RLG00000012459

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006341
Physical Location & Seq
Reverse (-)
18996407 .. 19001996
5590 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012459

Sequence Viewer

Length: 2856 bp
ATGGGCTACTATCAGAACTTCAAGAAACCCACTAAAGAGAGAAGAAGAAGCCCATCGATTTTAGCAAACGGAGTCCAAGGCTCGGTCTTGCAGAACGGTTGGGTCGGTCAAGTGAAATTGTGGTACCATCCGTTGCAGAATGCAAGGGCAGAAGCCTCAGTTGTGGGAGCTGAAGAAACCCATAGGTCCAATGCTGTTGTGGATACTTCCAAGCAAGGGCCAATCCAGATGGACGCAACATCTGACATGACTGCCTTGGAGAAGGTCAAAACATTTCTTCACAATAGGCCATGGTTGCTTTTCGACCAAAGTGATCACACTTCAGTGCAATCTGACCCACAACAGTTTCATATGGAACCCCCTCCGGAAACTTGCCCCAATGGTGTTACTCGTGGAAGCCCTGAATCCAGTTATTGTGTAAAGGAACCCCGCCTCCCGAAAGGTGTTACTCGTGGATGTCCAGAATCCGGTTATTGTGTAAAGGTAACAGAAAGGTGGCATCCTAAGGGTGCAGTACTAGACGCTCTTGAAAAGGCTCCTGTTTTCTATCCAACACGTGAGGAATTTAAGGACACTCTTAAGTATGTTACAAGTATAAGTGCAAGAGCAGCGCAATATGGTATCTGTCGTATTAGTCCTCCTCCTTCCTGGAAACCTCCATCCCTTATTGAGGAAAACAGCATATGGAATAGTTCCACATTTGCTCCCCATATTCAGCGGATTGATGGGCTGCGGAGTAAAATGGATGGCTCTGATGAGAGCACAAAAAAGAAGAGAAGAATCTCGACAGTGGGTTTGGACTCTGGATTTGAATCTACCTCAAGTCTGGGTGAAACAGGACAGTCTGATGTCAATTGCTTTGAATTGGAACCTGGTCCAGAATTCACTCTTGACAGTTTTAAGAGGTATGCAGATGATTTCAAGCATCAGTATTTCTGCAAAAGTGATGTCATAGGTAATCAAGAGCAGTGGGAGCCATCAGTGGAGAATATTGAAGCTGAATATAAACGTATCATTGAAAACCCAACTGAAGGAATCGAGGTGCTTTGTGGAAATTATTTGGAGACTAAAGCATTTGGCAGTGGATTTCCAACAGTATCACATGATTCCAATCCTTTGGAAACATCAGATTACCCTGATTGCTTGCCATCTGGCTGGAATTTAAATAATCTACCCAGACTCCCTGGTTCTCTTCTTTCCTTTGAAAGCCATGACACATGTCATATTTTAGTGCCTCAGACTCATGTAGGAATGTGCTTTTCATCTAGTCTTTGGAGAGTTGAAGAGCACCACTTATACTCACTGTGTTACAATCATCTGGGTGCTCCTAAAGTTTGGTATGGTGTCCCTGGGAAATCCCGCATAAATTTTGAGGCAGCCATGAGGAAGTCCTTTCCAGATTTATTAAAACAGCATAGGCTGGTTAAGCAAGTATCCCCCTCCACATTGAAATCAGAGGACATACCAGTCTTTCGTTGCATTCAGCGTCCTGGAGAGTTTGTTCTCGTCTTCCCTGGAGCTTATCATTCAGAATTTGATTGTGGCTTTAATTTTTCTGAGAGTACATGTTTTGCTCCTCTAGACTGGTTGCCTCATGGACAGAATGCTGTTGAACTTTACTCTGAACAGGGGAGGAAAACAGCTATATCCTATGATAAGGTGTTGCTTGGAGCAGCTAGGGAAGCTGTAAGGGCACAATGGGAAATTTCACTGTTGAAGAACAAAAACTCAGAGAATTTACGCTGGAAAAATGCCTGCTTAAAGGATGGGATCTTAACAGAAGCACTCAAGTTGCGTCTCAAGTCTGAAGAGATTCGAAGGAAATATTTTTGCACCTCTTTGAAGTCACAGAGGATGAATTCAGATTTTGATGCTACCATGAAAAGGGAATGCAGCATTTGTTTCTGCGATCTTCATTTCTCTGCAGTAGGTTGTCCTTGTTCTGTAGACAGATACTCATGTCTGTTTCATGCAAAGCAGTTCTGTTTTTGTGCTTGGAGTAACAAGTTTTTCCTCTACCGTCATGAAATCAGCGAGTTAAATCTACTTGTTGAAGCTTTGGAAGGAAAATTAAGTGCGGTCATCAAATGGGCGAAAGATGATCTTGGCCTGTATTTGCATGAACATCTTCCCAAAAGCAATGCACAATCACCTGGGCAGGTAGATAATCCCATCTCTCTCACTGATAAAATAAAGCTGAAACAGTACACATTGGAAGATATAACGCCACGGATTTTCAGTTGTGGCACTGATCCCATCTATCTCACTGATAAAAGAAAGCTGAAACAGTACACATTGGAAGATATATCGCCACCGATTTTAGGTTTTGGCTCTACTTCTAGCATAAAGGCGGAATTGAAGGCACGTATGCTGCAATCCACAATTTCAAACATGTTGAAAGCAAATGGTAATCCAATAGCATCCCTTGATGCTGCAACCGGTGCTGCTTCTAGCATCAGGGCTGAATTGAAGGCACGTCTCCTGCAATCTTCAATCTCCAACACATTGAAAGGAAATGATAACCCAACAGGATCACTCAATGCTGCAACAGCCAATGGCAATGGTAGAAATTCTGCTTCTAGCATCGATGTAGAGATGAAGGCACATGAATTCCAATCAACAATCCCAAATGAATATAAAGGCGTGTGCACTCCGCAACAGCCAGTGGCAGCGGCAGAAACTCCTATTCTGCCTATAGAGGTGGCCTCTGATGTTTCATCTGTAACTTCAAGTGAATCATCCTCGGAATCTGATGATATAATACCAGATCTTGGCAAGCTTTCAGAAGCTAGTCATCAAGCAGAAAGTACGGCACCTCAGGACATTCATATTATTAGTGATGACAGTGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

952

Amino Acids

105.87

Weight (kDa)

6.1

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 180 - 213 3.4e-14 jmjN domain
JmjC PF02373 415 - 524 3.2e-32 JmjC domain, hydroxylase
zf-C5HC2 PF02928 631 - 681 4.2e-13 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1466
Acc36I ACCTGC 1 cut(s) 2149
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 2 cut(s) 123, 2812
AccB7I CCANNNNNTGG 1 cut(s) 2771
AccI GTMKAC 1 cut(s) 1947
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 8 cut(s) 430, 718, 733, 1360, 2078, 2351, 2654, 2672
AclWI GGATC 3 cut(s) 1778, 2246, 2539
AcuI CTGAAG 4 cut(s) 192, 306, 1050, 1827
AcvI CACGTG 1 cut(s) 557
AfaI GTAC 6 cut(s) 125, 516, 1564, 2207, 2291, 2809
AfiI CCNNNNNNNGG 8 cut(s) 82, 216, 467, 670, 1031, 1884, 2321, 2771
AflII CTTAAG 1 cut(s) 578
AflIII ACRYGT 4 cut(s) 554, 1217, 1565, 2391
AgeI ACCGGT 1 cut(s) 2438
AjiI CACGTC 1 cut(s) 2477
AjnI CCWGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
AleI CACNNNNGTG 1 cut(s) 323
AloI GAACNNNNNNTCC 4 cut(s) 417, 449, 1485, 1517
Alw21I GWGCWC 4 cut(s) 764, 1290, 1327, 2651
Alw26I GTCTC 3 cut(s) 1058, 1802, 2483
Alw44I GTGCAC 1 cut(s) 2647
AlwI GGATC 3 cut(s) 1778, 2246, 2539
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 4 cut(s) 218, 287, 2107, 2703
ApaLI GTGCAC 1 cut(s) 2647
ArsI GACNNNNNNTTYG 6 cut(s) 778, 791, 810, 823, 933, 965
AsiGI ACCGGT 1 cut(s) 2438
Asp700I GAANNNNTTC 2 cut(s) 1812, 2127
Asp718I GGTACC 1 cut(s) 123
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 3 cut(s) 186, 218, 875
AsuHPI GGTGA 2 cut(s) 842, 2142
AsuII TTCGAA 1 cut(s) 1816
AvaII GGWCC 2 cut(s) 186, 875
AxyI CCTNAGG 2 cut(s) 504, 2817
BaeGI GKGCMC 2 cut(s) 1696, 2651
BaeI ACNNNNGTAYC 2 cut(s) 1945, 1978
BanI GGYRCC 2 cut(s) 123, 2812
BauI CACGAG 2 cut(s) 390, 450
BbrPI CACGTG 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 1501
Bbv12I GWGCWC 4 cut(s) 764, 1290, 1327, 2651
BceAI ACGGC 1 cut(s) 2826
BciT130I CCWGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
BciVI GTATCC 2 cut(s) 196, 1444
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 3 cut(s) 1058, 1802, 2483
BfaI CTAG 8 cut(s) 518, 1266, 1580, 1677, 2340, 2451, 2580, 2790
BfmI CTRYAG 3 cut(s) 1923, 1944, 2694
BfrI CTTAAG 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 2149
BfuI GTATCC 2 cut(s) 196, 1444
BglII AGATCT 1 cut(s) 2767
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
Bme18I GGWCC 2 cut(s) 186, 875
BmgBI CACGTC 1 cut(s) 2477
BmgT120I GGNCC 3 cut(s) 186, 218, 875
BmiI GGNNCC 7 cut(s) 125, 357, 426, 537, 870, 975, 2814
BmrFI CCNGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
BmsI GCATC 7 cut(s) 508, 934, 1861, 2419, 2429, 2463, 2592
BoxI GACNNNNGTC 1 cut(s) 1218
BpiI GAAGAC 1 cut(s) 1501
BplI GAGNNNNNCTC 2 cut(s) 2690, 2722
BpmI CTGGAG 2 cut(s) 1512, 1536
Bpu14I TTCGAA 1 cut(s) 1816
BpuEI CTTGAG 3 cut(s) 805, 1772, 1784
Bsa29I ATCGAT 2 cut(s) 56, 2586
BsaAI YACGTR 2 cut(s) 557, 2366
BsaBI GATNNNNATC 2 cut(s) 811, 1110
BsaWI WCCGGW 3 cut(s) 364, 467, 2438
BsaXI ACNNNNNCTCC 8 cut(s) 417, 447, 688, 718, 1164, 1194, 1485, 1515
Bsc4I CCNNNNNNNGG 8 cut(s) 82, 216, 467, 670, 1031, 1884, 2321, 2771
Bse118I RCCGGY 1 cut(s) 2438
Bse1I ACTGG 4 cut(s) 408, 1466, 1589, 2663
Bse21I CCTNAGG 2 cut(s) 504, 2817
Bse3DI GCAATG 2 cut(s) 2146, 2566
Bse8I GATNNNNATC 2 cut(s) 811, 1110
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
BseCI ATCGAT 2 cut(s) 56, 2586
BseGI GGATG 9 cut(s) 127, 461, 499, 659, 751, 1771, 1860, 2420, 2738
BseJI GATNNNNATC 2 cut(s) 811, 1110
BseLI CCNNNNNNNGG 8 cut(s) 82, 216, 467, 670, 1031, 1884, 2321, 2771
BseMI GCAATG 2 cut(s) 2146, 2566
BseMII CTCAG 5 cut(s) 171, 1250, 1548, 1743, 2831
BseNI ACTGG 4 cut(s) 408, 1466, 1589, 2663
BseRI GAGGAG 2 cut(s) 630, 1566
BseSI GKGCMC 2 cut(s) 1696, 2651
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 4 cut(s) 220, 289, 2109, 2705
BshNI GGYRCC 2 cut(s) 123, 2812
BshTI ACCGGT 1 cut(s) 2438
BshVI ATCGAT 2 cut(s) 56, 2586
BsiHKAI GWGCWC 4 cut(s) 764, 1290, 1327, 2651
BsiSI CCGG 3 cut(s) 365, 468, 2439
BslFI GGGAC 1 cut(s) 1331
BslI CCNNNNNNNGG 8 cut(s) 82, 216, 467, 670, 1031, 1884, 2321, 2771
BsmAI GTCTC 3 cut(s) 1058, 1802, 2483
BsmBI CGTCTC 2 cut(s) 1802, 2483
BsmFI GGGAC 1 cut(s) 1331
BsmI GAATGC 4 cut(s) 145, 1479, 1609, 1895
BsnI GGCC 4 cut(s) 220, 289, 2109, 2705
Bsp119I TTCGAA 1 cut(s) 1816
Bsp1286I GDGCHC 5 cut(s) 764, 1290, 1327, 1696, 2651
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
Bsp19I CCATGG 1 cut(s) 290
BspACI CCGC 8 cut(s) 430, 718, 733, 1360, 2078, 2351, 2654, 2672
BspANI GGCC 4 cut(s) 220, 289, 2109, 2705
BspCNI CTCAG 5 cut(s) 170, 1249, 1549, 1742, 2830
BspDI ATCGAT 2 cut(s) 56, 2586
BspEI TCCGGA 1 cut(s) 364
BspHI TCATGA 1 cut(s) 2023
BspLI GGNNCC 7 cut(s) 125, 357, 426, 537, 870, 975, 2814
BspMAI CTGCAG 1 cut(s) 1927
BspMI ACCTGC 1 cut(s) 2149
BspPI GGATC 3 cut(s) 1778, 2246, 2539
BspQI GCTCTTC 1 cut(s) 1278
BspT104I TTCGAA 1 cut(s) 1816
BspT107I GGYRCC 2 cut(s) 123, 2812
BspTI CTTAAG 1 cut(s) 578
BsrDI GCAATG 2 cut(s) 2146, 2566
BsrFI RCCGGY 1 cut(s) 2438
BsrI ACTGG 4 cut(s) 408, 1466, 1589, 2663
BssAI RCCGGY 1 cut(s) 2438
BssMI GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
BssSI CACGAG 2 cut(s) 390, 450
BssT1I CCWWGG 3 cut(s) 76, 255, 290
Bst2BI CACGAG 2 cut(s) 390, 450
Bst2UI CCWGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
Bst6I CTCTTC 4 cut(s) 767, 1197, 1278, 1803
BstAFI CTTAAG 1 cut(s) 578
BstAPI GCANNNNNTGC 1 cut(s) 2441
BstBAI YACGTR 2 cut(s) 557, 2366
BstBI TTCGAA 1 cut(s) 1816
BstC8I GCNNGC 3 cut(s) 1145, 1756, 2777
BstDEI CTNAG 6 cut(s) 157, 504, 1236, 1557, 1729, 2817
BstDSI CCRYGG 2 cut(s) 290, 2228
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 9 cut(s) 127, 461, 499, 659, 751, 1771, 1860, 2420, 2738
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 7 cut(s) 316, 1773, 1912, 2104, 2254, 2534, 2770
BstMAI GTCTC 3 cut(s) 1058, 1802, 2483
BstMBI GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
BstMWI GCNNNNNNNGC 8 cut(s) 295, 608, 973, 1426, 1682, 1691, 2441, 2549
BstNI CCWGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
BstNSI RCATGY 3 cut(s) 1221, 1569, 2395
BstPAI GACNNNNGTC 1 cut(s) 1218
BstSCI CCNGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
BstSFI CTRYAG 3 cut(s) 1923, 1944, 2694
BstSLI GKGCMC 2 cut(s) 1696, 2651
BstV2I GAAGAC 1 cut(s) 1501
BstX2I RGATCY 2 cut(s) 1770, 2767
BstXI CCANNNNNNTGG 2 cut(s) 1117, 1155
BstYI RGATCY 2 cut(s) 1770, 2767
Bsu15I ATCGAT 2 cut(s) 56, 2586
Bsu36I CCTNAGG 2 cut(s) 504, 2817
BsuI GTATCC 2 cut(s) 196, 1444
BsuRI GGCC 4 cut(s) 220, 289, 2109, 2705
BsuTUI ATCGAT 2 cut(s) 56, 2586
BtgI CCRYGG 2 cut(s) 290, 2228
BtrI CACGTC 1 cut(s) 2477
BtsCI GGATG 9 cut(s) 127, 461, 499, 659, 751, 1771, 1860, 2420, 2738
BtsI GCAGTG 2 cut(s) 974, 1087
BveI ACCTGC 1 cut(s) 2149
Cac8I GCNNGC 3 cut(s) 1145, 1756, 2777
CciI TCATGA 1 cut(s) 2023
CfoI GCGC 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 2438
Cfr13I GGNCC 3 cut(s) 186, 218, 875
ClaI ATCGAT 2 cut(s) 56, 2586
CseI GACGC 4 cut(s) 242, 530, 1475, 1784
CsiI ACCWGGT 1 cut(s) 871
Csp6I GTAC 6 cut(s) 124, 515, 1563, 2206, 2290, 2808
CspAI ACCGGT 1 cut(s) 2438
CspCI CAANNNNNGTGG 2 cut(s) 950, 985
CviQI GTAC 6 cut(s) 124, 515, 1563, 2206, 2290, 2808
DdeI CTNAG 6 cut(s) 157, 504, 1236, 1557, 1729, 2817
DpnI GATC 7 cut(s) 315, 1772, 1911, 2103, 2253, 2533, 2769
DpnII GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
DraI TTTAAA 1 cut(s) 1164
DrdI GACNNNNNNGTC 1 cut(s) 1466
DseDI GACNNNNNNGTC 1 cut(s) 1466
Eam1104I CTCTTC 4 cut(s) 767, 1197, 1278, 1803
EarI CTCTTC 4 cut(s) 767, 1197, 1278, 1803
EciI GGCGGA 1 cut(s) 2366
Eco130I CCWWGG 3 cut(s) 76, 255, 290
Eco47I GGWCC 2 cut(s) 186, 875
Eco57I CTGAAG 4 cut(s) 192, 306, 1050, 1827
Eco72I CACGTG 1 cut(s) 557
Eco81I CCTNAGG 2 cut(s) 504, 2817
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoRI GAATTC 3 cut(s) 881, 1858, 2609
EcoRII CCWGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
EcoT14I CCWWGG 3 cut(s) 76, 255, 290
ErhI CCWWGG 3 cut(s) 76, 255, 290
Esp3I CGTCTC 2 cut(s) 1802, 2483
FalI AAGNNNNNCTT 2 cut(s) 2088, 2120
FaqI GGGAC 1 cut(s) 1331
FauI CCCGC 2 cut(s) 437, 1367
FauNDI CATATG 2 cut(s) 351, 683
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 1947
FokI GGATG 9 cut(s) 114, 468, 486, 646, 758, 1778, 1867, 2407, 2725
FspBI CTAG 8 cut(s) 518, 1266, 1580, 1677, 2340, 2451, 2580, 2790
GlaI GCGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 1512, 1536
HaeIII GGCC 4 cut(s) 220, 289, 2109, 2705
HapII CCGG 3 cut(s) 365, 468, 2439
HgaI GACGC 4 cut(s) 242, 530, 1475, 1784
HhaI GCGC 1 cut(s) 613
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HindIII AAGCTT 2 cut(s) 2055, 2777
HpaII CCGG 3 cut(s) 365, 468, 2439
HphI GGTGA 2 cut(s) 842, 2142
Hpy166II GTNNAC 4 cut(s) 1948, 2208, 2292, 2649
Hpy8I GTNNAC 4 cut(s) 1948, 2208, 2292, 2649
HpyAV CCTTC 8 cut(s) 256, 654, 1025, 1812, 2057, 2353, 2464, 2593
HpyCH4IV ACGT 4 cut(s) 556, 1009, 2365, 2476
HpyF10VI GCNNNNNNNGC 8 cut(s) 295, 608, 973, 1426, 1682, 1691, 2441, 2549
HpyF3I CTNAG 6 cut(s) 157, 504, 1236, 1557, 1729, 2817
HpySE526I ACGT 4 cut(s) 556, 1009, 2365, 2476
HspAI GCGC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 364
KpnI GGTACC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
LguI GCTCTTC 1 cut(s) 1278
LmnI GCTCC 8 cut(s) 167, 541, 709, 973, 1330, 1517, 1579, 1670
LweI GCATC 7 cut(s) 508, 934, 1861, 2419, 2429, 2463, 2592
MabI ACCWGGT 1 cut(s) 871
MaeI CTAG 8 cut(s) 518, 1266, 1580, 1677, 2340, 2451, 2580, 2790
MaeII ACGT 4 cut(s) 556, 1009, 2365, 2476
MaeIII GTNAC 8 cut(s) 385, 445, 484, 586, 1307, 1845, 2000, 2722
MalI GATC 7 cut(s) 315, 1772, 1911, 2103, 2253, 2533, 2769
MboI GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
MfeI CAATTG 1 cut(s) 853
MflI RGATCY 2 cut(s) 1770, 2767
MhlI GDGCHC 5 cut(s) 764, 1290, 1327, 1696, 2651
MlyI GAGTC 4 cut(s) 81, 794, 1173, 1234
MmeI TCCRAC 3 cut(s) 575, 1115, 2523
MroI TCCGGA 1 cut(s) 364
MroXI GAANNNNTTC 2 cut(s) 1812, 2127
MslI CAYNNNNRTG 2 cut(s) 323, 1320
MspA1I CMGCKG 2 cut(s) 718, 2672
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 3 cut(s) 365, 468, 2439
MspR9I CCNGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
MunI CAATTG 1 cut(s) 853
Mva1269I GAATGC 4 cut(s) 145, 1479, 1609, 1895
MvaI CCWGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
MwoI GCNNNNNNNGC 8 cut(s) 295, 608, 973, 1426, 1682, 1691, 2441, 2549
NcoI CCATGG 1 cut(s) 290
NdeI CATATG 2 cut(s) 351, 683
NdeII GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
NlaIV GGNNCC 7 cut(s) 125, 357, 426, 537, 870, 975, 2814
NmuCI GTSAC 1 cut(s) 1845
NspI RCATGY 3 cut(s) 1221, 1569, 2395
NspV TTCGAA 1 cut(s) 1816
OliI CACNNNNGTG 1 cut(s) 323
PagI TCATGA 1 cut(s) 2023
PasI CCCWGGG 1 cut(s) 1349
PciI ACATGT 3 cut(s) 1217, 1565, 2391
PciSI GCTCTTC 1 cut(s) 1278
PctI GAATGC 4 cut(s) 145, 1479, 1609, 1895
PdmI GAANNNNTTC 2 cut(s) 1812, 2127
PfeI GAWTC 9 cut(s) 404, 464, 780, 812, 1035, 1106, 1813, 2735, 2747
PflMI CCANNNNNTGG 1 cut(s) 2771
PfoI TCCNGGA 2 cut(s) 647, 1489
PinAI ACCGGT 1 cut(s) 2438
PleI GAGTC 4 cut(s) 80, 794, 1173, 1234
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
PpsI GAGTC 4 cut(s) 80, 794, 1173, 1234
Ppu21I YACGTR 2 cut(s) 557, 2366
PscI ACATGT 3 cut(s) 1217, 1565, 2391
PshAI GACNNNNGTC 1 cut(s) 1218
Psp6I CCWGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
PspCI CACGTG 1 cut(s) 557
PspGI CCWGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
PspN4I GGNNCC 7 cut(s) 125, 357, 426, 537, 870, 975, 2814
PspPI GGNCC 3 cut(s) 186, 218, 875
PstI CTGCAG 1 cut(s) 1927
PsuI RGATCY 2 cut(s) 1770, 2767
RsaI GTAC 6 cut(s) 125, 516, 1564, 2207, 2291, 2809
RsaNI GTAC 6 cut(s) 124, 515, 1563, 2206, 2290, 2808
RseI CAYNNNNRTG 2 cut(s) 323, 1320
SapI GCTCTTC 1 cut(s) 1278
Sau3AI GATC 7 cut(s) 313, 1770, 1909, 2101, 2251, 2531, 2767
Sau96I GGNCC 3 cut(s) 186, 218, 875
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 4 cut(s) 81, 794, 1173, 1234
ScrFI CCNGG 7 cut(s) 649, 873, 1185, 1350, 1491, 1515, 2154
SduI GDGCHC 5 cut(s) 764, 1290, 1327, 1696, 2651
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 7 cut(s) 508, 934, 1861, 2419, 2429, 2463, 2592
SfcI CTRYAG 3 cut(s) 1923, 1944, 2694
SfuI TTCGAA 1 cut(s) 1816
SinI GGWCC 2 cut(s) 186, 875
SmiI ATTTAAAT 1 cut(s) 1164
SmiMI CAYNNNNRTG 2 cut(s) 323, 1320
SmlI CTYRAG 4 cut(s) 578, 820, 1787, 1799
SmoI CTYRAG 4 cut(s) 578, 820, 1787, 1799
SsiI CCGC 8 cut(s) 430, 718, 733, 1360, 2078, 2351, 2654, 2672
SspI AATATT 2 cut(s) 991, 1826
SspMI CTAG 8 cut(s) 518, 1266, 1580, 1677, 2340, 2451, 2580, 2790
StyD4I CCNGG 7 cut(s) 647, 871, 1183, 1348, 1489, 1513, 2152
StyI CCWWGG 3 cut(s) 76, 255, 290
SwaI ATTTAAAT 1 cut(s) 1164
TaiI ACGT 4 cut(s) 559, 1012, 2368, 2479
TaqI TCGA 6 cut(s) 56, 303, 785, 1038, 1816, 2586
TaqII GACCGA 2 cut(s) 73, 95
TatI WGTACW 4 cut(s) 514, 1562, 2205, 2289
TauI GCSGC 1 cut(s) 2675
TfiI GAWTC 9 cut(s) 404, 464, 780, 812, 1035, 1106, 1813, 2735, 2747
TseFI GTSAC 1 cut(s) 1845
Tsp45I GTSAC 1 cut(s) 1845
TspGWI ACGGA 3 cut(s) 84, 120, 2245
Van91I CCANNNNNTGG 1 cut(s) 2771
Vha464I CTTAAG 1 cut(s) 578
VneI GTGCAC 1 cut(s) 2647
VpaK11BI GGWCC 2 cut(s) 186, 875
XagI CCTNNNNNAGG 1 cut(s) 668
XbaI TCTAGA 1 cut(s) 1579
XceI RCATGY 3 cut(s) 1221, 1569, 2395
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 1947
XmnI GAANNNNTTC 2 cut(s) 1812, 2127
XspI CTAG 8 cut(s) 518, 1266, 1580, 1677, 2340, 2451, 2580, 2790
ZrmI AGTACT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.