Rroxscaffold_7G00188330

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001524
Physical Location & Seq
Reverse (-)
27901346 .. 27907790
6445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188330.1

Sequence Viewer

Length: 1473 bp
ATGCATGCTCCCCTAACTGCTGATCTTGATGAACCTGAGTTGAGCGACATTCCTGTCCCTGAAACATATTCTCCAAATGTGAAACGCGAGCTGCTGATGCTTTCCTTACCTGCAATTGTGGGTCAGGCAATTGATCCCATAGCAATGCTGATGGAGACTGCTTATATTGGTAGACTGGGCTCTGTGGAGTTGGCCTCAGCTGGTGTATCAATGAACATCTTTAATTATATATCAAAACTATTTAATATCCCTCTCCTCAGTGTTGCTACATCTTTTGTGGCTGAAGACATTGCAAAGTCTGCAAGTAAAGCTTCTACCATAGAAAATGCTTATGAAGACATTACTAATGGAAAATCTGTACCTGCTGAGAGAAAGCAATTATCTTCAGTGTCAACAGCCTTACTTTTAGCACTTGGCATCGGCATGTTTGAGGCGCTAGCCTTGTCTTTGGGATCTGGGTTGTTTCTGAATATGATGGGGATATCATCAAACTCTCCTATGCACATTCCGGCAAAAAGGTTTCTCATAATAAGAGCATTTGGTGCTCCTGCAGTTGTAGCATCTTTGGCTCTTCAAGGAGTTTTCCGTGGTTTTAAGGATACAACAACTCCAGTACTATGTCTAGGTATTGGTAATCTTTTAGCTGCGTTCTTGTTACCAACACTAATATATTATTTTCGTTTGGGTGTAACTGGTGCAGCAATGTCTACTGTTATATCTCAATACACCGTCACATTTTTAATGATCTGGTTTTTAAATAAGAAAGCAGTGCTATTGCCTCCAAAGATGGGATCATTGCAATTTGGTGGATACATAAAATCTGGTGGTTTTCTTCTGGGAAGAACTCTTGCTGTTCTCACGACCATGACATTAGGAACATCAATGGCTGCTCGTCAAGGTCCGGTAGCTATGGCTGCTCATCAGATATGTATACAAGTATGGTTGGCTGTATCCCTTTTAACTGATGCACTCGCTACATCTGGTCAGGCCCTGATTGCGAGTTATTCATCAAAAGGTGAATACAAAATTGTTAAAGAGATTACTGACTTCATGCTGAAGATAGGATTGTTTATAGGTGTTTCATTGGCTGTAATTCTGGGAACATCTTTTGGTTCTATAGCGACCTTGTTCACCAAGGATGCTGCAGTATTGGAGATCCTTAGATCTGGGGTATTGTTTGTCAGTGCTAGTCAACCTTTGACTTCTTTAGCTTATGTTTTCGATGGTCTCCATTATGGTGTTTCAGATTTTGCATATGCAGCTCGTGCCATGATGGTCGTGGGTGCAATATCATCTGTATTTCTGCTATATGCTCCTTCTGTTATCGGTCTTTCTGGGGTTTGGTTGGGTTTGACTCTCTTCATGGGCTTGCGTGCTGTGGCTGGATTTGTCAGATTGCTTTCAAAATCAGGTCCATGGTGGTTCCTGCATAGGGACACTGAGACATCTCAGCTTGTAACCAGCGTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

490

Amino Acids

52.12

Weight (kDa)

8.85

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 129 - 218 3.6e-07 MatE
MatE PF01554 289 - 427 2e-07 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
Acc36I ACCTGC 2 cut(s) 118, 370
AccI GTMKAC 3 cut(s) 172, 707, 931
AccII CGCG 1 cut(s) 87
AclWI GGATC 4 cut(s) 128, 460, 799, 1150
AcuI CTGAAG 3 cut(s) 303, 369, 1076
AfaI GTAC 2 cut(s) 360, 615
AfiI CCNNNNNNNGG 2 cut(s) 788, 1432
AgsI TTSAA 2 cut(s) 575, 1404
AluBI AGCT 8 cut(s) 91, 200, 311, 644, 908, 1211, 1262, 1453
AluI AGCT 8 cut(s) 91, 200, 311, 644, 908, 1211, 1262, 1453
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 3 cut(s) 149, 1232, 1436
AlwI GGATC 4 cut(s) 128, 460, 799, 1150
AlwNI CAGNNNCTG 1 cut(s) 991
AoxI GGCC 2 cut(s) 192, 987
ApeKI GCWGC 7 cut(s) 91, 644, 698, 887, 914, 1142, 1259
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 3 cut(s) 899, 988, 1412
AsuHPI GGTGA 2 cut(s) 1028, 1123
AsuNHI GCTAGC 1 cut(s) 436
AvaII GGWCC 2 cut(s) 899, 1412
BanII GRGCYC 1 cut(s) 182
BauI CACGAG 1 cut(s) 1263
BbsI GAAGAC 2 cut(s) 291, 342
Bbv12I GWGCWC 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 196
BbvI GCAGC 7 cut(s) 78, 631, 710, 874, 901, 1129, 1271
BccI CCATC 5 cut(s) 145, 469, 781, 1217, 1267
BciVI GTATCC 3 cut(s) 592, 803, 961
BcoDI GTCTC 3 cut(s) 149, 1232, 1436
BfaI CTAG 3 cut(s) 437, 623, 1188
BfmI CTRYAG 3 cut(s) 549, 1116, 1143
BfoI RGCGCY 1 cut(s) 437
BfuAI ACCTGC 2 cut(s) 118, 370
BfuI GTATCC 3 cut(s) 592, 803, 961
BglII AGATCT 1 cut(s) 1163
BisI GCNGC 7 cut(s) 92, 645, 699, 888, 915, 1143, 1260
BlsI GCNGC 7 cut(s) 93, 646, 700, 889, 916, 1144, 1261
BmcAI AGTACT 1 cut(s) 615
Bme18I GGWCC 2 cut(s) 899, 1412
BmgT120I GGNCC 3 cut(s) 899, 988, 1412
BmiI GGNNCC 1 cut(s) 1424
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 5 cut(s) 87, 426, 569, 955, 1129
BmtI GCTAGC 1 cut(s) 440
BmuI ACTGGG 1 cut(s) 185
BpiI GAAGAC 2 cut(s) 291, 342
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 1232
BsaJI CCNNGG 3 cut(s) 586, 1134, 1415
BsaWI WCCGGW 1 cut(s) 901
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 592, 622
Bsc4I CCNNNNNNNGG 2 cut(s) 788, 1432
Bse1I ACTGG 3 cut(s) 180, 611, 697
Bse3DI GCAATG 4 cut(s) 150, 288, 708, 794
BseDI CCNNGG 3 cut(s) 586, 1134, 1415
BseGI GGATG 1 cut(s) 1144
BseLI CCNNNNNNNGG 2 cut(s) 788, 1432
BseMI GCAATG 4 cut(s) 150, 288, 708, 794
BseMII CTCAG 6 cut(s) 27, 210, 271, 357, 1431, 1463
BseNI ACTGG 3 cut(s) 180, 611, 697
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 7 cut(s) 78, 631, 710, 874, 901, 1129, 1271
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 194, 989
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 2 cut(s) 509, 902
BslFI GGGAC 2 cut(s) 41, 1448
BslI CCNNNNNNNGG 2 cut(s) 788, 1432
BsmAI GTCTC 3 cut(s) 149, 1232, 1436
BsmFI GGGAC 2 cut(s) 41, 1448
BsnI GGCC 2 cut(s) 194, 989
Bso31I GGTCTC 1 cut(s) 1232
Bsp1286I GDGCHC 2 cut(s) 182, 547
Bsp143I GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
Bsp19I CCATGG 1 cut(s) 1415
BspANI GGCC 2 cut(s) 194, 989
BspCNI CTCAG 6 cut(s) 28, 209, 270, 358, 1432, 1462
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 1424
BspMAI CTGCAG 2 cut(s) 553, 1147
BspMI ACCTGC 2 cut(s) 118, 370
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 4 cut(s) 128, 460, 799, 1150
BspQI GCTCTTC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 1232
BsrDI GCAATG 4 cut(s) 150, 288, 708, 794
BsrI ACTGG 3 cut(s) 180, 611, 697
BssECI CCNNGG 3 cut(s) 586, 1134, 1415
BssMI GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
BssNAI GTATAC 1 cut(s) 932
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 2 cut(s) 1134, 1415
Bst1107I GTATAC 1 cut(s) 932
Bst2BI CACGAG 1 cut(s) 1263
Bst4CI ACNGT 2 cut(s) 712, 730
Bst6I CTCTTC 2 cut(s) 576, 1364
BstAPI GCANNNNNTGC 3 cut(s) 299, 542, 1265
BstC8I GCNNGC 5 cut(s) 6, 89, 438, 1370, 1374
BstDEI CTNAG 7 cut(s) 36, 196, 257, 366, 1160, 1440, 1449
BstDSI CCRYGG 2 cut(s) 586, 1415
BstF5I GGATG 1 cut(s) 1144
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 7 cut(s) 25, 136, 455, 747, 794, 1158, 1166
BstMAI GTCTC 3 cut(s) 149, 1232, 1436
BstMBI GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
BstNSI RCATGY 2 cut(s) 8, 427
BstSFI CTRYAG 3 cut(s) 549, 1116, 1143
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 7 cut(s) 78, 631, 710, 874, 901, 1129, 1271
BstV2I GAAGAC 2 cut(s) 291, 342
BstX2I RGATCY 3 cut(s) 452, 1155, 1163
BstYI RGATCY 3 cut(s) 452, 1155, 1163
BstZ17I GTATAC 1 cut(s) 932
BsuI GTATCC 3 cut(s) 592, 803, 961
BsuRI GGCC 2 cut(s) 194, 989
BtgI CCRYGG 2 cut(s) 586, 1415
BtsCI GGATG 1 cut(s) 1144
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 5 cut(s) 265, 393, 774, 1189, 1437
BveI ACCTGC 2 cut(s) 118, 370
Cac8I GCNNGC 5 cut(s) 6, 89, 438, 1370, 1374
CaiI CAGNNNCTG 1 cut(s) 991
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 899, 988, 1412
Csp6I GTAC 2 cut(s) 359, 614
CviAII CATG 7 cut(s) 5, 424, 865, 1051, 1270, 1363, 1416
CviQI GTAC 2 cut(s) 359, 614
DdeI CTNAG 7 cut(s) 36, 196, 257, 366, 1160, 1440, 1449
DpnI GATC 7 cut(s) 24, 135, 454, 746, 793, 1157, 1165
DpnII GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
DraI TTTAAA 1 cut(s) 756
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 2 cut(s) 576, 1364
EarI CTCTTC 2 cut(s) 576, 1364
Eco130I CCWWGG 2 cut(s) 1134, 1415
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 1232
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 899, 1412
Eco57I CTGAAG 3 cut(s) 303, 369, 1076
EcoO109I RGGNCCY 1 cut(s) 988
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 2 cut(s) 1134, 1415
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 182
ErhI CCWWGG 2 cut(s) 1134, 1415
FaeI CATG 7 cut(s) 8, 427, 868, 1054, 1273, 1366, 1419
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FaqI GGGAC 2 cut(s) 41, 1448
FatI CATG 7 cut(s) 4, 423, 864, 1050, 1269, 1362, 1415
FauNDI CATATG 1 cut(s) 1255
FblI GTMKAC 3 cut(s) 172, 707, 931
Fnu4HI GCNGC 7 cut(s) 92, 645, 699, 888, 915, 1143, 1260
FokI GGATG 1 cut(s) 1151
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 7 cut(s) 92, 645, 699, 888, 915, 1143, 1260
FspBI CTAG 3 cut(s) 437, 623, 1188
GlaI GCGC 1 cut(s) 435
GluI GCNGC 7 cut(s) 92, 645, 699, 888, 915, 1143, 1260
GsuI CTGGAG 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 2 cut(s) 194, 989
HapII CCGG 2 cut(s) 509, 902
HhaI GCGC 1 cut(s) 436
Hin1II CATG 7 cut(s) 8, 427, 868, 1054, 1273, 1366, 1419
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 2 cut(s) 393, 1193
HindII GTYRAC 2 cut(s) 393, 1193
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 1 cut(s) 1354
HpaII CCGG 2 cut(s) 509, 902
HphI GGTGA 2 cut(s) 1028, 1123
Hpy166II GTNNAC 6 cut(s) 173, 393, 708, 932, 1131, 1193
Hpy188I TCNGA 4 cut(s) 468, 924, 1246, 1394
Hpy188III TCNNGA 2 cut(s) 26, 859
Hpy8I GTNNAC 6 cut(s) 173, 393, 708, 932, 1131, 1193
HpyAV CCTTC 1 cut(s) 1326
HpyCH4III ACNGT 2 cut(s) 712, 730
HpyF3I CTNAG 7 cut(s) 36, 196, 257, 366, 1160, 1440, 1449
Hsp92II CATG 7 cut(s) 8, 427, 868, 1054, 1273, 1366, 1419
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 3 cut(s) 13, 550, 1318
Lsp1109I GCAGC 7 cut(s) 78, 631, 710, 874, 901, 1129, 1271
LweI GCATC 5 cut(s) 87, 426, 569, 955, 1129
MaeI CTAG 3 cut(s) 437, 623, 1188
MaeIII GTNAC 4 cut(s) 654, 688, 730, 1456
MalI GATC 7 cut(s) 24, 135, 454, 746, 793, 1157, 1165
MboI GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
MboII GAAGA 8 cut(s) 296, 347, 375, 563, 824, 852, 1069, 1351
MfeI CAATTG 2 cut(s) 114, 129
MflI RGATCY 3 cut(s) 452, 1155, 1163
MhlI GDGCHC 2 cut(s) 182, 547
MluCI AATT 7 cut(s) 114, 129, 223, 377, 800, 1026, 1092
MlyI GAGTC 1 cut(s) 1348
MnlI CCTC 5 cut(s) 205, 261, 266, 424, 789
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 7 cut(s) 222, 243, 594, 740, 755, 959, 1032
MslI CAYNNNNRTG 4 cut(s) 143, 422, 863, 1236
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 2 cut(s) 509, 902
MunI CAATTG 2 cut(s) 114, 129
MvnI CGCG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 1415
NdeI CATATG 1 cut(s) 1255
NdeII GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 7 cut(s) 8, 427, 868, 1054, 1273, 1366, 1419
NlaIV GGNNCC 1 cut(s) 1424
NmuCI GTSAC 1 cut(s) 730
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 427
PaeI GCATGC 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 576
PkrI GCNGC 7 cut(s) 93, 646, 700, 889, 916, 1144, 1261
PleI GAGTC 1 cut(s) 1348
PpsI GAGTC 1 cut(s) 1348
PspN4I GGNNCC 1 cut(s) 1424
PspPI GGNCC 3 cut(s) 899, 988, 1412
PstI CTGCAG 2 cut(s) 553, 1147
PstNI CAGNNNCTG 1 cut(s) 991
PsuI RGATCY 3 cut(s) 452, 1155, 1163
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 2 cut(s) 360, 615
RsaNI GTAC 2 cut(s) 359, 614
RseI CAYNNNNRTG 4 cut(s) 143, 422, 863, 1236
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 7 cut(s) 222, 243, 594, 740, 755, 959, 1032
SatI GCNGC 7 cut(s) 92, 645, 699, 888, 915, 1143, 1260
Sau3AI GATC 7 cut(s) 22, 133, 452, 744, 791, 1155, 1163
Sau96I GGNCC 3 cut(s) 899, 988, 1412
ScaI AGTACT 1 cut(s) 615
SchI GAGTC 1 cut(s) 1348
SduI GDGCHC 2 cut(s) 182, 547
SfaNI GCATC 5 cut(s) 87, 426, 569, 955, 1129
SfcI CTRYAG 3 cut(s) 549, 1116, 1143
SinI GGWCC 2 cut(s) 899, 1412
SmiMI CAYNNNNRTG 4 cut(s) 143, 422, 863, 1236
SphI GCATGC 1 cut(s) 8
Sse9I AATT 7 cut(s) 114, 129, 223, 377, 800, 1026, 1092
SspMI CTAG 3 cut(s) 437, 623, 1188
StyI CCWWGG 2 cut(s) 1134, 1415
TaaI ACNGT 2 cut(s) 712, 730
TaqI TCGA 1 cut(s) 1221
TaqII GACCGA 1 cut(s) 1316
TasI AATT 7 cut(s) 114, 129, 223, 377, 800, 1026, 1092
TatI WGTACW 1 cut(s) 613
Tru1I TTAA 7 cut(s) 222, 243, 594, 740, 755, 959, 1032
Tru9I TTAA 7 cut(s) 222, 243, 594, 740, 755, 959, 1032
TscAI CASTG 5 cut(s) 265, 393, 774, 1189, 1444
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 7 cut(s) 91, 644, 698, 887, 914, 1142, 1259
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 7 cut(s) 45, 227, 348, 996, 1039, 1071, 1351
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 5 cut(s) 265, 393, 774, 1189, 1444
VpaK11BI GGWCC 2 cut(s) 899, 1412
XceI RCATGY 2 cut(s) 8, 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 1276
XmiI GTMKAC 3 cut(s) 172, 707, 931
XspI CTAG 3 cut(s) 437, 623, 1188
ZrmI AGTACT 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.