Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004458

Physical Location & Seq

Genomic Coordinates Forward (+)

15947719 .. 15950491

Gene Structure 2773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g18630.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGTTGGCTCCAATGGCGTCAGGATCGTCCGCTCCCTCGCTCGTCGCTCTCTCTCCTCCTCCTCCAAAACCCTCCAAAACCCTAAACCTTCCTCCTCCGTATACACCAGACTCCATGCCCCTGGTGCTTATAGAACCTTCCACTCTGGACTTTGCAGCAATCACAGGGTTTTAGGAAATCATAATGCTTGTAGTAAGAATTGGTTTCTTGGAGGTGTGCATGCTAATTGGACCACAGTGAAGTCGATTCATGGAACTGCATCAGTGGCTGCTTGCGATTACTATGACACACTTGGTGTAAGCAAGACTGCAAGTGTGTCAGAGATAAAGAAAGCCTATTATGGCCTGGCAAAGAAGCTCCATCCAGACACAAATAAAGACGATCCTGATGCTGAAAAGAAGTTTCAAGAAATTCAGAAGGCTTATGAAGTTTTGAAAGACGAGGAGAAGCGTAGAGAGTATGATGAGTACGGCCATGATGGATTTGAACAGAGAGGCAATGGCAACGGCTTTCCTGGTGGTTTCGGCAATCCGTTTGAAGACTTGTTTGGCCGACGGGGTGGTGGACAGGATGTAAATGTAGTTTTGGAGATATCCTTTATGGAAGCTGTTAAGGGATGTGCTAAAACTCTGCAATTTAGAGCTGCAGTTTCCTGTGAAGCTTGTGGTGGAACTGGTGCTCCTCCTGGCACCAAACTTGAATCATGTAAGGGCTGTGGGGGGTTAGGTATGACATCCATGCAAACTGCCCTTGGTTGGATGCATATGCATTGTCCTCAGTGTAAAGGGACTGGCAAAACTTTCCCGATATTGTGCAACTCTTGCAATGGGAAGCGTGTCTTAAATGGAAGCAAGACAGTAAAGTTAGATATAATGGCAGGAATAGACAACAATGATATCATGCGGGTGTTCAGTAACGGTGGAGCTGATCCTGATGGTAATCAACCTGGTGATCTATATGTTACCATTAAGGTTAGGGAAGACCCAGTTTTCCGGAGACAAGGTGCCGACATTCATGTAGATTCCGTTCTGAGTATCTCTCAGGCAATATTGGGAGGAACTATAAAGGTGCCCACTCTCACTGGAGATGTACTCCTAAAGGTCAGCCCTGCCACACAACCTGGTCAAAAGGTAGTCCTGAAGAACAAGGGGATCAAGACAAGGAACTCTTATTCATTGGGTGATCAATATGTGCACTTCAATGTCAGCATCCCAACAAGTCTGTCTCAAAGACAACGTGAAATAATCCAAGAATTTTCCAAAGAAGCTTCTTCTAAAGAAGAACAGGAGGAGGTACACGGTAAGCGTGCTGCTACAGGGGCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000740 GO:0000741 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006457 GO:0006458 GO:0006996 GO:0006997 GO:0007275 GO:0007349 GO:0008150 GO:0008219 GO:0009553 GO:0009558 GO:0009559 GO:0009561 GO:0009653 GO:0009856 GO:0009987 GO:0010197 GO:0010198 GO:0010623 GO:0012501 GO:0016043 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048229 GO:0048284 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051084 GO:0051085 GO:0051704 GO:0061024 GO:0061025 GO:0061077 GO:0071840 GO:0090174

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

443

Amino Acids

47.78

Weight (kDa)

9.05

Isoelectric Point (pI)

40.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	91 - 153	2.3e-27	DnaJ domain
DnaJ_C	PF01556	191 - 403	1.2e-40	DnaJ C terminal domain
DnaJ_CXXCXGXG	PF00684	217 - 276	7.1e-10	DnaJ central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004458)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48030
fragaria_vesca	FvH4_2g18630 FvH4_2g18630 FvH4_7g00600 FvH4_7g00600 FvH4_7g00600
malus_domestica	MD02G1316500.v1.1
prunus_persica	Prupe.2G007700_v2.0.a1
pyrus_communis	pycom02g26520
rosa_chinensis	RchiOBHm_Chr1g0314781 RchiOBHm_Chr6g0284191
rosa_laevigata	RLG00000012758 RLG00000030764
rosa_multiflora	Rmu_sc0002833.1_g000038 Rmu_sc0003778.1_g000002
rosa_roxburghii	Rroxscaffold_4G00331880 Rroxscaffold_7G00184800
rosa_rugosa	Rorug01G0001800 Rorug01G0001800 Rorug06G0161800
rosa_samantha	Rh1AG012700 Rh1BG004300 Rh1CG011200 Rh1DG006000 Rh6AG274600 Rh6BG275100 Rh6CG064000 Rh6CG276800 Rh6DG061100 Rh6DG269400
rosa_wichuraiana	Rw1G000530 Rw6G023620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	689, 1004, 1069
AccBSI	CCGCTC	1 cut(s)	33
AccI	GTMKAC	1 cut(s)	102
AccIII	TCCGGA	1 cut(s)	993
AciI	CCGC	2 cut(s)	31, 904
AclWI	GGATC	4 cut(s)	32, 377, 923, 1160
AcoI	YGGCCR	2 cut(s)	472, 550
AcsI	RAATTY	2 cut(s)	411, 1253
AcuI	CTGAAG	1 cut(s)	1160
AcyI	GRCGYC	1 cut(s)	18
AdeI	CACNNNGTG	1 cut(s)	296
AfaI	GTAC	3 cut(s)	470, 1092, 1296
AfiI	CCNNNNNNNGG	1 cut(s)	756
AgsI	TTSAA	6 cut(s)	407, 436, 488, 539, 701, 1201
AjnI	CCWGG	6 cut(s)	121, 345, 514, 685, 946, 1120
AjuI	GAANNNNNNNTTGG	2 cut(s)	531, 563
AloI	GAACNNNNNNTCC	2 cut(s)	664, 696
AluBI	AGCT	6 cut(s)	358, 608, 644, 662, 926, 1268
AluI	AGCT	6 cut(s)	358, 608, 644, 662, 926, 1268
Alw21I	GWGCWC	2 cut(s)	682, 1197
Alw26I	GTCTC	2 cut(s)	991, 1230
Alw44I	GTGCAC	1 cut(s)	1193
AlwI	GGATC	4 cut(s)	32, 377, 923, 1160
AlwNI	CAGNNNCTG	1 cut(s)	269
Aor13HI	TCCGGA	1 cut(s)	993
AoxI	GGCC	3 cut(s)	343, 472, 550
ApaLI	GTGCAC	1 cut(s)	1193
ApeKI	GCWGC	4 cut(s)	156, 269, 644, 1310
ApoI	RAATTY	2 cut(s)	411, 1253
AspS9I	GGNCC	1 cut(s)	231
AsuHPI	GGTGA	2 cut(s)	962, 1193
AvaII	GGWCC	1 cut(s)	231
BaeGI	GKGCMC	2 cut(s)	1074, 1197
BanI	GGYRCC	3 cut(s)	689, 1004, 1069
BbsI	GAAGAC	2 cut(s)	546, 987
Bbv12I	GWGCWC	2 cut(s)	682, 1197
BbvI	GCAGC	4 cut(s)	168, 256, 631, 1297
BccI	CCATC	3 cut(s)	369, 473, 929
BceAI	ACGGC	2 cut(s)	487, 523
BcgI	CGANNNNNNTGC	2 cut(s)	371, 405
BciT130I	CCWGG	6 cut(s)	123, 347, 516, 687, 948, 1122
BclI	TGATCA	1 cut(s)	1183
BcoDI	GTCTC	2 cut(s)	991, 1230
BfmI	CTRYAG	2 cut(s)	645, 1314
BisI	GCNGC	4 cut(s)	157, 270, 645, 1311
BlsI	GCNGC	4 cut(s)	158, 271, 646, 1312
Bme1390I	CCNGG	6 cut(s)	123, 347, 516, 687, 948, 1122
Bme18I	GGWCC	1 cut(s)	231
BmgT120I	GGNCC	1 cut(s)	231
BmiI	GGNNCC	4 cut(s)	10, 691, 1006, 1071
BmrFI	CCNGG	6 cut(s)	123, 347, 516, 687, 948, 1122
BmrI	ACTGGG	1 cut(s)	980
BmsI	GCATC	4 cut(s)	269, 379, 750, 1218
BmuI	ACTGGG	1 cut(s)	980
BpiI	GAAGAC	2 cut(s)	546, 987
BplI	GAGNNNNNCTC	4 cut(s)	1024, 1056, 1077, 1109
BpmI	CTGGAG	1 cut(s)	1104
BsaBI	GATNNNNATC	1 cut(s)	939
BsaHI	GRCGYC	1 cut(s)	18
BsaJI	CCNNGG	2 cut(s)	121, 751
BsaWI	WCCGGW	1 cut(s)	993
BsaXI	ACNNNNNCTCC	4 cut(s)	664, 694, 988, 1018
Bsc4I	CCNNNNNNNGG	1 cut(s)	756
Bse1I	ACTGG	4 cut(s)	679, 796, 986, 1087
Bse3DI	GCAATG	2 cut(s)	505, 832
Bse8I	GATNNNNATC	1 cut(s)	939
BseAI	TCCGGA	1 cut(s)	993
BseBI	CCWGG	6 cut(s)	123, 347, 516, 687, 948, 1122
BseDI	CCNNGG	2 cut(s)	121, 751
BseGI	GGATG	6 cut(s)	361, 577, 623, 734, 765, 1209
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	1 cut(s)	756
BseMI	GCAATG	2 cut(s)	505, 832
BseMII	CTCAG	3 cut(s)	791, 1022, 1055
BseNI	ACTGG	4 cut(s)	679, 796, 986, 1087
BseRI	GAGGAG	7 cut(s)	46, 49, 52, 85, 458, 672, 1304
BseSI	GKGCMC	2 cut(s)	1074, 1197
BseXI	GCAGC	4 cut(s)	168, 256, 631, 1297
BshFI	GGCC	3 cut(s)	345, 474, 552
BshNI	GGYRCC	3 cut(s)	689, 1004, 1069
BsiHKAI	GWGCWC	2 cut(s)	682, 1197
BsiSI	CCGG	1 cut(s)	994
BslFI	GGGAC	1 cut(s)	802
BslI	CCNNNNNNNGG	1 cut(s)	756
BsmAI	GTCTC	2 cut(s)	991, 1230
BsmFI	GGGAC	1 cut(s)	802
BsnI	GGCC	3 cut(s)	345, 474, 552
Bsp1286I	GDGCHC	3 cut(s)	682, 1074, 1197
Bsp13I	TCCGGA	1 cut(s)	993
Bsp143I	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
BspACI	CCGC	2 cut(s)	31, 904
BspANI	GGCC	3 cut(s)	345, 474, 552
BspCNI	CTCAG	3 cut(s)	790, 1023, 1054
BspEI	TCCGGA	1 cut(s)	993
BspLI	GGNNCC	4 cut(s)	10, 691, 1006, 1071
BspMAI	CTGCAG	1 cut(s)	649
BspPI	GGATC	4 cut(s)	32, 377, 923, 1160
BspT107I	GGYRCC	3 cut(s)	689, 1004, 1069
BsrBI	CCGCTC	1 cut(s)	33
BsrDI	GCAATG	2 cut(s)	505, 832
BsrI	ACTGG	4 cut(s)	679, 796, 986, 1087
BssECI	CCNNGG	2 cut(s)	121, 751
BssMI	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
BssNAI	GTATAC	1 cut(s)	103
BssNI	GRCGYC	1 cut(s)	18
BssT1I	CCWWGG	1 cut(s)	751
Bst1107I	GTATAC	1 cut(s)	103
Bst2UI	CCWGG	6 cut(s)	123, 347, 516, 687, 948, 1122
Bst4CI	ACNGT	4 cut(s)	238, 859, 920, 1301
BstACI	GRCGYC	1 cut(s)	18
BstAPI	GCANNNNNTGC	1 cut(s)	822
BstC8I	GCNNGC	3 cut(s)	222, 274, 1308
BstDEI	CTNAG	3 cut(s)	777, 1031, 1041
BstF5I	GGATG	6 cut(s)	361, 577, 623, 734, 765, 1209
BstKTI	GATC	6 cut(s)	27, 385, 931, 955, 1155, 1186
BstMAI	GTCTC	2 cut(s)	991, 1230
BstMBI	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
BstMWI	GCNNNNNNNGC	5 cut(s)	15, 125, 266, 822, 1319
BstNI	CCWGG	6 cut(s)	123, 347, 516, 687, 948, 1122
BstNSI	RCATGY	1 cut(s)	224
BstSCI	CCNGG	6 cut(s)	121, 345, 514, 685, 946, 1120
BstSFI	CTRYAG	2 cut(s)	645, 1314
BstSLI	GKGCMC	2 cut(s)	1074, 1197
BstV1I	GCAGC	4 cut(s)	168, 256, 631, 1297
BstV2I	GAAGAC	2 cut(s)	546, 987
BstXI	CCANNNNNNTGG	1 cut(s)	122
BstZ17I	GTATAC	1 cut(s)	103
BsuRI	GGCC	3 cut(s)	345, 474, 552
BtsCI	GGATG	6 cut(s)	361, 577, 623, 734, 765, 1209
BtsIMutI	CAGTG	4 cut(s)	243, 270, 785, 1080
Cac8I	GCNNGC	3 cut(s)	222, 274, 1308
CaiI	CAGNNNCTG	1 cut(s)	269
Cfr13I	GGNCC	1 cut(s)	231
CseI	GACGC	1 cut(s)	7
CsiI	ACCWGGT	2 cut(s)	946, 1120
Csp6I	GTAC	3 cut(s)	469, 1091, 1295
CviAII	CATG	8 cut(s)	116, 221, 251, 476, 705, 739, 901, 1016
CviQI	GTAC	3 cut(s)	469, 1091, 1295
DdeI	CTNAG	3 cut(s)	777, 1031, 1041
DpnI	GATC	6 cut(s)	26, 384, 930, 954, 1154, 1185
DpnII	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
DraIII	CACNNNGTG	1 cut(s)	296
EaeI	YGGCCR	2 cut(s)	472, 550
Eco130I	CCWWGG	1 cut(s)	751
Eco32I	GATATC	2 cut(s)	594, 898
Eco47I	GGWCC	1 cut(s)	231
Eco57I	CTGAAG	1 cut(s)	1160
EcoRII	CCWGG	6 cut(s)	121, 345, 514, 685, 946, 1120
EcoRV	GATATC	2 cut(s)	594, 898
EcoT14I	CCWWGG	1 cut(s)	751
EcoT22I	ATGCAT	2 cut(s)	765, 771
ErhI	CCWWGG	1 cut(s)	751
FaeI	CATG	8 cut(s)	119, 224, 254, 479, 708, 742, 904, 1019
FalI	AAGNNNNNCTT	4 cut(s)	824, 856, 1153, 1185
FaqI	GGGAC	1 cut(s)	802
FatI	CATG	8 cut(s)	115, 220, 250, 475, 704, 738, 900, 1015
FauI	CCCGC	1 cut(s)	897
FauNDI	CATATG	1 cut(s)	765
FbaI	TGATCA	1 cut(s)	1183
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	4 cut(s)	157, 270, 645, 1311
FokI	GGATG	6 cut(s)	348, 584, 630, 721, 772, 1196
Fsp4HI	GCNGC	4 cut(s)	157, 270, 645, 1311
GluI	GCNGC	4 cut(s)	157, 270, 645, 1311
GsuI	CTGGAG	1 cut(s)	1104
HaeIII	GGCC	3 cut(s)	345, 474, 552
HapII	CCGG	1 cut(s)	994
HgaI	GACGC	1 cut(s)	7
Hin1I	GRCGYC	1 cut(s)	18
Hin1II	CATG	8 cut(s)	119, 224, 254, 479, 708, 742, 904, 1019
HindIII	AAGCTT	2 cut(s)	660, 1266
HinfI	GANTC	4 cut(s)	111, 247, 701, 1022
HpaII	CCGG	1 cut(s)	994
HphI	GGTGA	2 cut(s)	962, 1193
Hpy166II	GTNNAC	4 cut(s)	103, 566, 1195, 1297
Hpy188I	TCNGA	3 cut(s)	322, 417, 1032
Hpy8I	GTNNAC	4 cut(s)	103, 566, 1195, 1297
Hpy99I	CGWCG	2 cut(s)	48, 558
HpyAV	CCTTC	3 cut(s)	99, 148, 412
HpyCH4III	ACNGT	4 cut(s)	238, 859, 920, 1301
HpyCH4IV	ACGT	1 cut(s)	1237
HpyF10VI	GCNNNNNNNGC	5 cut(s)	15, 125, 266, 822, 1319
HpyF3I	CTNAG	3 cut(s)	777, 1031, 1041
HpySE526I	ACGT	1 cut(s)	1237
Hsp92I	GRCGYC	1 cut(s)	18
Hsp92II	CATG	8 cut(s)	119, 224, 254, 479, 708, 742, 904, 1019
Kpn2I	TCCGGA	1 cut(s)	993
Ksp22I	TGATCA	1 cut(s)	1183
Kzo9I	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
LmnI	GCTCC	5 cut(s)	14, 38, 363, 685, 923
Lsp1109I	GCAGC	4 cut(s)	168, 256, 631, 1297
LweI	GCATC	4 cut(s)	269, 379, 750, 1218
MabI	ACCWGGT	2 cut(s)	946, 1120
MaeII	ACGT	1 cut(s)	1237
MaeIII	GTNAC	2 cut(s)	914, 961
MalI	GATC	6 cut(s)	26, 384, 930, 954, 1154, 1185
MbiI	CCGCTC	1 cut(s)	33
MboI	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
MboII	GAAGA	5 cut(s)	551, 992, 1153, 1263, 1292
MhlI	GDGCHC	3 cut(s)	682, 1074, 1197
MluCI	AATT	5 cut(s)	199, 226, 411, 635, 1253
MlyI	GAGTC	1 cut(s)	105
MmeI	TCCRAC	1 cut(s)	737
Mph1103I	ATGCAT	2 cut(s)	765, 771
MroI	TCCGGA	1 cut(s)	993
MseI	TTAA	3 cut(s)	612, 842, 969
MslI	CAYNNNNRTG	2 cut(s)	905, 1200
MspI	CCGG	1 cut(s)	994
MspR9I	CCNGG	6 cut(s)	123, 347, 516, 687, 948, 1122
MvaI	CCWGG	6 cut(s)	123, 347, 516, 687, 948, 1122
MwoI	GCNNNNNNNGC	5 cut(s)	15, 125, 266, 822, 1319
NdeI	CATATG	1 cut(s)	765
NdeII	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
NlaIII	CATG	8 cut(s)	119, 224, 254, 479, 708, 742, 904, 1019
NlaIV	GGNNCC	4 cut(s)	10, 691, 1006, 1071
NsiI	ATGCAT	2 cut(s)	765, 771
NspI	RCATGY	1 cut(s)	224
PaeI	GCATGC	1 cut(s)	224
PfeI	GAWTC	3 cut(s)	247, 701, 1022
PkrI	GCNGC	4 cut(s)	158, 271, 646, 1312
PleI	GAGTC	1 cut(s)	105
PpsI	GAGTC	1 cut(s)	105
Psp6I	CCWGG	6 cut(s)	121, 345, 514, 685, 946, 1120
PspGI	CCWGG	6 cut(s)	121, 345, 514, 685, 946, 1120
PspN4I	GGNNCC	4 cut(s)	10, 691, 1006, 1071
PspPI	GGNCC	1 cut(s)	231
PsrI	GAACNNNNNNTAC	2 cut(s)	1011, 1043
PstI	CTGCAG	1 cut(s)	649
PstNI	CAGNNNCTG	1 cut(s)	269
RsaI	GTAC	3 cut(s)	470, 1092, 1296
RsaNI	GTAC	3 cut(s)	469, 1091, 1295
RseI	CAYNNNNRTG	2 cut(s)	905, 1200
SaqAI	TTAA	3 cut(s)	612, 842, 969
SatI	GCNGC	4 cut(s)	157, 270, 645, 1311
Sau3AI	GATC	6 cut(s)	24, 382, 928, 952, 1152, 1183
Sau96I	GGNCC	1 cut(s)	231
SchI	GAGTC	1 cut(s)	105
ScrFI	CCNGG	6 cut(s)	123, 347, 516, 687, 948, 1122
SduI	GDGCHC	3 cut(s)	682, 1074, 1197
SexAI	ACCWGGT	2 cut(s)	946, 1120
SfaNI	GCATC	4 cut(s)	269, 379, 750, 1218
SfcI	CTRYAG	2 cut(s)	645, 1314
SinI	GGWCC	1 cut(s)	231
SmiMI	CAYNNNNRTG	2 cut(s)	905, 1200
SphI	GCATGC	1 cut(s)	224
Sse9I	AATT	5 cut(s)	199, 226, 411, 635, 1253
SsiI	CCGC	2 cut(s)	31, 904
SspI	AATATT	1 cut(s)	1050
StyD4I	CCNGG	6 cut(s)	121, 345, 514, 685, 946, 1120
StyI	CCWWGG	1 cut(s)	751
TaaI	ACNGT	4 cut(s)	238, 859, 920, 1301
TaiI	ACGT	1 cut(s)	1240
TaqI	TCGA	1 cut(s)	245
TasI	AATT	5 cut(s)	199, 226, 411, 635, 1253
TatI	WGTACW	1 cut(s)	1090
TfiI	GAWTC	3 cut(s)	247, 701, 1022
Tru1I	TTAA	3 cut(s)	612, 842, 969
Tru9I	TTAA	3 cut(s)	612, 842, 969
TscAI	CASTG	4 cut(s)	243, 270, 785, 1087
TseI	GCWGC	4 cut(s)	156, 269, 644, 1310
TspDTI	ATGAA	4 cut(s)	239, 441, 1004, 1164
TspGWI	ACGGA	3 cut(s)	88, 522, 1015
TspRI	CASTG	4 cut(s)	243, 270, 785, 1087
VneI	GTGCAC	1 cut(s)	1193
VpaK11BI	GGWCC	1 cut(s)	231
XapI	RAATTY	2 cut(s)	411, 1253
XceI	RCATGY	1 cut(s)	224
XmiI	GTMKAC	1 cut(s)	102
Zsp2I	ATGCAT	2 cut(s)	765, 771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.