MD05G1197900.v1.1

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004513
Physical Location & Seq
Forward (+)
32475975 .. 32477949
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1197900.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCTGCAATGTCTTTCAAAGGGCTCGGTCGGCCAAATGCTTCATGTGCCATGGGGGTTTCCGATGAGAGCAAGAACACTTTCATGGAGCTCCAGAGGAAGAAAGTTCACCGCTATGTGATATTCAAGGTTGATGAGAAGAAGAGGGAGGTTGTAGTTGAAAAAATTGGCGGTCCGGCGGAGAGCTATGATGATTTTGTGGCAGCTTTGCCGGATAATGATTGTCGATATGCCGTATATGACTTTGATTTCGTAACTTCTGAGAACTGTCAAAAGAGCAAGATCTTCTTCATCGCATGGTCCCCTTCAACCTCTCGAATCCGTGCGAAGATGCTCTATGCCACATCTAAAAACAGGTTTAGGAGGGAGCTGGACGGTATCCACTACGAGATTCAGGCTACTGACCCAACAGAGATGGATCTTGAGGTGCTCAAAGACCGCGCCCATTGA

Protein Analysis

150

Amino Acids

17.15

Weight (kDa)

7.69

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 18 - 149 2.9e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 441
AciI CCGC 4 cut(s) 112, 171, 179, 439
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 32
AgsI TTSAA 4 cut(s) 19, 127, 161, 309
AluBI AGCT 4 cut(s) 91, 186, 206, 370
AluI AGCT 4 cut(s) 91, 186, 206, 370
Alw21I GWGCWC 2 cut(s) 93, 432
AlwI GGATC 1 cut(s) 426
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 2 cut(s) 5, 203
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 173, 300
AsuHPI GGTGA 1 cut(s) 101
AvaII GGWCC 2 cut(s) 173, 300
BanII GRGCYC 2 cut(s) 27, 93
Bbv12I GWGCWC 2 cut(s) 93, 432
BbvI GCAGC 1 cut(s) 215
BccI CCATC 1 cut(s) 409
BceAI ACGGC 1 cut(s) 218
BciVI GTATCC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 389
BglII AGATCT 1 cut(s) 282
BisI GCNGC 2 cut(s) 6, 204
BlsI GCNGC 2 cut(s) 7, 205
Bme18I GGWCC 2 cut(s) 173, 300
BmgT120I GGNCC 2 cut(s) 173, 300
BmiI GGNNCC 1 cut(s) 302
BmsI GCATC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 252
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 93, 432
BsiSI CCGG 2 cut(s) 176, 212
BslFI GGGAC 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 27, 93, 432
Bsp143I GATC 2 cut(s) 282, 418
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 4 cut(s) 112, 171, 179, 439
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 302
BspPI GGATC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 282, 418
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 269, 377
Bst6I CTCTTC 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 51
BstFNI CGCG 1 cut(s) 441
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 2 cut(s) 285, 421
BstMBI GATC 2 cut(s) 282, 418
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 2 cut(s) 31, 47
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 282, 418
BstYI RGATCY 2 cut(s) 282, 418
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 277
CfoI GCGC 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 173, 300
CpoI CGGWCCG 1 cut(s) 173
CspI CGGWCCG 1 cut(s) 173
CviAII CATG 4 cut(s) 45, 52, 85, 297
CviJI RGCY 8 cut(s) 5, 25, 34, 91, 186, 206, 370, 398
CviKI_1 RGCY 8 cut(s) 5, 25, 34, 91, 186, 206, 370, 398
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 284, 420
DpnII GATC 2 cut(s) 282, 418
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 27, 93
Eco47I GGWCC 2 cut(s) 173, 300
Eco53kI GAGCTC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 27, 93
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 48, 55, 88, 300
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 286
FatI CATG 4 cut(s) 44, 51, 84, 296
Fnu4HI GCNGC 2 cut(s) 6, 204
FriOI GRGCYC 2 cut(s) 27, 93
Fsp4HI GCNGC 2 cut(s) 6, 204
GlaI GCGC 1 cut(s) 442
GluI GCNGC 2 cut(s) 6, 204
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 34
HapII CCGG 2 cut(s) 176, 212
HhaI GCGC 1 cut(s) 443
Hin1II CATG 4 cut(s) 48, 55, 88, 300
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 2 cut(s) 318, 391
HpaII CCGG 2 cut(s) 176, 212
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 64, 262
Hpy188III TCNNGA 3 cut(s) 94, 315, 422
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 315
HpyCH4III ACNGT 2 cut(s) 269, 377
HpyCH4V TGCA 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 47
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 4 cut(s) 48, 55, 88, 300
HspAI GCGC 1 cut(s) 441
Kzo9I GATC 2 cut(s) 282, 418
LmnI GCTCC 3 cut(s) 88, 96, 367
LpnPI CCDG 6 cut(s) 107, 189, 225, 340, 356, 380
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 284, 420
MboI GATC 2 cut(s) 282, 418
MboII GAAGA 6 cut(s) 112, 151, 154, 277, 280, 340
MflI RGATCY 2 cut(s) 282, 418
MhlI GDGCHC 3 cut(s) 27, 93, 432
MluCI AATT 1 cut(s) 165
MnlI CCTC 6 cut(s) 90, 138, 142, 322, 357, 418
MroXI GAANNNNTTC 1 cut(s) 80
MslI CAYNNNNRTG 2 cut(s) 83, 114
MspI CCGG 2 cut(s) 176, 212
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 2 cut(s) 31, 47
NcoI CCATGG 1 cut(s) 51
NdeII GATC 2 cut(s) 282, 418
NlaIII CATG 4 cut(s) 48, 55, 88, 300
NlaIV GGNNCC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 2 cut(s) 318, 391
PkrI GCNGC 2 cut(s) 7, 205
Psp124BI GAGCTC 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 302
PspPI GGNCC 2 cut(s) 173, 300
PsuI RGATCY 2 cut(s) 282, 418
RseI CAYNNNNRTG 2 cut(s) 83, 114
Rsr2I CGGWCCG 1 cut(s) 173
RsrII CGGWCCG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 93
SatI GCNGC 2 cut(s) 6, 204
Sau3AI GATC 2 cut(s) 282, 418
Sau96I GGNCC 2 cut(s) 173, 300
SduI GDGCHC 3 cut(s) 27, 93, 432
SetI ASST 9 cut(s) 93, 132, 153, 188, 208, 314, 359, 372, 429
SfaNI GCATC 1 cut(s) 321
SinI GGWCC 2 cut(s) 173, 300
SmiMI CAYNNNNRTG 2 cut(s) 83, 114
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 1 cut(s) 165
SsiI CCGC 4 cut(s) 112, 171, 179, 439
SstI GAGCTC 1 cut(s) 93
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 2 cut(s) 269, 377
TaqI TCGA 2 cut(s) 226, 316
TaqII GACCGA 1 cut(s) 17
TasI AATT 1 cut(s) 165
TfiI GAWTC 2 cut(s) 318, 391
TseI GCWGC 2 cut(s) 5, 203
TspDTI ATGAA 3 cut(s) 33, 73, 280
TspGWI ACGGA 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 173, 300
XmnI GAANNNNTTC 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.