MD05G1195400.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006341
Physical Location & Seq
Forward (+)
32291644 .. 32293977
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1195400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCACATAATTCAGTTCCTCCTGGTTTTGTGTCTCGTACATCCTTCATGCTGAAGAGGGAAAAAACTGCTGAAGAAACTAATAGTTCCAATGCCTTTGTTGATGCTTCTAATGAAGAGCCAGTCCAGCTGGATACGACGTCTGACCTGACTGACATGGATAAGTTGAAAAGACGAAGGCCATGGATACTTTTTGACCAAAGTGATCTCAACACAGAGGAATCTGACTCTGAACACTTTAATACGGACCCCCTTGCAAAATCTTGCCTTCCCAAAGGTGTTACTCACGGATGTCCAGATTGCAGTGGTTGCCGGAAGGTGACAGGAAGGTGGCATCCTGAAGAGGCAAGAACAGATCTCCTTGAAGAAGCACCTCTTTTCTACCCAACAGAAGAGTCCTTCAGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034720 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
JmjC JmjN zf-C5HC2

Protein Analysis

137

Amino Acids

15.39

Weight (kDa)

4.78

Isoelectric Point (pI)

68.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 143
AcuI CTGAAG 4 cut(s) 74, 93, 360, 385
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 1 cut(s) 40
AgsI TTSAA 2 cut(s) 169, 365
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
Alw26I GTCTC 1 cut(s) 39
AoxI GGCC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 24
BciVI GTATCC 2 cut(s) 127, 180
BcoDI GTCTC 1 cut(s) 39
BfuI GTATCC 2 cut(s) 127, 180
BglII AGATCT 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 94, 343
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 3 cut(s) 41, 296, 334
BseNI ACTGG 1 cut(s) 122
BshFI GGCC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 39
BsnI GGCC 1 cut(s) 181
Bsp143I GATC 2 cut(s) 205, 355
Bsp19I CCATGG 1 cut(s) 182
BspANI GGCC 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 249
BspQI GCTCTTC 1 cut(s) 111
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 2 cut(s) 205, 355
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 24
Bst6I CTCTTC 4 cut(s) 50, 111, 336, 387
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 3 cut(s) 41, 296, 334
BstKTI GATC 2 cut(s) 208, 358
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 2 cut(s) 205, 355
BstMWI GCNNNNNNNGC 2 cut(s) 127, 309
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuI GTATCC 2 cut(s) 127, 180
BsuRI GGCC 1 cut(s) 181
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 3 cut(s) 41, 296, 334
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 39
CviAII CATG 3 cut(s) 49, 157, 183
CviJI RGCY 3 cut(s) 121, 130, 181
CviKI_1 RGCY 3 cut(s) 121, 130, 181
CviQI GTAC 1 cut(s) 39
DpnI GATC 2 cut(s) 207, 357
DpnII GATC 2 cut(s) 205, 355
Eam1104I CTCTTC 4 cut(s) 50, 111, 336, 387
EarI CTCTTC 4 cut(s) 50, 111, 336, 387
Eco130I CCWWGG 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 74, 93, 360, 385
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 3 cut(s) 52, 160, 186
FaiI YATR 4 cut(s) 9, 50, 158, 184
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 3 cut(s) 48, 156, 182
FokI GGATG 3 cut(s) 28, 303, 321
HaeIII GGCC 1 cut(s) 181
HapII CCGG 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 3 cut(s) 52, 160, 186
HinfI GANTC 3 cut(s) 221, 227, 395
HpaII CCGG 1 cut(s) 313
HphI GGTGA 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 145, 226, 232
Hpy188III TCNNGA 2 cut(s) 296, 338
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 6 cut(s) 55, 171, 278, 310, 321, 409
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 2 cut(s) 257, 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 309
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 3 cut(s) 52, 160, 186
Kzo9I GATC 2 cut(s) 205, 355
LguI GCTCTTC 1 cut(s) 111
LweI GCATC 2 cut(s) 94, 343
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 2 cut(s) 280, 319
MalI GATC 2 cut(s) 207, 357
MboI GATC 2 cut(s) 205, 355
MboII GAAGA 6 cut(s) 67, 86, 128, 353, 377, 404
MflI RGATCY 1 cut(s) 355
MluCI AATT 1 cut(s) 10
MlyI GAGTC 2 cut(s) 221, 404
MnlI CCTC 5 cut(s) 30, 51, 211, 337, 384
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 127, 309
NcoI CCATGG 1 cut(s) 182
NdeII GATC 2 cut(s) 205, 355
NlaIII CATG 3 cut(s) 52, 160, 186
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 221
PleI GAGTC 2 cut(s) 221, 403
PpsI GAGTC 2 cut(s) 221, 403
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 2 cut(s) 205, 355
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 2 cut(s) 221, 404
ScrFI CCNGG 1 cut(s) 24
SetI ASST 7 cut(s) 132, 143, 150, 280, 321, 332, 376
SfaNI GCATC 2 cut(s) 94, 343
SinI GGWCC 1 cut(s) 247
Sse9I AATT 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 182
TaiI ACGT 1 cut(s) 143
TasI AATT 1 cut(s) 10
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 37, 129
TspGWI ACGGA 2 cut(s) 260, 303
TspRI CASTG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 247
ZraI GACGTC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.