Rh6DG241000

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004467
Physical Location & Seq
Forward (+)
42786258 .. 42789928
3671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG241000.1

Sequence Viewer

Length: 1479 bp
ATGCGAATGCAACTCAAACGAGCAAAGAAACGGGCAGATACACAGGATATAGAGCTGGCAATGGACCTGATGGTTGTGTTCTCTAAGAAAAATGATGACAGGAATGCAGATAAAGCGATACTTGAAAGACTAGCCAACAAGTTGGAACTACACACTATTGCAGACTTGAAAGAGGAAACAATAGCTGTTAGAAAGCTAGCTAAAAGGAGATCAGCAAGGCAGAATGCTGAAAGCATTCAACAGGTCACTGATCTTTTGGGAAGGTTTAAAGAAAATGCAGGGATATATGAGGACTTCTTGCTCGATGGTCCTGTTTCCACGAGAAATCTGAGGCAGTGTCGGTCTTTGTTGATCCCCAATGAGTTTCTATGCCCGATTACGTTGGAGATTATGACTGATCCTGTTATTGTTGCAACTGGACAGACATATGAAAGAGCAAGCATACAAAAGTGGCTGGGTTCCAATCACCACACCTGCCCAAAGACGGGGCAAACATTGGATCATGTATCGCTAGCACCAAATTTTGCTCTCAAGAATCTAATCCAGCAATGGTGTGAGAAGAATCATTTTGAGCTGCCCAAGAAGGATTCTAATGCATTTTCTGATGGCTCTACTGCTGAAATTTTAGAGGAAATATCTTCCTTAGTGTATGATCTATCTTCATGTCAGCTGGATGTTTTAAAAGAAGCCATTTTTAAGATTCGAATGCTCTCTAAAGAGAATCCCGAGAATAGAGTTCTGATAGCTAAGGGTGGAGGGATTCCCCAGTTGATTAAGCTCTTGTCACATCCAGATTCCAAGATTCAAGAACACACAGTGACAGCTCTTCTAAATTTATCGATTGATGAGACCAACAAAAGACTCATAGCAGGGAAAGAGTCTATTCGTGCTATAATTGAGATCTTGCAGCATGGAACAGATGAAGCTAGAGAGAATTCTGCTGCTGCATTGTTCAGCTTATCAATGCTCCATGAGAACAAAGTACTTGTTGGGACTTTGAATGGAATTCCTCCTTTGGTAGATCTTTTGCAGAATGGAACAACCAGAGGTAGAAAGGATGCTGCCACTGCATTATTTAACTTGTCTTTGAACCAAGCCAACAAGTCTAGGGCCATTGACGCAGGTCTAATACCACCCTTGATTCATCTACTTGAGGATAAGAACTTGGGCATGACTGATGAAGCCCTCTCAATTTTGTTACTCCTTGCATCACATCCTGAGGGTCGAAACGAGATTGGGAGATTATCTTTCATTGAAACTCTGGTTGGAATTATCAAGAGTGGAACCCCCAAGAACAAGGAATGTGCTACATCGGTTCTTCTAGAGCTCGGATTGAACAATTCGTCTTTCACTTTGGCTGCACTTCAGTATGGTGTGTATGAGCATCTGGTGGAGGTGGCTAGATGTGGAACCAACAGAGCTCAAAGGAAAGCAAACTCCCTTTTGCAGCATATGAGTAAATGTGAGCACATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

492

Amino Acids

54.64

Weight (kDa)

7.64

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 118 - 188 8.2e-24 U-box domain
Arm_2 PF04826 211 - 374 6e-06 Armadillo-like
ARM_PUB PF25598 212 - 486 1.1e-24 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 226 - 387 8.9e-10 ARMC5
Arm PF00514 241 - 280 2.1e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 324 - 488 1.6e-06 ARMC5
Arm PF00514 325 - 362 3.9e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 2 cut(s) 482, 1112
AclWI GGATC 3 cut(s) 346, 392, 507
AcsI RAATTY 5 cut(s) 520, 621, 832, 934, 1005
AcuI CTGAAG 1 cut(s) 1349
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 1 cut(s) 984
AfiI CCNNNNNNNGG 2 cut(s) 484, 485
AgsI TTSAA 8 cut(s) 125, 169, 239, 806, 1000, 1090, 1256, 1336
AjuI GAANNNNNNNTTGG 2 cut(s) 1248, 1280
Alw21I GWGCWC 3 cut(s) 1329, 1423, 1470
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 3 cut(s) 346, 392, 507
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 1 cut(s) 1110
ApeKI GCWGC 7 cut(s) 574, 907, 941, 944, 1061, 1358, 1447
ApoI RAATTY 5 cut(s) 520, 621, 832, 934, 1005
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 3 cut(s) 64, 308, 1110
AsuHPI GGTGA 1 cut(s) 458
AsuII TTCGAA 1 cut(s) 703
AsuNHI GCTAGC 2 cut(s) 196, 511
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 2 cut(s) 64, 308
AxyI CCTNAGG 1 cut(s) 1218
BanII GRGCYC 2 cut(s) 1329, 1423
BauI CACGAG 1 cut(s) 319
Bbv12I GWGCWC 3 cut(s) 1329, 1423, 1470
BbvI GCAGC 7 cut(s) 561, 919, 928, 931, 1048, 1345, 1459
BccI CCATC 3 cut(s) 64, 299, 599
BcoDI GTCTC 1 cut(s) 842
BfaI CTAG 7 cut(s) 131, 197, 512, 927, 1107, 1322, 1401
BfuAI ACCTGC 2 cut(s) 482, 1112
BglII AGATCT 2 cut(s) 900, 1021
BisI GCNGC 7 cut(s) 575, 908, 942, 945, 1062, 1359, 1448
BlsI GCNGC 7 cut(s) 576, 909, 943, 946, 1063, 1360, 1449
BmcAI AGTACT 1 cut(s) 984
Bme18I GGWCC 2 cut(s) 64, 308
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 3 cut(s) 64, 308, 1110
BmiI GGNNCC 3 cut(s) 460, 1285, 1411
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 3 cut(s) 1048, 1217, 1393
BmtI GCTAGC 2 cut(s) 200, 515
BmuI ACTGGG 1 cut(s) 760
BoxI GACNNNNGTC 1 cut(s) 1122
Bpu10I CCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 703
BpuEI CTTGAG 2 cut(s) 515, 1172
Bsa29I ATCGAT 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 842
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 485
Bse1I ACTGG 2 cut(s) 421, 766
Bse21I CCTNAGG 1 cut(s) 1218
Bse3DI GCAATG 2 cut(s) 66, 554
BseCI ATCGAT 1 cut(s) 839
BseGI GGATG 4 cut(s) 679, 787, 1063, 1213
BseLI CCNNNNNNNGG 2 cut(s) 484, 485
BseMI GCAATG 2 cut(s) 66, 554
BseMII CTCAG 2 cut(s) 320, 1209
BseNI ACTGG 2 cut(s) 421, 766
BseXI GCAGC 7 cut(s) 561, 919, 928, 931, 1048, 1345, 1459
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 1344
BshFI GGCC 1 cut(s) 1112
BshVI ATCGAT 1 cut(s) 839
BsiHKAI GWGCWC 3 cut(s) 1329, 1423, 1470
BsiHKCI CYCGRG 1 cut(s) 725
BslFI GGGAC 1 cut(s) 1006
BslI CCNNNNNNNGG 2 cut(s) 484, 485
BsmAI GTCTC 1 cut(s) 842
BsmFI GGGAC 1 cut(s) 1006
BsmI GAATGC 5 cut(s) 12, 109, 229, 234, 711
BsnI GGCC 1 cut(s) 1112
Bso31I GGTCTC 1 cut(s) 842
BsoBI CYCGRG 1 cut(s) 725
Bsp119I TTCGAA 1 cut(s) 703
Bsp1286I GDGCHC 3 cut(s) 1329, 1423, 1470
Bsp143I GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
BspANI GGCC 1 cut(s) 1112
BspCNI CTCAG 2 cut(s) 321, 1210
BspDI ATCGAT 1 cut(s) 839
BspLI GGNNCC 3 cut(s) 460, 1285, 1411
BspMI ACCTGC 2 cut(s) 482, 1112
BspOI GCTAGC 2 cut(s) 200, 515
BspPI GGATC 3 cut(s) 346, 392, 507
BspQI GCTCTTC 1 cut(s) 831
BspT104I TTCGAA 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 842
BsrDI GCAATG 2 cut(s) 66, 554
BsrI ACTGG 2 cut(s) 421, 766
BssMI GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
BssSI CACGAG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 817
Bst6I CTCTTC 1 cut(s) 831
BstBI TTCGAA 1 cut(s) 703
BstC8I GCNNGC 4 cut(s) 57, 198, 439, 513
BstDEI CTNAG 5 cut(s) 84, 329, 643, 747, 1218
BstF5I GGATG 4 cut(s) 679, 787, 1063, 1213
BstKTI GATC 8 cut(s) 212, 253, 354, 400, 502, 655, 903, 1024
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 113, 1067, 1118
BstPAI GACNNNNGTC 1 cut(s) 1122
BstV1I GCAGC 7 cut(s) 561, 919, 928, 931, 1048, 1345, 1459
BstX2I RGATCY 2 cut(s) 900, 1021
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 2 cut(s) 900, 1021
Bsu15I ATCGAT 1 cut(s) 839
Bsu36I CCTNAGG 1 cut(s) 1218
BsuRI GGCC 1 cut(s) 1112
BsuTUI ATCGAT 1 cut(s) 839
BtsCI GGATG 4 cut(s) 679, 787, 1063, 1213
BtsI GCAGTG 2 cut(s) 341, 1065
BtsIMutI CAGTG 4 cut(s) 246, 341, 822, 1065
BveI ACCTGC 2 cut(s) 482, 1112
Cac8I GCNNGC 4 cut(s) 57, 198, 439, 513
Cfr13I GGNCC 3 cut(s) 64, 308, 1110
ClaI ATCGAT 1 cut(s) 839
CseI GACGC 1 cut(s) 1127
Csp6I GTAC 1 cut(s) 983
CviAII CATG 5 cut(s) 503, 663, 911, 971, 1171
CviQI GTAC 1 cut(s) 983
DdeI CTNAG 5 cut(s) 84, 329, 643, 747, 1218
DpnI GATC 8 cut(s) 211, 252, 353, 399, 501, 654, 902, 1023
DpnII GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
DraI TTTAAA 2 cut(s) 268, 681
DraIII CACNNNGTG 1 cut(s) 817
Eam1104I CTCTTC 1 cut(s) 831
EarI CTCTTC 1 cut(s) 831
Ecl136II GAGCTC 2 cut(s) 1327, 1421
Eco24I GRGCYC 2 cut(s) 1329, 1423
Eco31I GGTCTC 1 cut(s) 842
Eco47I GGWCC 2 cut(s) 64, 308
Eco53kI GAGCTC 2 cut(s) 1327, 1421
Eco57I CTGAAG 1 cut(s) 1349
Eco81I CCTNAGG 1 cut(s) 1218
Eco88I CYCGRG 1 cut(s) 725
EcoICRI GAGCTC 2 cut(s) 1327, 1421
EcoRI GAATTC 2 cut(s) 934, 1005
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 1329, 1423
FaeI CATG 5 cut(s) 506, 666, 914, 974, 1174
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 1 cut(s) 1006
FatI CATG 5 cut(s) 502, 662, 910, 970, 1170
FauNDI CATATG 2 cut(s) 427, 1452
Fnu4HI GCNGC 7 cut(s) 575, 908, 942, 945, 1062, 1359, 1448
FokI GGATG 4 cut(s) 686, 774, 1070, 1200
FriOI GRGCYC 2 cut(s) 1329, 1423
Fsp4HI GCNGC 7 cut(s) 575, 908, 942, 945, 1062, 1359, 1448
FspBI CTAG 7 cut(s) 131, 197, 512, 927, 1107, 1322, 1401
GluI GCNGC 7 cut(s) 575, 908, 942, 945, 1062, 1359, 1448
GsaI CCCAGC 1 cut(s) 458
HaeIII GGCC 1 cut(s) 1112
HgaI GACGC 1 cut(s) 1127
Hin1II CATG 5 cut(s) 506, 666, 914, 974, 1174
HphI GGTGA 1 cut(s) 458
Hpy188I TCNGA 4 cut(s) 330, 604, 741, 1331
Hpy188III TCNNGA 7 cut(s) 532, 725, 791, 806, 1217, 1276, 1322
HpyAV CCTTC 2 cut(s) 255, 577
HpyCH4III ACNGT 1 cut(s) 817
HpyCH4IV ACGT 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 1067, 1118
HpyF3I CTNAG 5 cut(s) 84, 329, 643, 747, 1218
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 5 cut(s) 506, 666, 914, 974, 1174
Kzo9I GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
LguI GCTCTTC 1 cut(s) 831
LmnI GCTCC 1 cut(s) 972
Lsp1109I GCAGC 7 cut(s) 561, 919, 928, 931, 1048, 1345, 1459
LweI GCATC 3 cut(s) 1048, 1217, 1393
MaeI CTAG 7 cut(s) 131, 197, 512, 927, 1107, 1322, 1401
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 244, 783, 817, 1197
MalI GATC 8 cut(s) 211, 252, 353, 399, 501, 654, 902, 1023
MboI GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
MboII GAAGA 5 cut(s) 571, 630, 651, 818, 1310
MflI RGATCY 2 cut(s) 900, 1021
MhlI GDGCHC 3 cut(s) 1329, 1423, 1470
MluCI AATT 9 cut(s) 520, 621, 832, 894, 934, 1005, 1191, 1269, 1339
MlyI GAGTC 2 cut(s) 855, 887
MmeI TCCRAC 3 cut(s) 123, 363, 1246
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 6 cut(s) 267, 680, 696, 774, 1077, 1477
MspA1I CMGCKG 1 cut(s) 670
Mva1269I GAATGC 5 cut(s) 12, 109, 229, 234, 711
MwoI GCNNNNNNNGC 3 cut(s) 113, 1067, 1118
NdeI CATATG 2 cut(s) 427, 1452
NdeII GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
NheI GCTAGC 2 cut(s) 196, 511
NlaIII CATG 5 cut(s) 506, 666, 914, 974, 1174
NlaIV GGNNCC 3 cut(s) 460, 1285, 1411
NmuCI GTSAC 3 cut(s) 244, 783, 817
NsiI ATGCAT 1 cut(s) 598
NspV TTCGAA 1 cut(s) 703
PaqCI CACCTGC 1 cut(s) 482
PciSI GCTCTTC 1 cut(s) 831
PctI GAATGC 5 cut(s) 12, 109, 229, 234, 711
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 9 cut(s) 535, 562, 587, 700, 721, 760, 794, 802, 1141
PkrI GCNGC 7 cut(s) 576, 909, 943, 946, 1063, 1360, 1449
PleI GAGTC 2 cut(s) 855, 886
PpsI GAGTC 2 cut(s) 855, 886
PshAI GACNNNNGTC 1 cut(s) 1122
Psp124BI GAGCTC 2 cut(s) 1329, 1423
PspFI CCCAGC 1 cut(s) 454
PspN4I GGNNCC 3 cut(s) 460, 1285, 1411
PspPI GGNCC 3 cut(s) 64, 308, 1110
PsuI RGATCY 2 cut(s) 900, 1021
PvuII CAGCTG 1 cut(s) 670
RsaI GTAC 1 cut(s) 984
RsaNI GTAC 1 cut(s) 983
SacI GAGCTC 2 cut(s) 1329, 1423
SapI GCTCTTC 1 cut(s) 831
SaqAI TTAA 6 cut(s) 267, 680, 696, 774, 1077, 1477
SatI GCNGC 7 cut(s) 575, 908, 942, 945, 1062, 1359, 1448
Sau3AI GATC 8 cut(s) 209, 250, 351, 397, 499, 652, 900, 1021
Sau96I GGNCC 3 cut(s) 64, 308, 1110
ScaI AGTACT 1 cut(s) 984
SchI GAGTC 2 cut(s) 855, 887
SduI GDGCHC 3 cut(s) 1329, 1423, 1470
SfaNI GCATC 3 cut(s) 1048, 1217, 1393
SfuI TTCGAA 1 cut(s) 703
SinI GGWCC 2 cut(s) 64, 308
SmlI CTYRAG 2 cut(s) 530, 1151
SmoI CTYRAG 2 cut(s) 530, 1151
Sse9I AATT 9 cut(s) 520, 621, 832, 894, 934, 1005, 1191, 1269, 1339
SspMI CTAG 7 cut(s) 131, 197, 512, 927, 1107, 1322, 1401
SstI GAGCTC 2 cut(s) 1329, 1423
TaaI ACNGT 1 cut(s) 817
TaiI ACGT 1 cut(s) 383
TaqI TCGA 4 cut(s) 303, 703, 839, 1225
TaqII GACCGA 1 cut(s) 330
TasI AATT 9 cut(s) 520, 621, 832, 894, 934, 1005, 1191, 1269, 1339
TatI WGTACW 1 cut(s) 982
TfiI GAWTC 9 cut(s) 535, 562, 587, 700, 721, 760, 794, 802, 1141
Tru1I TTAA 6 cut(s) 267, 680, 696, 774, 1077, 1477
Tru9I TTAA 6 cut(s) 267, 680, 696, 774, 1077, 1477
TscAI CASTG 4 cut(s) 253, 341, 822, 1072
TseFI GTSAC 3 cut(s) 244, 783, 817
TseI GCWGC 7 cut(s) 574, 907, 941, 944, 1061, 1358, 1447
Tsp45I GTSAC 3 cut(s) 244, 783, 817
TspDTI ATGAA 6 cut(s) 444, 651, 936, 1133, 1194, 1240
TspRI CASTG 4 cut(s) 253, 341, 822, 1072
VpaK11BI GGWCC 2 cut(s) 64, 308
XapI RAATTY 5 cut(s) 520, 621, 832, 934, 1005
XbaI TCTAGA 1 cut(s) 1321
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 7 cut(s) 131, 197, 512, 927, 1107, 1322, 1401
ZrmI AGTACT 1 cut(s) 984
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.