfeI CAATTG 2 cut(s) 90, 105
MflI RGATCY 3 cut(s) 428, 1131, 1139
MhlI GDGCHC 2 cut(s) 158, 523
MluCI AATT 8 cut(s) 90, 105, 199, 315, 353, 776, 1002, 1068
MlyI GAGTC 1 cut(s) 1324
MnlI CCTC 5 cut(s) 181, 237, 242, 400, 765
MseI TTAA 6 cut(s) 198, 219, 570, 716, 731, 1008
MslI CAYNNNNRTG 4 cut(s) 119, 398, 839, 1212
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 1 cut(s) 485
MunI CAATTG 2 cut(s) 90, 105
MvnI CGCG 1 cut(s) 63
NcoI CCATGG 1 cut(s) 1391
NdeI CATATG 1 cut(s) 1231
NdeII GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
NheI GCTAGC 1 cut(s) 412
NlaIII CATG 6 cut(s) 403, 844, 1030, 1249, 1342, 1395
NlaIV GGNNCC 1 cut(s) 1400
NmuCI GTSAC 2 cut(s) 20, 706
NspI RCATGY 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 7 cut(s) 69, 622, 676, 865, 892, 1120, 1237
PleI GAGTC 1 cut(s) 1324
PpsI GAGTC 1 cut(s) 1324
PspN4I GGNNCC 1 cut(s) 1400
PspPI GGNCC 3 cut(s) 875, 964, 1388
PstI CTGCAG 2 cut(s) 529, 1123
PstNI CAGNNNCTG 1 cut(s) 967
PsuI RGATCY 3 cut(s) 428, 1131, 1139
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 3 cut(s) 336, 591, 747
RsaNI GTAC 3 cut(s) 335, 590, 746
RseI CAYNNNNRTG 4 cut(s) 119, 398, 839, 1212
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 6 cut(s) 198, 219, 570, 716, 731, 1008
SatI GCNGC 7 cut(s) 68, 621, 675, 864, 891, 1119, 1236
Sau3AI GATC 6 cut(s) 109, 428, 720, 767, 1131, 1139
Sau96I GGNCC 3 cut(s) 875, 964, 1388
ScaI AGTACT 2 cut(s) 591, 747
SchI GAGTC 1 cut(s) 1324
SduI GDGCHC 2 cut(s) 158, 523
SfaNI GCATC 5 cut(s) 63, 402, 545, 931, 1105
SfcI CTRYAG 4 cut(s) 294, 525, 1092, 1119
SinI GGWCC 2 cut(s) 875, 1388
SmiMI CAYNNNNRTG 4 cut(s) 119, 398, 839, 1212
Sse9I AATT 8 cut(s) 90, 105, 199, 315, 353, 776, 1002, 1068
SspMI CTAG 3 cut(s) 413, 599, 1164
StyI CCWWGG 2 cut(s) 1110, 1391
TaaI ACNGT 3 cut(s) 11, 688, 706
TaqI TCGA 1 cut(s) 1197
TaqII GACCGA 1 cut(s) 1292
TasI AATT 8 cut(s) 90, 105, 199, 315, 353, 776, 1002, 1068
TatI WGTACW 2 cut(s) 589, 745
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 6 cut(s) 198, 219, 570, 716, 731, 1008
Tru9I TTAA 6 cut(s) 198, 219, 570, 716, 731, 1008
TscAI CASTG 5 cut(s) 16, 241, 369, 1165, 1420
TseFI GTSAC 2 cut(s) 20, 706
TseI GCWGC 7 cut(s) 67, 620, 674, 863, 890, 1118, 1235
Tsp45I GTSAC 2 cut(s) 20, 706
TspDTI ATGAA 7 cut(s) 17, 203, 324, 972, 1015, 1047, 1327
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 5 cut(s) 16, 241, 369, 1165, 1420
VpaK11BI GGWCC 2 cut(s) 875, 1388
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 1252
XmiI GTMKAC 3 cut(s) 148, 683, 907
XspI CTAG 3 cut(s) 413, 599, 1164
ZrmI AGTACT 2 cut(s) 591, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.