Rmu_sc0003163.1_g000002

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003163.1
OG0006341
Physical Location & Seq
Forward (+)
7230 .. 11832
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003163.1_g000002.1.cds

Sequence Viewer

Length: 2772 bp
atgaaaaacaattcggttccacctggttttgcgcctcgtacttccttcacgctgaagagggcagaagtctcagttgtgggagctgaagaaacccataggtccaatgctgttgtggatacttccaagcaagggccaatccagatggatgcaacatctgacatgactgccttggagaaggtcaaaacatttcttcacaataggccatggttgcttttcgaccaaagtgatcacacttcagtgcaatctgacccacaacagtttcatatggtaccccctccgaaaacgtgccccaatggtgttactcgtggaagccctgaatccagttattgtgtaaaggaaccccgcctcccgaaaggtgttactcgtggatgtccagaatccggttattgtgtaaaggtaacagaaaggtggcatcctgagggtgcagtaatagacactcttgaaaaggctcctgttttctatccaacacatgaggaatttaaggacactcttaagtatgttacaagtataagtgcaagagcagcgcaatatggtatctgtcgtattagtcctcctccttcctggaaacctccatcccttcttgaggaaaacagcatatggaatagttccacatttgctccccatattcagcggattgatgggctggggagtaaaatggatggctctgatgagagcacaaaaaagaagagaagaatctcgacagtgggtttggactctggatttgaatctacctcaagtctgggtgaaacaggacagtctgatgtcaattgctttgaatcagaacctggtccagaattcactcttgacagttttaagaggtatgcagatggtttcaagcatcagtatttctgcaaaagtgacgtcataggtaatcaagagcagtgggagccgtcagtggagaatattgaagctgaatataaacgtatcattgaaaacccaactgaaggaatcgaggtgctttgtggaaattatttggagactaaagcgtttggcagtggatttccaacagtatcacatgattccaatcctttgggaacatcagattaccctgattgcttgccatctggctggaatttaaataatctacccagactccctggttctcttctttcttttgaaagccatgacacatgtcatattttagtgcctcagactcatgtaggaatgtgtttttcatctagtctttggagagttgaagagcaccacttatactcattgtgttacaatcatctgggtgctcctaaagtttggtatggtgtccctgggaaatcccgcataaattttgaggcagccatgaggaagtcctttccagatttattaaaacagcataggctggttaagcaagtatccccctccacattgaaatcagaggacatacctgtctttcgttgcattcagcgtcctggagagtttgttcttgtcttccctggagcttatcattcagaatttgattgtggctttaatttttctgagagggcatgttttgctcctctagactggttgcctcatggacagaatgctgttgaactttactctgaacaggggaggaaaacagctatatcctatgataaggtgttgcttggagcagctagggaagctgtaagggcacaatgggaaatttcactgttgaagaagaacaaaaactcagagaatttacgctggaaaaatgcctgcttaaaggatgggatcttaacagaagcactcaagttgcgtctcaagtctgaagagattcgaaggaaatatttttgcacctctttgaagtcacagaggatgaattcagattttgatgctaccatgaaaagggaatgcagcatttgtttctgcgatcttcatttctctgcagtaggttgtccttgttctgtagacagatactcatgtctgtttcatgcaaagcagttctgtttttgtgcttggagtaacaagtttttcctctaccgtcatgaaatcagcgagctaaatctacttgttgaagctttggaaggaaaattaagtgcagtcatcaaatgggcgaaagatgatcttggcctgtatttgcatgaacatcttcccaaaagcaatgcacaatcacctgggcaggtagataatcccatctctcccactgataaaataaagccgaaacagtacacatcggaagatataacgccaccgattttcagttgtggcactggtcccatttatctcactgataaaagaaagctgaaacagtacacattggaagatatatcgccaccgattttgggttttggctctgcttctagcataaaggcggaattgaaggcacgtatgctgcaatccacaatttcaaacatgttgaaagcaaacggtaatccaatagcatcccttgatgctgcaaccagtgctgcttctagcatcagggctgaattgaaggcacgtctcctgcaatcttcaatctccaacacgttgaaaggaaatgacaatccaacaggatcactcaatgctgcgacagccaatggcaatggtagaaattttgcttctagcatcgagttagagatgaaggcacatgaattccaatcaacaatcccaaatgaatataaaggcgtgtgcactccgcaacagccagtggcagcggtagaaactcctattctgcctatagaggtggcctctgatgtttcatctgtaacttcaagtgaatcatcctcggaatctgatgatataataccagatcttggcaagctttcagaagctagtcatcaagcagaaagtacggcacctcagaacattcatattcttagtgatgacagtgatggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

923

Amino Acids

102.28

Weight (kDa)

6.15

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1379
AatII GACGTC 1 cut(s) 864
Acc36I ACCTGC 1 cut(s) 2065
Acc65I GGTACC 1 cut(s) 268
AccB1I GGYRCC 2 cut(s) 268, 2728
AccB7I CCANNNNNTGG 1 cut(s) 2687
AccI GTMKAC 1 cut(s) 1863
AciI CCGC 6 cut(s) 343, 631, 1273, 2267, 2570, 2588
AclWI GGATC 2 cut(s) 1694, 2455
AcuI CTGAAG 5 cut(s) 74, 105, 219, 963, 1743
AcyI GRCGYC 1 cut(s) 861
AfaI GTAC 5 cut(s) 40, 270, 2123, 2207, 2725
AfiI CCNNNNNNNGG 7 cut(s) 129, 380, 583, 944, 1800, 2237, 2687
AflII CTTAAG 1 cut(s) 491
AflIII ACRYGT 3 cut(s) 1130, 2307, 2418
AjiI CACGTC 1 cut(s) 2393
AjnI CCWGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
AleI CACNNNNGTG 1 cut(s) 236
AloI GAACNNNNNNTCC 4 cut(s) 330, 362, 1398, 1430
Alw21I GWGCWC 4 cut(s) 677, 1203, 1240, 2567
Alw26I GTCTC 4 cut(s) 73, 971, 1718, 2399
Alw44I GTGCAC 1 cut(s) 2563
AlwI GGATC 2 cut(s) 1694, 2455
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 4 cut(s) 131, 200, 2023, 2619
ApaLI GTGCAC 1 cut(s) 2563
ApeKI GCWGC 9 cut(s) 521, 1289, 1586, 1809, 2287, 2348, 2360, 2459, 2585
ArsI GACNNNNNNTTYG 6 cut(s) 691, 704, 723, 736, 846, 878
Asp700I GAANNNNTTC 2 cut(s) 1728, 2043
Asp718I GGTACC 1 cut(s) 268
AspLEI GCGC 2 cut(s) 34, 526
AspS9I GGNCC 4 cut(s) 99, 131, 788, 2168
AsuHPI GGTGA 2 cut(s) 755, 2058
AsuII TTCGAA 1 cut(s) 1732
AvaII GGWCC 3 cut(s) 99, 788, 2168
AxyI CCTNAGG 1 cut(s) 417
BaeGI GKGCMC 3 cut(s) 290, 1609, 2567
BaeI ACNNNNGTAYC 2 cut(s) 1861, 1894
BanI GGYRCC 2 cut(s) 268, 2728
BauI CACGAG 2 cut(s) 303, 363
BbsI GAAGAC 1 cut(s) 1414
Bbv12I GWGCWC 4 cut(s) 677, 1203, 1240, 2567
BbvI GCAGC 9 cut(s) 533, 1301, 1598, 1821, 2274, 2335, 2347, 2446, 2597
BccI CCATC 9 cut(s) 136, 580, 632, 653, 821, 1069, 1676, 2096, 2759
BceAI ACGGC 2 cut(s) 874, 2742
BciT130I CCWGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
BciVI GTATCC 2 cut(s) 109, 1357
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 4 cut(s) 73, 971, 1718, 2399
BfaI CTAG 7 cut(s) 1179, 1493, 1590, 2256, 2367, 2496, 2706
BfmI CTRYAG 3 cut(s) 1839, 1860, 2610
BfrI CTTAAG 1 cut(s) 491
BfuAI ACCTGC 1 cut(s) 2065
BfuI GTATCC 2 cut(s) 109, 1357
BglII AGATCT 1 cut(s) 2683
BisI GCNGC 9 cut(s) 522, 1290, 1587, 1810, 2288, 2349, 2361, 2460, 2586
BlsI GCNGC 9 cut(s) 523, 1291, 1588, 1811, 2289, 2350, 2362, 2461, 2587
Bme1390I CCNGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
Bme18I GGWCC 3 cut(s) 99, 788, 2168
BmgBI CACGTC 1 cut(s) 2393
BmgT120I GGNCC 4 cut(s) 99, 131, 788, 2168
BmiI GGNNCC 7 cut(s) 18, 270, 339, 450, 888, 2170, 2730
BmrFI CCNGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
BmsI GCATC 8 cut(s) 136, 421, 847, 1777, 2335, 2345, 2379, 2508
BoxI GACNNNNGTC 1 cut(s) 1131
BpiI GAAGAC 1 cut(s) 1414
BplI GAGNNNNNCTC 2 cut(s) 2606, 2638
BpmI CTGGAG 2 cut(s) 1425, 1449
Bpu14I TTCGAA 1 cut(s) 1732
BpuEI CTTGAG 4 cut(s) 602, 718, 1688, 1700
BsaAI YACGTR 1 cut(s) 2282
BsaBI GATNNNNATC 2 cut(s) 724, 1023
BsaHI GRCGYC 1 cut(s) 861
BsaJI CCNNGG 8 cut(s) 168, 203, 1096, 1261, 1262, 1426, 2069, 2658
BsaWI WCCGGW 1 cut(s) 380
BsaXI ACNNNNNCTCC 8 cut(s) 330, 360, 601, 631, 1077, 1107, 1398, 1428
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 380, 583, 944, 1800, 2237, 2687
Bse1I ACTGG 5 cut(s) 321, 1502, 2170, 2355, 2579
Bse21I CCTNAGG 1 cut(s) 417
Bse3DI GCAATG 2 cut(s) 2062, 2482
Bse8I GATNNNNATC 2 cut(s) 724, 1023
BseBI CCWGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
BseDI CCNNGG 8 cut(s) 168, 203, 1096, 1261, 1262, 1426, 2069, 2658
BseGI GGATG 9 cut(s) 151, 374, 412, 572, 664, 1687, 1776, 2336, 2654
BseJI GATNNNNATC 2 cut(s) 724, 1023
BseLI CCNNNNNNNGG 7 cut(s) 129, 380, 583, 944, 1800, 2237, 2687
BseMI GCAATG 2 cut(s) 2062, 2482
BseMII CTCAG 6 cut(s) 84, 408, 1163, 1461, 1659, 2747
BseNI ACTGG 5 cut(s) 321, 1502, 2170, 2355, 2579
BseRI GAGGAG 2 cut(s) 543, 1479
BseSI GKGCMC 3 cut(s) 290, 1609, 2567
BseXI GCAGC 9 cut(s) 533, 1301, 1598, 1821, 2274, 2335, 2347, 2446, 2597
BseYI CCCAGC 1 cut(s) 643
BsgI GTGCAG 2 cut(s) 444, 2013
BshFI GGCC 4 cut(s) 133, 202, 2025, 2621
BshNI GGYRCC 2 cut(s) 268, 2728
BsiHKAI GWGCWC 4 cut(s) 677, 1203, 1240, 2567
BsiSI CCGG 1 cut(s) 381
BslFI GGGAC 2 cut(s) 1244, 2154
BslI CCNNNNNNNGG 7 cut(s) 129, 380, 583, 944, 1800, 2237, 2687
BsmAI GTCTC 4 cut(s) 73, 971, 1718, 2399
BsmBI CGTCTC 2 cut(s) 1718, 2399
BsmFI GGGAC 2 cut(s) 1244, 2154
BsmI GAATGC 3 cut(s) 1392, 1522, 1811
BsnI GGCC 4 cut(s) 133, 202, 2025, 2621
Bsp119I TTCGAA 1 cut(s) 1732
Bsp1286I GDGCHC 6 cut(s) 290, 677, 1203, 1240, 1609, 2567
Bsp143I GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
Bsp19I CCATGG 1 cut(s) 203
BspACI CCGC 6 cut(s) 343, 631, 1273, 2267, 2570, 2588
BspANI GGCC 4 cut(s) 133, 202, 2025, 2621
BspCNI CTCAG 6 cut(s) 83, 409, 1162, 1462, 1658, 2746
BspHI TCATGA 1 cut(s) 1939
BspLI GGNNCC 7 cut(s) 18, 270, 339, 450, 888, 2170, 2730
BspMAI CTGCAG 1 cut(s) 1843
BspMI ACCTGC 1 cut(s) 2065
BspPI GGATC 2 cut(s) 1694, 2455
BspQI GCTCTTC 1 cut(s) 1191
BspT104I TTCGAA 1 cut(s) 1732
BspT107I GGYRCC 2 cut(s) 268, 2728
BspTI CTTAAG 1 cut(s) 491
BsrDI GCAATG 2 cut(s) 2062, 2482
BsrI ACTGG 5 cut(s) 321, 1502, 2170, 2355, 2579
BssECI CCNNGG 8 cut(s) 168, 203, 1096, 1261, 1262, 1426, 2069, 2658
BssMI GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
BssNI GRCGYC 1 cut(s) 861
BssSI CACGAG 2 cut(s) 303, 363
BssT1I CCWWGG 2 cut(s) 168, 203
Bst2BI CACGAG 2 cut(s) 303, 363
Bst2UI CCWGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
Bst6I CTCTTC 5 cut(s) 50, 680, 1110, 1191, 1719
BstACI GRCGYC 1 cut(s) 861
BstAFI CTTAAG 1 cut(s) 491
BstAPI GCANNNNNTGC 2 cut(s) 1484, 2357
BstBAI YACGTR 1 cut(s) 2282
BstBI TTCGAA 1 cut(s) 1732
BstC8I GCNNGC 4 cut(s) 1058, 1672, 1952, 2693
BstDEI CTNAG 7 cut(s) 70, 417, 1149, 1470, 1645, 2733, 2750
BstDSI CCRYGG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 9 cut(s) 151, 374, 412, 572, 664, 1687, 1776, 2336, 2654
BstHHI GCGC 2 cut(s) 34, 526
BstKTI GATC 6 cut(s) 229, 1689, 1828, 2020, 2450, 2686
BstMAI GTCTC 4 cut(s) 73, 971, 1718, 2399
BstMBI GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
BstMWI GCNNNNNNNGC 9 cut(s) 208, 521, 886, 1339, 1484, 1595, 1604, 2357, 2465
BstNI CCWGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
BstNSI RCATGY 3 cut(s) 1134, 1482, 2311
BstPAI GACNNNNGTC 1 cut(s) 1131
BstSCI CCNGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
BstSFI CTRYAG 3 cut(s) 1839, 1860, 2610
BstSLI GKGCMC 3 cut(s) 290, 1609, 2567
BstV1I GCAGC 9 cut(s) 533, 1301, 1598, 1821, 2274, 2335, 2347, 2446, 2597
BstV2I GAAGAC 1 cut(s) 1414
BstX2I RGATCY 2 cut(s) 1686, 2683
BstXI CCANNNNNNTGG 2 cut(s) 1030, 1068
BstYI RGATCY 2 cut(s) 1686, 2683
Bsu36I CCTNAGG 1 cut(s) 417
BsuI GTATCC 2 cut(s) 109, 1357
BsuRI GGCC 4 cut(s) 133, 202, 2025, 2621
BtgI CCRYGG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 2393
BtsCI GGATG 9 cut(s) 151, 374, 412, 572, 664, 1687, 1776, 2336, 2654
BtsI GCAGTG 2 cut(s) 887, 1000
BveI ACCTGC 1 cut(s) 2065
Cac8I GCNNGC 4 cut(s) 1058, 1672, 1952, 2693
CaiI CAGNNNCTG 1 cut(s) 785
CciI TCATGA 1 cut(s) 1939
CfoI GCGC 2 cut(s) 34, 526
Cfr13I GGNCC 4 cut(s) 99, 131, 788, 2168
CseI GACGC 2 cut(s) 1388, 1700
CsiI ACCWGGT 2 cut(s) 22, 784
Csp6I GTAC 5 cut(s) 39, 269, 2122, 2206, 2724
CspCI CAANNNNNGTGG 4 cut(s) 863, 898, 2217, 2252
CviQI GTAC 5 cut(s) 39, 269, 2122, 2206, 2724
DdeI CTNAG 7 cut(s) 70, 417, 1149, 1470, 1645, 2733, 2750
DpnI GATC 6 cut(s) 228, 1688, 1827, 2019, 2449, 2685
DpnII GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
DraI TTTAAA 1 cut(s) 1077
DrdI GACNNNNNNGTC 1 cut(s) 1379
DseDI GACNNNNNNGTC 1 cut(s) 1379
Eam1104I CTCTTC 5 cut(s) 50, 680, 1110, 1191, 1719
EarI CTCTTC 5 cut(s) 50, 680, 1110, 1191, 1719
EciI GGCGGA 1 cut(s) 2282
Eco130I CCWWGG 2 cut(s) 168, 203
Eco47I GGWCC 3 cut(s) 99, 788, 2168
Eco57I CTGAAG 5 cut(s) 74, 105, 219, 963, 1743
Eco81I CCTNAGG 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoRI GAATTC 3 cut(s) 794, 1774, 2525
EcoRII CCWGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
EcoT14I CCWWGG 2 cut(s) 168, 203
ErhI CCWWGG 2 cut(s) 168, 203
Esp3I CGTCTC 2 cut(s) 1718, 2399
FalI AAGNNNNNCTT 2 cut(s) 2004, 2036
FaqI GGGAC 2 cut(s) 1244, 2154
FauI CCCGC 2 cut(s) 350, 1280
FauNDI CATATG 2 cut(s) 264, 596
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 1863
Fnu4HI GCNGC 9 cut(s) 522, 1290, 1587, 1810, 2288, 2349, 2361, 2460, 2586
FokI GGATG 9 cut(s) 158, 381, 399, 559, 671, 1694, 1783, 2323, 2641
Fsp4HI GCNGC 9 cut(s) 522, 1290, 1587, 1810, 2288, 2349, 2361, 2460, 2586
FspBI CTAG 7 cut(s) 1179, 1493, 1590, 2256, 2367, 2496, 2706
GlaI GCGC 2 cut(s) 33, 525
GluI GCNGC 9 cut(s) 522, 1290, 1587, 1810, 2288, 2349, 2361, 2460, 2586
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 2 cut(s) 1425, 1449
HaeIII GGCC 4 cut(s) 133, 202, 2025, 2621
HapII CCGG 1 cut(s) 381
HgaI GACGC 2 cut(s) 1388, 1700
HhaI GCGC 2 cut(s) 34, 526
Hin1I GRCGYC 1 cut(s) 861
Hin6I GCGC 2 cut(s) 32, 524
HinP1I GCGC 2 cut(s) 32, 524
HindIII AAGCTT 2 cut(s) 1971, 2693
HpaII CCGG 1 cut(s) 381
HphI GGTGA 2 cut(s) 755, 2058
Hpy166II GTNNAC 4 cut(s) 1864, 2124, 2208, 2565
Hpy8I GTNNAC 4 cut(s) 1864, 2124, 2208, 2565
HpyCH4IV ACGT 6 cut(s) 284, 861, 922, 2281, 2392, 2420
HpyF10VI GCNNNNNNNGC 9 cut(s) 208, 521, 886, 1339, 1484, 1595, 1604, 2357, 2465
HpyF3I CTNAG 7 cut(s) 70, 417, 1149, 1470, 1645, 2733, 2750
HpySE526I ACGT 6 cut(s) 284, 861, 922, 2281, 2392, 2420
Hsp92I GRCGYC 1 cut(s) 861
HspAI GCGC 2 cut(s) 32, 524
KpnI GGTACC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
LguI GCTCTTC 1 cut(s) 1191
LmnI GCTCC 8 cut(s) 80, 454, 622, 886, 1243, 1430, 1492, 1583
Lsp1109I GCAGC 9 cut(s) 533, 1301, 1598, 1821, 2274, 2335, 2347, 2446, 2597
LweI GCATC 8 cut(s) 136, 421, 847, 1777, 2335, 2345, 2379, 2508
MabI ACCWGGT 2 cut(s) 22, 784
MaeI CTAG 7 cut(s) 1179, 1493, 1590, 2256, 2367, 2496, 2706
MaeII ACGT 6 cut(s) 284, 861, 922, 2281, 2392, 2420
MaeIII GTNAC 9 cut(s) 298, 358, 397, 499, 857, 1220, 1761, 1916, 2638
MalI GATC 6 cut(s) 228, 1688, 1827, 2019, 2449, 2685
MboI GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
MfeI CAATTG 1 cut(s) 766
MflI RGATCY 2 cut(s) 1686, 2683
MhlI GDGCHC 6 cut(s) 290, 677, 1203, 1240, 1609, 2567
MlyI GAGTC 3 cut(s) 707, 1086, 1147
MmeI TCCRAC 4 cut(s) 488, 1028, 2439, 2465
MroXI GAANNNNTTC 2 cut(s) 1728, 2043
MslI CAYNNNNRTG 2 cut(s) 236, 1233
MspA1I CMGCKG 2 cut(s) 631, 2588
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 1 cut(s) 381
MspR9I CCNGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
MunI CAATTG 1 cut(s) 766
Mva1269I GAATGC 3 cut(s) 1392, 1522, 1811
MvaI CCWGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
MwoI GCNNNNNNNGC 9 cut(s) 208, 521, 886, 1339, 1484, 1595, 1604, 2357, 2465
NcoI CCATGG 1 cut(s) 203
NdeI CATATG 2 cut(s) 264, 596
NdeII GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
NlaIV GGNNCC 7 cut(s) 18, 270, 339, 450, 888, 2170, 2730
NmuCI GTSAC 2 cut(s) 857, 1761
NspI RCATGY 3 cut(s) 1134, 1482, 2311
NspV TTCGAA 1 cut(s) 1732
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 1 cut(s) 1939
PasI CCCWGGG 1 cut(s) 1262
PciI ACATGT 2 cut(s) 1130, 2307
PciSI GCTCTTC 1 cut(s) 1191
PctI GAATGC 3 cut(s) 1392, 1522, 1811
PdmI GAANNNNTTC 2 cut(s) 1728, 2043
PflMI CCANNNNNTGG 1 cut(s) 2687
PfoI TCCNGGA 2 cut(s) 560, 1402
PkrI GCNGC 9 cut(s) 523, 1291, 1588, 1811, 2289, 2350, 2362, 2461, 2587
PleI GAGTC 3 cut(s) 707, 1086, 1147
PpsI GAGTC 3 cut(s) 707, 1086, 1147
Ppu21I YACGTR 1 cut(s) 2282
PscI ACATGT 2 cut(s) 1130, 2307
PshAI GACNNNNGTC 1 cut(s) 1131
Psp6I CCWGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
PspN4I GGNNCC 7 cut(s) 18, 270, 339, 450, 888, 2170, 2730
PspPI GGNCC 4 cut(s) 99, 131, 788, 2168
PstI CTGCAG 1 cut(s) 1843
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 2 cut(s) 1686, 2683
RsaI GTAC 5 cut(s) 40, 270, 2123, 2207, 2725
RsaNI GTAC 5 cut(s) 39, 269, 2122, 2206, 2724
RseI CAYNNNNRTG 2 cut(s) 236, 1233
SapI GCTCTTC 1 cut(s) 1191
SatI GCNGC 9 cut(s) 522, 1290, 1587, 1810, 2288, 2349, 2361, 2460, 2586
Sau3AI GATC 6 cut(s) 226, 1686, 1825, 2017, 2447, 2683
Sau96I GGNCC 4 cut(s) 99, 131, 788, 2168
SchI GAGTC 3 cut(s) 707, 1086, 1147
ScrFI CCNGG 8 cut(s) 24, 562, 786, 1098, 1263, 1404, 1428, 2070
SduI GDGCHC 6 cut(s) 290, 677, 1203, 1240, 1609, 2567
SexAI ACCWGGT 2 cut(s) 22, 784
SfaNI GCATC 8 cut(s) 136, 421, 847, 1777, 2335, 2345, 2379, 2508
SfcI CTRYAG 3 cut(s) 1839, 1860, 2610
SfuI TTCGAA 1 cut(s) 1732
SinI GGWCC 3 cut(s) 99, 788, 2168
SmiI ATTTAAAT 1 cut(s) 1077
SmiMI CAYNNNNRTG 2 cut(s) 236, 1233
SmlI CTYRAG 5 cut(s) 491, 581, 733, 1703, 1715
SmoI CTYRAG 5 cut(s) 491, 581, 733, 1703, 1715
SsiI CCGC 6 cut(s) 343, 631, 1273, 2267, 2570, 2588
SspI AATATT 2 cut(s) 904, 1742
SspMI CTAG 7 cut(s) 1179, 1493, 1590, 2256, 2367, 2496, 2706
StyD4I CCNGG 8 cut(s) 22, 560, 784, 1096, 1261, 1402, 1426, 2068
StyI CCWWGG 2 cut(s) 168, 203
SwaI ATTTAAAT 1 cut(s) 1077
TaiI ACGT 6 cut(s) 287, 864, 925, 2284, 2395, 2423
TaqI TCGA 5 cut(s) 216, 698, 951, 1732, 2502
TatI WGTACW 2 cut(s) 2121, 2205
TseFI GTSAC 2 cut(s) 857, 1761
TseI GCWGC 9 cut(s) 521, 1289, 1586, 1809, 2287, 2348, 2360, 2459, 2585
Tsp45I GTSAC 2 cut(s) 857, 1761
Van91I CCANNNNNTGG 1 cut(s) 2687
Vha464I CTTAAG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 2563
VpaK11BI GGWCC 3 cut(s) 99, 788, 2168
XagI CCTNNNNNAGG 1 cut(s) 581
XbaI TCTAGA 1 cut(s) 1492
XceI RCATGY 3 cut(s) 1134, 1482, 2311
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 1863
XmnI GAANNNNTTC 2 cut(s) 1728, 2043
XspI CTAG 7 cut(s) 1179, 1493, 1590, 2256, 2367, 2496, 2706
ZraI GACGTC 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.