MD15G1001100.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004467
Physical Location & Seq
Reverse (-)
81995 .. 84759
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1001100.v1.1.491

Sequence Viewer

Length: 1926 bp
ATGGTGATCGATGGAGAGATCAGAGACGAAGGTGAAGGTGGAGGTGGCGGAGGCAGCGGAGGAGGAGGAGAGATCAGAGATGAAACTGCGGAGGGTGTGATTGAATATGTTGCTCAATTCGGAGATTACCGAAGGACGCAGAGGAAAGAATGCTACAACCTGGTGCGGCGTATGAAGCTCTTGCTGCCGCTTTTTCAGGAGACGCGGGAGCTCGATGGGCCAATTCCGGAGAGGGGTGCTGCTTGGTTGGCAAATTTGAAGAAGGTGCTTCTCATGGCAAAGAAATTGTTGAAAACTTGTAATGAGGGGAGCAAAATTTATCTGGCACTTGAAAGCGAGGCAGTCATGGTTAGATTTCATGCGGTTCATGAAAGACTCGGTCAGGCTTTAGAAGGTTTGCCTGATGAACTTTGCCTATCAGATGAAGTTGAGGAACAGGTTGAACTGATGATCATGCAACTTAGACGAGCGAGGAAGCGAACAGATACACAGGACATAGAGCTCGCAATGGATATTATGGTGGTTTTGTCCAAAAAAGAAGATAGAAATGCTGATAGTGCCATTATAGAGAGGCTCGCCCAAAAGCTAGACCTGCAAAAATCCGAGGACCTTAAGATAGAAACAATAGCTGTGAGGAATCTGGTTAAGGATAAAGAGAGAGGAGGGCCAAGTGCAGAGACTACGCAGCAAATCATCGATCTTCTTAACAAATTCAGACAAATCGCAGGAATGGAAGCGACCGATGTACTTGACGGCCCTGTCATGCCTAAAATGCTCGAGAAGTGCCCGTCTTTGATGATCCCTCATGAGTTCCTCTGTCCAATCACACTAGAAATAATGACGGATCCTGTTATTGTTGCAAGTGGACAGACGTATAACAGAGAAAGCATACAAAAGTGGCTCGACACCAATCATAGGACATGTCCCAAGACCAGGCAGACTCTGGCTCACCTGTCACTTGCACCGAATTATGCTCTAAAAAATCTAATCATGCAGTGGTGTGAGAAGAACAAATTCCAGCTCCCGAAAAAAGAAACTTCCACGGGCCAGGAAAGCTCCTCCACCGAACACAAAGAGGAGATCTCATCATTGGTTGACCAACTGTCCTCAAGTCACTTAGAAGTGCAAAGGAATGCGGTCATGAAGATTCGTTTGATCTCTAAAGAGAACCCAGAGAACAGAATTTTGGTTGCCAAAGGTGGAGGAATCCCGCCACTGGTGCAACTTCTTTCCTACCCAGATTCTAAAGTTCAACAGCATGCAGTAACGGCTCTTTTGAACTTATCGATTGATGAAACAAACAAGAAATTGATAACCAGAGAAGAAGCCATTCCAGCTATTATAGAAGTTTTGCAGAATGGAAGCACAGAAGCTAGAGAGAACTCTGCAGCAGCTTTATTTAGCTTGTCGATGATTGATGAGAACAAAGTAATTGTGGGAGAATCAAATGGCATTCCGCCATTAGTAGACTTGTTGCAGGACGGGACTATTAGAGGTAGAAAAGACGCTGCCACGGCACTGTTTAACTTATCTCTGAACCAAGCAAACAAGGGGAGGGCCATTAGTGCTGGCATTGTACCACCACTACTCCAGCTACTCAAGGACAGAAACTTGGGCATGGTCGACGAAGCGCTCTCCATCTTCTTACTTCTTGCATCACATCCAGATGGACGACAAGAGATTGGACAGCTCTCATTCATTGAAATTCTGGTGGAATTTATCCGGGAAGGAACACCCAAGAACAAGGAATGTGCAACGTCAGTTCTTCTCGAGTTGGGGTCAAGCAATTCCTCTTTCTTACTTGCCGCACTGCAGTTTGGAGTGTACGAGCATTTGGTAGAGATCACAAGGAGTGGAACCAACAGAGCTCAAAGGAAGGCAAATGCACTTCTGCAACTCATAAGCAAGAGTGAACAAATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

642

Amino Acids

71.1

Weight (kDa)

5.59

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MLKL_N PF22215 47 - 167 9.6e-06 Mixed lineage kinase domain-like N-terminal domain
PUB10_N PF25368 48 - 157 3.6e-34 PUB10-like, N-terminal domain
U-box PF04564 267 - 338 1.5e-24 U-box domain
ARM_ARMC5 PF24768 357 - 536 4.9e-10 ARMC5
Arm_2 PF04826 359 - 531 7.8e-06 Armadillo-like
ARM_PUB PF25598 360 - 636 2.1e-24 Plant U-box protein ARM repeat region
Arm PF00514 390 - 429 2.1e-09 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 391 - 583 3.1e-14 ARMC5
HEAT_2 PF13646 403 - 506 3.2e-06 HEAT repeats
Arm PF00514 473 - 511 5.8e-08 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 758
Acc36I ACCTGC 1 cut(s) 600
AccI GTMKAC 2 cut(s) 1467, 1623
AccII CGCG 1 cut(s) 205
AccIII TCCGGA 1 cut(s) 226
AclWI GGATC 3 cut(s) 793, 839, 852
AcsI RAATTY 8 cut(s) 253, 315, 710, 1013, 1182, 1704, 1715, 1917
AfaI GTAC 3 cut(s) 747, 1578, 1826
AfeI AGCGCT 1 cut(s) 1632
AfiI CCNNNNNNNGG 4 cut(s) 233, 915, 933, 1216
AflII CTTAAG 1 cut(s) 611
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 8 cut(s) 104, 259, 292, 332, 443, 1253, 1279, 1703
AhdI GACNNNNNGTC 1 cut(s) 1102
AjnI CCWGG 3 cut(s) 159, 932, 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 1169, 1201
Alw21I GWGCWC 3 cut(s) 213, 504, 1870
Alw26I GTCTC 3 cut(s) 18, 194, 671
AlwI GGATC 3 cut(s) 793, 839, 852
AlwNI CAGNNNCTG 1 cut(s) 943
Ama87I CYCGRG 2 cut(s) 776, 1769
Aor13HI TCCGGA 1 cut(s) 226
Aor51HI AGCGCT 1 cut(s) 1632
AoxI GGCC 5 cut(s) 218, 665, 754, 1045, 1557
ApeKI GCWGC 7 cut(s) 54, 184, 239, 685, 1388, 1391, 1508
ApoI RAATTY 8 cut(s) 253, 315, 710, 1013, 1182, 1704, 1715, 1917
Asp700I GAANNNNTTC 3 cut(s) 1013, 1329, 1917
AspLEI GCGC 1 cut(s) 1633
AspS9I GGNCC 6 cut(s) 218, 607, 665, 755, 1045, 1557
AsuC2I CCSGG 1 cut(s) 1724
AsuHPI GGTGA 3 cut(s) 16, 44, 941
AvaI CYCGRG 2 cut(s) 776, 1769
AvaII GGWCC 1 cut(s) 607
BaeGI GKGCMC 1 cut(s) 788
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 3 cut(s) 213, 504, 1870
Bbv12I GWGCWC 3 cut(s) 213, 504, 1870
BbvI GCAGC 7 cut(s) 66, 171, 226, 697, 1400, 1403, 1495
BccI CCATC 4 cut(s) 5, 209, 1646, 1661
BceAI ACGGC 3 cut(s) 769, 1284, 1530
BciT130I CCWGG 3 cut(s) 161, 934, 1049
BclI TGATCA 1 cut(s) 450
BcnI CCSGG 1 cut(s) 1724
BcoDI GTCTC 3 cut(s) 18, 194, 671
BfaI CTAG 3 cut(s) 587, 830, 1374
BfmI CTRYAG 2 cut(s) 1386, 1811
BfoI RGCGCY 1 cut(s) 1634
BfrI CTTAAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 600
BglII AGATCT 1 cut(s) 1080
Bme1390I CCNGG 4 cut(s) 161, 934, 1049, 1724
Bme18I GGWCC 1 cut(s) 607
BmeRI GACNNNNNGTC 1 cut(s) 1102
BmeT110I CYCGRG 2 cut(s) 776, 1769
BmgT120I GGNCC 6 cut(s) 218, 607, 665, 755, 1045, 1557
BmiI GGNNCC 2 cut(s) 846, 1858
BmrFI CCNGG 4 cut(s) 161, 934, 1049, 1724
BmsI GCATC 1 cut(s) 1664
BplI GAGNNNNNCTC 2 cut(s) 1067, 1099
BpmI CTGGAG 1 cut(s) 1574
BpuEI CTTGAG 2 cut(s) 1093, 1583
BpuMI CCSGG 1 cut(s) 1724
Bsa29I ATCGAT 3 cut(s) 9, 696, 1286
BsaJI CCNNGG 3 cut(s) 603, 1041, 1512
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 1572, 1602
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 915, 933, 1216
Bse1I ACTGG 1 cut(s) 1221
Bse3DI GCAATG 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 3 cut(s) 161, 934, 1049
BseCI ATCGAT 3 cut(s) 9, 696, 1286
BseDI CCNNGG 3 cut(s) 603, 1041, 1512
BseGI GGATG 1 cut(s) 1660
BseLI CCNNNNNNNGG 4 cut(s) 233, 915, 933, 1216
BseMI GCAATG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 1221
BseRI GAGGAG 6 cut(s) 75, 78, 81, 675, 1048, 1091
BseSI GKGCMC 1 cut(s) 788
BseXI GCAGC 7 cut(s) 66, 171, 226, 697, 1400, 1403, 1495
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 1 cut(s) 205
Bsh1285I CGRYCG 1 cut(s) 741
BshFI GGCC 5 cut(s) 220, 667, 756, 1047, 1559
BshVI ATCGAT 3 cut(s) 9, 696, 1286
BsiEI CGRYCG 1 cut(s) 741
BsiHKAI GWGCWC 3 cut(s) 213, 504, 1870
BsiHKCI CYCGRG 2 cut(s) 776, 1769
BsiSI CCGG 2 cut(s) 227, 1723
BslFI GGGAC 2 cut(s) 909, 1498
BslI CCNNNNNNNGG 4 cut(s) 233, 915, 933, 1216
BsmAI GTCTC 3 cut(s) 18, 194, 671
BsmBI CGTCTC 2 cut(s) 18, 194
BsmFI GGGAC 2 cut(s) 909, 1498
BsmI GAATGC 3 cut(s) 155, 1138, 1452
BsnI GGCC 5 cut(s) 220, 667, 756, 1047, 1559
BsoBI CYCGRG 2 cut(s) 776, 1769
Bsp1286I GDGCHC 4 cut(s) 213, 504, 788, 1870
Bsp13I TCCGGA 1 cut(s) 226
BspANI GGCC 5 cut(s) 220, 667, 756, 1047, 1559
BspDI ATCGAT 3 cut(s) 9, 696, 1286
BspEI TCCGGA 1 cut(s) 226
BspFNI CGCG 1 cut(s) 205
BspHI TCATGA 3 cut(s) 367, 805, 1140
BspLI GGNNCC 2 cut(s) 846, 1858
BspMAI CTGCAG 2 cut(s) 1390, 1815
BspMI ACCTGC 1 cut(s) 600
BspPI GGATC 3 cut(s) 793, 839, 852
BspTI CTTAAG 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 513
BsrI ACTGG 1 cut(s) 1221
BssECI CCNNGG 3 cut(s) 603, 1041, 1512
Bst2UI CCWGG 3 cut(s) 161, 934, 1049
Bst4CI ACNGT 2 cut(s) 1104, 1521
BstAFI CTTAAG 1 cut(s) 611
BstC8I GCNNGC 4 cut(s) 504, 576, 1260, 1570
BstDEI CTNAG 2 cut(s) 461, 1117
BstDSI CCRYGG 2 cut(s) 1041, 1512
BstF5I GGATG 1 cut(s) 1660
BstFNI CGCG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 1634
BstHHI GCGC 1 cut(s) 1633
BstMAI GTCTC 3 cut(s) 18, 194, 671
BstMCI CGRYCG 1 cut(s) 741
BstNI CCWGG 3 cut(s) 161, 934, 1049
BstNSI RCATGY 2 cut(s) 924, 1262
BstSCI CCNGG 4 cut(s) 159, 932, 1047, 1722
BstSFI CTRYAG 2 cut(s) 1386, 1811
BstSLI GKGCMC 1 cut(s) 788
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 7 cut(s) 66, 171, 226, 697, 1400, 1403, 1495
BstX2I RGATCY 2 cut(s) 844, 1080
BstYI RGATCY 2 cut(s) 844, 1080
Bsu15I ATCGAT 3 cut(s) 9, 696, 1286
BsuRI GGCC 5 cut(s) 220, 667, 756, 1047, 1559
BsuTUI ATCGAT 3 cut(s) 9, 696, 1286
BtgI CCRYGG 2 cut(s) 1041, 1512
BtsCI GGATG 1 cut(s) 1660
BtsI GCAGTG 2 cut(s) 1001, 1808
BtsIMutI CAGTG 4 cut(s) 1001, 1214, 1517, 1808
BveI ACCTGC 1 cut(s) 600
Cac8I GCNNGC 4 cut(s) 504, 576, 1260, 1570
CaiI CAGNNNCTG 1 cut(s) 943
CciI TCATGA 3 cut(s) 367, 805, 1140
CfoI GCGC 1 cut(s) 1633
Cfr13I GGNCC 6 cut(s) 218, 607, 665, 755, 1045, 1557
ClaI ATCGAT 3 cut(s) 9, 696, 1286
CseI GACGC 3 cut(s) 145, 211, 1514
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 3 cut(s) 746, 1577, 1825
CviQI GTAC 3 cut(s) 746, 1577, 1825
DdeI CTNAG 2 cut(s) 461, 1117
DrdI GACNNNNNNGTC 1 cut(s) 758
DriI GACNNNNNGTC 1 cut(s) 1102
DseDI GACNNNNNNGTC 1 cut(s) 758
Eam1105I GACNNNNNGTC 1 cut(s) 1102
EciI GGCGGA 2 cut(s) 63, 1446
Ecl136II GAGCTC 3 cut(s) 211, 502, 1868
Eco24I GRGCYC 3 cut(s) 213, 504, 1870
Eco47I GGWCC 1 cut(s) 607
Eco47III AGCGCT 1 cut(s) 1632
Eco53kI GAGCTC 3 cut(s) 211, 502, 1868
Eco88I CYCGRG 2 cut(s) 776, 1769
EcoICRI GAGCTC 3 cut(s) 211, 502, 1868
EcoO109I RGGNCCY 1 cut(s) 607
EcoRII CCWGG 3 cut(s) 159, 932, 1047
EcoT38I GRGCYC 3 cut(s) 213, 504, 1870
Esp3I CGTCTC 2 cut(s) 18, 194
FaqI GGGAC 2 cut(s) 909, 1498
FauI CCCGC 2 cut(s) 198, 1218
FbaI TGATCA 1 cut(s) 450
FblI GTMKAC 2 cut(s) 1467, 1623
FokI GGATG 1 cut(s) 1647
FriOI GRGCYC 3 cut(s) 213, 504, 1870
FspBI CTAG 3 cut(s) 587, 830, 1374
GlaI GCGC 1 cut(s) 1632
GsuI CTGGAG 1 cut(s) 1574
HaeII RGCGCY 1 cut(s) 1634
HaeIII GGCC 5 cut(s) 220, 667, 756, 1047, 1559
HapII CCGG 2 cut(s) 227, 1723
HgaI GACGC 3 cut(s) 145, 211, 1514
HhaI GCGC 1 cut(s) 1633
Hin6I GCGC 1 cut(s) 1631
HinP1I GCGC 1 cut(s) 1631
HincII GTYRAC 2 cut(s) 1096, 1624
HindII GTYRAC 2 cut(s) 1096, 1624
HinfI GANTC 7 cut(s) 375, 637, 940, 1147, 1206, 1241, 1442
HpaII CCGG 2 cut(s) 227, 1723
HphI GGTGA 3 cut(s) 16, 44, 941
Hpy166II GTNNAC 6 cut(s) 866, 1096, 1468, 1624, 1825, 1913
Hpy188I TCNGA 7 cut(s) 23, 77, 122, 421, 604, 716, 1536
Hpy188III TCNNGA 9 cut(s) 197, 227, 368, 778, 806, 1024, 1141, 1664, 1769
Hpy8I GTNNAC 6 cut(s) 866, 1096, 1468, 1624, 1825, 1913
Hpy99I CGWCG 1 cut(s) 1628
HpyAV CCTTC 7 cut(s) 23, 29, 126, 256, 386, 1721, 1870
HpyCH4III ACNGT 2 cut(s) 1104, 1521
HpyCH4IV ACGT 2 cut(s) 872, 1757
HpyF3I CTNAG 2 cut(s) 461, 1117
HpySE526I ACGT 2 cut(s) 872, 1757
HspAI GCGC 1 cut(s) 1631
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 450
LmnI GCTCC 4 cut(s) 208, 309, 1026, 1061
Lsp1109I GCAGC 7 cut(s) 66, 171, 226, 697, 1400, 1403, 1495
LweI GCATC 1 cut(s) 1664
MabI ACCWGGT 1 cut(s) 159
MaeI CTAG 3 cut(s) 587, 830, 1374
MaeII ACGT 2 cut(s) 872, 1757
MaeIII GTNAC 3 cut(s) 954, 1112, 1264
MboII GAAGA 8 cut(s) 271, 551, 692, 1018, 1156, 1334, 1633, 1757
MflI RGATCY 2 cut(s) 844, 1080
MhlI GDGCHC 4 cut(s) 213, 504, 788, 1870
MlyI GAGTC 2 cut(s) 369, 934
MroI TCCGGA 1 cut(s) 226
MroXI GAANNNNTTC 3 cut(s) 1013, 1329, 1917
MseI TTAA 4 cut(s) 612, 645, 705, 1524
MslI CAYNNNNRTG 1 cut(s) 1665
MspA1I CMGCKG 1 cut(s) 57
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 2 cut(s) 227, 1723
MspR9I CCNGG 4 cut(s) 161, 934, 1049, 1724
Mva1269I GAATGC 3 cut(s) 155, 1138, 1452
MvaI CCWGG 3 cut(s) 161, 934, 1049
MvnI CGCG 1 cut(s) 205
NciI CCSGG 1 cut(s) 1724
NlaIV GGNNCC 2 cut(s) 846, 1858
NmuCI GTSAC 2 cut(s) 954, 1112
NspI RCATGY 2 cut(s) 924, 1262
PaeI GCATGC 1 cut(s) 1262
PaeR7I CTCGAG 2 cut(s) 776, 1769
PagI TCATGA 3 cut(s) 367, 805, 1140
PciI ACATGT 1 cut(s) 920
PctI GAATGC 3 cut(s) 155, 1138, 1452
PdmI GAANNNNTTC 3 cut(s) 1013, 1329, 1917
PfeI GAWTC 5 cut(s) 637, 1147, 1206, 1241, 1442
PflFI GACNNNGTC 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 1722
PleI GAGTC 2 cut(s) 369, 934
PpsI GAGTC 2 cut(s) 369, 934
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 1 cut(s) 920
Psp124BI GAGCTC 3 cut(s) 213, 504, 1870
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 3 cut(s) 159, 932, 1047
PspGI CCWGG 3 cut(s) 159, 932, 1047
PspN4I GGNNCC 2 cut(s) 846, 1858
PspPI GGNCC 6 cut(s) 218, 607, 665, 755, 1045, 1557
PspPPI RGGWCCY 1 cut(s) 607
PstI CTGCAG 2 cut(s) 1390, 1815
PstNI CAGNNNCTG 1 cut(s) 943
PsuI RGATCY 2 cut(s) 844, 1080
PsyI GACNNNGTC 1 cut(s) 378
RsaI GTAC 3 cut(s) 747, 1578, 1826
RsaNI GTAC 3 cut(s) 746, 1577, 1825
RseI CAYNNNNRTG 1 cut(s) 1665
SacI GAGCTC 3 cut(s) 213, 504, 1870
SalI GTCGAC 1 cut(s) 1622
SaqAI TTAA 4 cut(s) 612, 645, 705, 1524
Sau96I GGNCC 6 cut(s) 218, 607, 665, 755, 1045, 1557
SchI GAGTC 2 cut(s) 369, 934
ScrFI CCNGG 4 cut(s) 161, 934, 1049, 1724
SduI GDGCHC 4 cut(s) 213, 504, 788, 1870
SexAI ACCWGGT 1 cut(s) 159
SfaNI GCATC 1 cut(s) 1664
SfcI CTRYAG 2 cut(s) 1386, 1811
Sfr274I CTCGAG 2 cut(s) 776, 1769
SinI GGWCC 1 cut(s) 607
SlaI CTCGAG 2 cut(s) 776, 1769
SmiMI CAYNNNNRTG 1 cut(s) 1665
SmlI CTYRAG 5 cut(s) 611, 776, 1108, 1598, 1769
SmoI CTYRAG 5 cut(s) 611, 776, 1108, 1598, 1769
SphI GCATGC 1 cut(s) 1262
SspMI CTAG 3 cut(s) 587, 830, 1374
SstI GAGCTC 3 cut(s) 213, 504, 1870
StyD4I CCNGG 4 cut(s) 159, 932, 1047, 1722
TaaI ACNGT 2 cut(s) 1104, 1521
TaiI ACGT 2 cut(s) 875, 1760
TaqI TCGA 9 cut(s) 9, 213, 696, 777, 903, 1286, 1409, 1623, 1770
TaqII GACCGA 2 cut(s) 368, 755
TatI WGTACW 1 cut(s) 745
TauI GCSGC 3 cut(s) 169, 190, 1808
TfiI GAWTC 5 cut(s) 637, 1147, 1206, 1241, 1442
Tru1I TTAA 4 cut(s) 612, 645, 705, 1524
Tru9I TTAA 4 cut(s) 612, 645, 705, 1524
TscAI CASTG 4 cut(s) 1001, 1221, 1524, 1815
TseFI GTSAC 2 cut(s) 954, 1112
TseI GCWGC 7 cut(s) 54, 184, 239, 685, 1388, 1391, 1508
Tsp45I GTSAC 2 cut(s) 954, 1112
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 4 cut(s) 1001, 1221, 1524, 1815
Tth111I GACNNNGTC 1 cut(s) 378
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 1 cut(s) 607
XapI RAATTY 8 cut(s) 253, 315, 710, 1013, 1182, 1704, 1715, 1917
XceI RCATGY 2 cut(s) 924, 1262
XcmI CCANNNNNNNNNTGG 1 cut(s) 940
XhoI CTCGAG 2 cut(s) 776, 1769
XmiI GTMKAC 2 cut(s) 1467, 1623
XmnI GAANNNNTTC 3 cut(s) 1013, 1329, 1917
XspI CTAG 3 cut(s) 587, 830, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.