FvH4_2g21520

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004513
Physical Location & Seq
Forward (+)
17885853 .. 17887576
1724 bp
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UTR
Exon/CDS
Intron
FvH4_2g21520.t1

Sequence Viewer

Length: 447 bp
ATGTCAATGTCTTTCAGAGGGCTCAGTCGGCCGAATGCTTCGTGTGCGATGGCTGCGGCGGAGGACAGCAAGAACAGTTTCATGGAGCTGCAGAGGAAGAAGGTTCACCGCTATGTGATATTTAAGGTGGATGAGAAGAAAAGGGAGGTTGTGGTTGAGAAGATTGGTGGTCCGGCTGAGAGTTATGAAGATTTCGTGGCGGCTTTGCCTGAGAATGATTGCCGATTTGCTGTATATGACTTTGATTTTGTTACTTCTGATAACTGTCAAAAGAGCAAGATCTTCTTCATTGCTTGGTCCCCTTCAACCTCTCGGATCCGTGCCAAGATGCTCTATGCCACATCAAAAGACCAATTCAGGCGGGAGCTGGATGGTATTCACTACGAGATTCAGGCTACTGACCCAACAGAGATGGATCTTGAGGTGATCAAAGAGCGGGCGCACTGA

Protein Analysis

149

Amino Acids

17.09

Weight (kDa)

6.32

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 18 - 148 4.7e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 436
AciI CCGC 6 cut(s) 56, 59, 109, 200, 361, 436
AclWI GGATC 3 cut(s) 310, 323, 423
AcoI YGGCCR 1 cut(s) 29
AgsI TTSAA 1 cut(s) 306
AluBI AGCT 2 cut(s) 88, 367
AluI AGCT 2 cut(s) 88, 367
AlwI GGATC 3 cut(s) 310, 323, 423
AoxI GGCC 1 cut(s) 29
ApeKI GCWGC 2 cut(s) 53, 88
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 2 cut(s) 170, 297
AsuHPI GGTGA 2 cut(s) 98, 436
AvaII GGWCC 2 cut(s) 170, 297
BamHI GGATCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 40, 75
BccI CCATC 3 cut(s) 43, 365, 406
BclI TGATCA 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 89
BglII AGATCT 1 cut(s) 279
BisI GCNGC 4 cut(s) 54, 57, 89, 201
BlsI GCNGC 4 cut(s) 55, 58, 90, 202
Bme18I GGWCC 2 cut(s) 170, 297
BmgT120I GGNCC 2 cut(s) 170, 297
BmiI GGNNCC 2 cut(s) 299, 317
BmsI GCATC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 288
BseGI GGATG 2 cut(s) 136, 376
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 3 cut(s) 37, 168, 201
BseX3I CGGCCG 1 cut(s) 29
BseXI GCAGC 2 cut(s) 40, 75
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 32
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 4 cut(s) 279, 315, 415, 426
BspACI CCGC 6 cut(s) 56, 59, 109, 200, 361, 436
BspANI GGCC 1 cut(s) 31
BspCNI CTCAG 3 cut(s) 36, 169, 202
BspLI GGNNCC 2 cut(s) 299, 317
BspMAI CTGCAG 1 cut(s) 93
BspPI GGATC 3 cut(s) 310, 323, 423
BsrBI CCGCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 288
BssMI GATC 4 cut(s) 279, 315, 415, 426
Bst4CI ACNGT 2 cut(s) 77, 266
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 23, 177, 210
BstF5I GGATG 2 cut(s) 136, 376
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 4 cut(s) 282, 318, 418, 429
BstMBI GATC 4 cut(s) 279, 315, 415, 426
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 3 cut(s) 28, 44, 53
BstSFI CTRYAG 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 40, 75
BstX2I RGATCY 3 cut(s) 279, 315, 415
BstYI RGATCY 3 cut(s) 279, 315, 415
BstZI CGGCCG 1 cut(s) 29
BsuRI GGCC 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 136, 376
BtsIMutI CAGTG 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 438
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 170, 297
CviAII CATG 1 cut(s) 82
CviJI RGCY 8 cut(s) 22, 31, 53, 88, 176, 203, 367, 395
CviKI_1 RGCY 8 cut(s) 22, 31, 53, 88, 176, 203, 367, 395
DdeI CTNAG 3 cut(s) 23, 177, 210
DpnI GATC 4 cut(s) 281, 317, 417, 428
DpnII GATC 4 cut(s) 279, 315, 415, 426
EaeI YGGCCR 1 cut(s) 29
EagI CGGCCG 1 cut(s) 29
EciI GGCGGA 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 29
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 170, 297
Eco52I CGGCCG 1 cut(s) 29
EcoT38I GRGCYC 1 cut(s) 24
FaeI CATG 1 cut(s) 85
FaiI YATR 6 cut(s) 83, 114, 186, 235, 237, 336
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 283
FatI CATG 1 cut(s) 81
FauI CCCGC 2 cut(s) 354, 429
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 4 cut(s) 54, 57, 89, 201
FokI GGATG 2 cut(s) 143, 383
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 4 cut(s) 54, 57, 89, 201
GlaI GCGC 1 cut(s) 441
GluI GCNGC 4 cut(s) 54, 57, 89, 201
HaeIII GGCC 1 cut(s) 31
HapII CCGG 1 cut(s) 173
HhaI GCGC 1 cut(s) 442
Hin1II CATG 1 cut(s) 85
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HinfI GANTC 1 cut(s) 388
HpaII CCGG 1 cut(s) 173
HphI GGTGA 2 cut(s) 98, 436
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 3 cut(s) 17, 259, 315
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 2 cut(s) 94, 312
HpyCH4III ACNGT 2 cut(s) 77, 266
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 44, 53
HpyF3I CTNAG 3 cut(s) 23, 177, 210
Hsp92II CATG 1 cut(s) 85
HspAI GCGC 1 cut(s) 440
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 4 cut(s) 279, 315, 415, 426
LmnI GCTCC 2 cut(s) 85, 364
LpnPI CCDG 5 cut(s) 186, 222, 343, 353, 377
Lsp1109I GCAGC 2 cut(s) 40, 75
LweI GCATC 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 281, 317, 417, 428
MbiI CCGCTC 1 cut(s) 436
MboI GATC 4 cut(s) 279, 315, 415, 426
MboII GAAGA 6 cut(s) 109, 148, 172, 200, 274, 277
MflI RGATCY 3 cut(s) 279, 315, 415
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 1 cut(s) 353
MnlI CCTC 6 cut(s) 11, 55, 87, 139, 319, 415
MroXI GAANNNNTTC 1 cut(s) 77
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 111
MspI CCGG 1 cut(s) 173
Mva1269I GAATGC 1 cut(s) 40
MwoI GCNNNNNNNGC 3 cut(s) 28, 44, 53
NdeII GATC 4 cut(s) 279, 315, 415, 426
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 2 cut(s) 299, 317
PctI GAATGC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 4 cut(s) 55, 58, 90, 202
PspN4I GGNNCC 2 cut(s) 299, 317
PspPI GGNCC 2 cut(s) 170, 297
PstI CTGCAG 1 cut(s) 93
PsuI RGATCY 3 cut(s) 279, 315, 415
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 4 cut(s) 54, 57, 89, 201
Sau3AI GATC 4 cut(s) 279, 315, 415, 426
Sau96I GGNCC 2 cut(s) 170, 297
SduI GDGCHC 1 cut(s) 24
SetI ASST 7 cut(s) 90, 105, 129, 150, 311, 369, 426
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 170, 297
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 1 cut(s) 353
SsiI CCGC 6 cut(s) 56, 59, 109, 200, 361, 436
TaaI ACNGT 2 cut(s) 77, 266
TasI AATT 1 cut(s) 353
TauI GCSGC 2 cut(s) 59, 203
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseI GCWGC 2 cut(s) 53, 88
TspDTI ATGAA 3 cut(s) 70, 201, 277
TspGWI ACGGA 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 170, 297
XmnI GAANNNNTTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.