Rw6G026120

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006341
Physical Location & Seq
Forward (+)
49516473 .. 49521070
4598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026120.1

Sequence Viewer

Length: 2703 bp
ATGAAAAACAATTCGGTTCCACCTGGTTTTGCGCCTCGTACTTCCTTCACGCTGAAGAGGGCAGAAGTCTCAGTTGTGGGAGCTGAAGAAACCCATAGGTCCAATGATGTTGTGGATACTTCCAAGCAGGGGCCAATCCAGATGGATGCAACATCTGACATGACTGCCTTGGAGAAGGTCAAAACATTTCTTCACAATAGGCCATGGTTGCTTTTCGACCAAAGTGATCACACTTCAGTGCAATCTGACCCACGACAGTTTCATATGGAACCCCGCCTCCCGAAAGGTGTTACTCGTGGATGTCCAGAGTCCGGTTATTGTGTAAAGGTGACAGAAAGGTGGCATCCTGAGGGTGCAGTAATAGACACTCTTGAAAAGGCTCCTGTTTTCTATCCAACACATGAGGAATTTAAGGACACTCTTAAGTATGTTACAAGTATAAGTGCAAGAGCAGCGCAATATGGTATCTGTCGTATTAGTCCTCCTCCTTCCTGGAAACCCCCATCCCTTCTTGAGGAAAACAGCATATGGAATAGTTCCACATTTGCTCCCCATATTCAGCGGATTGATGGGCTGGGGAGTAAAATGGATGGCTCTGATGAGAGCACAAAAAAGAAGAGAAGAATATCGACAGTGGGTTTGGACTCAGGATTTGAATCTACCTCAAGTCTGGGTGAAACAGGACAGTCTGATGTCAATTGCTTTGAATCGGAACCTGGTCCAGAATTCACTCTTGACAGTTTTAAGAGGTATGCAGATGATTTCAAGCATCAGTATTTCTGCAAAAGTGACGTAATAGGTAATCAAGAGCAGTGGGAGCCGTCAGTGGAGAATATTGAAGCTGAATATAAACGTATCATTGAAAACCCAACTGAAGGAATCGAGGTGCTTTGTGGAAATTATTTGGAGACTAAAGCGTTTGGCAGTGGATTTCCAACAGTATCACATGATTCCAATCCTTTGGAAACATCAGATTACCCTGATTGCTTGCCATCTGGCTGGAATTTAAATAATCTACCCAGACTCCCTGGTTCTCTTCTTTCTTTTGAAAGCCATGACACATGTCATATTTTAGTGCCTCAGACTCGTGTAGGAATGTGTTTTTCATCTAGTCTTTGGAGAGTTGAAGAGCACCACTTATACTCATTGTCTTACAATCATCTGGGTGCTCCTAAAGTTTGGTATGGTGTCCCTGGGAAATCCCGCATAAATTTTGAGGCAGCCATGAGGAAGTCCTTTCCAGATTTATTAAAACAGCATAGGCTGGTTAAGCAAGTATCCCCCTCCACGTTGAAATCAGAGGACATACCTGTCTTTCGTTGCATTCAGCGTCCTGGAGAGTTTGTTCTTGTCTTCCCTGGAGCTTATCATTCAGAATTTGATTGTGGCTTTAATTTTTCTGAGAGGGCATGTTTTGCTCCTCTAGACTGGTTGCCTCATGGACAGAATGCTGTTGAACTTTACTCTGAACAGGGGAGGAAAACAGCTATATCCTATGATAAGGTGTTGCTTGGAGCAGCTAGGGAAGCTGTAAGGGCACAATGGGAAATTTCACTGTTGAAGAAGAACAAAAACTCAGAGAATTTACGCTGGAAAAATGCCTGCTTAAAGGATGGGATCTTAACAGAAGCACTCAAGTTGCGTCTCAAGTCTGAAGAGATTCGAAGGAAATATTTTTGCACCTCTTTGAAGTCACAGAGGATGAATTCAGATTTTGATGCTACCATGAAAAGGGAATGCAGCATTTGTTTCTGCGATCTTCATTTCTCTGCAGTAGGTTGTCCTTGTTCTGTAGACAGATACTCATGTCTGTTTCATGCAAAGCAGTTCTGTTTTTGTGCTTGGAGTAACAAGTTTTTCCTCTACCGTCATGAAATCAGCGAGCTAAATCTACTTGTTGAAGCTTTGGAAGGAAAATTAAGTGCAGTCATCAAATGGGCGAAAGATGATCTTGGCCTGTATTTGCATGAACATCTTCCCAAAAGCAATGCACAATCACCTGGGCAGGTAGATAATCCCATCTCTCCCACTGATAAAATAAAGCCGAAACAGTACACATCGGAAGACATAACGCCACCGATTTTCAGTTGTGGCACTGGTCCCATTTATCTCACTGATAAAAGAAAGCTGAAACAGTACACATTGGAAGATATATCGCCACCGATTTTGGGTTTTGGCTCTGCTTCTAGCATAAAGGCGGAATTGAAGGCACGTATGCTGCAATCCACAATTTCAAACATGTTGAAAGCTAACGGTAATCCAATAGCATCCCTTGATGCTGCAACCAGTGCTGCTTCTAGCATCAGGGCTGAATTGAAGGCACGTCTCCTGCAATCTTCAATCTCCAACACATTGAAAGGAAATGATAATCCAGCAGGATCACTCAATGCTGCGACAGCCAATGGCAATGGTAGAAATTCTGCTTCTAGCATCGAGGTAGAGATGAAGGCACATGAATTCCAATCAACAATCCCAAATGAATATAAAGGTGTGTGCACTCCGCAACAGCCAGTGGCAGCGGTAGAAACTCCTATTCTGCCTATAGAGGTGGCCTCTGATGTTTCATCTGTAACTTCAAGTGAATCATCCTCGGAATCTGATGATATAATACCAGATCTTGGCAAGCTTTCAGAAGCTAATCATCAAGCAGAAAGTACGGCACCTCAGAACATTCATATTCTTAGTGATGACAGTGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

900

Amino Acids

100.02

Weight (kDa)

5.97

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 128 - 161 3.8e-14 jmjN domain
JmjC PF02373 364 - 470 2.6e-31 JmjC domain, hydroxylase
zf-C5HC2 PF02928 580 - 630 3.9e-13 C5HC2 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1310
Acc36I ACCTGC 1 cut(s) 1996
AccB1I GGYRCC 1 cut(s) 2659
AccB7I CCANNNNNTGG 1 cut(s) 2618
AccI GTMKAC 1 cut(s) 1794
AciI CCGC 6 cut(s) 274, 562, 1204, 2198, 2501, 2519
AclWI GGATC 2 cut(s) 1625, 2386
AcuI CTGAAG 5 cut(s) 74, 105, 219, 894, 1674
AfaI GTAC 4 cut(s) 40, 2054, 2138, 2656
AfiI CCNNNNNNNGG 7 cut(s) 129, 311, 514, 875, 1731, 2168, 2618
AflII CTTAAG 1 cut(s) 422
AflIII ACRYGT 2 cut(s) 1061, 2238
AjiI CACGTC 1 cut(s) 2324
AjnI CCWGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
AleI CACNNNNGTG 1 cut(s) 236
AloI GAACNNNNNNTCC 4 cut(s) 261, 293, 1329, 1361
Alw21I GWGCWC 4 cut(s) 608, 1134, 1171, 2498
Alw26I GTCTC 4 cut(s) 73, 902, 1649, 2330
Alw44I GTGCAC 1 cut(s) 2494
AlwI GGATC 2 cut(s) 1625, 2386
AoxI GGCC 4 cut(s) 131, 200, 1954, 2550
ApaLI GTGCAC 1 cut(s) 2494
ApeKI GCWGC 9 cut(s) 452, 1220, 1517, 1740, 2218, 2279, 2291, 2390, 2516
ArsI GACNNNNNNTTYG 4 cut(s) 622, 635, 654, 667
Asp700I GAANNNNTTC 2 cut(s) 1659, 1974
AspLEI GCGC 2 cut(s) 34, 457
AspS9I GGNCC 4 cut(s) 99, 131, 719, 2099
AsuHPI GGTGA 3 cut(s) 340, 686, 1989
AsuII TTCGAA 1 cut(s) 1663
AvaII GGWCC 3 cut(s) 99, 719, 2099
AxyI CCTNAGG 1 cut(s) 348
BaeGI GKGCMC 2 cut(s) 1540, 2498
BaeI ACNNNNGTAYC 2 cut(s) 1792, 1825
BanI GGYRCC 1 cut(s) 2659
BauI CACGAG 2 cut(s) 294, 1086
BbsI GAAGAC 2 cut(s) 1345, 2070
Bbv12I GWGCWC 4 cut(s) 608, 1134, 1171, 2498
BbvI GCAGC 9 cut(s) 464, 1232, 1529, 1752, 2205, 2266, 2278, 2377, 2528
BccI CCATC 8 cut(s) 136, 511, 563, 584, 1000, 1607, 2027, 2690
BceAI ACGGC 2 cut(s) 805, 2673
BciT130I CCWGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
BciVI GTATCC 2 cut(s) 109, 1288
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 4 cut(s) 73, 902, 1649, 2330
BfaI CTAG 6 cut(s) 1110, 1424, 1521, 2187, 2298, 2427
BfmI CTRYAG 3 cut(s) 1770, 1791, 2541
BfrI CTTAAG 1 cut(s) 422
BfuAI ACCTGC 1 cut(s) 1996
BfuI GTATCC 2 cut(s) 109, 1288
BglII AGATCT 1 cut(s) 2614
BisI GCNGC 9 cut(s) 453, 1221, 1518, 1741, 2219, 2280, 2292, 2391, 2517
BlsI GCNGC 9 cut(s) 454, 1222, 1519, 1742, 2220, 2281, 2293, 2392, 2518
Bme1390I CCNGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
Bme18I GGWCC 3 cut(s) 99, 719, 2099
BmgBI CACGTC 1 cut(s) 2324
BmgT120I GGNCC 4 cut(s) 99, 131, 719, 2099
BmiI GGNNCC 8 cut(s) 18, 132, 270, 381, 714, 819, 2101, 2661
BmrFI CCNGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
BmsI GCATC 8 cut(s) 136, 352, 778, 1708, 2266, 2276, 2310, 2439
BoxI GACNNNNGTC 1 cut(s) 1062
BpiI GAAGAC 2 cut(s) 1345, 2070
BplI GAGNNNNNCTC 2 cut(s) 2537, 2569
BpmI CTGGAG 2 cut(s) 1356, 1380
Bpu14I TTCGAA 1 cut(s) 1663
BpuEI CTTGAG 4 cut(s) 533, 649, 1619, 1631
BsaAI YACGTR 1 cut(s) 2213
BsaBI GATNNNNATC 2 cut(s) 655, 954
BsaJI CCNNGG 8 cut(s) 168, 203, 1027, 1192, 1193, 1357, 2000, 2589
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 8 cut(s) 261, 291, 532, 562, 1008, 1038, 1329, 1359
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 311, 514, 875, 1731, 2168, 2618
Bse1I ACTGG 4 cut(s) 1433, 2101, 2286, 2510
Bse21I CCTNAGG 1 cut(s) 348
Bse3DI GCAATG 2 cut(s) 1993, 2413
Bse8I GATNNNNATC 2 cut(s) 655, 954
BseBI CCWGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
BseDI CCNNGG 8 cut(s) 168, 203, 1027, 1192, 1193, 1357, 2000, 2589
BseGI GGATG 9 cut(s) 151, 305, 343, 503, 595, 1618, 1707, 2267, 2585
BseJI GATNNNNATC 2 cut(s) 655, 954
BseLI CCNNNNNNNGG 7 cut(s) 129, 311, 514, 875, 1731, 2168, 2618
BseMI GCAATG 2 cut(s) 1993, 2413
BseMII CTCAG 7 cut(s) 84, 339, 660, 1094, 1392, 1590, 2678
BseNI ACTGG 4 cut(s) 1433, 2101, 2286, 2510
BseRI GAGGAG 2 cut(s) 474, 1410
BseSI GKGCMC 2 cut(s) 1540, 2498
BseXI GCAGC 9 cut(s) 464, 1232, 1529, 1752, 2205, 2266, 2278, 2377, 2528
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 2 cut(s) 375, 1944
BshFI GGCC 4 cut(s) 133, 202, 1956, 2552
BshNI GGYRCC 1 cut(s) 2659
BsiHKAI GWGCWC 4 cut(s) 608, 1134, 1171, 2498
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 2 cut(s) 1175, 2085
BslI CCNNNNNNNGG 7 cut(s) 129, 311, 514, 875, 1731, 2168, 2618
BsmAI GTCTC 4 cut(s) 73, 902, 1649, 2330
BsmBI CGTCTC 2 cut(s) 1649, 2330
BsmFI GGGAC 2 cut(s) 1175, 2085
BsmI GAATGC 3 cut(s) 1323, 1453, 1742
BsnI GGCC 4 cut(s) 133, 202, 1956, 2552
Bsp119I TTCGAA 1 cut(s) 1663
Bsp1286I GDGCHC 5 cut(s) 608, 1134, 1171, 1540, 2498
Bsp143I GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
Bsp19I CCATGG 1 cut(s) 203
BspACI CCGC 6 cut(s) 274, 562, 1204, 2198, 2501, 2519
BspANI GGCC 4 cut(s) 133, 202, 1956, 2552
BspCNI CTCAG 7 cut(s) 83, 340, 659, 1093, 1393, 1589, 2677
BspHI TCATGA 1 cut(s) 1870
BspLI GGNNCC 8 cut(s) 18, 132, 270, 381, 714, 819, 2101, 2661
BspMAI CTGCAG 1 cut(s) 1774
BspMI ACCTGC 1 cut(s) 1996
BspPI GGATC 2 cut(s) 1625, 2386
BspQI GCTCTTC 1 cut(s) 1122
BspT104I TTCGAA 1 cut(s) 1663
BspT107I GGYRCC 1 cut(s) 2659
BspTI CTTAAG 1 cut(s) 422
BsrDI GCAATG 2 cut(s) 1993, 2413
BsrI ACTGG 4 cut(s) 1433, 2101, 2286, 2510
BssECI CCNNGG 8 cut(s) 168, 203, 1027, 1192, 1193, 1357, 2000, 2589
BssMI GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
BssSI CACGAG 2 cut(s) 294, 1086
BssT1I CCWWGG 2 cut(s) 168, 203
Bst2BI CACGAG 2 cut(s) 294, 1086
Bst2UI CCWGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
Bst6I CTCTTC 5 cut(s) 50, 611, 1041, 1122, 1650
BstAFI CTTAAG 1 cut(s) 422
BstAPI GCANNNNNTGC 2 cut(s) 1415, 2288
BstBAI YACGTR 1 cut(s) 2213
BstBI TTCGAA 1 cut(s) 1663
BstC8I GCNNGC 4 cut(s) 989, 1603, 1883, 2624
BstDEI CTNAG 8 cut(s) 70, 348, 646, 1080, 1401, 1576, 2664, 2681
BstDSI CCRYGG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 9 cut(s) 151, 305, 343, 503, 595, 1618, 1707, 2267, 2585
BstHHI GCGC 2 cut(s) 34, 457
BstKTI GATC 6 cut(s) 229, 1620, 1759, 1951, 2381, 2617
BstMAI GTCTC 4 cut(s) 73, 902, 1649, 2330
BstMBI GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
BstMWI GCNNNNNNNGC 9 cut(s) 208, 452, 817, 1270, 1415, 1526, 1535, 2288, 2396
BstNI CCWGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
BstNSI RCATGY 3 cut(s) 1065, 1413, 2242
BstPAI GACNNNNGTC 1 cut(s) 1062
BstSCI CCNGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
BstSFI CTRYAG 3 cut(s) 1770, 1791, 2541
BstSLI GKGCMC 2 cut(s) 1540, 2498
BstV1I GCAGC 9 cut(s) 464, 1232, 1529, 1752, 2205, 2266, 2278, 2377, 2528
BstV2I GAAGAC 2 cut(s) 1345, 2070
BstX2I RGATCY 2 cut(s) 1617, 2614
BstXI CCANNNNNNTGG 2 cut(s) 961, 999
BstYI RGATCY 2 cut(s) 1617, 2614
Bsu36I CCTNAGG 1 cut(s) 348
BsuI GTATCC 2 cut(s) 109, 1288
BsuRI GGCC 4 cut(s) 133, 202, 1956, 2552
BtgI CCRYGG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 2324
BtsCI GGATG 9 cut(s) 151, 305, 343, 503, 595, 1618, 1707, 2267, 2585
BtsI GCAGTG 2 cut(s) 818, 931
BveI ACCTGC 1 cut(s) 1996
Cac8I GCNNGC 4 cut(s) 989, 1603, 1883, 2624
CciI TCATGA 1 cut(s) 1870
CfoI GCGC 2 cut(s) 34, 457
Cfr13I GGNCC 4 cut(s) 99, 131, 719, 2099
CseI GACGC 2 cut(s) 1319, 1631
CsiI ACCWGGT 2 cut(s) 22, 715
Csp6I GTAC 4 cut(s) 39, 2053, 2137, 2655
CspCI CAANNNNNGTGG 4 cut(s) 794, 829, 2148, 2183
CviQI GTAC 4 cut(s) 39, 2053, 2137, 2655
DdeI CTNAG 8 cut(s) 70, 348, 646, 1080, 1401, 1576, 2664, 2681
DpnI GATC 6 cut(s) 228, 1619, 1758, 1950, 2380, 2616
DpnII GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
DraI TTTAAA 1 cut(s) 1008
DrdI GACNNNNNNGTC 1 cut(s) 1310
DseDI GACNNNNNNGTC 1 cut(s) 1310
Eam1104I CTCTTC 5 cut(s) 50, 611, 1041, 1122, 1650
EarI CTCTTC 5 cut(s) 50, 611, 1041, 1122, 1650
EciI GGCGGA 1 cut(s) 2213
Eco130I CCWWGG 2 cut(s) 168, 203
Eco47I GGWCC 3 cut(s) 99, 719, 2099
Eco57I CTGAAG 5 cut(s) 74, 105, 219, 894, 1674
Eco81I CCTNAGG 1 cut(s) 348
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoRI GAATTC 3 cut(s) 725, 1705, 2456
EcoRII CCWGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
EcoT14I CCWWGG 2 cut(s) 168, 203
ErhI CCWWGG 2 cut(s) 168, 203
Esp3I CGTCTC 2 cut(s) 1649, 2330
FalI AAGNNNNNCTT 2 cut(s) 1935, 1967
FaqI GGGAC 2 cut(s) 1175, 2085
FauI CCCGC 2 cut(s) 281, 1211
FauNDI CATATG 2 cut(s) 264, 527
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 1794
Fnu4HI GCNGC 9 cut(s) 453, 1221, 1518, 1741, 2219, 2280, 2292, 2391, 2517
FokI GGATG 9 cut(s) 158, 312, 330, 490, 602, 1625, 1714, 2254, 2572
Fsp4HI GCNGC 9 cut(s) 453, 1221, 1518, 1741, 2219, 2280, 2292, 2391, 2517
FspBI CTAG 6 cut(s) 1110, 1424, 1521, 2187, 2298, 2427
GlaI GCGC 2 cut(s) 33, 456
GluI GCNGC 9 cut(s) 453, 1221, 1518, 1741, 2219, 2280, 2292, 2391, 2517
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 2 cut(s) 1356, 1380
HaeIII GGCC 4 cut(s) 133, 202, 1956, 2552
HapII CCGG 1 cut(s) 312
HgaI GACGC 2 cut(s) 1319, 1631
HhaI GCGC 2 cut(s) 34, 457
Hin6I GCGC 2 cut(s) 32, 455
HinP1I GCGC 2 cut(s) 32, 455
HindIII AAGCTT 2 cut(s) 1902, 2624
HpaII CCGG 1 cut(s) 312
HphI GGTGA 3 cut(s) 340, 686, 1989
Hpy166II GTNNAC 4 cut(s) 1795, 2055, 2139, 2496
Hpy8I GTNNAC 4 cut(s) 1795, 2055, 2139, 2496
HpyCH4IV ACGT 5 cut(s) 792, 853, 1289, 2212, 2323
HpyF10VI GCNNNNNNNGC 9 cut(s) 208, 452, 817, 1270, 1415, 1526, 1535, 2288, 2396
HpyF3I CTNAG 8 cut(s) 70, 348, 646, 1080, 1401, 1576, 2664, 2681
HpySE526I ACGT 5 cut(s) 792, 853, 1289, 2212, 2323
HspAI GCGC 2 cut(s) 32, 455
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
LguI GCTCTTC 1 cut(s) 1122
LmnI GCTCC 8 cut(s) 80, 385, 553, 817, 1174, 1361, 1423, 1514
Lsp1109I GCAGC 9 cut(s) 464, 1232, 1529, 1752, 2205, 2266, 2278, 2377, 2528
LweI GCATC 8 cut(s) 136, 352, 778, 1708, 2266, 2276, 2310, 2439
MabI ACCWGGT 2 cut(s) 22, 715
MaeI CTAG 6 cut(s) 1110, 1424, 1521, 2187, 2298, 2427
MaeII ACGT 5 cut(s) 792, 853, 1289, 2212, 2323
MaeIII GTNAC 7 cut(s) 289, 328, 430, 788, 1692, 1847, 2569
MalI GATC 6 cut(s) 228, 1619, 1758, 1950, 2380, 2616
MboI GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
MfeI CAATTG 1 cut(s) 697
MflI RGATCY 2 cut(s) 1617, 2614
MhlI GDGCHC 5 cut(s) 608, 1134, 1171, 1540, 2498
MlyI GAGTC 4 cut(s) 317, 638, 1017, 1078
MmeI TCCRAC 3 cut(s) 419, 959, 2370
MroXI GAANNNNTTC 2 cut(s) 1659, 1974
MslI CAYNNNNRTG 2 cut(s) 236, 1164
MspA1I CMGCKG 2 cut(s) 562, 2519
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
MunI CAATTG 1 cut(s) 697
Mva1269I GAATGC 3 cut(s) 1323, 1453, 1742
MvaI CCWGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
MwoI GCNNNNNNNGC 9 cut(s) 208, 452, 817, 1270, 1415, 1526, 1535, 2288, 2396
NcoI CCATGG 1 cut(s) 203
NdeI CATATG 2 cut(s) 264, 527
NdeII GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
NlaIV GGNNCC 8 cut(s) 18, 132, 270, 381, 714, 819, 2101, 2661
NmuCI GTSAC 3 cut(s) 328, 788, 1692
NspI RCATGY 3 cut(s) 1065, 1413, 2242
NspV TTCGAA 1 cut(s) 1663
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 1 cut(s) 1870
PasI CCCWGGG 1 cut(s) 1193
PciI ACATGT 2 cut(s) 1061, 2238
PciSI GCTCTTC 1 cut(s) 1122
PctI GAATGC 3 cut(s) 1323, 1453, 1742
PdmI GAANNNNTTC 2 cut(s) 1659, 1974
PfeI GAWTC 7 cut(s) 656, 707, 879, 950, 1660, 2582, 2594
PflMI CCANNNNNTGG 1 cut(s) 2618
PfoI TCCNGGA 2 cut(s) 491, 1333
PkrI GCNGC 9 cut(s) 454, 1222, 1519, 1742, 2220, 2281, 2293, 2392, 2518
PleI GAGTC 4 cut(s) 316, 638, 1017, 1078
PpsI GAGTC 4 cut(s) 316, 638, 1017, 1078
Ppu21I YACGTR 1 cut(s) 2213
PscI ACATGT 2 cut(s) 1061, 2238
PshAI GACNNNNGTC 1 cut(s) 1062
Psp6I CCWGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
PspN4I GGNNCC 8 cut(s) 18, 132, 270, 381, 714, 819, 2101, 2661
PspPI GGNCC 4 cut(s) 99, 131, 719, 2099
PstI CTGCAG 1 cut(s) 1774
PsuI RGATCY 2 cut(s) 1617, 2614
RsaI GTAC 4 cut(s) 40, 2054, 2138, 2656
RsaNI GTAC 4 cut(s) 39, 2053, 2137, 2655
RseI CAYNNNNRTG 2 cut(s) 236, 1164
SapI GCTCTTC 1 cut(s) 1122
SatI GCNGC 9 cut(s) 453, 1221, 1518, 1741, 2219, 2280, 2292, 2391, 2517
Sau3AI GATC 6 cut(s) 226, 1617, 1756, 1948, 2378, 2614
Sau96I GGNCC 4 cut(s) 99, 131, 719, 2099
SchI GAGTC 4 cut(s) 317, 638, 1017, 1078
ScrFI CCNGG 8 cut(s) 24, 493, 717, 1029, 1194, 1335, 1359, 2001
SduI GDGCHC 5 cut(s) 608, 1134, 1171, 1540, 2498
SexAI ACCWGGT 2 cut(s) 22, 715
SfaNI GCATC 8 cut(s) 136, 352, 778, 1708, 2266, 2276, 2310, 2439
SfcI CTRYAG 3 cut(s) 1770, 1791, 2541
SfuI TTCGAA 1 cut(s) 1663
SinI GGWCC 3 cut(s) 99, 719, 2099
SmiI ATTTAAAT 1 cut(s) 1008
SmiMI CAYNNNNRTG 2 cut(s) 236, 1164
SmlI CTYRAG 5 cut(s) 422, 512, 664, 1634, 1646
SmoI CTYRAG 5 cut(s) 422, 512, 664, 1634, 1646
SsiI CCGC 6 cut(s) 274, 562, 1204, 2198, 2501, 2519
SspI AATATT 2 cut(s) 835, 1673
SspMI CTAG 6 cut(s) 1110, 1424, 1521, 2187, 2298, 2427
StyD4I CCNGG 8 cut(s) 22, 491, 715, 1027, 1192, 1333, 1357, 1999
StyI CCWWGG 2 cut(s) 168, 203
SwaI ATTTAAAT 1 cut(s) 1008
TaiI ACGT 5 cut(s) 795, 856, 1292, 2215, 2326
TaqI TCGA 5 cut(s) 216, 629, 882, 1663, 2433
TatI WGTACW 2 cut(s) 2052, 2136
TfiI GAWTC 7 cut(s) 656, 707, 879, 950, 1660, 2582, 2594
TseFI GTSAC 3 cut(s) 328, 788, 1692
TseI GCWGC 9 cut(s) 452, 1220, 1517, 1740, 2218, 2279, 2291, 2390, 2516
Tsp45I GTSAC 3 cut(s) 328, 788, 1692
Van91I CCANNNNNTGG 1 cut(s) 2618
Vha464I CTTAAG 1 cut(s) 422
VneI GTGCAC 1 cut(s) 2494
VpaK11BI GGWCC 3 cut(s) 99, 719, 2099
XagI CCTNNNNNAGG 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 1423
XceI RCATGY 3 cut(s) 1065, 1413, 2242
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 1794
XmnI GAANNNNTTC 2 cut(s) 1659, 1974
XspI CTAG 6 cut(s) 1110, 1424, 1521, 2187, 2298, 2427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.