FvH4_4g03580

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004513
Physical Location & Seq
Reverse (-)
3219573 .. 3221448
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03580.t1

Sequence Viewer

Length: 432 bp
ATGGCTTTCAGAGGAGGGAATGCTTCCTCTGGCATGGGTGTTGCTGAGGACTGCCGGAGCACATTCTTGGAACTGCAGAGAAAGAAGGCCTTCCGCTATGTGGTTTTTAAAATTGATGAGAAGAAGAAAGAGGTTGTGGTAGAGAAGACAGGAAGCCCGGCTGAAAGTTATGATGATTTCACTGCTTCGTTGCCTGAAAATGATTGCCGATATGCAATCTACGACTTTGATTTTGTCACTTCAGAGAATTGCCAGAAGAGCAAGATCTTTTTTATTGCATGGTCACCTTCTGTGTCACGGATCCGTGCTAAGATGCTCTATGCGACTTCAAAGGACAGGTTCAGGAGGGAGCTGGATGGTATCCATTATGAGATCCAGGCAACTGACTCCACTGAGATGGAGCTTGAAGTCCTTCAGGAGCGTGCGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

144

Amino Acids

16.51

Weight (kDa)

5.77

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 11 - 143 1.6e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
AciI CCGC 1 cut(s) 94
AclWI GGATC 3 cut(s) 295, 308, 367
AcuI CTGAAG 2 cut(s) 225, 398
AfiI CCNNNNNNNGG 1 cut(s) 100
AgsI TTSAA 2 cut(s) 330, 407
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 2 cut(s) 352, 403
AluI AGCT 2 cut(s) 352, 403
Alw21I GWGCWC 1 cut(s) 62
AlwI GGATC 3 cut(s) 295, 308, 367
AoxI GGCC 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp700I GAANNNNTTC 2 cut(s) 89, 411
AspLEI GCGC 1 cut(s) 427
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 276
BamHI GGATCC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 62
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 350, 391
BciT130I CCWGG 1 cut(s) 377
BciVI GTATCC 1 cut(s) 371
BcnI CCSGG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 74
BfuI GTATCC 1 cut(s) 371
BglII AGATCT 1 cut(s) 264
Bme1390I CCNGG 2 cut(s) 158, 377
BmiI GGNNCC 1 cut(s) 302
BmrFI CCNGG 2 cut(s) 158, 377
BmsI GCATC 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 377
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 2 cut(s) 36, 384
BseRI GAGGAG 1 cut(s) 27
BshFI GGCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 2 cut(s) 55, 158
BslI CCNNNNNNNGG 1 cut(s) 100
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 3 cut(s) 264, 300, 372
BspACI CCGC 1 cut(s) 94
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 2 cut(s) 37, 385
BspLI GGNNCC 1 cut(s) 302
BspMAI CTGCAG 1 cut(s) 78
BspPI GGATC 3 cut(s) 295, 308, 367
BspQI GCTCTTC 1 cut(s) 251
BssMI GATC 3 cut(s) 264, 300, 372
Bst2UI CCWGG 1 cut(s) 377
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 3 cut(s) 45, 309, 393
BstEII GGTNACC 1 cut(s) 282
BstF5I GGATG 1 cut(s) 361
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 3 cut(s) 267, 303, 375
BstMBI GATC 3 cut(s) 264, 300, 372
BstMWI GCNNNNNNNGC 1 cut(s) 258
BstNI CCWGG 1 cut(s) 377
BstPI GGTNACC 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 156, 375
BstSFI CTRYAG 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 3 cut(s) 264, 300, 372
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 3 cut(s) 264, 300, 372
BsuI GTATCC 1 cut(s) 371
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 3 cut(s) 180, 390, 427
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 1 cut(s) 427
CviAII CATG 2 cut(s) 34, 279
CviJI RGCY 6 cut(s) 5, 89, 156, 161, 352, 403
CviKI_1 RGCY 6 cut(s) 5, 89, 156, 161, 352, 403
DdeI CTNAG 3 cut(s) 45, 309, 393
DpnI GATC 3 cut(s) 266, 302, 374
DpnII GATC 3 cut(s) 264, 300, 372
DraI TTTAAA 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco147I AGGCCT 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 225, 398
Eco91I GGTNACC 1 cut(s) 282
EcoO65I GGTNACC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 375
FaeI CATG 2 cut(s) 37, 282
FaiI YATR 7 cut(s) 35, 99, 171, 213, 280, 321, 369
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 2 cut(s) 33, 278
FokI GGATG 1 cut(s) 368
FspAI RTGCGCAY 1 cut(s) 426
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
HaeIII GGCC 1 cut(s) 89
HapII CCGG 2 cut(s) 55, 158
HhaI GCGC 1 cut(s) 427
Hin1II CATG 2 cut(s) 37, 282
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 1 cut(s) 386
HpaII CCGG 2 cut(s) 55, 158
HphI GGTGA 1 cut(s) 276
Hpy188I TCNGA 2 cut(s) 11, 244
Hpy188III TCNNGA 2 cut(s) 343, 416
HpyAV CCTTC 4 cut(s) 79, 100, 297, 422
HpyCH4V TGCA 3 cut(s) 76, 215, 278
HpyF10VI GCNNNNNNNGC 1 cut(s) 258
HpyF3I CTNAG 3 cut(s) 45, 309, 393
Hsp92II CATG 2 cut(s) 37, 282
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 3 cut(s) 264, 300, 372
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 4 cut(s) 57, 349, 400, 418
LweI GCATC 1 cut(s) 303
MaeIII GTNAC 3 cut(s) 235, 282, 294
MalI GATC 3 cut(s) 266, 302, 374
MboI GATC 3 cut(s) 264, 300, 372
MboII GAAGA 4 cut(s) 133, 136, 157, 268
MflI RGATCY 3 cut(s) 264, 300, 372
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 2 cut(s) 111, 247
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 6 cut(s) 5, 8, 37, 40, 124, 339
MroXI GAANNNNTTC 2 cut(s) 89, 411
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 2 cut(s) 55, 158
MspR9I CCNGG 2 cut(s) 158, 377
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 258
NciI CCSGG 1 cut(s) 158
NdeII GATC 3 cut(s) 264, 300, 372
NlaIII CATG 2 cut(s) 37, 282
NlaIV GGNNCC 1 cut(s) 302
NmuCI GTSAC 3 cut(s) 235, 282, 294
NsbI TGCGCA 1 cut(s) 426
PceI AGGCCT 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 1 cut(s) 25
PdmI GAANNNNTTC 2 cut(s) 89, 411
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 375
PspEI GGTNACC 1 cut(s) 282
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 78
PsuI RGATCY 3 cut(s) 264, 300, 372
RseI CAYNNNNRTG 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 108
Sau3AI GATC 3 cut(s) 264, 300, 372
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 2 cut(s) 158, 377
SduI GDGCHC 1 cut(s) 62
SetI ASST 5 cut(s) 135, 289, 341, 354, 405
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 2 cut(s) 111, 247
SseBI AGGCCT 1 cut(s) 89
SsiI CCGC 1 cut(s) 94
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 156, 375
TasI AATT 2 cut(s) 111, 247
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 2 cut(s) 187, 397
TseFI GTSAC 3 cut(s) 235, 282, 294
Tsp45I GTSAC 3 cut(s) 235, 282, 294
TspGWI ACGGA 2 cut(s) 293, 313
TspRI CASTG 2 cut(s) 187, 397
XmnI GAANNNNTTC 2 cut(s) 89, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.