Rh6DG237200

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001524
Physical Location & Seq
Forward (+)
42209352 .. 42214919
5568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG237200.1

Sequence Viewer

Length: 1794 bp
ATGACGGCTACCCAATTTGGAGGTGGTGCACCTTCTACCGGGTTGAGGAATAGAACCAGTGACTTCAATTCAGTAACTAAGAAAGATTGGGTGTATTCATTGCGGCAAACGGAAGCTAAGTTTGTGGCACTAAGTAATAGAAAGGATTTGAGCAATGTCAGTGCTGTGGGGGGCAGCAATCTGTCATCTAAGCATAGATCACAGTACTCGCGGCTAGTTGCTCATCGCAGAAGGACCCGTTTTCCGGTGGTGGCTAATCAATTGAGTTCGGAGTTTGGTGTCGGTTGCTCTGAGGTTGAGGAAAAGGTGATTCTTAAGGAAATGCATGCTCCCCTAACTGCTGATCGTGATGAACCTGTGTTGAGTGACATTCCTGTCCCTGAAGCATATTCTCCAAATGTGAAACGCGAGCTGCTGATGCTTTCCGTACCTGCAATTGTGGGTCAGGCAATTGATCCCATAGCAATGCTGATGGAGACTGCTTATATTGGTAGACTGGGCTCTGTGGAGTTGGCCTCAGCTGGTGTATCAATGAACATCTTTAATTATATATCAAAACTATTTAATATCCCTCTCCTCAGTGTTGCTACATCTTTTGTGGCTGAAGACATTGCAAAGTCTGCAAGTAAAGCTTCTACTATAGAAAATGCTTATGAAGAAATTACTAATGGAAAATCTGTACCTGCTGAGAGAAAGCAATTATCTTCGGTGTCAACAGCCTTACTTTTAGCACTTGGCATCGGCATGTTTGAGGCGCTAGCCTTGTCTTTGGGATCTGGGTTGTTTCTGAATATGATGGGGATATCATCAAACTCTCCTATGCACATTCCGGCAAAAAGGTTTCTCATAATAAGAGCATTTGGTGCTCCTGCAGTTGTAGCATCTTTGGCTCTTCAAGGAGTTTTCCGTGGTTTTAAGGATACAACAACTCCAGTACTATGTTTAGGTATTGGTAATCTTTTAGCTGCGTTCTTGTTACCAACACTAATATATTATTTTCGTTTGGGTGTAACTGGTGCAGCAATGTCTACTGTTATATCTCAATACACCGTCACATTTTTAATGATCTGGTTTTTAAATAAGAAAGCAGTACTATTGCCTCCAAAGATGGGATCATTGCAATTTGGTGGATACATAAAGTCTGGTGGTTTTCTTCTGGGAAGAACTCTTGCTGTTCTCACGACCATGACATTAGGAACATCAATGGCTGCTCGTCAAGGTCCAGTAGCTATGGCTGCTCATCAGATATGTATACAAGTATGGTTGGCTGTATCCCTTCTAACTGATGCACTTGCTACATCTGGTCAGGCCCTGATTGCGAGTTATTCATCAAAAGGCGAATACAAAATTGTTAAAGAGATTACTGACTTCATGCTGAAGATAGGATTGTTTATAGGTGTTTCATTGGCTGTAATTCTGGGAACATCTTTTGGTTCTATAGCGACCTTGTTCACCAAGGATGCTGCAGTATTGGAGATCCTTAGATCTGGGGTATTGTTTGTCAGTGCTAGTCAACCTTTGACTTCTTTAGCTTATGTTTTCGATGGTCTCCATTATGGTGTTTCAGATTTTGCATATGCAGCTCGTGCCATGATGGTCGTGGGTGCAATATCTTCTGTATTTCTGCTATATGCTCCTTCTGTTATCGGTCTTTCTGGGGTTTGGTTGGGTTTGACTCTCTTCATGGGCTTGCGTGCTGTGGCTGGATTTGTCAGATTGCTTTCAAAATCAGGTCCATGGTGGTTCCTGCATAGGGACACTGAGACATCTCAGCTTGTAACCATCGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE Polysacc_synt_C

Protein Analysis

597

Amino Acids

63.9

Weight (kDa)

9.53

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 236 - 325 4.6e-07 MatE
MatE PF01554 396 - 534 3.6e-07 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
Acc36I ACCTGC 2 cut(s) 439, 691
AccI GTMKAC 3 cut(s) 493, 1028, 1252
AccII CGCG 2 cut(s) 211, 408
AciI CCGC 2 cut(s) 103, 211
AclWI GGATC 4 cut(s) 449, 781, 1120, 1471
AcuI CTGAAG 3 cut(s) 402, 624, 1397
AfaI GTAC 5 cut(s) 206, 429, 681, 936, 1092
AfiI CCNNNNNNNGG 5 cut(s) 38, 45, 244, 1109, 1753
AflII CTTAAG 1 cut(s) 314
AgsI TTSAA 3 cut(s) 67, 896, 1725
AluBI AGCT 9 cut(s) 116, 412, 521, 632, 965, 1229, 1532, 1583, 1774
AluI AGCT 9 cut(s) 116, 412, 521, 632, 965, 1229, 1532, 1583, 1774
Alw21I GWGCWC 2 cut(s) 31, 868
Alw26I GTCTC 3 cut(s) 470, 1553, 1757
Alw44I GTGCAC 1 cut(s) 27
AlwI GGATC 4 cut(s) 449, 781, 1120, 1471
AlwNI CAGNNNCTG 1 cut(s) 1312
AoxI GGCC 2 cut(s) 513, 1308
ApaLI GTGCAC 1 cut(s) 27
ApeKI GCWGC 8 cut(s) 174, 412, 965, 1019, 1208, 1235, 1463, 1580
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 4 cut(s) 234, 1220, 1309, 1733
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 319, 1444
AsuNHI GCTAGC 1 cut(s) 757
AvaII GGWCC 3 cut(s) 234, 1220, 1733
BaeGI GKGCMC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 503
BauI CACGAG 1 cut(s) 1584
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 2 cut(s) 31, 868
BbvCI CCTCAGC 1 cut(s) 517
BbvI GCAGC 8 cut(s) 186, 399, 952, 1031, 1195, 1222, 1450, 1592
BccI CCATC 6 cut(s) 466, 790, 1102, 1538, 1588, 1790
BceAI ACGGC 1 cut(s) 21
BciVI GTATCC 3 cut(s) 913, 1124, 1282
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 3 cut(s) 470, 1553, 1757
BfaI CTAG 3 cut(s) 215, 758, 1509
BfmI CTRYAG 4 cut(s) 639, 870, 1437, 1464
BfoI RGCGCY 1 cut(s) 758
BfrI CTTAAG 1 cut(s) 314
BfuAI ACCTGC 2 cut(s) 439, 691
BfuI GTATCC 3 cut(s) 913, 1124, 1282
BglII AGATCT 1 cut(s) 1484
BmcAI AGTACT 3 cut(s) 206, 936, 1092
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 3 cut(s) 234, 1220, 1733
BmgT120I GGNCC 4 cut(s) 234, 1220, 1309, 1733
BmiI GGNNCC 2 cut(s) 236, 1745
BmrFI CCNGG 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 506
BmsI GCATC 5 cut(s) 408, 747, 890, 1276, 1450
BmtI GCTAGC 1 cut(s) 761
BmuI ACTGGG 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 612
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 1 cut(s) 517
BpuMI CCSGG 1 cut(s) 40
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 3 cut(s) 907, 1455, 1736
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 45, 244, 1109, 1753
Bse1I ACTGG 5 cut(s) 57, 501, 932, 1018, 1223
Bse3DI GCAATG 6 cut(s) 98, 160, 471, 609, 1029, 1115
BseDI CCNNGG 3 cut(s) 907, 1455, 1736
BseGI GGATG 1 cut(s) 1465
BseLI CCNNNNNNNGG 5 cut(s) 38, 45, 244, 1109, 1753
BseMI GCAATG 6 cut(s) 98, 160, 471, 609, 1029, 1115
BseMII CTCAG 6 cut(s) 282, 531, 592, 678, 1752, 1784
BseNI ACTGG 5 cut(s) 57, 501, 932, 1018, 1223
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 8 cut(s) 186, 399, 952, 1031, 1195, 1222, 1450, 1592
BsgI GTGCAG 1 cut(s) 1038
Bsh1236I CGCG 2 cut(s) 211, 408
BshFI GGCC 2 cut(s) 515, 1310
BsiHKAI GWGCWC 2 cut(s) 31, 868
BsiSI CCGG 3 cut(s) 39, 245, 830
BslFI GGGAC 2 cut(s) 362, 1769
BslI CCNNNNNNNGG 5 cut(s) 38, 45, 244, 1109, 1753
BsmAI GTCTC 3 cut(s) 470, 1553, 1757
BsmFI GGGAC 2 cut(s) 362, 1769
BsnI GGCC 2 cut(s) 515, 1310
Bso31I GGTCTC 1 cut(s) 1553
Bsp1286I GDGCHC 3 cut(s) 31, 503, 868
Bsp143I GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
Bsp19I CCATGG 1 cut(s) 1736
BspACI CCGC 2 cut(s) 103, 211
BspANI GGCC 2 cut(s) 515, 1310
BspCNI CTCAG 6 cut(s) 283, 530, 591, 679, 1753, 1783
BspFNI CGCG 2 cut(s) 211, 408
BspLI GGNNCC 2 cut(s) 236, 1745
BspMAI CTGCAG 2 cut(s) 874, 1468
BspMI ACCTGC 2 cut(s) 439, 691
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 4 cut(s) 449, 781, 1120, 1471
BspQI GCTCTTC 1 cut(s) 897
BspTI CTTAAG 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 1553
BsrDI GCAATG 6 cut(s) 98, 160, 471, 609, 1029, 1115
BsrI ACTGG 5 cut(s) 57, 501, 932, 1018, 1223
BssECI CCNNGG 3 cut(s) 907, 1455, 1736
BssMI GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
BssNAI GTATAC 1 cut(s) 1253
BssSI CACGAG 1 cut(s) 1584
BssT1I CCWWGG 2 cut(s) 1455, 1736
Bst1107I GTATAC 1 cut(s) 1253
Bst2BI CACGAG 1 cut(s) 1584
Bst4CI ACNGT 3 cut(s) 204, 1033, 1051
Bst6I CTCTTC 2 cut(s) 897, 1685
BstAFI CTTAAG 1 cut(s) 314
BstAPI GCANNNNNTGC 3 cut(s) 620, 863, 1586
BstC8I GCNNGC 5 cut(s) 327, 410, 759, 1691, 1695
BstDSI CCRYGG 2 cut(s) 907, 1736
BstF5I GGATG 1 cut(s) 1465
BstFNI CGCG 2 cut(s) 211, 408
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 8 cut(s) 200, 346, 457, 776, 1068, 1115, 1479, 1487
BstMAI GTCTC 3 cut(s) 470, 1553, 1757
BstMBI GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
BstNSI RCATGY 2 cut(s) 329, 748
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 4 cut(s) 639, 870, 1437, 1464
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 2 cut(s) 211, 408
BstV1I GCAGC 8 cut(s) 186, 399, 952, 1031, 1195, 1222, 1450, 1592
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 3 cut(s) 773, 1476, 1484
BstYI RGATCY 3 cut(s) 773, 1476, 1484
BstZ17I GTATAC 1 cut(s) 1253
BsuI GTATCC 3 cut(s) 913, 1124, 1282
BsuRI GGCC 2 cut(s) 515, 1310
BtgI CCRYGG 2 cut(s) 907, 1736
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 1465
BtsIMutI CAGTG 5 cut(s) 64, 166, 586, 1510, 1758
BveI ACCTGC 2 cut(s) 439, 691
Cac8I GCNNGC 5 cut(s) 327, 410, 759, 1691, 1695
CaiI CAGNNNCTG 1 cut(s) 1312
CfoI GCGC 1 cut(s) 757
Cfr13I GGNCC 4 cut(s) 234, 1220, 1309, 1733
Csp6I GTAC 5 cut(s) 205, 428, 680, 935, 1091
CviAII CATG 7 cut(s) 326, 745, 1186, 1372, 1591, 1684, 1737
CviQI GTAC 5 cut(s) 205, 428, 680, 935, 1091
DpnI GATC 8 cut(s) 199, 345, 456, 775, 1067, 1114, 1478, 1486
DpnII GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
DraI TTTAAA 1 cut(s) 1077
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 897, 1685
EarI CTCTTC 2 cut(s) 897, 1685
Eco130I CCWWGG 2 cut(s) 1455, 1736
Eco24I GRGCYC 1 cut(s) 503
Eco31I GGTCTC 1 cut(s) 1553
Eco32I GATATC 1 cut(s) 804
Eco47I GGWCC 3 cut(s) 234, 1220, 1733
Eco57I CTGAAG 3 cut(s) 402, 624, 1397
EcoO109I RGGNCCY 2 cut(s) 234, 1309
EcoRV GATATC 1 cut(s) 804
EcoT14I CCWWGG 2 cut(s) 1455, 1736
EcoT22I ATGCAT 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 503
ErhI CCWWGG 2 cut(s) 1455, 1736
FaeI CATG 7 cut(s) 329, 748, 1189, 1375, 1594, 1687, 1740
FalI AAGNNNNNCTT 2 cut(s) 616, 648
FaqI GGGAC 2 cut(s) 362, 1769
FatI CATG 7 cut(s) 325, 744, 1185, 1371, 1590, 1683, 1736
FauNDI CATATG 1 cut(s) 1576
FblI GTMKAC 3 cut(s) 493, 1028, 1252
FokI GGATG 1 cut(s) 1472
FriOI GRGCYC 1 cut(s) 503
FspBI CTAG 3 cut(s) 215, 758, 1509
GlaI GCGC 1 cut(s) 756
GsuI CTGGAG 1 cut(s) 915
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 2 cut(s) 515, 1310
HapII CCGG 3 cut(s) 39, 245, 830
HhaI GCGC 1 cut(s) 757
Hin1II CATG 7 cut(s) 329, 748, 1189, 1375, 1594, 1687, 1740
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HincII GTYRAC 2 cut(s) 714, 1514
HindII GTYRAC 2 cut(s) 714, 1514
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 2 cut(s) 310, 1675
HpaII CCGG 3 cut(s) 39, 245, 830
HphI GGTGA 2 cut(s) 319, 1444
Hpy166II GTNNAC 7 cut(s) 29, 494, 714, 1029, 1253, 1452, 1514
Hpy188I TCNGA 7 cut(s) 271, 292, 789, 1245, 1567, 1715, 1787
Hpy188III TCNNGA 2 cut(s) 347, 1180
Hpy8I GTNNAC 7 cut(s) 29, 494, 714, 1029, 1253, 1452, 1514
HpyAV CCTTC 4 cut(s) 42, 225, 1286, 1647
HpyCH4III ACNGT 3 cut(s) 204, 1033, 1051
Hsp92II CATG 7 cut(s) 329, 748, 1189, 1375, 1594, 1687, 1740
HspAI GCGC 1 cut(s) 755
Kzo9I GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 3 cut(s) 334, 871, 1639
Lsp1109I GCAGC 8 cut(s) 186, 399, 952, 1031, 1195, 1222, 1450, 1592
LweI GCATC 5 cut(s) 408, 747, 890, 1276, 1450
MaeI CTAG 3 cut(s) 215, 758, 1509
MaeIII GTNAC 7 cut(s) 59, 73, 365, 975, 1009, 1051, 1777
MalI GATC 8 cut(s) 199, 345, 456, 775, 1067, 1114, 1478, 1486
MboI GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
MboII GAAGA 9 cut(s) 617, 668, 696, 884, 1145, 1173, 1390, 1605, 1672
MfeI CAATTG 3 cut(s) 260, 435, 450
MflI RGATCY 3 cut(s) 773, 1476, 1484
MhlI GDGCHC 3 cut(s) 31, 503, 868
MlyI GAGTC 1 cut(s) 1669
MnlI CCTC 9 cut(s) 14, 39, 286, 292, 526, 582, 587, 745, 1110
Mph1103I ATGCAT 1 cut(s) 327
MseI TTAA 7 cut(s) 315, 543, 564, 915, 1061, 1076, 1353
MslI CAYNNNNRTG 4 cut(s) 464, 743, 1184, 1557
MspA1I CMGCKG 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 3 cut(s) 39, 245, 830
MspR9I CCNGG 1 cut(s) 40
MunI CAATTG 3 cut(s) 260, 435, 450
MvnI CGCG 2 cut(s) 211, 408
NciI CCSGG 1 cut(s) 40
NcoI CCATGG 1 cut(s) 1736
NdeI CATATG 1 cut(s) 1576
NdeII GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 7 cut(s) 329, 748, 1189, 1375, 1594, 1687, 1740
NlaIV GGNNCC 2 cut(s) 236, 1745
NmuCI GTSAC 3 cut(s) 59, 365, 1051
NsiI ATGCAT 1 cut(s) 327
NspI RCATGY 2 cut(s) 329, 748
PaeI GCATGC 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 897
PfeI GAWTC 1 cut(s) 310
PleI GAGTC 1 cut(s) 1669
PpsI GAGTC 1 cut(s) 1669
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 236, 1745
PspPI GGNCC 4 cut(s) 234, 1220, 1309, 1733
PspPPI RGGWCCY 1 cut(s) 234
PstI CTGCAG 2 cut(s) 874, 1468
PstNI CAGNNNCTG 1 cut(s) 1312
PsuI RGATCY 3 cut(s) 773, 1476, 1484
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 5 cut(s) 206, 429, 681, 936, 1092
RsaNI GTAC 5 cut(s) 205, 428, 680, 935, 1091
RseI CAYNNNNRTG 4 cut(s) 464, 743, 1184, 1557
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 7 cut(s) 315, 543, 564, 915, 1061, 1076, 1353
Sau3AI GATC 8 cut(s) 197, 343, 454, 773, 1065, 1112, 1476, 1484
Sau96I GGNCC 4 cut(s) 234, 1220, 1309, 1733
ScaI AGTACT 3 cut(s) 206, 936, 1092
SchI GAGTC 1 cut(s) 1669
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 3 cut(s) 31, 503, 868
SfaNI GCATC 5 cut(s) 408, 747, 890, 1276, 1450
SfcI CTRYAG 4 cut(s) 639, 870, 1437, 1464
SinI GGWCC 3 cut(s) 234, 1220, 1733
SmiMI CAYNNNNRTG 4 cut(s) 464, 743, 1184, 1557
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SphI GCATGC 1 cut(s) 329
SsiI CCGC 2 cut(s) 103, 211
SspMI CTAG 3 cut(s) 215, 758, 1509
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 2 cut(s) 1455, 1736
TaaI ACNGT 3 cut(s) 204, 1033, 1051
TaqI TCGA 1 cut(s) 1542
TaqII GACCGA 1 cut(s) 1637
TatI WGTACW 3 cut(s) 204, 934, 1090
TauI GCSGC 2 cut(s) 106, 214
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 7 cut(s) 315, 543, 564, 915, 1061, 1076, 1353
Tru9I TTAA 7 cut(s) 315, 543, 564, 915, 1061, 1076, 1353
TscAI CASTG 5 cut(s) 64, 166, 586, 1510, 1765
TseFI GTSAC 3 cut(s) 59, 365, 1051
TseI GCWGC 8 cut(s) 174, 412, 965, 1019, 1208, 1235, 1463, 1580
Tsp45I GTSAC 3 cut(s) 59, 365, 1051
TspDTI ATGAA 8 cut(s) 87, 366, 548, 669, 1317, 1360, 1392, 1672
TspGWI ACGGA 3 cut(s) 125, 415, 896
TspRI CASTG 5 cut(s) 64, 166, 586, 1510, 1765
Vha464I CTTAAG 1 cut(s) 314
VneI GTGCAC 1 cut(s) 27
VpaK11BI GGWCC 3 cut(s) 234, 1220, 1733
XceI RCATGY 2 cut(s) 329, 748
XcmI CCANNNNNNNNNTGG 2 cut(s) 20, 1597
XmiI GTMKAC 3 cut(s) 493, 1028, 1252
XspI CTAG 3 cut(s) 215, 758, 1509
ZrmI AGTACT 3 cut(s) 206, 936, 1092
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.