Rorug03G0341500

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004513
Physical Location & Seq
Reverse (-)
41000739 .. 41002041
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0341500.1

Sequence Viewer

Length: 945 bp
ATGATGAGGATAGAGACCTCATTCTTGGCCTTTTTCTTTCTTCTTCCTCTAGCAGTGGCTGACTTGCGGGTTGGTTTCTACAGTGCTACTTGCCCAAAAGCCGAACAAATTGTACAACAAGTCGTCCAGAAAAAATTCTCCAGTGATCGATCTATAACTGCTGCTTTGCTTCGCATGCACTTCCATGACTGCTTTGTTAGAGGTTGTGATGCTTCCATCCTTATTGACTCCACCAAGAAGAAGGCGTCTGAAAAAACCGCAGGACCAAACTTGACTGTAAGAGGGTTTGAGGTCATCGACGAGGCCAAGAAAGCACTTGAGGCTGCATGCCCGTCAACAGTCTCCTGTGCTGATATCATAACCCTCGCGACTAGAGATTCAGTGGTTCTAGCCGGTGGCCCGAGCTACAACTCACCCACCGGACGGCGTGACGGCCTAGTATCGAATCCCAATGACGTCAACTTACCTGGTCCGTCCTTCTCCGTGTCTCAGGCATTGCAGTCTTTCACGGCCAAAGGAATGACTCTGAACGATATGGTGACTCTTTTGGGTGCACACACCGTTGGCGTTGCGCACTGTAGCTTTTTTGAAGATAGGCTTTCCAACTTTCAGGGGACTGGGTCTCCCGATCCTTCAATGGACCCAGCTCTGGTTACCAAGCTGAAAAAGATCTGTGGAGGACCTAATGACCCTACTGCATTTTTGGACCAAAATACATCATTTGCCTTTGATAATCAATTCTATAACCAGGTTAATTTGAAGAGAGGTGTGATGCAGATTGACCAGGAGCTAGGTGTGGACAAGTCAACGGCTGGTATTGTTTCTGGGTTTGCTTCCAATGGTGGCAGATTCAGTCAGAGTTTTGCAACTGCTATGTTGAAGATGGGCAGCCTTCAGGATGGAAATGCTGGTGAAATCAGGAAAAATTGTAGAGCTTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

314

Amino Acids

33.69

Weight (kDa)

8.09

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 38 - 280 5.5e-87 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
Acc16I TGCGCA 1 cut(s) 573
AccII CGCG 1 cut(s) 368
AciI CCGC 2 cut(s) 67, 258
AclWI GGATC 1 cut(s) 623
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 878
AcyI GRCGYC 2 cut(s) 245, 456
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 423, 649
AgsI TTSAA 4 cut(s) 590, 636, 760, 880
AjnI CCWGG 3 cut(s) 466, 747, 783
AluBI AGCT 6 cut(s) 405, 582, 647, 661, 790, 935
AluI AGCT 6 cut(s) 405, 582, 647, 661, 790, 935
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 4 cut(s) 8, 346, 492, 627
Alw44I GTGCAC 1 cut(s) 552
AlwI GGATC 1 cut(s) 623
AlwNI CAGNNNCTG 1 cut(s) 59
Ama87I CYCGRG 1 cut(s) 400
AoxI GGCC 5 cut(s) 27, 303, 397, 433, 510
ApaLI GTGCAC 1 cut(s) 552
ApeKI GCWGC 3 cut(s) 161, 323, 888
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 6 cut(s) 263, 398, 470, 640, 680, 706
AsuHPI GGTGA 3 cut(s) 405, 550, 923
AvaI CYCGRG 1 cut(s) 400
AvaII GGWCC 5 cut(s) 263, 470, 640, 680, 706
BaeGI GKGCMC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 3 cut(s) 148, 310, 900
BccI CCATC 3 cut(s) 224, 877, 893
BceAI ACGGC 4 cut(s) 440, 448, 525, 825
BciT130I CCWGG 3 cut(s) 468, 749, 785
BcoDI GTCTC 4 cut(s) 8, 346, 492, 627
BfaI CTAG 5 cut(s) 50, 372, 389, 437, 791
BfmI CTRYAG 2 cut(s) 79, 577
BglII AGATCT 1 cut(s) 669
BisI GCNGC 3 cut(s) 162, 324, 889
BlsI GCNGC 3 cut(s) 163, 325, 890
Bme1390I CCNGG 3 cut(s) 468, 749, 785
Bme18I GGWCC 5 cut(s) 263, 470, 640, 680, 706
BmeT110I CYCGRG 1 cut(s) 400
BmgT120I GGNCC 6 cut(s) 263, 398, 470, 640, 680, 706
BmiI GGNNCC 1 cut(s) 642
BmrFI CCNGG 3 cut(s) 468, 749, 785
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 2 cut(s) 199, 762
BmuI ACTGGG 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 148
BsaHI GRCGYC 2 cut(s) 245, 456
BsaI GGTCTC 2 cut(s) 8, 627
BsaWI WCCGGW 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 607, 637
Bsc4I CCNNNNNNNGG 2 cut(s) 423, 649
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 141, 622
Bse3DI GCAATG 1 cut(s) 494
BseBI CCWGG 3 cut(s) 468, 749, 785
BseCI ATCGAT 1 cut(s) 148
BseGI GGATG 2 cut(s) 216, 904
BseLI CCNNNNNNNGG 2 cut(s) 423, 649
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 2 cut(s) 141, 622
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 3 cut(s) 148, 310, 900
BseYI CCCAGC 1 cut(s) 643
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 5 cut(s) 29, 305, 399, 435, 512
BshVI ATCGAT 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 400
BsiSI CCGG 2 cut(s) 393, 420
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 2 cut(s) 423, 649
BsmAI GTCTC 4 cut(s) 8, 346, 492, 627
BsmFI GGGAC 1 cut(s) 628
BsnI GGCC 5 cut(s) 29, 305, 399, 435, 512
Bso31I GGTCTC 2 cut(s) 8, 627
BsoBI CYCGRG 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 112
Bsp143I GATC 4 cut(s) 145, 149, 628, 669
Bsp68I TCGCGA 1 cut(s) 368
BspACI CCGC 2 cut(s) 67, 258
BspANI GGCC 5 cut(s) 29, 305, 399, 435, 512
BspCNI CTCAG 1 cut(s) 502
BspDI ATCGAT 1 cut(s) 148
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 1 cut(s) 642
BspPI GGATC 1 cut(s) 623
BspTNI GGTCTC 2 cut(s) 8, 627
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 392
BsrGI TGTACA 1 cut(s) 112
BsrI ACTGG 2 cut(s) 141, 622
BssAI RCCGGY 1 cut(s) 392
BssMI GATC 4 cut(s) 145, 149, 628, 669
BssNI GRCGYC 2 cut(s) 245, 456
Bst2UI CCWGG 3 cut(s) 468, 749, 785
Bst4CI ACNGT 5 cut(s) 83, 277, 340, 562, 578
Bst6I CTCTTC 1 cut(s) 755
BstACI GRCGYC 2 cut(s) 245, 456
BstAUI TGTACA 1 cut(s) 112
BstC8I GCNNGC 2 cut(s) 176, 328
BstDEI CTNAG 1 cut(s) 489
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 2 cut(s) 216, 904
BstFNI CGCG 1 cut(s) 368
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 4 cut(s) 148, 152, 631, 672
BstMAI GTCTC 4 cut(s) 8, 346, 492, 627
BstMBI GATC 4 cut(s) 145, 149, 628, 669
BstMWI GCNNNNNNNGC 3 cut(s) 175, 311, 320
BstNI CCWGG 3 cut(s) 468, 749, 785
BstNSI RCATGY 2 cut(s) 178, 330
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 466, 747, 783
BstSFI CTRYAG 2 cut(s) 79, 577
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 3 cut(s) 148, 310, 900
BstX2I RGATCY 1 cut(s) 669
BstYI RGATCY 1 cut(s) 669
Bsu15I ATCGAT 1 cut(s) 148
BsuRI GGCC 5 cut(s) 29, 305, 399, 435, 512
BsuTUI ATCGAT 1 cut(s) 148
BtsCI GGATG 2 cut(s) 216, 904
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 88, 148, 387, 574
BtuMI TCGCGA 1 cut(s) 368
Cac8I GCNNGC 2 cut(s) 176, 328
CaiI CAGNNNCTG 1 cut(s) 59
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 6 cut(s) 263, 398, 470, 640, 680, 706
ClaI ATCGAT 1 cut(s) 148
CseI GACGC 1 cut(s) 234
CsiI ACCWGGT 2 cut(s) 466, 747
Csp6I GTAC 1 cut(s) 113
CviAII CATG 3 cut(s) 175, 185, 327
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 1 cut(s) 489
DpnI GATC 4 cut(s) 147, 151, 630, 671
DpnII GATC 4 cut(s) 145, 149, 628, 669
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
Eco31I GGTCTC 2 cut(s) 8, 627
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 5 cut(s) 263, 470, 640, 680, 706
Eco57I CTGAAG 1 cut(s) 878
Eco88I CYCGRG 1 cut(s) 400
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 680
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 3 cut(s) 466, 747, 783
EcoRV GATATC 1 cut(s) 355
FaeI CATG 3 cut(s) 178, 188, 330
FaiI YATR 8 cut(s) 155, 176, 186, 328, 359, 536, 744, 875
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 1 cut(s) 628
FatI CATG 3 cut(s) 174, 184, 326
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 162, 324, 889
FokI GGATG 2 cut(s) 203, 911
Fsp4HI GCNGC 3 cut(s) 162, 324, 889
FspBI CTAG 5 cut(s) 50, 372, 389, 437, 791
FspI TGCGCA 1 cut(s) 573
GlaI GCGC 1 cut(s) 573
GluI GCNGC 3 cut(s) 162, 324, 889
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 5 cut(s) 29, 305, 399, 435, 512
HapII CCGG 2 cut(s) 393, 420
HgaI GACGC 1 cut(s) 234
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 2 cut(s) 245, 456
Hin1II CATG 3 cut(s) 178, 188, 330
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HincII GTYRAC 3 cut(s) 336, 460, 807
HindII GTYRAC 3 cut(s) 336, 460, 807
HinfI GANTC 6 cut(s) 227, 377, 445, 523, 541, 849
HpaII CCGG 2 cut(s) 393, 420
HphI GGTGA 3 cut(s) 405, 550, 923
Hpy166II GTNNAC 5 cut(s) 336, 460, 554, 799, 807
Hpy188I TCNGA 3 cut(s) 250, 528, 858
Hpy188III TCNNGA 5 cut(s) 127, 367, 626, 896, 919
Hpy8I GTNNAC 5 cut(s) 336, 460, 554, 799, 807
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 4 cut(s) 235, 487, 642, 902
HpyCH4III ACNGT 5 cut(s) 83, 277, 340, 562, 578
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 7 cut(s) 178, 326, 499, 554, 698, 775, 866
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 311, 320
HpyF3I CTNAG 1 cut(s) 489
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 2 cut(s) 245, 456
Hsp92II CATG 3 cut(s) 178, 188, 330
HspAI GCGC 1 cut(s) 572
Kzo9I GATC 4 cut(s) 145, 149, 628, 669
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 3 cut(s) 148, 310, 900
LweI GCATC 2 cut(s) 199, 762
MabI ACCWGGT 2 cut(s) 466, 747
MaeI CTAG 5 cut(s) 50, 372, 389, 437, 791
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 3 cut(s) 428, 538, 652
MalI GATC 4 cut(s) 147, 151, 630, 671
MboI GATC 4 cut(s) 145, 149, 628, 669
MboII GAAGA 6 cut(s) 32, 35, 250, 602, 772, 892
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 6 cut(s) 108, 134, 737, 754, 925, 940
MlyI GAGTC 3 cut(s) 221, 517, 535
MmeI TCCRAC 1 cut(s) 627
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 3 cut(s) 753, 939, 943
MslI CAYNNNNRTG 1 cut(s) 183
MspI CCGG 2 cut(s) 393, 420
MspR9I CCNGG 3 cut(s) 468, 749, 785
MvaI CCWGG 3 cut(s) 468, 749, 785
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 175, 311, 320
NdeII GATC 4 cut(s) 145, 149, 628, 669
NlaIII CATG 3 cut(s) 178, 188, 330
NlaIV GGNNCC 1 cut(s) 642
NmuCI GTSAC 2 cut(s) 428, 538
NruI TCGCGA 1 cut(s) 368
NsbI TGCGCA 1 cut(s) 573
NspI RCATGY 2 cut(s) 178, 330
PacI TTAATTAA 1 cut(s) 943
PaeI GCATGC 2 cut(s) 178, 330
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 3 cut(s) 377, 445, 849
PflFI GACNNNGTC 1 cut(s) 619
PkrI GCNGC 3 cut(s) 163, 325, 890
PleI GAGTC 3 cut(s) 221, 517, 535
PpsI GAGTC 3 cut(s) 221, 517, 535
PpuMI RGGWCCY 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 3 cut(s) 466, 747, 783
PspEI GGTNACC 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 3 cut(s) 466, 747, 783
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 6 cut(s) 263, 398, 470, 640, 680, 706
PspPPI RGGWCCY 1 cut(s) 680
PsrI GAACNNNNNNTAC 2 cut(s) 96, 128
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 1 cut(s) 669
PsyI GACNNNGTC 1 cut(s) 619
RruI TCGCGA 1 cut(s) 368
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 3 cut(s) 753, 939, 943
SatI GCNGC 3 cut(s) 162, 324, 889
Sau3AI GATC 4 cut(s) 145, 149, 628, 669
Sau96I GGNCC 6 cut(s) 263, 398, 470, 640, 680, 706
SchI GAGTC 3 cut(s) 221, 517, 535
ScrFI CCNGG 3 cut(s) 468, 749, 785
SduI GDGCHC 1 cut(s) 556
SexAI ACCWGGT 2 cut(s) 466, 747
SfaNI GCATC 2 cut(s) 199, 762
SfcI CTRYAG 2 cut(s) 79, 577
SinI GGWCC 5 cut(s) 263, 470, 640, 680, 706
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 2 cut(s) 178, 330
Sse9I AATT 6 cut(s) 108, 134, 737, 754, 925, 940
SsiI CCGC 2 cut(s) 67, 258
SspMI CTAG 5 cut(s) 50, 372, 389, 437, 791
StyD4I CCNGG 3 cut(s) 466, 747, 783
TaaI ACNGT 5 cut(s) 83, 277, 340, 562, 578
TaiI ACGT 1 cut(s) 459
TaqI TCGA 3 cut(s) 148, 297, 443
TasI AATT 6 cut(s) 108, 134, 737, 754, 925, 940
TatI WGTACW 1 cut(s) 112
TfiI GAWTC 3 cut(s) 377, 445, 849
Tru1I TTAA 3 cut(s) 753, 939, 943
Tru9I TTAA 3 cut(s) 753, 939, 943
TscAI CASTG 5 cut(s) 60, 88, 148, 387, 581
TseFI GTSAC 2 cut(s) 428, 538
TseI GCWGC 3 cut(s) 161, 323, 888
Tsp45I GTSAC 2 cut(s) 428, 538
TspGWI ACGGA 2 cut(s) 462, 472
TspRI CASTG 5 cut(s) 60, 88, 148, 387, 581
Tth111I GACNNNGTC 1 cut(s) 619
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 5 cut(s) 263, 470, 640, 680, 706
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 178, 330
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 5 cut(s) 50, 372, 389, 437, 791
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.