pycom05g13020

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001524
Physical Location & Seq
Forward (+)
16554867 .. 16558059
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13020.4

Sequence Viewer

Length: 1194 bp
ATGTTAAGCATTGTTTTTTGTTTGTTTTGGGTTAATGAGTTGAATAACATTTCTATCAGTGAAACATATTCTCCAGATGTCAAACGCGAGCTATTGATGCTTTCTTTACCTGCAATTCTGGGTCAGGCCATTGATCCATTAGCACAACTAATGGAGACTGCATACATTGGCAATTTGGGTTTGCTTCACTCCTTTAAACGTTCCGTGGAATTGGCTTCAGCTGGTATCTCAATGAACATCTTTAATTATATCTCAAAGATATTTAATATCCCTCTCCTCAGTGTTGCTACATCTTTTGTTGCTGAAGACCTTGCTAAGAGTGAAAGTAAAGGTTCTACTTCAGATTTCTTAAAACCTTTTTTTTTTGGGAATCTGATTTTGGCTGATTTTCTTACAGAAAATGGTTATCTAGACGGCAAAACTAATGGTAAACCCAAACCTGTTGATGGAGTCGTTGAGAGAAAGCAGCTATCTTCAGTGTCTACAGCTTTATTGTTAGCAGTTGGGATTGGAATCTTTGAGGCCGTGGCCCTGTCTTTGGGATCTGGGTTGTTTCTAAATATGATGGGCGTATCATCAGTAGGTATTGGTAATTCGTTAGCTGTGTTTTTGTTGCCCCTCCTTATTTATTATTTTCGTTTGGGTGCAACTGGTGCAGCCATTTCCACTGTTATATCTCAATACACTGTCACTTTTTTAATGATCTGGTTTCTAAATAAGAGAGCTATACTATTGCCTCCGAATGATGATCGATCACTACAATTTGGTGGATACCTTAAATCCGGTGGCTTTCTTCTTGGACTAACTATTGCTTGTCTTACGACCTTGACATTGGGAACATCAATGGCGGCTCGTCAAGTATGGTTGGCTGTATCCCTTTTAACCGATGCAATGGCTGCATCTGGTCAGACCCTGATTGCAAGTTATTTATCAAAAGGTGAATACAAAATTGTGAAAGAAGTTGCTGACTCTGTGCTAAAGATAGGATTGTTCACAGTTATTTCCTTGTCTGTAATTTTGGGAGTATCTTTTGGTTCTCTAGCTACGTTGTTCACCAACGATCCTGAAGTATTAGCAATTGATCGATCTGGGATATTGCTATATGCTCCTTCCATTTTTGGCTTTCATGGAGTTTGGGTTGGTTTGACTCTCTTCATGGGCTTGCGCGCAGTCGCAGGCTATGCCAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

398

Amino Acids

42.56

Weight (kDa)

6.75

Isoelectric Point (pI)

27.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 482
AccII CGCG 2 cut(s) 87, 1167
AciI CCGC 1 cut(s) 848
AclI AACGTT 1 cut(s) 199
AclWI GGATC 3 cut(s) 128, 550, 1055
AcsI RAATTY 1 cut(s) 1187
AcuI CTGAAG 5 cut(s) 201, 324, 324, 459, 1086
AfiI CCNNNNNNNGG 2 cut(s) 446, 538
AgsI TTSAA 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 7 cut(s) 91, 221, 469, 488, 602, 725, 1043
AluI AGCT 7 cut(s) 91, 221, 469, 488, 602, 725, 1043
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 3 cut(s) 128, 550, 1055
AlwNI CAGNNNCTG 1 cut(s) 913
AoxI GGCC 3 cut(s) 126, 522, 528
ApeKI GCWGC 3 cut(s) 466, 656, 896
ApoI RAATTY 1 cut(s) 1187
AspLEI GCGC 2 cut(s) 1167, 1169
AspS9I GGNCC 1 cut(s) 529
AsuHPI GGTGA 2 cut(s) 950, 1045
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 3 cut(s) 478, 668, 883
BccI CCATC 2 cut(s) 440, 559
BceAI ACGGC 2 cut(s) 430, 509
BciVI GTATCC 2 cut(s) 764, 883
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 2 cut(s) 410, 1040
BfmI CTRYAG 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 118
BfuI GTATCC 2 cut(s) 764, 883
BisI GCNGC 4 cut(s) 467, 657, 849, 897
BlsI GCNGC 4 cut(s) 468, 658, 850, 898
BmgT120I GGNCC 1 cut(s) 529
BmsI GCATC 3 cut(s) 87, 877, 908
BpiI GAAGAC 1 cut(s) 312
BpmI CTGGAG 1 cut(s) 57
Bsa29I ATCGAT 2 cut(s) 751, 1084
BsaBI GATNNNNATC 1 cut(s) 512
BsaJI CCNNGG 2 cut(s) 204, 525
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 1122, 1152
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 538
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 512
BseCI ATCGAT 2 cut(s) 751, 1084
BseDI CCNNGG 2 cut(s) 204, 525
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 2 cut(s) 446, 538
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 655
BsePI GCGCGC 1 cut(s) 1165
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 478, 668, 883
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 2 cut(s) 87, 1167
BshFI GGCC 3 cut(s) 128, 524, 530
BshVI ATCGAT 2 cut(s) 751, 1084
BsiSI CCGG 1 cut(s) 783
BslI CCNNNNNNNGG 2 cut(s) 446, 538
BsmAI GTCTC 1 cut(s) 149
BsnI GGCC 3 cut(s) 128, 524, 530
Bsp143I GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
BspACI CCGC 1 cut(s) 848
BspANI GGCC 3 cut(s) 128, 524, 530
BspCNI CTCAG 1 cut(s) 291
BspDI ATCGAT 2 cut(s) 751, 1084
BspFNI CGCG 2 cut(s) 87, 1167
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 3 cut(s) 128, 550, 1055
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 1 cut(s) 655
BssECI CCNNGG 2 cut(s) 204, 525
BssHII GCGCGC 1 cut(s) 1165
BssMI GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
Bst4CI ACNGT 3 cut(s) 670, 688, 997
Bst6I CTCTTC 1 cut(s) 1157
BstAPI GCANNNNNTGC 3 cut(s) 653, 896, 1181
BstC8I GCNNGC 4 cut(s) 89, 1163, 1167, 1177
BstDEI CTNAG 2 cut(s) 278, 315
BstDSI CCRYGG 2 cut(s) 204, 525
BstFNI CGCG 2 cut(s) 87, 1167
BstHHI GCGC 2 cut(s) 1167, 1169
BstKTI GATC 8 cut(s) 136, 545, 705, 751, 755, 1063, 1084, 1088
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
BstMWI GCNNNNNNNGC 4 cut(s) 97, 653, 896, 1181
BstSFI CTRYAG 1 cut(s) 483
BstUI CGCG 2 cut(s) 87, 1167
BstV1I GCAGC 3 cut(s) 478, 668, 883
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
Bsu15I ATCGAT 2 cut(s) 751, 1084
BsuI GTATCC 2 cut(s) 764, 883
BsuRI GGCC 3 cut(s) 128, 524, 530
BsuTUI ATCGAT 2 cut(s) 751, 1084
BtgI CCRYGG 2 cut(s) 204, 525
BtsIMutI CAGTG 5 cut(s) 64, 286, 483, 666, 684
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 4 cut(s) 89, 1163, 1167, 1177
CaiI CAGNNNCTG 1 cut(s) 913
CfoI GCGC 2 cut(s) 1167, 1169
Cfr13I GGNCC 1 cut(s) 529
ClaI ATCGAT 2 cut(s) 751, 1084
CviAII CATG 2 cut(s) 1127, 1156
DdeI CTNAG 2 cut(s) 278, 315
DpnI GATC 8 cut(s) 135, 544, 704, 750, 754, 1062, 1083, 1087
DpnII GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
DraI TTTAAA 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 1157
EarI CTCTTC 1 cut(s) 1157
Eco57I CTGAAG 5 cut(s) 201, 324, 324, 459, 1086
FaeI CATG 2 cut(s) 1130, 1159
FatI CATG 2 cut(s) 1126, 1155
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 4 cut(s) 467, 657, 849, 897
Fsp4HI GCNGC 4 cut(s) 467, 657, 849, 897
FspBI CTAG 2 cut(s) 410, 1040
GlaI GCGC 2 cut(s) 1166, 1168
GluI GCNGC 4 cut(s) 467, 657, 849, 897
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 3 cut(s) 128, 524, 530
HapII CCGG 1 cut(s) 783
HhaI GCGC 2 cut(s) 1167, 1169
Hin1II CATG 2 cut(s) 1130, 1159
Hin6I GCGC 2 cut(s) 1165, 1167
HinP1I GCGC 2 cut(s) 1165, 1167
HinfI GANTC 5 cut(s) 370, 450, 513, 968, 1147
HpaII CCGG 1 cut(s) 783
HphI GGTGA 2 cut(s) 950, 1045
Hpy166II GTNNAC 4 cut(s) 431, 483, 993, 1053
Hpy188I TCNGA 4 cut(s) 343, 375, 741, 909
Hpy188III TCNNGA 3 cut(s) 74, 410, 1064
Hpy8I GTNNAC 4 cut(s) 431, 483, 993, 1053
HpyAV CCTTC 1 cut(s) 1119
HpyCH4III ACNGT 3 cut(s) 670, 688, 997
HpyCH4IV ACGT 2 cut(s) 199, 1046
HpyCH4V TGCA 7 cut(s) 113, 161, 647, 656, 890, 899, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 653, 896, 1181
HpyF3I CTNAG 2 cut(s) 278, 315
HpySE526I ACGT 2 cut(s) 199, 1046
Hsp92II CATG 2 cut(s) 1130, 1159
HspAI GCGC 2 cut(s) 1165, 1167
Kzo9I GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
LmnI GCTCC 1 cut(s) 1111
Lsp1109I GCAGC 3 cut(s) 478, 668, 883
LweI GCATC 3 cut(s) 87, 877, 908
MaeI CTAG 2 cut(s) 410, 1040
MaeII ACGT 2 cut(s) 199, 1046
MaeIII GTNAC 1 cut(s) 688
MalI GATC 8 cut(s) 135, 544, 704, 750, 754, 1062, 1083, 1087
MboI GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
MboII GAAGA 4 cut(s) 317, 465, 785, 1144
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 1 cut(s) 542
MlyI GAGTC 3 cut(s) 459, 962, 1141
MnlI CCTC 5 cut(s) 282, 287, 514, 629, 747
MseI TTAA 9 cut(s) 5, 33, 195, 243, 264, 350, 698, 777, 881
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 783
MunI CAATTG 1 cut(s) 1077
MvnI CGCG 2 cut(s) 87, 1167
MwoI GCNNNNNNNGC 4 cut(s) 97, 653, 896, 1181
NdeII GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
NlaIII CATG 2 cut(s) 1130, 1159
NmuCI GTSAC 1 cut(s) 688
PauI GCGCGC 1 cut(s) 1165
PfeI GAWTC 2 cut(s) 370, 513
PkrI GCNGC 4 cut(s) 468, 658, 850, 898
PleI GAGTC 3 cut(s) 458, 962, 1141
PpsI GAGTC 3 cut(s) 458, 962, 1141
Psp1406I AACGTT 1 cut(s) 199
PspPI GGNCC 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 913
PsuI RGATCY 1 cut(s) 542
PteI GCGCGC 1 cut(s) 1165
PvuII CAGCTG 1 cut(s) 221
SaqAI TTAA 9 cut(s) 5, 33, 195, 243, 264, 350, 698, 777, 881
SatI GCNGC 4 cut(s) 467, 657, 849, 897
Sau3AI GATC 8 cut(s) 133, 542, 702, 748, 752, 1060, 1081, 1085
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 3 cut(s) 459, 962, 1141
SfaNI GCATC 3 cut(s) 87, 877, 908
SfcI CTRYAG 1 cut(s) 483
SsiI CCGC 1 cut(s) 848
SspMI CTAG 2 cut(s) 410, 1040
TaaI ACNGT 3 cut(s) 670, 688, 997
TaiI ACGT 2 cut(s) 202, 1049
TaqI TCGA 2 cut(s) 751, 1084
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 2 cut(s) 370, 513
Tru1I TTAA 9 cut(s) 5, 33, 195, 243, 264, 350, 698, 777, 881
Tru9I TTAA 9 cut(s) 5, 33, 195, 243, 264, 350, 698, 777, 881
TscAI CASTG 5 cut(s) 64, 286, 483, 673, 691
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 3 cut(s) 466, 656, 896
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 3 cut(s) 248, 1115, 1144
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 5 cut(s) 64, 286, 483, 673, 691
XapI RAATTY 1 cut(s) 1187
XbaI TCTAGA 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 482
XspI CTAG 2 cut(s) 410, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.