pycom05g13000

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001524
Physical Location & Seq
Forward (+)
16532285 .. 16533970
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13000.4

Sequence Viewer

Length: 789 bp
ATGGCGGTTACCCAGTTCAGAAGTGGCGCCCTTTCTAACGGATTAACCGCCGGAGCCAGTGACCGCAACCCGATAACTCGAAAGTCCAGGTTGTTTAATTCATTGCACAGAAGGGGAGATTGTAATATTGTAGCAAGAAGTGGTAGGAAGGATTTGAGCAATGCCAATGCTGTGGGGGGATACAATCTGTATGCTACACGAAGAGCACTGTGCACGCCGCTAGTGACACGCCGCAGAAGACCCTGTTTCCCGGTGGTGGCTAATCAATTGAGTTCGGATTTGGGTGTGGGATCGTCTGAGGTGGAAGGAAAGTTGGGTTTGGAGGAAGAGGAAGCTCTCATAGGTGTAGGGAGTGGTGACCTTGAAACATATTCTCCAGATGTCAAACGCGAGCTATTGATGCTTTCTTTACCTGCAATTCTGGGTCAGGCCATCGATCCATTAGCACAACTAATGGAGACTGCATACATTGGCAATTTGGGTTCTGTGGAATTGGCTTCAGCTGGTATTTCAATGAACATCTTTAATTATATCTCAAAGATATTTAATATCCCTCTCCTCAGTGTTGCGACATCTTTTGTTGCTGAAGACCTTGTTAAGAGTGAAAGTAAAGGTTCTATTTCAGAAAATGGTTGTCTAAATGGCAATACTAATGGTAAACTCAGAACTGTTGATGGAGTTGTTGAGAGAAAGCAGCTGTCTTCAGTGTCTACAGCTTTATTGTTAGCAGTTGGGATTGGGATTTTTGAGGCCGTGGCCCTGTCTTTGGGATCGATGGGTTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

263

Amino Acids

27.67

Weight (kDa)

8.28

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 710
AccII CGCG 1 cut(s) 390
AciI CCGC 5 cut(s) 5, 48, 64, 218, 232
AclWI GGATC 3 cut(s) 298, 431, 778
AcuI CTGAAG 3 cut(s) 483, 606, 687
AcyI GRCGYC 1 cut(s) 27
AfiI CCNNNNNNNGG 2 cut(s) 256, 766
AgsI TTSAA 2 cut(s) 365, 513
AjnI CCWGG 1 cut(s) 86
AluBI AGCT 5 cut(s) 335, 394, 503, 697, 716
AluI AGCT 5 cut(s) 335, 394, 503, 697, 716
Alw21I GWGCWC 2 cut(s) 208, 215
Alw26I GTCTC 1 cut(s) 452
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 3 cut(s) 298, 431, 778
AoxI GGCC 3 cut(s) 429, 750, 756
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 694
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 26
BbsI GAAGAC 3 cut(s) 244, 594, 693
Bbv12I GWGCWC 2 cut(s) 208, 215
BbvI GCAGC 1 cut(s) 706
BccI CCATC 3 cut(s) 440, 668, 769
BceAI ACGGC 1 cut(s) 737
BciT130I CCWGG 1 cut(s) 88
BciVI GTATCC 1 cut(s) 173
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 1 cut(s) 30
BfuAI ACCTGC 1 cut(s) 421
BfuI GTATCC 1 cut(s) 173
BisI GCNGC 3 cut(s) 218, 232, 695
BlsI GCNGC 3 cut(s) 219, 233, 696
Bme1390I CCNGG 2 cut(s) 88, 251
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 2 cut(s) 28, 55
BmrFI CCNGG 2 cut(s) 88, 251
BmrI ACTGGG 1 cut(s) 7
BmsI GCATC 1 cut(s) 390
BmuI ACTGGG 1 cut(s) 7
BpiI GAAGAC 3 cut(s) 244, 594, 693
BpmI CTGGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 251
Bsa29I ATCGAT 2 cut(s) 435, 773
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 753
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 766
Bse1I ACTGG 2 cut(s) 13, 57
Bse3DI GCAATG 2 cut(s) 101, 166
BseBI CCWGG 1 cut(s) 88
BseCI ATCGAT 2 cut(s) 435, 773
BseDI CCNNGG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 256, 766
BseMI GCAATG 2 cut(s) 101, 166
BseMII CTCAG 3 cut(s) 288, 574, 676
BseNI ACTGG 2 cut(s) 13, 57
BseRI GAGGAG 1 cut(s) 548
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 1 cut(s) 706
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 3 cut(s) 431, 752, 758
BshNI GGYRCC 1 cut(s) 26
BshVI ATCGAT 2 cut(s) 435, 773
BsiHKAI GWGCWC 2 cut(s) 208, 215
BsiSI CCGG 2 cut(s) 51, 251
BslI CCNNNNNNNGG 2 cut(s) 256, 766
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 3 cut(s) 431, 752, 758
Bsp1286I GDGCHC 2 cut(s) 208, 215
Bsp143I GATC 3 cut(s) 290, 436, 770
BspACI CCGC 5 cut(s) 5, 48, 64, 218, 232
BspANI GGCC 3 cut(s) 431, 752, 758
BspCNI CTCAG 3 cut(s) 289, 573, 675
BspDI ATCGAT 2 cut(s) 435, 773
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 28, 55
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 3 cut(s) 298, 431, 778
BspQI GCTCTTC 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 101, 166
BsrI ACTGG 2 cut(s) 13, 57
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 3 cut(s) 290, 436, 770
BssNI GRCGYC 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 2 cut(s) 210, 670
Bst6I CTCTTC 2 cut(s) 196, 321
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 215, 392
BstDEI CTNAG 3 cut(s) 297, 560, 662
BstDSI CCRYGG 1 cut(s) 753
BstEII GGTNACC 2 cut(s) 7, 356
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 3 cut(s) 293, 439, 773
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 3 cut(s) 290, 436, 770
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 88
BstPI GGTNACC 2 cut(s) 7, 356
BstSCI CCNGG 2 cut(s) 86, 249
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 706
BstV2I GAAGAC 3 cut(s) 244, 594, 693
BstXI CCANNNNNNTGG 1 cut(s) 172
Bsu15I ATCGAT 2 cut(s) 435, 773
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 3 cut(s) 431, 752, 758
BsuTUI ATCGAT 2 cut(s) 435, 773
BtgI CCRYGG 1 cut(s) 753
BtsIMutI CAGTG 4 cut(s) 64, 206, 568, 711
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 2 cut(s) 215, 392
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 757
ClaI ATCGAT 2 cut(s) 435, 773
DdeI CTNAG 3 cut(s) 297, 560, 662
DinI GGCGCC 1 cut(s) 28
DpnI GATC 3 cut(s) 292, 438, 772
DpnII GATC 3 cut(s) 290, 436, 770
Eam1104I CTCTTC 2 cut(s) 196, 321
EarI CTCTTC 2 cut(s) 196, 321
Eco57I CTGAAG 3 cut(s) 483, 606, 687
Eco91I GGTNACC 2 cut(s) 7, 356
EcoO65I GGTNACC 2 cut(s) 7, 356
EcoRII CCWGG 1 cut(s) 86
EgeI GGCGCC 1 cut(s) 28
EheI GGCGCC 1 cut(s) 28
FaiI YATR 5 cut(s) 192, 341, 370, 466, 531
FblI GTMKAC 1 cut(s) 710
Fnu4HI GCNGC 3 cut(s) 218, 232, 695
Fsp4HI GCNGC 3 cut(s) 218, 232, 695
FspBI CTAG 1 cut(s) 221
GlaI GCGC 1 cut(s) 28
GluI GCNGC 3 cut(s) 218, 232, 695
GsuI CTGGAG 1 cut(s) 360
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 3 cut(s) 431, 752, 758
HapII CCGG 2 cut(s) 51, 251
HhaI GCGC 1 cut(s) 29
Hin1I GRCGYC 1 cut(s) 27
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HpaII CCGG 2 cut(s) 51, 251
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 213, 659, 711
Hpy188I TCNGA 5 cut(s) 20, 277, 298, 625, 665
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 3 cut(s) 213, 659, 711
HpyAV CCTTC 3 cut(s) 105, 142, 299
HpyCH4III ACNGT 2 cut(s) 210, 670
HpyCH4V TGCA 4 cut(s) 106, 213, 416, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 3 cut(s) 297, 560, 662
Hsp92I GRCGYC 1 cut(s) 27
HspAI GCGC 1 cut(s) 27
KasI GGCGCC 1 cut(s) 26
Kzo9I GATC 3 cut(s) 290, 436, 770
LguI GCTCTTC 1 cut(s) 196
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 1 cut(s) 706
LweI GCATC 1 cut(s) 390
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 4 cut(s) 7, 59, 223, 356
MalI GATC 3 cut(s) 292, 438, 772
MboI GATC 3 cut(s) 290, 436, 770
MboII GAAGA 5 cut(s) 213, 249, 338, 599, 693
MfeI CAATTG 1 cut(s) 266
MhlI GDGCHC 2 cut(s) 208, 215
MluCI AATT 6 cut(s) 97, 266, 417, 475, 491, 526
Mly113I GGCGCC 1 cut(s) 27
MnlI CCTC 6 cut(s) 292, 316, 322, 564, 569, 742
MseI TTAA 5 cut(s) 44, 96, 525, 546, 597
MspA1I CMGCKG 2 cut(s) 503, 697
MspI CCGG 2 cut(s) 51, 251
MspR9I CCNGG 2 cut(s) 88, 251
MunI CAATTG 1 cut(s) 266
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 400
NarI GGCGCC 1 cut(s) 27
NciI CCSGG 1 cut(s) 251
NdeII GATC 3 cut(s) 290, 436, 770
NlaIV GGNNCC 2 cut(s) 28, 55
NmuCI GTSAC 3 cut(s) 59, 223, 356
PciSI GCTCTTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 219, 233, 696
PluTI GGCGCC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 2 cut(s) 7, 356
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 2 cut(s) 28, 55
PspPI GGNCC 1 cut(s) 757
PvuII CAGCTG 2 cut(s) 503, 697
SapI GCTCTTC 1 cut(s) 196
SaqAI TTAA 5 cut(s) 44, 96, 525, 546, 597
SatI GCNGC 3 cut(s) 218, 232, 695
Sau3AI GATC 3 cut(s) 290, 436, 770
Sau96I GGNCC 1 cut(s) 757
ScrFI CCNGG 2 cut(s) 88, 251
SduI GDGCHC 2 cut(s) 208, 215
SfaNI GCATC 1 cut(s) 390
SfcI CTRYAG 1 cut(s) 711
SfoI GGCGCC 1 cut(s) 28
Sse9I AATT 6 cut(s) 97, 266, 417, 475, 491, 526
SsiI CCGC 5 cut(s) 5, 48, 64, 218, 232
SspDI GGCGCC 1 cut(s) 26
SspI AATATT 1 cut(s) 127
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 86, 249
TaaI ACNGT 2 cut(s) 210, 670
TaqI TCGA 3 cut(s) 79, 435, 773
TasI AATT 6 cut(s) 97, 266, 417, 475, 491, 526
TauI GCSGC 2 cut(s) 220, 234
Tru1I TTAA 5 cut(s) 44, 96, 525, 546, 597
Tru9I TTAA 5 cut(s) 44, 96, 525, 546, 597
TscAI CASTG 4 cut(s) 64, 213, 568, 711
TseFI GTSAC 3 cut(s) 59, 223, 356
TseI GCWGC 1 cut(s) 694
Tsp45I GTSAC 3 cut(s) 59, 223, 356
TspDTI ATGAA 2 cut(s) 90, 530
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 4 cut(s) 64, 213, 568, 711
VneI GTGCAC 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 710
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.