FvH4_2g16600

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004467
Physical Location & Seq
Forward (+)
14477110 .. 14480457
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16600.t2

Sequence Viewer

Length: 1953 bp
ATGGCTGGGAGGGAGATAGTGATTGAGGGAGTTAGAGGGGAGTGTTATTATGAGAAGAAGAATGTTGTTGTTGGGGGTGGTGAAGATGGAAGCGATGGTAACAGCGTTGAGGAATTGATGGGAGTGATTGAGAGGGTTGGATCGTATTCGGGGTTTCGAAGTGCTCACAGGAAGGAGTGCTTGAGCTTGGTGAGGAGGTTGAAGCTGCTGCTGCCTCTGTTGGAGGAGATTAGGGAGTTGGAGTACTACTCCAATCAGGCTTTGAATTCTTTGGCTAATTTGAAGAGGGCACTTGCTTCTGCCAAGAAGTTGCTCAAGAATTGTAGTTGTGGGAGTAAGATTTACTTGACTTTGGAAAGTGAGGCAGTGATCGTAAGGTTTCATGTTGTTTATGATAAACTAAGCCAGGCTTTGGAAGATTTCCCTTATGTTGAGCTTGGGATCTCAGATGAAGTCAAAGAGCAAGTTCAACTAACGCGAACGCAACTCAAACGAGCAAAGAAACGGGCTGATACGCAGGATATAGAGCTAGCAATGGATCTGATGGTTGTGTTCTCTAAGAAAAATGATGACAGGAATGCAGATAAAGCGATACTTGAAAGACTAGCCAATAAGTTGGAACTACACACTATTGCAGACTTGAAAGAGGAAACAATAGTTGTTAGAAAGCTAGCTAAAAGGAGATCAGCAAGTCAGAATGCTGAAAGCATTCAACAGGTCACTGATCTTTTGGGTAGGTTTAAAGAAAATGCAGGGATTTATGAGGACTTCTTGCTTGATGGTCCTGTTTCCACGAAAAGTCTGAGGAAGTATCGGTCTTTGTTGATTCCAAATGAGTTTCTATGCCCGATTACGTTGGAGATCATGACTGATCCTGTCATTGTTGCAACAGGACAGACTTATGAAAGAGCAAGCATACAAAAGTGGCTGGGTTCCAATCACAACACCTGCCCAAAGACGGGGCAAACATTGGATCACATATCACTAGCACCAAATTTTTCCCTCAAGAATCTAATCCAGCAATGGTGTGAGAAGAATCATTTTGAACTGCCTAAGAAGGAATCTAATGCATTTCCTGATGGCTCTACTGCTGAAATTTTGGAGGAAATATCTTCCTTAGTGTATGATCTATCTTCATGTCAGCTGGATGTTTTGAAAGAAGCCATTTTTAAGATCCGAATGCTATCTAAAGAGAATCCGGAGAATAGAGTTCTGATAGCTAAGGGTGGAGGGATCCCCCAATTGATCAGGCTCTTGTCATATCCAGATTCCAAGATTCAAGAACACACAGTGACAGCTCTTTTAAATTTATCAATTGATGAGGCCAACAAAAGACTCATAGCAGGAGAAGAGTCTGTTTCTGCTATAATTGAGATCTTGCAGCATGGAACAGATGAAGCTAGAGAGAATTCTGCTGCTGCATTGTTGAGCTTATCCATGCTCAATGAGAACAAAGTACTTATTGGGACTTTGAATGGAATTCCTCCTCTGGTACATCTTCTGCAGACTGGAACAACTAGAGGTAGAAAGGATGCTGCCACTGCATTATTTAACTTGTCTTTGAACCAAACCAACAAGTCAAGGGCCATTGATGCAGGTCTAATACCGCCATTGCTTCATCTAATTGAGGATAAGAACTTGGGCATGACTGATGAAGCCCTCTCAATTTTGTTACTCCTTGCATCACATCCTGAGGGCCGAAACGAGATTGGGAGGTTATCTTTCATTGAAACTCTGGTTGGAATTATCAAGAATGGAACTCCCAAGAACAAGGAATGTGCTACATCGGTTCTTCTAGCGCTTGGATTGAATAATTCGTCCTTGACTTTGGCTGCACTTCAGTATGGTGTCTATGAGCATCTGGTGGAGGTAGCTAGATGTGGAACCAACAGAGCTCAAAGGAAGGCTAACGCCCTTTTGCAGCATATGAGTAAATGTGAGCACATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

651

Amino Acids

72.09

Weight (kDa)

6.57

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 98 - 168 2.1e-23 U-box domain
ARM_PUB PF25598 192 - 466 1.6e-24 Plant U-box protein ARM repeat region
Arm PF00514 221 - 260 1.9e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 223 - 423 1.9e-14 ARMC5
Arm PF00514 304 - 342 2.3e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 956
Acc36I ACCTGC 2 cut(s) 956, 1586
AccB7I CCANNNNNTGG 1 cut(s) 412
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 1198
AciI CCGC 1 cut(s) 1607
AclWI GGATC 8 cut(s) 148, 449, 546, 866, 981, 1168, 1228, 1241
AcsI RAATTY 6 cut(s) 265, 994, 1095, 1306, 1408, 1479
AcuI CTGAAG 1 cut(s) 1823
AdeI CACNNNGTG 1 cut(s) 1291
AfaI GTAC 3 cut(s) 245, 1458, 1494
AfeI AGCGCT 1 cut(s) 1800
AfiI CCNNNNNNNGG 3 cut(s) 412, 958, 959
AjnI CCWGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 1722, 1754
Alw21I GWGCWC 3 cut(s) 166, 1897, 1944
AlwI GGATC 8 cut(s) 148, 449, 546, 866, 981, 1168, 1228, 1241
Aor13HI TCCGGA 1 cut(s) 1198
Aor51HI AGCGCT 1 cut(s) 1800
AoxI GGCC 3 cut(s) 1323, 1584, 1696
ApeKI GCWGC 9 cut(s) 205, 208, 211, 1381, 1415, 1418, 1535, 1832, 1921
ApoI RAATTY 6 cut(s) 265, 994, 1095, 1306, 1408, 1479
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 1 cut(s) 1801
AspS9I GGNCC 3 cut(s) 782, 1584, 1696
AsuHPI GGTGA 2 cut(s) 92, 202
AsuII TTCGAA 1 cut(s) 157
AsuNHI GCTAGC 2 cut(s) 529, 670
AvaII GGWCC 1 cut(s) 782
AxyI CCTNAGG 1 cut(s) 1692
BaeGI GKGCMC 1 cut(s) 292
BamHI GGATCC 1 cut(s) 1233
BanII GRGCYC 1 cut(s) 1897
Bbv12I GWGCWC 3 cut(s) 166, 1897, 1944
BbvI GCAGC 9 cut(s) 192, 195, 198, 1393, 1402, 1405, 1522, 1819, 1933
BccI CCATC 6 cut(s) 80, 89, 112, 538, 773, 1073
BciT130I CCWGG 1 cut(s) 407
BclI TGATCA 1 cut(s) 1245
BfaI CTAG 8 cut(s) 530, 605, 671, 986, 1401, 1518, 1796, 1875
BfmI CTRYAG 1 cut(s) 1502
BfoI RGCGCY 1 cut(s) 1802
BfuAI ACCTGC 2 cut(s) 956, 1586
BglII AGATCT 1 cut(s) 1374
BisI GCNGC 9 cut(s) 206, 209, 212, 1382, 1416, 1419, 1536, 1833, 1922
BlsI GCNGC 9 cut(s) 207, 210, 213, 1383, 1417, 1420, 1537, 1834, 1923
BmcAI AGTACT 2 cut(s) 245, 1458
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 3 cut(s) 782, 1584, 1696
BmiI GGNNCC 3 cut(s) 934, 1235, 1885
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 4 cut(s) 1522, 1582, 1691, 1867
BmtI GCTAGC 2 cut(s) 533, 674
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 3 cut(s) 202, 299, 989
BsaWI WCCGGW 1 cut(s) 1198
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 412, 958, 959
Bse1I ACTGG 1 cut(s) 1513
Bse21I CCTNAGG 1 cut(s) 1692
Bse3DI GCAATG 3 cut(s) 540, 1028, 1610
BseAI TCCGGA 1 cut(s) 1198
BseBI CCWGG 1 cut(s) 407
BseGI GGATG 3 cut(s) 1153, 1537, 1687
BseLI CCNNNNNNNGG 3 cut(s) 412, 958, 959
BseMI GCAATG 3 cut(s) 540, 1028, 1610
BseMII CTCAG 3 cut(s) 459, 794, 1683
BseNI ACTGG 1 cut(s) 1513
BseRI GAGGAG 3 cut(s) 208, 239, 1476
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 9 cut(s) 192, 195, 198, 1393, 1402, 1405, 1522, 1819, 1933
BseYI CCCAGC 2 cut(s) 5, 928
BsgI GTGCAG 1 cut(s) 1818
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 3 cut(s) 1325, 1586, 1698
BsiHKAI GWGCWC 3 cut(s) 166, 1897, 1944
BsiSI CCGG 1 cut(s) 1199
BslFI GGGAC 1 cut(s) 1480
BslI CCNNNNNNNGG 3 cut(s) 412, 958, 959
BsmFI GGGAC 1 cut(s) 1480
BsmI GAATGC 4 cut(s) 583, 703, 708, 1185
BsnI GGCC 3 cut(s) 1325, 1586, 1698
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 4 cut(s) 166, 292, 1897, 1944
Bsp13I TCCGGA 1 cut(s) 1198
BspACI CCGC 1 cut(s) 1607
BspANI GGCC 3 cut(s) 1325, 1586, 1698
BspCNI CTCAG 3 cut(s) 458, 795, 1684
BspEI TCCGGA 1 cut(s) 1198
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 1 cut(s) 864
BspLI GGNNCC 3 cut(s) 934, 1235, 1885
BspMAI CTGCAG 1 cut(s) 1506
BspMI ACCTGC 2 cut(s) 956, 1586
BspOI GCTAGC 2 cut(s) 533, 674
BspPI GGATC 8 cut(s) 148, 449, 546, 866, 981, 1168, 1228, 1241
BspT104I TTCGAA 1 cut(s) 157
BsrDI GCAATG 3 cut(s) 540, 1028, 1610
BsrI ACTGG 1 cut(s) 1513
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 1 cut(s) 1291
Bst6I CTCTTC 2 cut(s) 278, 1344
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 531, 672, 913
BstDEI CTNAG 8 cut(s) 401, 445, 558, 803, 1053, 1117, 1221, 1692
BstF5I GGATG 3 cut(s) 1153, 1537, 1687
BstFNI CGCG 1 cut(s) 478
BstH2I RGCGCY 1 cut(s) 1802
BstHHI GCGC 1 cut(s) 1801
BstMWI GCNNNNNNNGC 4 cut(s) 211, 587, 1541, 1592
BstNI CCWGG 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 1502
BstSLI GKGCMC 1 cut(s) 292
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 9 cut(s) 192, 195, 198, 1393, 1402, 1405, 1522, 1819, 1933
BstX2I RGATCY 5 cut(s) 441, 538, 1173, 1233, 1374
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 5 cut(s) 441, 538, 1173, 1233, 1374
Bsu36I CCTNAGG 1 cut(s) 1692
BsuRI GGCC 3 cut(s) 1325, 1586, 1698
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 3 cut(s) 1153, 1537, 1687
BtsI GCAGTG 2 cut(s) 372, 1539
BtsIMutI CAGTG 4 cut(s) 372, 720, 1296, 1539
BveI ACCTGC 2 cut(s) 956, 1586
Cac8I GCNNGC 3 cut(s) 531, 672, 913
CciI TCATGA 1 cut(s) 864
CfoI GCGC 1 cut(s) 1801
Cfr13I GGNCC 3 cut(s) 782, 1584, 1696
Csp6I GTAC 3 cut(s) 244, 1457, 1493
CviAII CATG 6 cut(s) 383, 865, 1137, 1385, 1438, 1645
CviQI GTAC 3 cut(s) 244, 1457, 1493
DdeI CTNAG 8 cut(s) 401, 445, 558, 803, 1053, 1117, 1221, 1692
DraI TTTAAA 2 cut(s) 742, 1305
DraIII CACNNNGTG 1 cut(s) 1291
Eam1104I CTCTTC 2 cut(s) 278, 1344
EarI CTCTTC 2 cut(s) 278, 1344
Ecl136II GAGCTC 1 cut(s) 1895
Eco24I GRGCYC 1 cut(s) 1897
Eco47I GGWCC 1 cut(s) 782
Eco47III AGCGCT 1 cut(s) 1800
Eco53kI GAGCTC 1 cut(s) 1895
Eco57I CTGAAG 1 cut(s) 1823
Eco81I CCTNAGG 1 cut(s) 1692
EcoICRI GAGCTC 1 cut(s) 1895
EcoRI GAATTC 3 cut(s) 265, 1408, 1479
EcoRII CCWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 1897
FaeI CATG 6 cut(s) 386, 868, 1140, 1388, 1441, 1648
FalI AAGNNNNNCTT 8 cut(s) 164, 196, 329, 361, 394, 426, 579, 611
FaqI GGGAC 1 cut(s) 1480
FatI CATG 6 cut(s) 382, 864, 1136, 1384, 1437, 1644
FauNDI CATATG 1 cut(s) 1926
FbaI TGATCA 1 cut(s) 1245
Fnu4HI GCNGC 9 cut(s) 206, 209, 212, 1382, 1416, 1419, 1536, 1833, 1922
FokI GGATG 3 cut(s) 1160, 1544, 1674
FriOI GRGCYC 1 cut(s) 1897
Fsp4HI GCNGC 9 cut(s) 206, 209, 212, 1382, 1416, 1419, 1536, 1833, 1922
FspBI CTAG 8 cut(s) 530, 605, 671, 986, 1401, 1518, 1796, 1875
GlaI GCGC 1 cut(s) 1800
GluI GCNGC 9 cut(s) 206, 209, 212, 1382, 1416, 1419, 1536, 1833, 1922
GsaI CCCAGC 2 cut(s) 9, 932
HaeII RGCGCY 1 cut(s) 1802
HaeIII GGCC 3 cut(s) 1325, 1586, 1698
HapII CCGG 1 cut(s) 1199
HhaI GCGC 1 cut(s) 1801
Hin1II CATG 6 cut(s) 386, 868, 1140, 1388, 1441, 1648
Hin6I GCGC 1 cut(s) 1799
HinP1I GCGC 1 cut(s) 1799
HinfI GANTC 9 cut(s) 826, 1009, 1036, 1061, 1195, 1268, 1276, 1335, 1352
HpaII CCGG 1 cut(s) 1199
HphI GGTGA 2 cut(s) 92, 202
Hpy188I TCNGA 6 cut(s) 448, 543, 696, 804, 1178, 1215
Hpy188III TCNNGA 9 cut(s) 316, 865, 1006, 1076, 1199, 1265, 1280, 1691, 1750
HpyAV CCTTC 3 cut(s) 166, 1051, 1897
HpyCH4III ACNGT 1 cut(s) 1291
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 587, 1541, 1592
HpyF3I CTNAG 8 cut(s) 401, 445, 558, 803, 1053, 1117, 1221, 1692
HpySE526I ACGT 1 cut(s) 854
Hsp92II CATG 6 cut(s) 386, 868, 1140, 1388, 1441, 1648
HspAI GCGC 1 cut(s) 1799
Kpn2I TCCGGA 1 cut(s) 1198
Ksp22I TGATCA 1 cut(s) 1245
Lsp1109I GCAGC 9 cut(s) 192, 195, 198, 1393, 1402, 1405, 1522, 1819, 1933
LweI GCATC 4 cut(s) 1522, 1582, 1691, 1867
MaeI CTAG 8 cut(s) 530, 605, 671, 986, 1401, 1518, 1796, 1875
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 4 cut(s) 98, 718, 1291, 1671
MfeI CAATTG 2 cut(s) 1241, 1314
MflI RGATCY 5 cut(s) 441, 538, 1173, 1233, 1374
MhlI GDGCHC 4 cut(s) 166, 292, 1897, 1944
MlyI GAGTC 2 cut(s) 1329, 1361
MmeI TCCRAC 6 cut(s) 118, 201, 219, 597, 837, 1720
Mph1103I ATGCAT 1 cut(s) 1072
MroI TCCGGA 1 cut(s) 1198
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 5 cut(s) 741, 1170, 1304, 1551, 1951
MspA1I CMGCKG 1 cut(s) 1144
MspI CCGG 1 cut(s) 1199
MspR9I CCNGG 1 cut(s) 407
MunI CAATTG 2 cut(s) 1241, 1314
Mva1269I GAATGC 4 cut(s) 583, 703, 708, 1185
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 4 cut(s) 211, 587, 1541, 1592
NdeI CATATG 1 cut(s) 1926
NheI GCTAGC 2 cut(s) 529, 670
NlaIII CATG 6 cut(s) 386, 868, 1140, 1388, 1441, 1648
NlaIV GGNNCC 3 cut(s) 934, 1235, 1885
NmuCI GTSAC 2 cut(s) 718, 1291
NsiI ATGCAT 1 cut(s) 1072
NspV TTCGAA 1 cut(s) 157
PagI TCATGA 1 cut(s) 864
PaqCI CACCTGC 1 cut(s) 956
PctI GAATGC 4 cut(s) 583, 703, 708, 1185
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 7 cut(s) 826, 1009, 1036, 1061, 1195, 1268, 1276
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 9 cut(s) 207, 210, 213, 1383, 1417, 1420, 1537, 1834, 1923
PleI GAGTC 2 cut(s) 1329, 1360
PpsI GAGTC 2 cut(s) 1329, 1360
Psp124BI GAGCTC 1 cut(s) 1897
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 2 cut(s) 5, 928
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 934, 1235, 1885
PspPI GGNCC 3 cut(s) 782, 1584, 1696
PstI CTGCAG 1 cut(s) 1506
PsuI RGATCY 5 cut(s) 441, 538, 1173, 1233, 1374
PvuII CAGCTG 1 cut(s) 1144
RsaI GTAC 3 cut(s) 245, 1458, 1494
RsaNI GTAC 3 cut(s) 244, 1457, 1493
SacI GAGCTC 1 cut(s) 1897
SaqAI TTAA 5 cut(s) 741, 1170, 1304, 1551, 1951
SatI GCNGC 9 cut(s) 206, 209, 212, 1382, 1416, 1419, 1536, 1833, 1922
Sau96I GGNCC 3 cut(s) 782, 1584, 1696
ScaI AGTACT 2 cut(s) 245, 1458
SchI GAGTC 2 cut(s) 1329, 1361
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 4 cut(s) 166, 292, 1897, 1944
SfaNI GCATC 4 cut(s) 1522, 1582, 1691, 1867
SfcI CTRYAG 1 cut(s) 1502
SfuI TTCGAA 1 cut(s) 157
SinI GGWCC 1 cut(s) 782
SmlI CTYRAG 3 cut(s) 181, 314, 1004
SmoI CTYRAG 3 cut(s) 181, 314, 1004
SsiI CCGC 1 cut(s) 1607
SspMI CTAG 8 cut(s) 530, 605, 671, 986, 1401, 1518, 1796, 1875
SstI GAGCTC 1 cut(s) 1897
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 1291
TaiI ACGT 1 cut(s) 857
TaqI TCGA 1 cut(s) 157
TaqII GACCGA 1 cut(s) 804
TatI WGTACW 2 cut(s) 243, 1456
TfiI GAWTC 7 cut(s) 826, 1009, 1036, 1061, 1195, 1268, 1276
Tru1I TTAA 5 cut(s) 741, 1170, 1304, 1551, 1951
Tru9I TTAA 5 cut(s) 741, 1170, 1304, 1551, 1951
TscAI CASTG 4 cut(s) 372, 727, 1296, 1546
TseFI GTSAC 2 cut(s) 718, 1291
TseI GCWGC 9 cut(s) 205, 208, 211, 1381, 1415, 1418, 1535, 1832, 1921
Tsp45I GTSAC 2 cut(s) 718, 1291
TspDTI ATGAA 8 cut(s) 371, 465, 918, 1125, 1410, 1607, 1668, 1714
TspRI CASTG 4 cut(s) 372, 727, 1296, 1546
Van91I CCANNNNNTGG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 6 cut(s) 265, 994, 1095, 1306, 1408, 1479
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 8 cut(s) 530, 605, 671, 986, 1401, 1518, 1796, 1875
ZrmI AGTACT 2 cut(s) 245, 1458
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.