RLG00000013038

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004467
Physical Location & Seq
Reverse (-)
25290166 .. 25292861
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013038

Sequence Viewer

Length: 1974 bp
ATGGCGGAGCGGGAGATGGTGATGGAGGGAATTAGAAGGGATCATTATCGTGGGAAGAAGAATGTTGGTGGTGGTGGTGGTGGTGGTGGTGGTGAAGATGAAAGCGATGGCGGCAGCGTTGAGGAATTGATGGGAGTGATTGAGACCGTTGGATCGTATTCGGGGTTTCGAAGAGCTCACAGGAAGGAGTGCTTGAGCTTGGTGAGGAGGTTGAAGCTATTGCTGCCTCTATTGGAGGAAATAAGGGAGTTGGAGTACTACTCCTCCAACTCCAATCAGGTTTTGAGCTCTTTCTCTAATTTGAAGAAGGCACTTGCTTCTGCCAAGAAGTTGCTCAAGAATTGTAGCTGTGGGAGTAAGATATACTTGACTCTGGAAAGTGAGGCAATGATGGGAAGGTTTCATGCTGTTTATGATAAACTAAGCCAGGCTTTGGATCATTTCCCTTATGTTGAGCTTGGGATCTCAATTGAAGTCAAAGAGCAAGTTCAACTAATGCGAATGCAACTCAAACGAGCAAAGAAACGGGCAGATACACAGGATATAGAGCTAGCAATGGACCTGATGGTTGTGTTCTCTAAGAAAAATGATGACAGGAATGCAGATAAAGCGATACTTGAAAGACTAGCCAACAAGTTGGAACTACATACTATTGCAGACTTGAAAGAGGAAACAATAGCTGTTAGAAAGCTAGCTCAAAGGAGATCAGCAAGGCAGAATGCTGAAAGCATTCAACAGGTCACTGATCTTTTGGGAAGGTTTAAAGAAAATGCAGGGATTTATGAGGACCTCTTGCTCGATGGTCCTGTTTCCACGAGAAATCTGAGGCAGTGTCAGTCTTTGTTGATCCCCAATGAGTTTCTATGCCCGATTACATTGGAGATCATGACTGATCCTGTTATTGTTGCAACTGGACAGACATACGAAAGAGCAAGCATACAGAAGTGGCTGGGTTCCAATCACCACACCTGCCCAAAGACGGGGCAAACATTGGATCATGTATCGCTAGCACCAAATTTTGCTCTCAAGAATCTAATCCAGCAATGGTGTGAGAAGAATCATTTTGAGCTGCCCAAGAAGGATTCTAATGCATTTTCTGATGGCTCTACTGCTGAAATTTTGGAGGAAATATCTTCCTTAGTGTATGATCTATCTTCATGTCAGCTGGATGTTTTAAAAGAAGCCATTTTTAAGATTCGAATGCTCTCTAAAGAGAATCCGGAGAATAGAGTTCTGATAGCTAAGGGCGGAGGAATTCCCCAGTTAATTAAGCTCTTGTCACATCCAGATTCCAAGATTCAAGAACACACAGTGACAGCTCTTCTAAATTTATCGATTGATGAGACCAACAAAAGACTCATAGCAGGAAAAGAGTCTATTCGTGCTATAATTGAGATCTTGCAGCATGGAACAGATGAAGCTAGAGAGAATTCTGCTGCTGCATTGTTCAGCTTATCAATGCTCAATGAGAACAAAGTACTTGTTGGGACTTTGAATGGAATTCCTCCTTTGGTAGATCTTTTGCAGAATGGAACAACCAGAGGTAGAAAGGATGCTGCCACTGCATTGTTTAACTTGTCTTTGAACCAAGCCAACAAGTCTAGGGCCATTGACGCAGGTCTAATACCGCCCTTGATTCATCTACTTGAGGATAAGAACTTGGGCATGACTGATGAAGCCCTCTCAATTTTGTTACTCCTTGCATCACATCCTGAGGGTCGAAACGAGATTGGGAGATTATCTTTCATTGAAACTCTGGTTGGAATTATCAAGAGTGGAACCCCCAAGAACAAGGAATGTGCTACATCGGTTCTTCTAGAGCTTGGATTGAACAATTCGTCTTTCACTTTGGCTGCACTTCAGTATGGTGTGTATGAGCATCTGGTGGAGGTGGCTAGATGTGGAACCAACAGAGCTCAAAGGAAAGCAAACTCCCTTTTGCAGCATATGAGTAAATGTGAGCACATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

658

Amino Acids

72.85

Weight (kDa)

7.0

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MLKL_N PF22215 60 - 183 7.1e-08 Mixed lineage kinase domain-like N-terminal domain
PUB10_N PF25368 61 - 174 6.9e-39 PUB10-like, N-terminal domain
U-box PF04564 283 - 353 1.2e-23 U-box domain
Arm_2 PF04826 376 - 540 5.8e-06 Armadillo-like
ARM_PUB PF25598 377 - 651 1.4e-24 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 391 - 553 6.2e-10 ARMC5
Arm PF00514 406 - 445 3e-08 Armadillo/beta-catenin-like repeat
Arm PF00514 489 - 527 3e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 490 - 653 3.1e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 977
Acc36I ACCTGC 2 cut(s) 977, 1607
AccB7I CCANNNNNTGG 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 10
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 5 cut(s) 5, 10, 111, 1248, 1628
AclWI GGATC 7 cut(s) 48, 160, 444, 470, 841, 887, 1002
AcsI RAATTY 6 cut(s) 1015, 1116, 1254, 1327, 1429, 1500
AcuI CTGAAG 1 cut(s) 1844
AdeI CACNNNGTG 1 cut(s) 1312
AfaI GTAC 2 cut(s) 257, 1479
AfiI CCNNNNNNNGG 3 cut(s) 433, 979, 980
AjnI CCWGG 1 cut(s) 426
AjuI GAANNNNNNNTTGG 2 cut(s) 1743, 1775
Alw21I GWGCWC 4 cut(s) 178, 290, 1918, 1965
Alw26I GTCTC 2 cut(s) 137, 1337
AlwI GGATC 7 cut(s) 48, 160, 444, 470, 841, 887, 1002
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 1 cut(s) 1605
ApeKI GCWGC 9 cut(s) 114, 223, 1069, 1402, 1436, 1439, 1556, 1853, 1942
ApoI RAATTY 6 cut(s) 1015, 1116, 1254, 1327, 1429, 1500
Asp700I GAANNNNTTC 1 cut(s) 729
AspS9I GGNCC 4 cut(s) 559, 787, 803, 1605
AsuHPI GGTGA 4 cut(s) 31, 104, 214, 953
AsuII TTCGAA 2 cut(s) 169, 1198
AsuNHI GCTAGC 3 cut(s) 550, 691, 1006
AvaII GGWCC 3 cut(s) 559, 787, 803
AxyI CCTNAGG 1 cut(s) 1713
BanII GRGCYC 3 cut(s) 178, 290, 1918
BauI CACGAG 1 cut(s) 814
Bbv12I GWGCWC 4 cut(s) 178, 290, 1918, 1965
BbvI GCAGC 9 cut(s) 126, 210, 1056, 1414, 1423, 1426, 1543, 1840, 1954
BccI CCATC 8 cut(s) 10, 16, 101, 124, 385, 559, 794, 1094
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 137, 1337
BfaI CTAG 8 cut(s) 551, 626, 692, 1007, 1422, 1602, 1817, 1896
BfuAI ACCTGC 2 cut(s) 977, 1607
BglII AGATCT 2 cut(s) 1395, 1516
BmcAI AGTACT 2 cut(s) 257, 1479
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 3 cut(s) 559, 787, 803
BmgT120I GGNCC 4 cut(s) 559, 787, 803, 1605
BmiI GGNNCC 3 cut(s) 955, 1780, 1906
BmrFI CCNGG 1 cut(s) 428
BmrI ACTGGG 1 cut(s) 1255
BmsI GCATC 3 cut(s) 1543, 1712, 1888
BmtI GCTAGC 3 cut(s) 554, 695, 1010
BmuI ACTGGG 1 cut(s) 1255
BoxI GACNNNNGTC 1 cut(s) 1617
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu10I CCTNAGC 1 cut(s) 1242
Bpu14I TTCGAA 2 cut(s) 169, 1198
BpuEI CTTGAG 4 cut(s) 214, 320, 1010, 1667
Bsa29I ATCGAT 1 cut(s) 1334
BsaBI GATNNNNATC 1 cut(s) 45
BsaI GGTCTC 2 cut(s) 137, 1337
BsaWI WCCGGW 1 cut(s) 1219
BsaXI ACNNNNNCTCC 4 cut(s) 239, 248, 269, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 979, 980
Bse1I ACTGG 2 cut(s) 916, 1261
Bse21I CCTNAGG 1 cut(s) 1713
Bse3DI GCAATG 3 cut(s) 393, 561, 1049
Bse8I GATNNNNATC 1 cut(s) 45
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 428
BseCI ATCGAT 1 cut(s) 1334
BseGI GGATG 4 cut(s) 1174, 1282, 1558, 1708
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 3 cut(s) 433, 979, 980
BseMI GCAATG 3 cut(s) 393, 561, 1049
BseMII CTCAG 2 cut(s) 815, 1704
BseNI ACTGG 2 cut(s) 916, 1261
BseRI GAGGAG 2 cut(s) 220, 253
BseXI GCAGC 9 cut(s) 126, 210, 1056, 1414, 1423, 1426, 1543, 1840, 1954
BseYI CCCAGC 1 cut(s) 949
BsgI GTGCAG 1 cut(s) 1839
BshFI GGCC 1 cut(s) 1607
BshVI ATCGAT 1 cut(s) 1334
BsiHKAI GWGCWC 4 cut(s) 178, 290, 1918, 1965
BsiSI CCGG 1 cut(s) 1220
BslFI GGGAC 1 cut(s) 1501
BslI CCNNNNNNNGG 3 cut(s) 433, 979, 980
BsmAI GTCTC 2 cut(s) 137, 1337
BsmFI GGGAC 1 cut(s) 1501
BsmI GAATGC 5 cut(s) 507, 604, 724, 729, 1206
BsnI GGCC 1 cut(s) 1607
Bso31I GGTCTC 2 cut(s) 137, 1337
Bsp119I TTCGAA 2 cut(s) 169, 1198
Bsp1286I GDGCHC 4 cut(s) 178, 290, 1918, 1965
Bsp13I TCCGGA 1 cut(s) 1219
BspACI CCGC 5 cut(s) 5, 10, 111, 1248, 1628
BspANI GGCC 1 cut(s) 1607
BspCNI CTCAG 2 cut(s) 816, 1705
BspDI ATCGAT 1 cut(s) 1334
BspEI TCCGGA 1 cut(s) 1219
BspHI TCATGA 1 cut(s) 885
BspLI GGNNCC 3 cut(s) 955, 1780, 1906
BspMI ACCTGC 2 cut(s) 977, 1607
BspOI GCTAGC 3 cut(s) 554, 695, 1010
BspPI GGATC 7 cut(s) 48, 160, 444, 470, 841, 887, 1002
BspQI GCTCTTC 2 cut(s) 166, 1326
BspT104I TTCGAA 2 cut(s) 169, 1198
BspTNI GGTCTC 2 cut(s) 137, 1337
BsrBI CCGCTC 1 cut(s) 10
BsrDI GCAATG 3 cut(s) 393, 561, 1049
BsrI ACTGG 2 cut(s) 916, 1261
BssSI CACGAG 1 cut(s) 814
Bst2BI CACGAG 1 cut(s) 814
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 2 cut(s) 148, 1312
Bst6I CTCTTC 2 cut(s) 166, 1326
BstBI TTCGAA 2 cut(s) 169, 1198
BstC8I GCNNGC 4 cut(s) 552, 693, 934, 1008
BstDEI CTNAG 6 cut(s) 422, 579, 824, 1138, 1242, 1713
BstF5I GGATG 4 cut(s) 1174, 1282, 1558, 1708
BstMAI GTCTC 2 cut(s) 137, 1337
BstMWI GCNNNNNNNGC 5 cut(s) 111, 223, 608, 1562, 1613
BstNI CCWGG 1 cut(s) 428
BstPAI GACNNNNGTC 1 cut(s) 1617
BstSCI CCNGG 1 cut(s) 426
BstV1I GCAGC 9 cut(s) 126, 210, 1056, 1414, 1423, 1426, 1543, 1840, 1954
BstX2I RGATCY 3 cut(s) 462, 1395, 1516
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 3 cut(s) 462, 1395, 1516
Bsu15I ATCGAT 1 cut(s) 1334
Bsu36I CCTNAGG 1 cut(s) 1713
BsuRI GGCC 1 cut(s) 1607
BsuTUI ATCGAT 1 cut(s) 1334
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 4 cut(s) 1174, 1282, 1558, 1708
BtsI GCAGTG 2 cut(s) 836, 1560
BtsIMutI CAGTG 4 cut(s) 741, 836, 1317, 1560
BveI ACCTGC 2 cut(s) 977, 1607
Cac8I GCNNGC 4 cut(s) 552, 693, 934, 1008
CciI TCATGA 1 cut(s) 885
Cfr13I GGNCC 4 cut(s) 559, 787, 803, 1605
ClaI ATCGAT 1 cut(s) 1334
CseI GACGC 1 cut(s) 1622
Csp6I GTAC 2 cut(s) 256, 1478
CviAII CATG 6 cut(s) 404, 886, 998, 1158, 1406, 1666
CviQI GTAC 2 cut(s) 256, 1478
DdeI CTNAG 6 cut(s) 422, 579, 824, 1138, 1242, 1713
DraI TTTAAA 2 cut(s) 763, 1176
DraIII CACNNNGTG 1 cut(s) 1312
Eam1104I CTCTTC 2 cut(s) 166, 1326
EarI CTCTTC 2 cut(s) 166, 1326
EciI GGCGGA 2 cut(s) 20, 1263
Ecl136II GAGCTC 3 cut(s) 176, 288, 1916
Eco24I GRGCYC 3 cut(s) 178, 290, 1918
Eco31I GGTCTC 2 cut(s) 137, 1337
Eco47I GGWCC 3 cut(s) 559, 787, 803
Eco53kI GAGCTC 3 cut(s) 176, 288, 1916
Eco57I CTGAAG 1 cut(s) 1844
Eco81I CCTNAGG 1 cut(s) 1713
EcoICRI GAGCTC 3 cut(s) 176, 288, 1916
EcoO109I RGGNCCY 1 cut(s) 787
EcoRI GAATTC 3 cut(s) 1254, 1429, 1500
EcoRII CCWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 1093
EcoT38I GRGCYC 3 cut(s) 178, 290, 1918
FaeI CATG 6 cut(s) 407, 889, 1001, 1161, 1409, 1669
FalI AAGNNNNNCTT 8 cut(s) 176, 208, 350, 382, 415, 447, 600, 632
FaqI GGGAC 1 cut(s) 1501
FatI CATG 6 cut(s) 403, 885, 997, 1157, 1405, 1665
FauI CCCGC 1 cut(s) 3
FauNDI CATATG 1 cut(s) 1947
FokI GGATG 4 cut(s) 1181, 1269, 1565, 1695
FriOI GRGCYC 3 cut(s) 178, 290, 1918
FspBI CTAG 8 cut(s) 551, 626, 692, 1007, 1422, 1602, 1817, 1896
GsaI CCCAGC 1 cut(s) 953
HaeIII GGCC 1 cut(s) 1607
HapII CCGG 1 cut(s) 1220
HgaI GACGC 1 cut(s) 1622
Hin1II CATG 6 cut(s) 407, 889, 1001, 1161, 1409, 1669
HpaII CCGG 1 cut(s) 1220
HphI GGTGA 4 cut(s) 31, 104, 214, 953
Hpy188I TCNGA 3 cut(s) 825, 1099, 1236
HpyAV CCTTC 6 cut(s) 30, 178, 301, 390, 750, 1072
HpyCH4III ACNGT 2 cut(s) 148, 1312
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 223, 608, 1562, 1613
HpyF3I CTNAG 6 cut(s) 422, 579, 824, 1138, 1242, 1713
Hsp92II CATG 6 cut(s) 407, 889, 1001, 1161, 1409, 1669
Kpn2I TCCGGA 1 cut(s) 1219
LguI GCTCTTC 2 cut(s) 166, 1326
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 9 cut(s) 126, 210, 1056, 1414, 1423, 1426, 1543, 1840, 1954
LweI GCATC 3 cut(s) 1543, 1712, 1888
MaeI CTAG 8 cut(s) 551, 626, 692, 1007, 1422, 1602, 1817, 1896
MaeIII GTNAC 4 cut(s) 739, 1278, 1312, 1692
MbiI CCGCTC 1 cut(s) 10
MfeI CAATTG 1 cut(s) 468
MflI RGATCY 3 cut(s) 462, 1395, 1516
MhlI GDGCHC 4 cut(s) 178, 290, 1918, 1965
MlyI GAGTC 3 cut(s) 364, 1350, 1382
MmeI TCCRAC 5 cut(s) 130, 231, 291, 618, 1741
Mph1103I ATGCAT 1 cut(s) 1093
MroI TCCGGA 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 7 cut(s) 762, 1175, 1191, 1265, 1269, 1572, 1972
MslI CAYNNNNRTG 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 1165
MspI CCGG 1 cut(s) 1220
MspR9I CCNGG 1 cut(s) 428
MunI CAATTG 1 cut(s) 468
Mva1269I GAATGC 5 cut(s) 507, 604, 724, 729, 1206
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 5 cut(s) 111, 223, 608, 1562, 1613
NdeI CATATG 1 cut(s) 1947
NheI GCTAGC 3 cut(s) 550, 691, 1006
NlaIII CATG 6 cut(s) 407, 889, 1001, 1161, 1409, 1669
NlaIV GGNNCC 3 cut(s) 955, 1780, 1906
NmuCI GTSAC 3 cut(s) 739, 1278, 1312
NsiI ATGCAT 1 cut(s) 1093
NspV TTCGAA 2 cut(s) 169, 1198
PacI TTAATTAA 1 cut(s) 1269
PagI TCATGA 1 cut(s) 885
PaqCI CACCTGC 1 cut(s) 977
PciSI GCTCTTC 2 cut(s) 166, 1326
PctI GAATGC 5 cut(s) 507, 604, 724, 729, 1206
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 8 cut(s) 1030, 1057, 1082, 1195, 1216, 1289, 1297, 1636
PflMI CCANNNNNTGG 1 cut(s) 433
PleI GAGTC 3 cut(s) 364, 1350, 1381
PpsI GAGTC 3 cut(s) 364, 1350, 1381
PpuMI RGGWCCY 1 cut(s) 787
PshAI GACNNNNGTC 1 cut(s) 1617
Psp124BI GAGCTC 3 cut(s) 178, 290, 1918
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 949
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 955, 1780, 1906
PspPI GGNCC 4 cut(s) 559, 787, 803, 1605
PspPPI RGGWCCY 1 cut(s) 787
PsuI RGATCY 3 cut(s) 462, 1395, 1516
PvuII CAGCTG 1 cut(s) 1165
RsaI GTAC 2 cut(s) 257, 1479
RsaNI GTAC 2 cut(s) 256, 1478
RseI CAYNNNNRTG 1 cut(s) 48
SacI GAGCTC 3 cut(s) 178, 290, 1918
SapI GCTCTTC 2 cut(s) 166, 1326
SaqAI TTAA 7 cut(s) 762, 1175, 1191, 1265, 1269, 1572, 1972
Sau96I GGNCC 4 cut(s) 559, 787, 803, 1605
ScaI AGTACT 2 cut(s) 257, 1479
SchI GAGTC 3 cut(s) 364, 1350, 1382
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 4 cut(s) 178, 290, 1918, 1965
SfaNI GCATC 3 cut(s) 1543, 1712, 1888
SfuI TTCGAA 2 cut(s) 169, 1198
SinI GGWCC 3 cut(s) 559, 787, 803
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 4 cut(s) 193, 335, 1025, 1646
SmoI CTYRAG 4 cut(s) 193, 335, 1025, 1646
SsiI CCGC 5 cut(s) 5, 10, 111, 1248, 1628
SspMI CTAG 8 cut(s) 551, 626, 692, 1007, 1422, 1602, 1817, 1896
SstI GAGCTC 3 cut(s) 178, 290, 1918
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 148, 1312
TaqI TCGA 5 cut(s) 169, 798, 1198, 1334, 1720
TatI WGTACW 2 cut(s) 255, 1477
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 8 cut(s) 1030, 1057, 1082, 1195, 1216, 1289, 1297, 1636
Tru1I TTAA 7 cut(s) 762, 1175, 1191, 1265, 1269, 1572, 1972
Tru9I TTAA 7 cut(s) 762, 1175, 1191, 1265, 1269, 1572, 1972
TscAI CASTG 4 cut(s) 748, 836, 1317, 1567
TseFI GTSAC 3 cut(s) 739, 1278, 1312
TseI GCWGC 9 cut(s) 114, 223, 1069, 1402, 1436, 1439, 1556, 1853, 1942
Tsp45I GTSAC 3 cut(s) 739, 1278, 1312
TspDTI ATGAA 7 cut(s) 114, 392, 1146, 1431, 1628, 1689, 1735
TspRI CASTG 4 cut(s) 748, 836, 1317, 1567
Van91I CCANNNNNTGG 1 cut(s) 433
VpaK11BI GGWCC 3 cut(s) 559, 787, 803
XapI RAATTY 6 cut(s) 1015, 1116, 1254, 1327, 1429, 1500
XbaI TCTAGA 1 cut(s) 1816
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 8 cut(s) 551, 626, 692, 1007, 1422, 1602, 1817, 1896
ZrmI AGTACT 2 cut(s) 257, 1479
Zsp2I ATGCAT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.