Rorug06G0130500

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001524
Physical Location & Seq
Reverse (-)
18207137 .. 18209731
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0130500.1

Sequence Viewer

Length: 630 bp
ATGAACTGTGTAGCAACAACTGCGAGAGCTTTTGTGACTGTGAAAGCCTCCAACCCCAATTTCAGAACTTTCCTTCTTCCTCTTCACTCTCGCAGGTCCCTGTGTCGTGTTAGCGCTACAAACAATGAAGAGGCTGCTGCCAAGGTAGCTGCAGCAAATGCTGACAGAGGAGCTCCAACAATATTTGACAAGATCATAGCAAAGGAAATCCCATCGACCATTGTATACGAGGATGATAAGGTCCTGGCATTTCGGGATATCAACCCACAGGCTCCTGTGCATGTTGTTATCATCCCAAAGAATAGGGATGGGTTAACAGAGCTGGGAAAGAAGAAAAAAGGCTTTCTTTTGATCTATGCATTCTGTCATTGCAGGCTGAATCTAGGCATGTGGAAATATTGGGTCAACTTCTATATGCTGCAAAAAAAGTTGCTGAGAAAGAAGGTATTCTTCACGGGCAGAGCAAATACAAAAGACAGAGCAAATACGATGGGTATGTGGCATGGTTCTTATGATAAATTGGATAATGATTTGCACATCCTGTTATATTACAAGACTACTGGGAGAAGCGGAGGATTTGGGTTTGCGACCATGTCTAGTGATCAGGAAGTAGAAGCAGCTGCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

209

Amino Acids

23.56

Weight (kDa)

9.79

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DcpS_C PF11969 60 - 101 1.7e-10 Scavenger mRNA decapping enzyme C-term binding
HIT PF01230 68 - 101 3.2e-10 HIT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 84
AccI GTMKAC 1 cut(s) 225
AciI CCGC 1 cut(s) 570
AfeI AGCGCT 1 cut(s) 115
AjnI CCWGG 1 cut(s) 243
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 5 cut(s) 29, 149, 173, 322, 620
AluI AGCT 5 cut(s) 29, 149, 173, 322, 620
Alw21I GWGCWC 1 cut(s) 175
Aor51HI AGCGCT 1 cut(s) 115
ApeKI GCWGC 7 cut(s) 134, 137, 149, 152, 418, 617, 620
Asp700I GAANNNNTTC 1 cut(s) 446
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 96, 241
AvaII GGWCC 2 cut(s) 96, 241
BanII GRGCYC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 6 cut(s) 121, 124, 136, 164, 405, 607
BccI CCATC 3 cut(s) 220, 302, 484
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 1 cut(s) 601
BfaI CTAG 2 cut(s) 383, 597
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 84
BisI GCNGC 7 cut(s) 135, 138, 150, 153, 419, 618, 621
BlsI GCNGC 7 cut(s) 136, 139, 151, 154, 420, 619, 622
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 2 cut(s) 96, 241
BmgT120I GGNCC 2 cut(s) 96, 241
BmiI GGNNCC 2 cut(s) 98, 273
BmrFI CCNGG 1 cut(s) 245
BmrI ACTGGG 1 cut(s) 570
BmuI ACTGGG 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 4 cut(s) 238, 291, 313, 537
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 6 cut(s) 121, 124, 136, 164, 405, 607
BseYI CCCAGC 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 175
BslFI GGGAC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 3 cut(s) 192, 351, 601
BspACI CCGC 1 cut(s) 570
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 98, 273
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 367
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 3 cut(s) 192, 351, 601
BssNAI GTATAC 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 2 cut(s) 8, 40
Bst6I CTCTTC 2 cut(s) 87, 123
BstAPI GCANNNNNTGC 2 cut(s) 20, 158
BstC8I GCNNGC 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 434
BstF5I GGATG 4 cut(s) 238, 291, 313, 537
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 195, 354, 604
BstMBI GATC 3 cut(s) 192, 351, 601
BstMWI GCNNNNNNNGC 3 cut(s) 20, 146, 158
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 2 cut(s) 284, 391
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 6 cut(s) 121, 124, 136, 164, 405, 607
BstZ17I GTATAC 1 cut(s) 226
BtsCI GGATG 4 cut(s) 238, 291, 313, 537
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 374
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 96, 241
CviAII CATG 4 cut(s) 281, 388, 503, 592
DdeI CTNAG 1 cut(s) 434
DpnI GATC 3 cut(s) 194, 353, 603
DpnII GATC 3 cut(s) 192, 351, 601
Eam1104I CTCTTC 2 cut(s) 87, 123
EarI CTCTTC 2 cut(s) 87, 123
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 96, 241
Eco47III AGCGCT 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 173
EcoO109I RGGNCCY 2 cut(s) 96, 241
EcoRII CCWGG 1 cut(s) 243
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 4 cut(s) 284, 391, 506, 595
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 434, 466
FaqI GGGAC 1 cut(s) 82
FatI CATG 4 cut(s) 280, 387, 502, 591
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 7 cut(s) 135, 138, 150, 153, 419, 618, 621
FokI GGATG 4 cut(s) 245, 278, 320, 524
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 7 cut(s) 135, 138, 150, 153, 419, 618, 621
FspBI CTAG 2 cut(s) 383, 597
GlaI GCGC 1 cut(s) 115
GluI GCNGC 7 cut(s) 135, 138, 150, 153, 419, 618, 621
GsaI CCCAGC 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 117
HhaI GCGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 284, 391, 506, 595
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 315, 406
HindII GTYRAC 2 cut(s) 315, 406
HinfI GANTC 1 cut(s) 379
HpaI GTTAAC 1 cut(s) 315
Hpy166II GTNNAC 3 cut(s) 226, 315, 406
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 2 cut(s) 254, 605
Hpy8I GTNNAC 3 cut(s) 226, 315, 406
HpyAV CCTTC 2 cut(s) 83, 436
HpyCH4III ACNGT 2 cut(s) 8, 40
HpyCH4V TGCA 6 cut(s) 152, 280, 359, 372, 421, 535
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 146, 158
HpyF3I CTNAG 1 cut(s) 434
Hsp92II CATG 4 cut(s) 284, 391, 506, 595
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 601
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 3 cut(s) 192, 351, 601
LmnI GCTCC 3 cut(s) 170, 178, 277
Lsp1109I GCAGC 6 cut(s) 121, 124, 136, 164, 405, 607
MaeI CTAG 2 cut(s) 383, 597
MaeIII GTNAC 1 cut(s) 34
MalI GATC 3 cut(s) 194, 353, 603
MboI GATC 3 cut(s) 192, 351, 601
MboII GAAGA 5 cut(s) 68, 74, 140, 343, 442
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 2 cut(s) 58, 518
MmeI TCCRAC 2 cut(s) 75, 200
MnlI CCTC 6 cut(s) 58, 90, 124, 161, 223, 566
Mph1103I ATGCAT 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 620
MspR9I CCNGG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 20, 146, 158
NdeII GATC 3 cut(s) 192, 351, 601
NlaIII CATG 4 cut(s) 284, 391, 506, 595
NlaIV GGNNCC 2 cut(s) 98, 273
NmuCI GTSAC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 2 cut(s) 284, 391
PctI GAATGC 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 379
PflFI GACNNNGTC 1 cut(s) 592
PkrI GCNGC 7 cut(s) 136, 139, 151, 154, 420, 619, 622
PpuMI RGGWCCY 2 cut(s) 96, 241
Psp124BI GAGCTC 1 cut(s) 175
Psp5II RGGWCCY 2 cut(s) 96, 241
Psp6I CCWGG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 98, 273
PspPI GGNCC 2 cut(s) 96, 241
PspPPI RGGWCCY 2 cut(s) 96, 241
PstI CTGCAG 1 cut(s) 154
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 7 cut(s) 135, 138, 150, 153, 419, 618, 621
Sau3AI GATC 3 cut(s) 192, 351, 601
Sau96I GGNCC 2 cut(s) 96, 241
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 175
SetI ASST 9 cut(s) 31, 98, 147, 151, 175, 243, 324, 447, 622
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 2 cut(s) 96, 241
Sse9I AATT 2 cut(s) 58, 518
SsiI CCGC 1 cut(s) 570
SspI AATATT 2 cut(s) 183, 398
SspMI CTAG 2 cut(s) 383, 597
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 8, 40
TaqI TCGA 2 cut(s) 215, 625
TasI AATT 2 cut(s) 58, 518
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 7 cut(s) 134, 137, 149, 152, 418, 617, 620
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 17, 141
Tth111I GACNNNGTC 1 cut(s) 592
VpaK11BI GGWCC 2 cut(s) 96, 241
XceI RCATGY 2 cut(s) 284, 391
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 2 cut(s) 383, 597
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.