RchiOBHm_Chr6g0280661

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004467
Physical Location & Seq
Forward (+)
44045593 .. 44048835
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25168

Sequence Viewer

Length: 1953 bp
ATGGCAGAGAGGGAGATGGTGATGGAGGGAATAATTAGAAGGGATCATTATCATGGGAAGAAGAATGTTGGTGGTGGTGGTGAAGATGAAAGCGATGGCGGCAGCGTTGAGGAATTGATGGGAGTGATTGAGACCGTTGGATCGTATTCGGGGTTTCGAAGAGCTCACAGGAAGGAGTGCTTGAGCTTGGTGAGAAGGTTGAAGCTATTGCTGCCTCTATTGGAGGAAATAAGGGAGTTGGAGTACTACTCCAATCAGGCTTTGAGCTCTTTGTCTAATTTGAAGAAGGCACTTGCTTCTGCCAAGAAGTTGCTCAAGAATTGTAGCTGTGGGAGTAAGATATACTTGACTCTGGAAAGTGAGGCAATGATGGGAAGGTTTCATGATGTTTATGATAAACTAAGCCAGGCTTTGGATCATTTCCCTTATGTTGAGCTTGGGATCTCAATTGAAGTCAAAGAGCAAGTTCAACTAATGCGAATGCAACTCAAACGAGCAAAGAAACGGGCAGATACACAGGATATAGAGCTGGCAATGGACCTGATGGTTGTGTTCTCTAAGAAAAATGATGACAGGAATGCAGATAAAGCGATACTTGAAAGACTAGCCAACAAGTTGGAACTACACACTATTGCAGACTTGAAAGAGGAAACAATAGCTGTTAGAAAGCTAGCTAAAAGGAGATCAGCAAGGCAGAATGCTGAAAGCATTCAACAGGTCACTGATCTTTTGGGAAGGTTTAAAGAAAATGCAGGGATATATGAGGACTTCTTGCTCGATGGTCCTGTTTCCACGAGAAATCTGAGGCAGTGTCGGTCTTTGTTGATCCCCAATGAGTTTCTATGCCCGATTACGTTGGAGATTATGACTGATCCTGTTATTGTTGCAACTGGACAGACATATGAAAGAGCAAGCATACAAAAGTGGCTGGGTTCCAATCACCACACCTGCCCAAAGACGGGGCAAACATTGGATCATGTATCGCTAGCACCAAATTTTGCTCTCAAGAATCTAATCCAGCAATGGTGTGAGAAGAATCATTTTGAGCTGCCCAAGAAGGATTCTAATGCATTTTCTGATGGCTCTACTGCTGAAATTTTAGAGGAAATATCTTCCTTAGTGTATGATCTATCTTCATGTCAGCTGGATGTTTTAAAAGAAGCCATTTTTAAGATTCGAATGCTCTCTAAAGAGAATCCCGAGAATAGAGTTCTGATAGCTAAGGGTGGAGGGATTCCCCAGTTGATTAAGCTCTTGTCACATCCAGATTCCAAGATTCAAGAACACACAGTGACAGCTCTTCTAAATTTATCGATTGATGAGACCAACAAAAGACTCATAGCAGGGAAAGAGTCTATTCGTGCTATAATTGAGATCTTGCAGCATGGAACAGATGAAGCTAGAGAGAATTCTGCTGCTGCATTGTTCAGCTTATCAATGCTCCATGAGAACAAAGTACTTGTTGGGACTTTGAATGGAATTCCTCCTTTGGTAGATCTTTTGCAGAATGGAACAACCAGAGGTAGAAAGGATGCTGCCACTGCATTATTTAACTTGTCTTTGAACCAAGCCAACAAGTCTAGGGCCATTGACGCAGGTCTAATACCACCCTTGATTCATCTACTTGAGGATAAGAACTTGGGCATGACTGATGAAGCCCTCTCAATTTTGTTACTCCTTGCATCACATCCTGAGGGTCGAAACGAGATTGGGAGATTATCTTTCATTGAAACTCTGGTTGGAATTATCAAGAGTGGAACCCCCAAGAACAAGGAATGTGCTACATCGGTTCTTCTAGAGCTCGGATTGAACAATTCGTCTTTCACTTTGGCTGCACTTCAGTATGGTGTGTATGAGCATCTGGTGGAGGTGGCTAGATGTGGAACCAACAGAGCTCAAAGGAAAGCAAACTCCCTTTTGCAGCATATGAGTAAATGTGAGCACATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

650

Amino Acids

72.43

Weight (kDa)

7.04

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCAfunc PF19584 53 - 159 7.3e-06 MCAfunc domain
MLKL_N PF22215 56 - 176 5.9e-07 Mixed lineage kinase domain-like N-terminal domain
PUB10_N PF25368 57 - 167 9.4e-38 PUB10-like, N-terminal domain
U-box PF04564 276 - 346 1.2e-23 U-box domain
ARM_PUB PF25598 370 - 644 2.3e-24 Plant U-box protein ARM repeat region
Arm PF00514 399 - 438 3e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 401 - 592 3.9e-13 ARMC5
ARM_ARMC5 PF24768 480 - 646 2e-06 ARMC5
Arm PF00514 483 - 520 5.6e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 956
Acc36I ACCTGC 2 cut(s) 956, 1586
AccB7I CCANNNNNTGG 1 cut(s) 412
AciI CCGC 1 cut(s) 99
AclWI GGATC 7 cut(s) 51, 148, 423, 449, 820, 866, 981
AcsI RAATTY 5 cut(s) 994, 1095, 1306, 1408, 1479
AcuI CTGAAG 1 cut(s) 1823
AdeI CACNNNGTG 1 cut(s) 1291
AfaI GTAC 2 cut(s) 245, 1458
AfiI CCNNNNNNNGG 3 cut(s) 412, 958, 959
AjnI CCWGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 1722, 1754
Alw21I GWGCWC 5 cut(s) 166, 269, 1803, 1897, 1944
Alw26I GTCTC 2 cut(s) 125, 1316
AlwI GGATC 7 cut(s) 51, 148, 423, 449, 820, 866, 981
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 1 cut(s) 1584
ApeKI GCWGC 9 cut(s) 102, 211, 1048, 1381, 1415, 1418, 1535, 1832, 1921
ApoI RAATTY 5 cut(s) 994, 1095, 1306, 1408, 1479
Asp700I GAANNNNTTC 1 cut(s) 708
AspS9I GGNCC 3 cut(s) 538, 782, 1584
AsuHPI GGTGA 4 cut(s) 31, 92, 202, 932
AsuII TTCGAA 2 cut(s) 157, 1177
AsuNHI GCTAGC 2 cut(s) 670, 985
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 2 cut(s) 538, 782
AxyI CCTNAGG 1 cut(s) 1692
BanII GRGCYC 4 cut(s) 166, 269, 1803, 1897
BauI CACGAG 1 cut(s) 793
Bbv12I GWGCWC 5 cut(s) 166, 269, 1803, 1897, 1944
BbvI GCAGC 9 cut(s) 114, 198, 1035, 1393, 1402, 1405, 1522, 1819, 1933
BccI CCATC 8 cut(s) 10, 16, 89, 112, 364, 538, 773, 1073
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 125, 1316
BfaI CTAG 7 cut(s) 605, 671, 986, 1401, 1581, 1796, 1875
BfuAI ACCTGC 2 cut(s) 956, 1586
BglII AGATCT 2 cut(s) 1374, 1495
BmcAI AGTACT 2 cut(s) 245, 1458
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 2 cut(s) 538, 782
BmeT110I CYCGRG 1 cut(s) 1199
BmgT120I GGNCC 3 cut(s) 538, 782, 1584
BmiI GGNNCC 3 cut(s) 934, 1759, 1885
BmrFI CCNGG 1 cut(s) 407
BmrI ACTGGG 1 cut(s) 1234
BmsI GCATC 3 cut(s) 1522, 1691, 1867
BmtI GCTAGC 2 cut(s) 674, 989
BmuI ACTGGG 1 cut(s) 1234
BoxI GACNNNNGTC 1 cut(s) 1596
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 2 cut(s) 157, 1177
BpuEI CTTGAG 4 cut(s) 202, 299, 989, 1646
Bsa29I ATCGAT 1 cut(s) 1313
BsaBI GATNNNNATC 1 cut(s) 48
BsaI GGTCTC 2 cut(s) 125, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 412, 958, 959
Bse1I ACTGG 2 cut(s) 895, 1240
Bse21I CCTNAGG 1 cut(s) 1692
Bse3DI GCAATG 3 cut(s) 372, 540, 1028
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 1313
BseGI GGATG 4 cut(s) 1153, 1261, 1537, 1687
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 3 cut(s) 412, 958, 959
BseMI GCAATG 3 cut(s) 372, 540, 1028
BseMII CTCAG 2 cut(s) 794, 1683
BseNI ACTGG 2 cut(s) 895, 1240
BseXI GCAGC 9 cut(s) 114, 198, 1035, 1393, 1402, 1405, 1522, 1819, 1933
BseYI CCCAGC 1 cut(s) 928
BsgI GTGCAG 1 cut(s) 1818
BshFI GGCC 1 cut(s) 1586
BshVI ATCGAT 1 cut(s) 1313
BsiHKAI GWGCWC 5 cut(s) 166, 269, 1803, 1897, 1944
BsiHKCI CYCGRG 1 cut(s) 1199
BslFI GGGAC 1 cut(s) 1480
BslI CCNNNNNNNGG 3 cut(s) 412, 958, 959
BsmAI GTCTC 2 cut(s) 125, 1316
BsmFI GGGAC 1 cut(s) 1480
BsmI GAATGC 5 cut(s) 486, 583, 703, 708, 1185
BsnI GGCC 1 cut(s) 1586
Bso31I GGTCTC 2 cut(s) 125, 1316
BsoBI CYCGRG 1 cut(s) 1199
Bsp119I TTCGAA 2 cut(s) 157, 1177
Bsp1286I GDGCHC 5 cut(s) 166, 269, 1803, 1897, 1944
BspACI CCGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 1586
BspCNI CTCAG 2 cut(s) 795, 1684
BspDI ATCGAT 1 cut(s) 1313
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 934, 1759, 1885
BspMI ACCTGC 2 cut(s) 956, 1586
BspOI GCTAGC 2 cut(s) 674, 989
BspPI GGATC 7 cut(s) 51, 148, 423, 449, 820, 866, 981
BspQI GCTCTTC 2 cut(s) 154, 1305
BspT104I TTCGAA 2 cut(s) 157, 1177
BspTNI GGTCTC 2 cut(s) 125, 1316
BsrDI GCAATG 3 cut(s) 372, 540, 1028
BsrI ACTGG 2 cut(s) 895, 1240
BssSI CACGAG 1 cut(s) 793
Bst2BI CACGAG 1 cut(s) 793
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 2 cut(s) 136, 1291
Bst6I CTCTTC 2 cut(s) 154, 1305
BstBI TTCGAA 2 cut(s) 157, 1177
BstC8I GCNNGC 4 cut(s) 531, 672, 913, 987
BstDEI CTNAG 6 cut(s) 401, 558, 803, 1117, 1221, 1692
BstF5I GGATG 4 cut(s) 1153, 1261, 1537, 1687
BstMAI GTCTC 2 cut(s) 125, 1316
BstMWI GCNNNNNNNGC 5 cut(s) 99, 211, 587, 1541, 1592
BstNI CCWGG 1 cut(s) 407
BstPAI GACNNNNGTC 1 cut(s) 1596
BstSCI CCNGG 1 cut(s) 405
BstV1I GCAGC 9 cut(s) 114, 198, 1035, 1393, 1402, 1405, 1522, 1819, 1933
BstX2I RGATCY 3 cut(s) 441, 1374, 1495
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 3 cut(s) 441, 1374, 1495
Bsu15I ATCGAT 1 cut(s) 1313
Bsu36I CCTNAGG 1 cut(s) 1692
BsuRI GGCC 1 cut(s) 1586
BsuTUI ATCGAT 1 cut(s) 1313
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 4 cut(s) 1153, 1261, 1537, 1687
BtsI GCAGTG 2 cut(s) 815, 1539
BtsIMutI CAGTG 4 cut(s) 720, 815, 1296, 1539
BveI ACCTGC 2 cut(s) 956, 1586
Cac8I GCNNGC 4 cut(s) 531, 672, 913, 987
CciI TCATGA 1 cut(s) 382
Cfr13I GGNCC 3 cut(s) 538, 782, 1584
ClaI ATCGAT 1 cut(s) 1313
CseI GACGC 1 cut(s) 1601
Csp6I GTAC 2 cut(s) 244, 1457
CviAII CATG 7 cut(s) 53, 383, 977, 1137, 1385, 1445, 1645
CviQI GTAC 2 cut(s) 244, 1457
DdeI CTNAG 6 cut(s) 401, 558, 803, 1117, 1221, 1692
DraI TTTAAA 2 cut(s) 742, 1155
DraIII CACNNNGTG 1 cut(s) 1291
Eam1104I CTCTTC 2 cut(s) 154, 1305
EarI CTCTTC 2 cut(s) 154, 1305
Ecl136II GAGCTC 4 cut(s) 164, 267, 1801, 1895
Eco24I GRGCYC 4 cut(s) 166, 269, 1803, 1897
Eco31I GGTCTC 2 cut(s) 125, 1316
Eco47I GGWCC 2 cut(s) 538, 782
Eco53kI GAGCTC 4 cut(s) 164, 267, 1801, 1895
Eco57I CTGAAG 1 cut(s) 1823
Eco81I CCTNAGG 1 cut(s) 1692
Eco88I CYCGRG 1 cut(s) 1199
EcoICRI GAGCTC 4 cut(s) 164, 267, 1801, 1895
EcoRI GAATTC 2 cut(s) 1408, 1479
EcoRII CCWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 4 cut(s) 166, 269, 1803, 1897
FaeI CATG 7 cut(s) 56, 386, 980, 1140, 1388, 1448, 1648
FalI AAGNNNNNCTT 8 cut(s) 164, 196, 329, 361, 394, 426, 579, 611
FaqI GGGAC 1 cut(s) 1480
FatI CATG 7 cut(s) 52, 382, 976, 1136, 1384, 1444, 1644
FauNDI CATATG 2 cut(s) 901, 1926
FokI GGATG 4 cut(s) 1160, 1248, 1544, 1674
FriOI GRGCYC 4 cut(s) 166, 269, 1803, 1897
FspBI CTAG 7 cut(s) 605, 671, 986, 1401, 1581, 1796, 1875
GsaI CCCAGC 1 cut(s) 932
HaeIII GGCC 1 cut(s) 1586
HgaI GACGC 1 cut(s) 1601
Hin1II CATG 7 cut(s) 56, 386, 980, 1140, 1388, 1448, 1648
HphI GGTGA 4 cut(s) 31, 92, 202, 932
Hpy188I TCNGA 4 cut(s) 804, 1078, 1215, 1805
HpyAV CCTTC 7 cut(s) 33, 166, 189, 280, 369, 729, 1051
HpyCH4III ACNGT 2 cut(s) 136, 1291
HpyCH4IV ACGT 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 211, 587, 1541, 1592
HpyF3I CTNAG 6 cut(s) 401, 558, 803, 1117, 1221, 1692
HpySE526I ACGT 1 cut(s) 854
Hsp92II CATG 7 cut(s) 56, 386, 980, 1140, 1388, 1448, 1648
LguI GCTCTTC 2 cut(s) 154, 1305
LmnI GCTCC 1 cut(s) 1446
Lsp1109I GCAGC 9 cut(s) 114, 198, 1035, 1393, 1402, 1405, 1522, 1819, 1933
LweI GCATC 3 cut(s) 1522, 1691, 1867
MaeI CTAG 7 cut(s) 605, 671, 986, 1401, 1581, 1796, 1875
MaeII ACGT 1 cut(s) 854
MaeIII GTNAC 4 cut(s) 718, 1257, 1291, 1671
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 3 cut(s) 441, 1374, 1495
MhlI GDGCHC 5 cut(s) 166, 269, 1803, 1897, 1944
MlyI GAGTC 3 cut(s) 343, 1329, 1361
MmeI TCCRAC 5 cut(s) 118, 219, 597, 837, 1720
Mph1103I ATGCAT 1 cut(s) 1072
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 6 cut(s) 741, 1154, 1170, 1248, 1551, 1951
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 1144
MspR9I CCNGG 1 cut(s) 407
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 5 cut(s) 486, 583, 703, 708, 1185
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 5 cut(s) 99, 211, 587, 1541, 1592
NdeI CATATG 2 cut(s) 901, 1926
NheI GCTAGC 2 cut(s) 670, 985
NlaIII CATG 7 cut(s) 56, 386, 980, 1140, 1388, 1448, 1648
NlaIV GGNNCC 3 cut(s) 934, 1759, 1885
NmuCI GTSAC 3 cut(s) 718, 1257, 1291
NsiI ATGCAT 1 cut(s) 1072
NspV TTCGAA 2 cut(s) 157, 1177
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 956
PciSI GCTCTTC 2 cut(s) 154, 1305
PctI GAATGC 5 cut(s) 486, 583, 703, 708, 1185
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 9 cut(s) 1009, 1036, 1061, 1174, 1195, 1234, 1268, 1276, 1615
PflMI CCANNNNNTGG 1 cut(s) 412
PleI GAGTC 3 cut(s) 343, 1329, 1360
PpsI GAGTC 3 cut(s) 343, 1329, 1360
PshAI GACNNNNGTC 1 cut(s) 1596
Psp124BI GAGCTC 4 cut(s) 166, 269, 1803, 1897
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 934, 1759, 1885
PspPI GGNCC 3 cut(s) 538, 782, 1584
PsuI RGATCY 3 cut(s) 441, 1374, 1495
PvuII CAGCTG 1 cut(s) 1144
RsaI GTAC 2 cut(s) 245, 1458
RsaNI GTAC 2 cut(s) 244, 1457
RseI CAYNNNNRTG 1 cut(s) 51
SacI GAGCTC 4 cut(s) 166, 269, 1803, 1897
SapI GCTCTTC 2 cut(s) 154, 1305
SaqAI TTAA 6 cut(s) 741, 1154, 1170, 1248, 1551, 1951
Sau96I GGNCC 3 cut(s) 538, 782, 1584
ScaI AGTACT 2 cut(s) 245, 1458
SchI GAGTC 3 cut(s) 343, 1329, 1361
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 5 cut(s) 166, 269, 1803, 1897, 1944
SfaNI GCATC 3 cut(s) 1522, 1691, 1867
SfuI TTCGAA 2 cut(s) 157, 1177
SinI GGWCC 2 cut(s) 538, 782
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 4 cut(s) 181, 314, 1004, 1625
SmoI CTYRAG 4 cut(s) 181, 314, 1004, 1625
SsiI CCGC 1 cut(s) 99
SspMI CTAG 7 cut(s) 605, 671, 986, 1401, 1581, 1796, 1875
SstI GAGCTC 4 cut(s) 166, 269, 1803, 1897
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 136, 1291
TaiI ACGT 1 cut(s) 857
TaqI TCGA 5 cut(s) 157, 777, 1177, 1313, 1699
TaqII GACCGA 1 cut(s) 804
TatI WGTACW 2 cut(s) 243, 1456
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 9 cut(s) 1009, 1036, 1061, 1174, 1195, 1234, 1268, 1276, 1615
Tru1I TTAA 6 cut(s) 741, 1154, 1170, 1248, 1551, 1951
Tru9I TTAA 6 cut(s) 741, 1154, 1170, 1248, 1551, 1951
TscAI CASTG 4 cut(s) 727, 815, 1296, 1546
TseFI GTSAC 3 cut(s) 718, 1257, 1291
TseI GCWGC 9 cut(s) 102, 211, 1048, 1381, 1415, 1418, 1535, 1832, 1921
Tsp45I GTSAC 3 cut(s) 718, 1257, 1291
TspDTI ATGAA 8 cut(s) 102, 371, 918, 1125, 1410, 1607, 1668, 1714
TspRI CASTG 4 cut(s) 727, 815, 1296, 1546
Van91I CCANNNNNTGG 1 cut(s) 412
VpaK11BI GGWCC 2 cut(s) 538, 782
XapI RAATTY 5 cut(s) 994, 1095, 1306, 1408, 1479
XbaI TCTAGA 1 cut(s) 1795
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 7 cut(s) 605, 671, 986, 1401, 1581, 1796, 1875
ZrmI AGTACT 2 cut(s) 245, 1458
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.