MD05G1134200.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004467
Physical Location & Seq
Forward (+)
26266061 .. 26268954
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1134200.v1.1.491

Sequence Viewer

Length: 1947 bp
ATGGCAGGGAGGCAGATAATGATGGAAGTTGGGGAGAAGAAGAATGTTGGCAGTTCAACCAAGGGCGAGGATGGTAGCGATGGCGTCGTTGTGGAATTGATGGGAGTGATTGAGACCGTTGGATCGTACTCGGGGTTTCGGAAAACACACAGGAAGGAGTGCGTGAACTTGGTGAGAAGGCTGAAGCTTTTGGTACCACTTCTGGAAGAGATGAAAGAATTGGACATAGTCATGATCATTTCTGCAACAAAGGCTTTGAATTCTCTGGTTAATCTGAAAAATGGACTTTCTTCAGCTAAGAAGTTGCTGAAGAATTGCAGCTCTGGAAGCAAGATTTACTTGACGCTGGAAAGTGAGGCAGTGATGGGAAGATTTCATGCAGTTTATGATAAATTAAGCCAGGCTTTGGATGATATGCCATACAATGAGCTGGGGATCTCAATCGAAGTCAGAGAGCAAGTCGAGCTAATGCGAATGCAGCTCAAACGGGCAAAGAAACGCGCTGACACACAGGACATAGAGCTGGCAATGGACATGATGGTCGTGTTCTCTAAGAAAAAGGAAGGCAGGAATGCAGACAGTGCAATTCTGGAGAGGCTTGCCAATAAGCTGGAACTTCATTCCATTGCAGACTTGGAGGAGGAAACCGTGGCGGTTAGAAAACTTGCTAAGAGGAGATCGGCAAGGCATAATGCTGAAAGCATTCAACAAATCACTGATCTTCTGGGAAAGTTCAAGGAAATTGCAGGGATTTATGAGGATTTCTTGCTCGATGGTCCTGTTTCGACTAGAAGTCTAAGGAGGTGCCAGTCTTTGATGATTCCTCATGAATTCCTCTGTCCAATTACTTTGGAGATCATGACTGATCCTGTCATTGTCGCAAGTGGACAGACTTATGATAGAGCAAGTATACAAAAGTGGCTGGATTCGAATCATCGCACCTGCCCAAAGACAGGACAAAAGTTGGATCACTTATCCCTAGCGCCAAATTTCGCCCTCAACAACCTAATCCGTCAATGGTGTGAGAAGAATAATTATGAACTTCCCAAGAAGGATCCCTGCGCAGGTTCGGATGGCTCTTCTGCTGAAATCATAGAGGAAGTATCTTACTTAGTCCAAAATCTGTCTTCATGTCAGCTGGATGTTTTGAAAGAAGCCATTTATAAAATCCGAATGCTCTCTAAGGAGAATCCGGAGAACAGAATTTTGATTGCTAACAGTGGAGGAATACCGCAGTTGATTAAGCTCCTTTCATATCCGGATTCAAAGATTCAAGAACACACTGTGACAGCGCTTTTAAATTTGTCGATTGATGAGGCGAACAAAAGGCTCATTGCCAGAGAAGGGTCTATTCCTGGTATAATTGAAATCCTGCAGCATGGAACAGATGAAGCTAGAGAGAATTCTGCTGCTGCATTGTTTAGTTTATCGATGCTTGACGAAAACAAAGTACTGGTGGGGGCTTTGAATGGAATTCCTCCCTTGGTAGATCTTCTGCAAAACGGAACAGTCCGTGGAAAAAAGGATGCTGCCACTGCACTATTCAACTTGTCTTTAAACCAAGCCAACAAGTCTAGAGCCATCAACGCCGGCCTAATACCGCCACTGCTTCATTTGCTTGAGGATAAGAACTTGGGCATGACGGATGAAGCCCTCTCGATTTTGTTACTCCTTGCATCACACCCCGAGGGTCGAAATGAAATAGGCCGGCTGTCTTTCATTGAAACCCTGGTCAGGATTATCAAGAACGGGACACCCAAGAACAAGGAATGCGCTGCATCAGTTCTTCTGGTGCTGGGATTGAACAATTCGTCTTTCATTTTGGCTGCACTTCAGTATGGTGTTTATGAGCATCTGGTGGAGCTGGCTAAAAGTGGAACCAACAGAGGTCAAAGGAAAGCAAATTCCCTTTTGCAGCACATGAGTAAATGTGAGCACATACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

649

Amino Acids

71.71

Weight (kDa)

7.93

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 51 - 165 1.7e-38 PUB10-like, N-terminal domain
U-box PF04564 274 - 344 1.2e-23 U-box domain
ARM_PUB PF25598 367 - 642 9.8e-23 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 368 - 544 8e-11 ARMC5
Arm PF00514 397 - 436 1.3e-09 Armadillo/beta-catenin-like repeat
KAP PF05804 399 - 585 8.5e-07 Kinesin-associated protein (KAP)
ARM_ARMC5 PF24768 434 - 595 1.1e-10 ARMC5
Arm PF00514 480 - 518 2.9e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AarI CACCTGC 1 cut(s) 950
AasI GACNNNNNNGTC 1 cut(s) 539
Acc16I TGCGCA 1 cut(s) 1063
Acc36I ACCTGC 2 cut(s) 950, 1055
Acc65I GGTACC 1 cut(s) 193
AccB1I GGYRCC 2 cut(s) 193, 804
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 1 cut(s) 910
AccII CGCG 1 cut(s) 501
AccIII TCCGGA 2 cut(s) 1192, 1258
AciI CCGC 3 cut(s) 653, 1232, 1601
AclWI GGATC 6 cut(s) 130, 443, 860, 975, 1049, 1062
AcsI RAATTY 8 cut(s) 259, 830, 988, 1203, 1300, 1402, 1473, 1903
AcuI CTGAAG 4 cut(s) 203, 276, 329, 1817
AcyI GRCGYC 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 1285
AfaI GTAC 3 cut(s) 128, 195, 1452
AfeI AGCGCT 1 cut(s) 1293
AfiI CCNNNNNNNGG 5 cut(s) 406, 953, 1064, 1344, 1734
AhdI GACNNNNNGTC 1 cut(s) 792
AjnI CCWGG 3 cut(s) 399, 1354, 1728
Alw21I GWGCWC 1 cut(s) 1938
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 6 cut(s) 130, 443, 860, 975, 1049, 1062
Ama87I CYCGRG 2 cut(s) 130, 1685
Aor13HI TCCGGA 2 cut(s) 1192, 1258
Aor51HI AGCGCT 1 cut(s) 1293
AoxI GGCC 2 cut(s) 1591, 1705
ApeKI GCWGC 9 cut(s) 318, 478, 1375, 1409, 1412, 1529, 1775, 1826, 1915
ApoI RAATTY 8 cut(s) 259, 830, 988, 1203, 1300, 1402, 1473, 1903
Asp700I GAANNNNTTC 1 cut(s) 702
Asp718I GGTACC 1 cut(s) 193
AspLEI GCGC 5 cut(s) 503, 985, 1064, 1294, 1775
AspS9I GGNCC 1 cut(s) 776
AsuHPI GGTGA 1 cut(s) 184
AsuII TTCGAA 1 cut(s) 929
AvaI CYCGRG 2 cut(s) 130, 1685
AvaII GGWCC 1 cut(s) 776
BamHI GGATCC 1 cut(s) 1054
BanI GGYRCC 2 cut(s) 193, 804
BbsI GAAGAC 1 cut(s) 1119
Bbv12I GWGCWC 1 cut(s) 1938
BbvI GCAGC 9 cut(s) 330, 490, 1387, 1396, 1399, 1516, 1762, 1813, 1927
BccI CCATC 9 cut(s) 16, 65, 74, 94, 358, 532, 767, 1067, 1589
BciT130I CCWGG 3 cut(s) 401, 1356, 1730
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 4 cut(s) 789, 980, 1395, 1575
BfmI CTRYAG 1 cut(s) 1373
BfoI RGCGCY 2 cut(s) 986, 1295
BfuAI ACCTGC 2 cut(s) 950, 1055
BglII AGATCT 1 cut(s) 1489
BisI GCNGC 9 cut(s) 319, 479, 1376, 1410, 1413, 1530, 1776, 1827, 1916
BlsI GCNGC 9 cut(s) 320, 480, 1377, 1411, 1414, 1531, 1777, 1828, 1917
BmcAI AGTACT 1 cut(s) 1452
Bme1390I CCNGG 3 cut(s) 401, 1356, 1730
Bme18I GGWCC 1 cut(s) 776
BmeRI GACNNNNNGTC 1 cut(s) 792
BmeT110I CYCGRG 2 cut(s) 130, 1685
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 4 cut(s) 195, 806, 1056, 1879
BmrFI CCNGG 3 cut(s) 401, 1356, 1730
BmsI GCATC 5 cut(s) 1422, 1516, 1685, 1787, 1861
BpiI GAAGAC 1 cut(s) 1119
BpmI CTGGAG 1 cut(s) 611
Bpu14I TTCGAA 1 cut(s) 929
BpuEI CTTGAG 1 cut(s) 1640
Bsa29I ATCGAT 1 cut(s) 1430
BsaBI GATNNNNATC 2 cut(s) 440, 930
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 6 cut(s) 60, 648, 1482, 1513, 1686, 1728
BsaWI WCCGGW 2 cut(s) 1192, 1258
Bsc4I CCNNNNNNNGG 5 cut(s) 406, 953, 1064, 1344, 1734
Bse118I RCCGGY 2 cut(s) 1589, 1707
Bse1I ACTGG 2 cut(s) 808, 1458
Bse3DI GCAATG 3 cut(s) 534, 624, 1332
Bse8I GATNNNNATC 2 cut(s) 440, 930
BseAI TCCGGA 2 cut(s) 1192, 1258
BseBI CCWGG 3 cut(s) 401, 1356, 1730
BseCI ATCGAT 1 cut(s) 1430
BseDI CCNNGG 6 cut(s) 60, 648, 1482, 1513, 1686, 1728
BseGI GGATG 6 cut(s) 76, 415, 1078, 1147, 1531, 1651
BseJI GATNNNNATC 2 cut(s) 440, 930
BseLI CCNNNNNNNGG 5 cut(s) 406, 953, 1064, 1344, 1734
BseMI GCAATG 3 cut(s) 534, 624, 1332
BseNI ACTGG 2 cut(s) 808, 1458
BseRI GAGGAG 2 cut(s) 653, 688
BseXI GCAGC 9 cut(s) 330, 490, 1387, 1396, 1399, 1516, 1762, 1813, 1927
BseYI CCCAGC 2 cut(s) 430, 1795
BsgI GTGCAG 2 cut(s) 1521, 1812
Bsh1236I CGCG 1 cut(s) 501
BshFI GGCC 2 cut(s) 1593, 1707
BshNI GGYRCC 2 cut(s) 193, 804
BshVI ATCGAT 1 cut(s) 1430
BsiHKAI GWGCWC 1 cut(s) 1938
BsiHKCI CYCGRG 2 cut(s) 130, 1685
BsiSI CCGG 4 cut(s) 1193, 1259, 1590, 1708
BslFI GGGAC 1 cut(s) 1765
BslI CCNNNNNNNGG 5 cut(s) 406, 953, 1064, 1344, 1734
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 1765
BsmI GAATGC 5 cut(s) 480, 577, 702, 1179, 1775
BsnI GGCC 2 cut(s) 1593, 1707
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 2 cut(s) 130, 1685
Bsp119I TTCGAA 1 cut(s) 929
Bsp1286I GDGCHC 1 cut(s) 1938
Bsp13I TCCGGA 2 cut(s) 1192, 1258
BspACI CCGC 3 cut(s) 653, 1232, 1601
BspANI GGCC 2 cut(s) 1593, 1707
BspDI ATCGAT 1 cut(s) 1430
BspEI TCCGGA 2 cut(s) 1192, 1258
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 3 cut(s) 231, 826, 858
BspLI GGNNCC 4 cut(s) 195, 806, 1056, 1879
BspMAI CTGCAG 1 cut(s) 1377
BspMI ACCTGC 2 cut(s) 950, 1055
BspPI GGATC 6 cut(s) 130, 443, 860, 975, 1049, 1062
BspQI GCTCTTC 1 cut(s) 1084
BspT104I TTCGAA 1 cut(s) 929
BspT107I GGYRCC 2 cut(s) 193, 804
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 3 cut(s) 534, 624, 1332
BsrFI RCCGGY 2 cut(s) 1589, 1707
BsrI ACTGG 2 cut(s) 808, 1458
BssAI RCCGGY 2 cut(s) 1589, 1707
BssECI CCNNGG 6 cut(s) 60, 648, 1482, 1513, 1686, 1728
BssNAI GTATAC 1 cut(s) 911
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 60, 1482
Bst1107I GTATAC 1 cut(s) 911
Bst2UI CCWGG 3 cut(s) 401, 1356, 1730
Bst4CI ACNGT 6 cut(s) 118, 581, 649, 1220, 1285, 1510
Bst6I CTCTTC 2 cut(s) 201, 1084
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 581
BstBI TTCGAA 1 cut(s) 929
BstC8I GCNNGC 5 cut(s) 525, 600, 1591, 1709, 1866
BstDEI CTNAG 6 cut(s) 297, 552, 669, 797, 1111, 1182
BstDSI CCRYGG 2 cut(s) 648, 1513
BstF5I GGATG 6 cut(s) 76, 415, 1078, 1147, 1531, 1651
BstFNI CGCG 1 cut(s) 501
BstH2I RGCGCY 2 cut(s) 986, 1295
BstHHI GCGC 5 cut(s) 503, 985, 1064, 1294, 1775
BstMAI GTCTC 1 cut(s) 107
BstMWI GCNNNNNNNGC 8 cut(s) 251, 327, 463, 478, 581, 1535, 1586, 1615
BstNI CCWGG 3 cut(s) 401, 1356, 1730
BstSCI CCNGG 3 cut(s) 399, 1354, 1728
BstSFI CTRYAG 1 cut(s) 1373
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 9 cut(s) 330, 490, 1387, 1396, 1399, 1516, 1762, 1813, 1927
BstV2I GAAGAC 1 cut(s) 1119
BstX2I RGATCY 3 cut(s) 435, 1054, 1489
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 3 cut(s) 435, 1054, 1489
BstZ17I GTATAC 1 cut(s) 911
Bsu15I ATCGAT 1 cut(s) 1430
BsuRI GGCC 2 cut(s) 1593, 1707
BsuTUI ATCGAT 1 cut(s) 1430
BtgI CCRYGG 2 cut(s) 648, 1513
BtgZI GCGATG 2 cut(s) 93, 920
BtsCI GGATG 6 cut(s) 76, 415, 1078, 1147, 1531, 1651
BtsI GCAGTG 3 cut(s) 366, 1533, 1604
BtsIMutI CAGTG 7 cut(s) 366, 586, 714, 1225, 1281, 1533, 1604
BveI ACCTGC 2 cut(s) 950, 1055
Cac8I GCNNGC 5 cut(s) 525, 600, 1591, 1709, 1866
CciI TCATGA 3 cut(s) 231, 826, 858
CfoI GCGC 5 cut(s) 503, 985, 1064, 1294, 1775
Cfr10I RCCGGY 2 cut(s) 1589, 1707
Cfr13I GGNCC 1 cut(s) 776
ClaI ATCGAT 1 cut(s) 1430
CseI GACGC 2 cut(s) 73, 352
Csp6I GTAC 3 cut(s) 127, 194, 1451
CviAII CATG 9 cut(s) 232, 377, 535, 827, 859, 1131, 1379, 1639, 1921
CviQI GTAC 3 cut(s) 127, 194, 1451
DdeI CTNAG 6 cut(s) 297, 552, 669, 797, 1111, 1182
DraI TTTAAA 2 cut(s) 1299, 1557
DraIII CACNNNGTG 1 cut(s) 1285
DrdI GACNNNNNNGTC 1 cut(s) 539
DriI GACNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 539
Eam1104I CTCTTC 2 cut(s) 201, 1084
Eam1105I GACNNNNNGTC 1 cut(s) 792
EarI CTCTTC 2 cut(s) 201, 1084
Eco130I CCWWGG 2 cut(s) 60, 1482
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 776
Eco47III AGCGCT 1 cut(s) 1293
Eco57I CTGAAG 4 cut(s) 203, 276, 329, 1817
Eco88I CYCGRG 2 cut(s) 130, 1685
EcoRI GAATTC 4 cut(s) 259, 830, 1402, 1473
EcoRII CCWGG 3 cut(s) 399, 1354, 1728
EcoT14I CCWWGG 2 cut(s) 60, 1482
ErhI CCWWGG 2 cut(s) 60, 1482
FaeI CATG 9 cut(s) 235, 380, 538, 830, 862, 1134, 1382, 1642, 1924
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 388, 420
FaqI GGGAC 1 cut(s) 1765
FatI CATG 9 cut(s) 231, 376, 534, 826, 858, 1130, 1378, 1638, 1920
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 9 cut(s) 319, 479, 1376, 1410, 1413, 1530, 1776, 1827, 1916
FokI GGATG 6 cut(s) 83, 422, 1085, 1154, 1538, 1658
Fsp4HI GCNGC 9 cut(s) 319, 479, 1376, 1410, 1413, 1530, 1776, 1827, 1916
FspBI CTAG 4 cut(s) 789, 980, 1395, 1575
FspI TGCGCA 1 cut(s) 1063
GlaI GCGC 5 cut(s) 502, 984, 1063, 1293, 1774
GluI GCNGC 9 cut(s) 319, 479, 1376, 1410, 1413, 1530, 1776, 1827, 1916
GsaI CCCAGC 2 cut(s) 434, 1799
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 2 cut(s) 986, 1295
HaeIII GGCC 2 cut(s) 1593, 1707
HapII CCGG 4 cut(s) 1193, 1259, 1590, 1708
HgaI GACGC 2 cut(s) 73, 352
HhaI GCGC 5 cut(s) 503, 985, 1064, 1294, 1775
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 9 cut(s) 235, 380, 538, 830, 862, 1134, 1382, 1642, 1924
Hin6I GCGC 5 cut(s) 501, 983, 1062, 1292, 1773
HinP1I GCGC 5 cut(s) 501, 983, 1062, 1292, 1773
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 6 cut(s) 820, 926, 931, 1189, 1262, 1270
HpaII CCGG 4 cut(s) 1193, 1259, 1590, 1708
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 3 cut(s) 166, 887, 911
Hpy188I TCNGA 5 cut(s) 141, 276, 452, 1072, 1172
Hpy8I GTNNAC 3 cut(s) 166, 887, 911
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 5 cut(s) 148, 171, 557, 1045, 1337
HpyCH4III ACNGT 6 cut(s) 118, 581, 649, 1220, 1285, 1510
HpyF10VI GCNNNNNNNGC 8 cut(s) 251, 327, 463, 478, 581, 1535, 1586, 1615
HpyF3I CTNAG 6 cut(s) 297, 552, 669, 797, 1111, 1182
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 9 cut(s) 235, 380, 538, 830, 862, 1134, 1382, 1642, 1924
HspAI GCGC 5 cut(s) 501, 983, 1062, 1292, 1773
Kpn2I TCCGGA 2 cut(s) 1192, 1258
KpnI GGTACC 1 cut(s) 197
KroI GCCGGC 2 cut(s) 1589, 1707
KroNI GCCGGC 2 cut(s) 1591, 1709
Ksp22I TGATCA 1 cut(s) 234
LguI GCTCTTC 1 cut(s) 1084
LmnI GCTCC 2 cut(s) 1251, 1861
Lsp1109I GCAGC 9 cut(s) 330, 490, 1387, 1396, 1399, 1516, 1762, 1813, 1927
LweI GCATC 5 cut(s) 1422, 1516, 1685, 1787, 1861
MaeI CTAG 4 cut(s) 789, 980, 1395, 1575
MaeIII GTNAC 2 cut(s) 1285, 1665
MflI RGATCY 3 cut(s) 435, 1054, 1489
MhlI GDGCHC 1 cut(s) 1938
MmeI TCCRAC 2 cut(s) 100, 945
MroI TCCGGA 2 cut(s) 1192, 1258
MroNI GCCGGC 2 cut(s) 1589, 1707
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 5 cut(s) 270, 395, 1242, 1298, 1556
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 1138
MspI CCGG 4 cut(s) 1193, 1259, 1590, 1708
MspR9I CCNGG 3 cut(s) 401, 1356, 1730
Mva1269I GAATGC 5 cut(s) 480, 577, 702, 1179, 1775
MvaI CCWGG 3 cut(s) 401, 1356, 1730
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 8 cut(s) 251, 327, 463, 478, 581, 1535, 1586, 1615
NaeI GCCGGC 2 cut(s) 1591, 1709
NgoMIV GCCGGC 2 cut(s) 1589, 1707
NlaIII CATG 9 cut(s) 235, 380, 538, 830, 862, 1134, 1382, 1642, 1924
NlaIV GGNNCC 4 cut(s) 195, 806, 1056, 1879
NmuCI GTSAC 1 cut(s) 1285
NsbI TGCGCA 1 cut(s) 1063
NspV TTCGAA 1 cut(s) 929
PagI TCATGA 3 cut(s) 231, 826, 858
PaqCI CACCTGC 1 cut(s) 950
PciSI GCTCTTC 1 cut(s) 1084
PctI GAATGC 5 cut(s) 480, 577, 702, 1179, 1775
PdiI GCCGGC 2 cut(s) 1591, 1709
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 6 cut(s) 820, 926, 931, 1189, 1262, 1270
PflFI GACNNNGTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 9 cut(s) 320, 480, 1377, 1411, 1414, 1531, 1777, 1828, 1917
PsiI TTATAA 1 cut(s) 1164
Psp6I CCWGG 3 cut(s) 399, 1354, 1728
PspFI CCCAGC 2 cut(s) 430, 1795
PspGI CCWGG 3 cut(s) 399, 1354, 1728
PspN4I GGNNCC 4 cut(s) 195, 806, 1056, 1879
PspPI GGNCC 1 cut(s) 776
PstI CTGCAG 1 cut(s) 1377
PsuI RGATCY 3 cut(s) 435, 1054, 1489
PsyI GACNNNGTC 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 1138
RsaI GTAC 3 cut(s) 128, 195, 1452
RsaNI GTAC 3 cut(s) 127, 194, 1451
RseI CAYNNNNRTG 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 1084
SaqAI TTAA 5 cut(s) 270, 395, 1242, 1298, 1556
SatI GCNGC 9 cut(s) 319, 479, 1376, 1410, 1413, 1530, 1776, 1827, 1916
Sau96I GGNCC 1 cut(s) 776
ScaI AGTACT 1 cut(s) 1452
ScrFI CCNGG 3 cut(s) 401, 1356, 1730
SduI GDGCHC 1 cut(s) 1938
SfaNI GCATC 5 cut(s) 1422, 1516, 1685, 1787, 1861
SfcI CTRYAG 1 cut(s) 1373
SfuI TTCGAA 1 cut(s) 929
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 1619
SmoI CTYRAG 1 cut(s) 1619
SsiI CCGC 3 cut(s) 653, 1232, 1601
SspMI CTAG 4 cut(s) 789, 980, 1395, 1575
StyD4I CCNGG 3 cut(s) 399, 1354, 1728
StyI CCWWGG 2 cut(s) 60, 1482
TaaI ACNGT 6 cut(s) 118, 581, 649, 1220, 1285, 1510
TaqI TCGA 9 cut(s) 444, 462, 771, 785, 929, 1307, 1430, 1658, 1693
TatI WGTACW 1 cut(s) 1450
TfiI GAWTC 6 cut(s) 820, 926, 931, 1189, 1262, 1270
Tru1I TTAA 5 cut(s) 270, 395, 1242, 1298, 1556
Tru9I TTAA 5 cut(s) 270, 395, 1242, 1298, 1556
TscAI CASTG 7 cut(s) 366, 586, 721, 1225, 1288, 1540, 1611
TseFI GTSAC 1 cut(s) 1285
TseI GCWGC 9 cut(s) 318, 478, 1375, 1409, 1412, 1529, 1775, 1826, 1915
Tsp45I GTSAC 1 cut(s) 1285
TspGWI ACGGA 4 cut(s) 1001, 1502, 1518, 1658
TspRI CASTG 7 cut(s) 366, 586, 721, 1225, 1288, 1540, 1611
Tth111I GACNNNGTC 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 8 cut(s) 259, 830, 988, 1203, 1300, 1402, 1473, 1903
XbaI TCTAGA 1 cut(s) 1574
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 910
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 4 cut(s) 789, 980, 1395, 1575
ZrmI AGTACT 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.