Rw1G013260

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001524
Physical Location & Seq
Forward (+)
29552654 .. 29554222
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G013260.1

Sequence Viewer

Length: 576 bp
ATGAAAATGACGGCTACCCAATTTGGAGGTGGTGCACCTTCTACCGGGTTGAGGAATAGAACCAGTGACTTCAATTCAGTAACTAAGAAAGATTGGGTTTATTCATTGCGGCAAACGGAAGCTAAGTTTGTGGCACTAAGCAATAGAAAGGATTTGAGCAATGTCAGTGCTGTGGGGGGCAGCAATCTGTCATCTAAGCATAGATCACAGTACTCGCGGCTAGTTGCTCATCGCAGAAGGACCCGTTTTCCGGTGGTGGCTAATCAATTGAGTTCGGAGTTTGGTGTCGGTTGCTCTGAGGTTGAGGAAAAGGTGATTCTTAAGGAAATGCATGCTCCCCTAACTGCTGATCGTGATGAACCTGAGTTGAGTGACATTCCTGTCCCTGAAGCATATTCTCCAAATGTGAAACGCGAGCTGCTGATGCTTTCCTTACCTGCAATTGTGGGTCAGGCAATTGATCCCATAGCAATGCTGATGGAGACTGCTTATATTGGTAGACTGGGGTCATTTTGGGAATTTCATATGCGTGTGGGTCCCACTTTTGCTGAGTTGGAATGCCACGTCAGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

191

Amino Acids

21.17

Weight (kDa)

8.58

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 380
Acc36I ACCTGC 1 cut(s) 445
AccI GTMKAC 1 cut(s) 499
AccII CGCG 2 cut(s) 217, 414
AciI CCGC 2 cut(s) 109, 217
AclWI GGATC 1 cut(s) 455
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 44, 51, 250
AflII CTTAAG 1 cut(s) 320
AgsI TTSAA 1 cut(s) 73
AjiI CACGTC 1 cut(s) 565
AluBI AGCT 2 cut(s) 122, 418
AluI AGCT 2 cut(s) 122, 418
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 476
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 1 cut(s) 455
ApaLI GTGCAC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 180, 418
ApoI RAATTY 1 cut(s) 518
AspS9I GGNCC 2 cut(s) 240, 536
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 325
AvaII GGWCC 2 cut(s) 240, 536
BaeGI GKGCMC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 192, 405
BccI CCATC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 27
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 1 cut(s) 221
BfrI CTTAAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 445
BisI GCNGC 4 cut(s) 110, 181, 218, 419
BlsI GCNGC 4 cut(s) 111, 182, 219, 420
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 240, 536
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 240, 536
BmiI GGNNCC 3 cut(s) 242, 537, 538
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 1 cut(s) 414
BmuI ACTGGG 1 cut(s) 512
BoxI GACNNNNGTC 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 51, 250
Bse1I ACTGG 2 cut(s) 63, 507
Bse3DI GCAATG 3 cut(s) 104, 166, 477
BseLI CCNNNNNNNGG 3 cut(s) 44, 51, 250
BseMI GCAATG 3 cut(s) 104, 166, 477
BseMII CTCAG 3 cut(s) 288, 354, 540
BseNI ACTGG 2 cut(s) 63, 507
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 192, 405
Bsh1236I CGCG 2 cut(s) 217, 414
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 2 cut(s) 45, 251
BslFI GGGAC 2 cut(s) 368, 522
BslI CCNNNNNNNGG 3 cut(s) 44, 51, 250
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 368, 522
BsmI GAATGC 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 3 cut(s) 203, 349, 460
BspACI CCGC 2 cut(s) 109, 217
BspCNI CTCAG 3 cut(s) 289, 355, 541
BspFNI CGCG 2 cut(s) 217, 414
BspLI GGNNCC 3 cut(s) 242, 537, 538
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 455
BspTI CTTAAG 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 104, 166, 477
BsrI ACTGG 2 cut(s) 63, 507
BssMI GATC 3 cut(s) 203, 349, 460
Bst4CI ACNGT 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 333, 416
BstDEI CTNAG 7 cut(s) 84, 123, 137, 195, 297, 363, 549
BstFNI CGCG 2 cut(s) 217, 414
BstKTI GATC 3 cut(s) 206, 352, 463
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 203, 349, 460
BstMWI GCNNNNNNNGC 2 cut(s) 424, 567
BstNSI RCATGY 1 cut(s) 335
BstPAI GACNNNNGTC 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 44
BstSLI GKGCMC 1 cut(s) 37
BstUI CGCG 2 cut(s) 217, 414
BstV1I GCAGC 2 cut(s) 192, 405
BtgZI GCGATG 1 cut(s) 215
BtrI CACGTC 1 cut(s) 565
BtsIMutI CAGTG 2 cut(s) 70, 172
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 2 cut(s) 333, 416
Cfr13I GGNCC 2 cut(s) 240, 536
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 332
CviJI RGCY 5 cut(s) 14, 122, 220, 260, 418
CviKI_1 RGCY 5 cut(s) 14, 122, 220, 260, 418
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 7 cut(s) 84, 123, 137, 195, 297, 363, 549
DpnI GATC 3 cut(s) 205, 351, 462
DpnII GATC 3 cut(s) 203, 349, 460
DrdI GACNNNNNNGTC 1 cut(s) 380
DseDI GACNNNNNNGTC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 240, 536
Eco57I CTGAAG 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 240, 536
EcoT22I ATGCAT 1 cut(s) 333
FaeI CATG 1 cut(s) 335
FaiI YATR 7 cut(s) 201, 333, 394, 467, 492, 525, 527
FaqI GGGAC 2 cut(s) 368, 522
FatI CATG 1 cut(s) 331
FauNDI CATATG 1 cut(s) 525
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 4 cut(s) 110, 181, 218, 419
Fsp4HI GCNGC 4 cut(s) 110, 181, 218, 419
FspBI CTAG 1 cut(s) 221
GluI GCNGC 4 cut(s) 110, 181, 218, 419
HapII CCGG 2 cut(s) 45, 251
Hin1II CATG 1 cut(s) 335
HinfI GANTC 1 cut(s) 316
HpaII CCGG 2 cut(s) 45, 251
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 35, 500
Hpy188I TCNGA 2 cut(s) 277, 298
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 2 cut(s) 35, 500
HpyAV CCTTC 2 cut(s) 48, 231
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 3 cut(s) 35, 331, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 567
HpyF3I CTNAG 7 cut(s) 84, 123, 137, 195, 297, 363, 549
HpySE526I ACGT 1 cut(s) 564
Hsp92II CATG 1 cut(s) 335
KflI GGGWCCC 1 cut(s) 536
Kzo9I GATC 3 cut(s) 203, 349, 460
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 9 cut(s) 58, 76, 264, 375, 393, 399, 437, 450, 488
Lsp1109I GCAGC 2 cut(s) 192, 405
LweI GCATC 1 cut(s) 414
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 3 cut(s) 65, 79, 371
MalI GATC 3 cut(s) 205, 351, 462
MboI GATC 3 cut(s) 203, 349, 460
MfeI CAATTG 3 cut(s) 266, 441, 456
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 6 cut(s) 20, 73, 266, 441, 456, 518
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 4 cut(s) 20, 45, 292, 298
Mph1103I ATGCAT 1 cut(s) 333
MseI TTAA 2 cut(s) 321, 574
MslI CAYNNNNRTG 2 cut(s) 470, 528
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 2 cut(s) 45, 251
MspR9I CCNGG 1 cut(s) 46
MunI CAATTG 3 cut(s) 266, 441, 456
Mva1269I GAATGC 1 cut(s) 563
MvnI CGCG 2 cut(s) 217, 414
MwoI GCNNNNNNNGC 2 cut(s) 424, 567
NciI CCSGG 1 cut(s) 46
NdeI CATATG 1 cut(s) 525
NdeII GATC 3 cut(s) 203, 349, 460
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 3 cut(s) 242, 537, 538
NmuCI GTSAC 2 cut(s) 65, 371
NsiI ATGCAT 1 cut(s) 333
NspI RCATGY 1 cut(s) 335
PaeI GCATGC 1 cut(s) 335
PctI GAATGC 1 cut(s) 563
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 4 cut(s) 111, 182, 219, 420
PpuMI RGGWCCY 2 cut(s) 240, 536
PshAI GACNNNNGTC 1 cut(s) 505
Psp5II RGGWCCY 2 cut(s) 240, 536
PspN4I GGNNCC 3 cut(s) 242, 537, 538
PspPI GGNCC 2 cut(s) 240, 536
PspPPI RGGWCCY 2 cut(s) 240, 536
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 470, 528
SaqAI TTAA 2 cut(s) 321, 574
SatI GCNGC 4 cut(s) 110, 181, 218, 419
Sau3AI GATC 3 cut(s) 203, 349, 460
Sau96I GGNCC 2 cut(s) 240, 536
ScaI AGTACT 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 37
SetI ASST 9 cut(s) 31, 40, 124, 303, 315, 364, 420, 439, 567
SfaNI GCATC 1 cut(s) 414
SinI GGWCC 2 cut(s) 240, 536
SmiMI CAYNNNNRTG 2 cut(s) 470, 528
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 1 cut(s) 335
Sse9I AATT 6 cut(s) 20, 73, 266, 441, 456, 518
SsiI CCGC 2 cut(s) 109, 217
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 1 cut(s) 567
TasI AATT 6 cut(s) 20, 73, 266, 441, 456, 518
TatI WGTACW 1 cut(s) 210
TauI GCSGC 2 cut(s) 112, 220
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 2 cut(s) 321, 574
Tru9I TTAA 2 cut(s) 321, 574
TscAI CASTG 2 cut(s) 70, 172
TseFI GTSAC 2 cut(s) 65, 371
TseI GCWGC 2 cut(s) 180, 418
Tsp45I GTSAC 2 cut(s) 65, 371
TspDTI ATGAA 4 cut(s) 17, 93, 372, 512
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 70, 172
Vha464I CTTAAG 1 cut(s) 320
VneI GTGCAC 1 cut(s) 33
VpaK11BI GGWCC 2 cut(s) 240, 536
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 1 cut(s) 221
ZrmI AGTACT 1 cut(s) 212
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.