FvH4_2g21290

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006341
Physical Location & Seq
Forward (+)
17718982 .. 17724594
5613 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21290.t1

Sequence Viewer

Length: 2580 bp
ATGGAAAACAATTCGGTTCCACCTGGTTTTGCGCCTAGGACGTCCTTCAAGCTGAAGAAGGCAGAAGTCTCAGTTGTGGGAGTTGAAGGAACCGATAGGTCCAATGCTGTTGTGGATTCTTCCAAACAAGGGTCAACCCAGATGGACTCAACATCTGAAATGACCGCCTTGGAGAAGGTCAAAACATTTCTTCAGCAAAGGCCATGGTTGCTTTTCGACCAAACTGATCACACTTCAGTGCAGCCTGACCCACAACAGTTTCAAATGGAACCCCCTCCAAAAGCTTGCCTCTCAAATGGTGTGACTCGTGGAAGCCCAGAATCCACTTATTGTGTAAAGGAACCCCGCCTCCCGAAAGGTGTTACTCATGGATGTCCAGAATCCGGTTACTGTGTAAAGGTAACAGAAAGGTGGCGTCCTGAGGGTGGAATAATAGAGAAGCTTGATAAGGCTCCTGTTTTCTACCCAACACATGAGGAATTTAAGGACACTCTCACGTATATCACAAGTATAAGTGCAAGAGCAGCGCAATTTGGTATCTGTCGTATTAGTCCTCCTCCTTCCTGGATACCTCCACCTCCTATTGAGGAAAACAGCATATGGAGAAGTTCCACATTTGCTTCCCATATTCAGCGGATTGATGGGTTGCGGAGTAAACTGGACGGCTCTGATGAGAGCAGAAAAAAGAAGAGAAGAATCTCAACAGTGGGTTTGGACTGTGGATTTGAATTTACCTCAAGTATGGGTGAAGCAGGACAGTCTGATGTCAATTGCTTTGAGTCGGAACGTGGTCCAGAATTCACTCTTGACAGTTTTAAGAGGTATGCAGATGATTTCAAGCGTCAGTATTTCCGCAAAAGTGACGTCATAGGTAATCAAGAGCAGTGGGAGCCATCAGTGGAGAATATCGAAGCTGAATATACACGTATCGTTGAAAACCCAACTGAAGGAATCGAGGTGCTTTGTGGAAATTATTTGGACACTAAAGGGCTTGGGAGTGGATTTCCAATAGTATCACCTGATACCCCTGAATGCTTGCAATCTGGCTGGAATTTAAATAATCTACCCAGACTGCCTGGTTCTCTTCTTTCTTTTGAAAGCCATGACACATGTCATATTTTAGTGCCTCAGACTCGTGTAGGAATGTGTTTTTCATCTAGTCTTTGGAGAGTTGAAGAGCACCACTTATACTCATTGTGTTACAATCATCTGGGTGCTCCTAAAGTATGGTATGGTGTCCCTGGGAGTTCCCGTATACGTTTTGAGGCAGCCAAAACGAAGTCCTTTCCAGATTTATTAAAACAGCATAGGCTGGTTAAGCAAGTATCCCCCTCCGCATTGAAATCAGAGGGCACACCTGTCTTTTGTTGCATTCAGCGTCCTGGAGAGTTTGTTCTTGTCTTCCCTGGAGCTTATCATTCAGAATTTGATTGTGGCTTTAATTTTTCTGAAAGTGCATGTTTTGCTCCTCTAGACTGGTTGCCTTATGGACAGAATGCTGTTGAACTTTACTCTGAACGGGAAAGGAAAACATCCATATCCTATGATAAGCTGTTGCTTGGAGCAGCTAGGGAAGCTGTAAGGGCACAATGGGAAATTTCACTGTTGAAGAACAAAAAAACCTCAGAGGGTTTACGCTGGAAAAGTGCCTGCTTTAAGGATGGGATTTTAACAGAACTACTCAAGACGCGTCTCAAGTCTGAAGAGGTTCGAAGGAAATATTTTTGCACCTCTTTGAAGTCACAAAAGATGAATTCAGATTTTGATGCTACCATAAAAAGGGAATGCAGCATTTGTTTCTGCGATCTTCATTTCTCTGCAGTAGGTTGTCCTTGTTCTGTAGACAGATACTCGTGTCTATTTCATGCAAAGCAGCGCTGTTTTTGTGATTGGAGTAACAAGTTTGTCCTCTACCGTCATGAAATCAAGGAGTTAAATCTACTTGTTGAAGCTTTGGAAGGAAAATTAAGTGCAGTCATCAAATGGGCGAAAGATGATCTTGGCCTATATTTGCATGAACATCTTCCCATCTCTCTGACAGATAAAACAAAGCTGAAAGAGTACACATCGGAAGATGCATCTTCACCAGTTAGCACTTGTGACACTGCTTCTAGCATAAAGGCGGAATTGAAGGCGCGTATGCTGCAATCCACTATTTCAAACATGTTGAAAGCAAACCGTGATCCAATAGCATCCACTGATGCTGCTTCTAGCATCAGGGCTGAATTAAAGGCACGTCTCCTGCAGTCTTCAATCTCCAAGACATTGACAGGAAATGATAATCCGCTAGATGCTGCAGCAGCCAATGGCAATGGTAGAAATTTTGTTTCTAGCCAATCAACAATCTCGGATGAACATAAAGGCATGTGCACTCCGCAACAGCCAGTGGCAATGGTAGGAACTCCTATTCTGCCGATAGAGGTGGCCTCTGATGTTTCCTCTGTAACTTCAAGTGAATCATCCTCGGAATCTGATGATATAATACCACATCTTGGCAAGTCTTCAGAATCTAGTCATCAAGCAGAAAGTAGGGCACCTCAGGATTGCGTTCATGTTCTTAGTGATGACAGTGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

860

Amino Acids

95.57

Weight (kDa)

6.12

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 152 - 185 8.8e-14 jmjN domain
JmjC PF02373 380 - 487 2.4e-29 JmjC domain, hydroxylase
zf-C5HC2 PF02928 596 - 646 1.5e-12 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 44, 867
AccB1I GGYRCC 1 cut(s) 2533
AccB7I CCANNNNNTGG 1 cut(s) 2492
AccI GTMKAC 2 cut(s) 1255, 1842
AccII CGCG 2 cut(s) 1690, 2137
AciI CCGC 9 cut(s) 165, 346, 634, 649, 853, 1335, 2123, 2285, 2375
AclWI GGATC 1 cut(s) 2177
AcsI RAATTY 8 cut(s) 479, 728, 797, 1051, 1424, 1596, 1753, 2320
AcuI CTGAAG 6 cut(s) 74, 176, 219, 966, 1722, 2487
AcyI GRCGYC 3 cut(s) 41, 415, 864
AfaI GTAC 1 cut(s) 2063
AfeI AGCGCT 1 cut(s) 1877
AfiI CCNNNNNNNGG 6 cut(s) 129, 383, 425, 947, 1779, 2492
AflIII ACRYGT 4 cut(s) 923, 1109, 1688, 2163
AjiI CACGTC 1 cut(s) 2237
AjnI CCWGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
AleI CACNNNNGTG 1 cut(s) 236
AloI GAACNNNNNNTCC 4 cut(s) 333, 365, 1377, 1409
Alw21I GWGCWC 3 cut(s) 1182, 1219, 2372
Alw26I GTCTC 3 cut(s) 73, 1697, 2243
Alw44I GTGCAC 1 cut(s) 2368
AlwI GGATC 1 cut(s) 2177
Aor51HI AGCGCT 1 cut(s) 1877
AoxI GGCC 3 cut(s) 200, 2002, 2424
ApaLI GTGCAC 1 cut(s) 2368
ApoI RAATTY 8 cut(s) 479, 728, 797, 1051, 1424, 1596, 1753, 2320
ArsI GACNNNNNNTTYG 4 cut(s) 707, 739, 849, 881
Asp700I GAANNNNTTC 2 cut(s) 1707, 2022
AspA2I CCTAGG 1 cut(s) 35
AspLEI GCGC 4 cut(s) 34, 529, 1878, 2137
AspS9I GGNCC 2 cut(s) 99, 791
AsuHPI GGTGA 3 cut(s) 758, 1008, 2076
AsuII TTCGAA 1 cut(s) 1711
AvaII GGWCC 2 cut(s) 99, 791
AvrII CCTAGG 1 cut(s) 35
AxyI CCTNAGG 2 cut(s) 420, 2538
BaeGI GKGCMC 4 cut(s) 1355, 1588, 2372, 2536
BaeI ACNNNNGTAYC 2 cut(s) 1840, 1873
BanI GGYRCC 1 cut(s) 2533
BauI CACGAG 3 cut(s) 306, 1134, 1852
BbsI GAAGAC 3 cut(s) 1393, 2241, 2493
Bbv12I GWGCWC 3 cut(s) 1182, 1219, 2372
BccI CCATC 6 cut(s) 136, 635, 901, 1655, 2036, 2567
BceAI ACGGC 1 cut(s) 679
BciT130I CCWGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
BciVI GTATCC 2 cut(s) 561, 1336
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 3 cut(s) 73, 1697, 2243
BfaI CTAG 9 cut(s) 36, 1158, 1472, 1569, 2112, 2211, 2288, 2331, 2511
BfmI CTRYAG 4 cut(s) 1818, 1839, 2243, 2295
BfoI RGCGCY 1 cut(s) 1879
BfuI GTATCC 2 cut(s) 561, 1336
BlnI CCTAGG 1 cut(s) 35
Bme1390I CCNGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
Bme18I GGWCC 2 cut(s) 99, 791
BmgBI CACGTC 1 cut(s) 2237
BmgT120I GGNCC 2 cut(s) 99, 791
BmiI GGNNCC 7 cut(s) 18, 91, 270, 342, 453, 891, 2535
BmrFI CCNGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
BmsI GCATC 7 cut(s) 1756, 2065, 2087, 2191, 2201, 2223, 2281
BoxI GACNNNNGTC 1 cut(s) 1110
BpiI GAAGAC 3 cut(s) 1393, 2241, 2493
BplI GAGNNNNNCTC 2 cut(s) 2411, 2443
BpmI CTGGAG 2 cut(s) 1404, 1428
Bpu14I TTCGAA 1 cut(s) 1711
BpuEI CTTGAG 3 cut(s) 721, 1667, 1679
BsaAI YACGTR 2 cut(s) 498, 926
BsaHI GRCGYC 3 cut(s) 41, 415, 864
BsaJI CCNNGG 7 cut(s) 35, 168, 203, 1240, 1241, 1405, 2463
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 6 cut(s) 333, 363, 1237, 1267, 1377, 1407
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 383, 425, 947, 1779, 2492
Bse1I ACTGG 4 cut(s) 663, 1481, 2087, 2384
Bse21I CCTNAGG 2 cut(s) 420, 2538
Bse3DI GCAATG 2 cut(s) 2317, 2397
BseBI CCWGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
BseDI CCNNGG 7 cut(s) 35, 168, 203, 1240, 1241, 1405, 2463
BseGI GGATG 6 cut(s) 377, 1532, 1666, 2192, 2356, 2459
BseLI CCNNNNNNNGG 6 cut(s) 129, 383, 425, 947, 1779, 2492
BseMI GCAATG 2 cut(s) 2317, 2397
BseMII CTCAG 5 cut(s) 84, 411, 1142, 1638, 2552
BseNI ACTGG 4 cut(s) 663, 1481, 2087, 2384
BseRI GAGGAG 2 cut(s) 546, 1458
BseSI GKGCMC 4 cut(s) 1355, 1588, 2372, 2536
BsgI GTGCAG 2 cut(s) 260, 1992
Bsh1236I CGCG 2 cut(s) 1690, 2137
BshFI GGCC 3 cut(s) 202, 2004, 2426
BshNI GGYRCC 1 cut(s) 2533
BsiHKAI GWGCWC 3 cut(s) 1182, 1219, 2372
BsiSI CCGG 1 cut(s) 384
BslFI GGGAC 1 cut(s) 1223
BslI CCNNNNNNNGG 6 cut(s) 129, 383, 425, 947, 1779, 2492
BsmAI GTCTC 3 cut(s) 73, 1697, 2243
BsmBI CGTCTC 2 cut(s) 1697, 2243
BsmFI GGGAC 1 cut(s) 1223
BsmI GAATGC 4 cut(s) 1037, 1371, 1501, 1790
BsnI GGCC 3 cut(s) 202, 2004, 2426
Bsp119I TTCGAA 1 cut(s) 1711
Bsp1286I GDGCHC 6 cut(s) 1182, 1219, 1355, 1588, 2372, 2536
Bsp143I GATC 4 cut(s) 226, 1804, 1996, 2182
Bsp19I CCATGG 1 cut(s) 203
BspACI CCGC 9 cut(s) 165, 346, 634, 649, 853, 1335, 2123, 2285, 2375
BspANI GGCC 3 cut(s) 202, 2004, 2426
BspCNI CTCAG 5 cut(s) 83, 412, 1141, 1637, 2551
BspFNI CGCG 2 cut(s) 1690, 2137
BspHI TCATGA 1 cut(s) 1918
BspLI GGNNCC 7 cut(s) 18, 91, 270, 342, 453, 891, 2535
BspMAI CTGCAG 3 cut(s) 1822, 2247, 2299
BspPI GGATC 1 cut(s) 2177
BspQI GCTCTTC 1 cut(s) 1170
BspT104I TTCGAA 1 cut(s) 1711
BspT107I GGYRCC 1 cut(s) 2533
BsrDI GCAATG 2 cut(s) 2317, 2397
BsrI ACTGG 4 cut(s) 663, 1481, 2087, 2384
BssECI CCNNGG 7 cut(s) 35, 168, 203, 1240, 1241, 1405, 2463
BssMI GATC 4 cut(s) 226, 1804, 1996, 2182
BssNAI GTATAC 1 cut(s) 1256
BssNI GRCGYC 3 cut(s) 41, 415, 864
BssSI CACGAG 3 cut(s) 306, 1134, 1852
BssT1I CCWWGG 3 cut(s) 35, 168, 203
Bst1107I GTATAC 1 cut(s) 1256
Bst2BI CACGAG 3 cut(s) 306, 1134, 1852
Bst2UI CCWGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
Bst6I CTCTTC 4 cut(s) 683, 1089, 1170, 1698
BstACI GRCGYC 3 cut(s) 41, 415, 864
BstAPI GCANNNNNTGC 1 cut(s) 1463
BstBAI YACGTR 2 cut(s) 498, 926
BstBI TTCGAA 1 cut(s) 1711
BstC8I GCNNGC 3 cut(s) 286, 1037, 1651
BstDEI CTNAG 6 cut(s) 70, 420, 1128, 1624, 2538, 2558
BstDSI CCRYGG 1 cut(s) 203
BstF5I GGATG 6 cut(s) 377, 1532, 1666, 2192, 2356, 2459
BstFNI CGCG 2 cut(s) 1690, 2137
BstH2I RGCGCY 1 cut(s) 1879
BstHHI GCGC 4 cut(s) 34, 529, 1878, 2137
BstKTI GATC 4 cut(s) 229, 1807, 1999, 2185
BstMAI GTCTC 3 cut(s) 73, 1697, 2243
BstMBI GATC 4 cut(s) 226, 1804, 1996, 2182
BstMWI GCNNNNNNNGC 9 cut(s) 208, 524, 889, 1318, 1463, 1574, 1583, 2143, 2300
BstNI CCWGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
BstNSI RCATGY 4 cut(s) 1113, 1461, 2167, 2368
BstPAI GACNNNNGTC 1 cut(s) 1110
BstSCI CCNGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
BstSFI CTRYAG 4 cut(s) 1818, 1839, 2243, 2295
BstSLI GKGCMC 4 cut(s) 1355, 1588, 2372, 2536
BstUI CGCG 2 cut(s) 1690, 2137
BstV2I GAAGAC 3 cut(s) 1393, 2241, 2493
BstZ17I GTATAC 1 cut(s) 1256
Bsu36I CCTNAGG 2 cut(s) 420, 2538
BsuI GTATCC 2 cut(s) 561, 1336
BsuRI GGCC 3 cut(s) 202, 2004, 2426
BtgI CCRYGG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 2237
BtsCI GGATG 6 cut(s) 377, 1532, 1666, 2192, 2356, 2459
BtsI GCAGTG 2 cut(s) 890, 2103
BtsIMutI CAGTG 9 cut(s) 243, 711, 890, 903, 1601, 2103, 2196, 2391, 2575
Cac8I GCNNGC 3 cut(s) 286, 1037, 1651
CciI TCATGA 1 cut(s) 1918
CfoI GCGC 4 cut(s) 34, 529, 1878, 2137
Cfr13I GGNCC 2 cut(s) 99, 791
CseI GACGC 5 cut(s) 404, 830, 1367, 1679, 1696
CsiI ACCWGGT 1 cut(s) 22
Csp6I GTAC 1 cut(s) 2062
CspCI CAANNNNNGTGG 2 cut(s) 866, 901
CviQI GTAC 1 cut(s) 2062
DdeI CTNAG 6 cut(s) 70, 420, 1128, 1624, 2538, 2558
DpnI GATC 4 cut(s) 228, 1806, 1998, 2184
DpnII GATC 4 cut(s) 226, 1804, 1996, 2182
DraI TTTAAA 1 cut(s) 1056
Eam1104I CTCTTC 4 cut(s) 683, 1089, 1170, 1698
EarI CTCTTC 4 cut(s) 683, 1089, 1170, 1698
EciI GGCGGA 1 cut(s) 2138
Eco130I CCWWGG 3 cut(s) 35, 168, 203
Eco47I GGWCC 2 cut(s) 99, 791
Eco47III AGCGCT 1 cut(s) 1877
Eco57I CTGAAG 6 cut(s) 74, 176, 219, 966, 1722, 2487
Eco81I CCTNAGG 2 cut(s) 420, 2538
EcoRI GAATTC 2 cut(s) 797, 1753
EcoRII CCWGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
EcoT14I CCWWGG 3 cut(s) 35, 168, 203
EcoT22I ATGCAT 1 cut(s) 2080
ErhI CCWWGG 3 cut(s) 35, 168, 203
Esp3I CGTCTC 2 cut(s) 1697, 2243
FalI AAGNNNNNCTT 2 cut(s) 1983, 2015
FaqI GGGAC 1 cut(s) 1223
FauI CCCGC 1 cut(s) 353
FauNDI CATATG 1 cut(s) 599
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 2 cut(s) 1255, 1842
FokI GGATG 6 cut(s) 384, 1519, 1673, 2179, 2363, 2446
FspBI CTAG 9 cut(s) 36, 1158, 1472, 1569, 2112, 2211, 2288, 2331, 2511
GlaI GCGC 4 cut(s) 33, 528, 1877, 2136
GsuI CTGGAG 2 cut(s) 1404, 1428
HaeII RGCGCY 1 cut(s) 1879
HaeIII GGCC 3 cut(s) 202, 2004, 2426
HapII CCGG 1 cut(s) 384
HgaI GACGC 5 cut(s) 404, 830, 1367, 1679, 1696
HhaI GCGC 4 cut(s) 34, 529, 1878, 2137
Hin1I GRCGYC 3 cut(s) 41, 415, 864
Hin6I GCGC 4 cut(s) 32, 527, 1876, 2135
HinP1I GCGC 4 cut(s) 32, 527, 1876, 2135
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HindIII AAGCTT 3 cut(s) 282, 440, 1950
HpaII CCGG 1 cut(s) 384
HphI GGTGA 3 cut(s) 758, 1008, 2076
Hpy166II GTNNAC 7 cut(s) 135, 656, 1256, 1634, 1843, 2064, 2370
Hpy8I GTNNAC 7 cut(s) 135, 656, 1256, 1634, 1843, 2064, 2370
HpyAV CCTTC 9 cut(s) 52, 55, 80, 169, 570, 941, 1707, 1952, 2125
HpyCH4IV ACGT 7 cut(s) 41, 497, 787, 864, 925, 1258, 2236
HpyF10VI GCNNNNNNNGC 9 cut(s) 208, 524, 889, 1318, 1463, 1574, 1583, 2143, 2300
HpyF3I CTNAG 6 cut(s) 70, 420, 1128, 1624, 2538, 2558
HpySE526I ACGT 7 cut(s) 41, 497, 787, 864, 925, 1258, 2236
Hsp92I GRCGYC 3 cut(s) 41, 415, 864
HspAI GCGC 4 cut(s) 32, 527, 1876, 2135
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 4 cut(s) 226, 1804, 1996, 2182
LguI GCTCTTC 1 cut(s) 1170
LmnI GCTCC 6 cut(s) 457, 889, 1222, 1409, 1471, 1562
LweI GCATC 7 cut(s) 1756, 2065, 2087, 2191, 2201, 2223, 2281
MabI ACCWGGT 1 cut(s) 22
MaeI CTAG 9 cut(s) 36, 1158, 1472, 1569, 2112, 2211, 2288, 2331, 2511
MaeII ACGT 7 cut(s) 41, 497, 787, 864, 925, 1258, 2236
MalI GATC 4 cut(s) 228, 1806, 1998, 2184
MboI GATC 4 cut(s) 226, 1804, 1996, 2182
MfeI CAATTG 1 cut(s) 769
MhlI GDGCHC 6 cut(s) 1182, 1219, 1355, 1588, 2372, 2536
MluI ACGCGT 1 cut(s) 1688
MlyI GAGTC 4 cut(s) 140, 298, 788, 1126
MmeI TCCRAC 1 cut(s) 762
Mph1103I ATGCAT 1 cut(s) 2080
MroXI GAANNNNTTC 2 cut(s) 1707, 2022
MslI CAYNNNNRTG 2 cut(s) 236, 1212
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
MunI CAATTG 1 cut(s) 769
Mva1269I GAATGC 4 cut(s) 1037, 1371, 1501, 1790
MvaI CCWGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
MvnI CGCG 2 cut(s) 1690, 2137
MwoI GCNNNNNNNGC 9 cut(s) 208, 524, 889, 1318, 1463, 1574, 1583, 2143, 2300
NcoI CCATGG 1 cut(s) 203
NdeI CATATG 1 cut(s) 599
NdeII GATC 4 cut(s) 226, 1804, 1996, 2182
NlaIV GGNNCC 7 cut(s) 18, 91, 270, 342, 453, 891, 2535
NmuCI GTSAC 4 cut(s) 301, 860, 1740, 2099
NsiI ATGCAT 1 cut(s) 2080
NspI RCATGY 4 cut(s) 1113, 1461, 2167, 2368
NspV TTCGAA 1 cut(s) 1711
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 1 cut(s) 1918
PasI CCCWGGG 1 cut(s) 1241
PciI ACATGT 2 cut(s) 1109, 2163
PciSI GCTCTTC 1 cut(s) 1170
PctI GAATGC 4 cut(s) 1037, 1371, 1501, 1790
PdmI GAANNNNTTC 2 cut(s) 1707, 2022
PfeI GAWTC 8 cut(s) 116, 320, 380, 696, 951, 2456, 2468, 2507
PflMI CCANNNNNTGG 1 cut(s) 2492
PfoI TCCNGGA 2 cut(s) 563, 1381
PleI GAGTC 4 cut(s) 140, 298, 787, 1126
PpsI GAGTC 4 cut(s) 140, 298, 787, 1126
Ppu21I YACGTR 2 cut(s) 498, 926
PscI ACATGT 2 cut(s) 1109, 2163
PshAI GACNNNNGTC 1 cut(s) 1110
Psp6I CCWGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
PspGI CCWGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
PspN4I GGNNCC 7 cut(s) 18, 91, 270, 342, 453, 891, 2535
PspPI GGNCC 2 cut(s) 99, 791
PstI CTGCAG 3 cut(s) 1822, 2247, 2299
RsaI GTAC 1 cut(s) 2063
RsaNI GTAC 1 cut(s) 2062
RseI CAYNNNNRTG 2 cut(s) 236, 1212
SapI GCTCTTC 1 cut(s) 1170
Sau3AI GATC 4 cut(s) 226, 1804, 1996, 2182
Sau96I GGNCC 2 cut(s) 99, 791
SchI GAGTC 4 cut(s) 140, 298, 788, 1126
ScrFI CCNGG 6 cut(s) 24, 565, 1077, 1242, 1383, 1407
SduI GDGCHC 6 cut(s) 1182, 1219, 1355, 1588, 2372, 2536
SexAI ACCWGGT 1 cut(s) 22
SfaNI GCATC 7 cut(s) 1756, 2065, 2087, 2191, 2201, 2223, 2281
SfcI CTRYAG 4 cut(s) 1818, 1839, 2243, 2295
SfuI TTCGAA 1 cut(s) 1711
SinI GGWCC 2 cut(s) 99, 791
SmiI ATTTAAAT 1 cut(s) 1056
SmiMI CAYNNNNRTG 2 cut(s) 236, 1212
SmlI CTYRAG 3 cut(s) 736, 1682, 1694
SmoI CTYRAG 3 cut(s) 736, 1682, 1694
SsiI CCGC 9 cut(s) 165, 346, 634, 649, 853, 1335, 2123, 2285, 2375
SspI AATATT 1 cut(s) 1721
SspMI CTAG 9 cut(s) 36, 1158, 1472, 1569, 2112, 2211, 2288, 2331, 2511
StyD4I CCNGG 6 cut(s) 22, 563, 1075, 1240, 1381, 1405
StyI CCWWGG 3 cut(s) 35, 168, 203
SwaI ATTTAAAT 1 cut(s) 1056
TaiI ACGT 7 cut(s) 44, 500, 790, 867, 928, 1261, 2239
TaqI TCGA 4 cut(s) 216, 909, 954, 1711
TatI WGTACW 1 cut(s) 2061
TfiI GAWTC 8 cut(s) 116, 320, 380, 696, 951, 2456, 2468, 2507
TscAI CASTG 9 cut(s) 243, 711, 890, 903, 1608, 2110, 2203, 2391, 2575
TseFI GTSAC 4 cut(s) 301, 860, 1740, 2099
Tsp45I GTSAC 4 cut(s) 301, 860, 1740, 2099
TspDTI ATGAA 8 cut(s) 1143, 1766, 1799, 1853, 1935, 2031, 2367, 2540
TspRI CASTG 9 cut(s) 243, 711, 890, 903, 1608, 2110, 2203, 2391, 2575
Van91I CCANNNNNTGG 1 cut(s) 2492
VneI GTGCAC 1 cut(s) 2368
VpaK11BI GGWCC 2 cut(s) 99, 791
XapI RAATTY 8 cut(s) 479, 728, 797, 1051, 1424, 1596, 1753, 2320
XbaI TCTAGA 1 cut(s) 1471
XceI RCATGY 4 cut(s) 1113, 1461, 2167, 2368
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmaJI CCTAGG 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 1255, 1842
XmnI GAANNNNTTC 2 cut(s) 1707, 2022
XspI CTAG 9 cut(s) 36, 1158, 1472, 1569, 2112, 2211, 2288, 2331, 2511
ZraI GACGTC 2 cut(s) 42, 865
Zsp2I ATGCAT 1 cut(s) 2080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.